LOCUS       sequence-305988-       14545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes p40S:QfDBD-QF2AD-GFP-NLS synthetic transcription factor, 
            activating DsRed-SKL with promoter 5xQUAS+CP_ura3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14545)
  AUTHORS   Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
  TITLE     Synthetic control devices for gene regulation in Penicillium 
            chrysogenum.
  JOURNAL   Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 
            10.1186/s12934-019-1253-3.
  PUBMED    31739777
REFERENCE   2  (bases 1 to 14545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2019-11-18"; volume: "18(1):203. doi"; pages: " 
            10.1186/s12934-019-1253-3"
COMMENT     Sequence Label: pLM2_34_p40S_QfDBD_Qf2AD_cp_ura3_DsRed_5xQUAS
FEATURES             Location/Qualifiers
     source          1..14545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3270..3272,3273..3275,3276..3986)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3270..3272,3273..3275,3276..3986)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3315..3336)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3576..3595)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3923..3944
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3987..4007
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          5179..5294
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             7815..8489
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(7945..7963)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7995..8015)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8407..8430
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    10323..10347
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      10435..11145
                     /label=oriV
                     /note="incP origin of replication"
     promoter        11166..11270
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11271..12065
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12236..12824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12725..12744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             13160..14308
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14454..14478
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
       61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
      121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
      181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
      241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
      301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
      361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
      421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
      481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
      541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
      601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
      661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
      721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
      781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
      841 gaattcaagc ttggaggagt tatagacggt ccggcatagg taaggagaga ttagatattt
      901 cgacttcccg attccttagt ctccttagtc tggaaactga gaaaggcctg aactcgttca
      961 atctattagc tctaatcacg tagtctcaac tagaatcctg aacaggcgcc ggaaccagtc
     1021 caaatgcgtg gagtacagca agtccagtca aatcacgatt agaatggcca tatataagca
     1081 aaaaaccctt ggacaatacg tccgatcgac accaaagcaa tctggtacat cacccttctt
     1141 atatacaggg tagccggaat tgtattgact ctatacgaag ccgcaggttt accagcacag
     1201 aggtgcgata gctacacgaa tgtagagtga aggcattaca accgattgct aagtgtcata
     1261 gaggctaagc ttcacggctc aaggagtctg tggaggatac ctgatataag aattgagagt
     1321 tatactccgg aggttatgtt agtacctcaa aaatgtgaag tacaacgaaa ctcgaacttc
     1381 gaatgacaat agcttggctg gctgttagtc aactgaggta caacgtcatc ttgcaattag
     1441 ttatgttctt tgctagatag tgagctgctg taaacttgta gttcttagaa acatgtctga
     1501 agtctagagt cgagacttgt gattggtgta aattagtggg ggtccggagc ggaataacaa
     1561 tcgaggtcag cctcctgggt ctgtcagtaa gctaaccacg aaaggacaag cgtagacgag
     1621 gtcacggggt actggcctag ccggcttcgg ctagcgccca cgaaaatagg gcaacgtcga
     1681 agcgagagac acacacggat tccacgatcg ccgacgacaa gcactcaact ccacgacaac
     1741 ccacgacaac ccttgccgct gcgttcttgg tgaagggctg caggattaga ggtgagtatt
     1801 ttattttctg gctattgtga tctttttccc ccccggtttc cgctggttct atcttgtcga
     1861 gcgagtcggt gcgccttcga tcggtctgtc aaatttccat ccgtccactc gttcacccgg
     1921 tatcgataca agagggaatg gagcattacg acaggggagc agaggcagaa ctctcgatgt
     1981 tgctactttt tttgcttgga acaacttcct tgaaagattc ttttctgaat gcggctggga
     2041 aactgcgaat tggatatcca gcgaaaggtt accgacagac tcacggccat cgataccact
     2101 cagcgggata gaattgaagg cattcggaca ttagatgatc agcttcgctt gtttcgactc
     2161 gtgctaatca attatccctg gacagtcccc cacatagaca gcaaacaatg ccacccaagc
     2221 gcaaaacgct taacgctgcg gctgaggcta acgctcatgc cgacggacac gccgacggaa
     2281 acgccgacgg acacgtggcc aatacggccg cgtcctcgaa taatgcgagg ttcgctgatc
     2341 tcactaacat cgatactccg ggtctgggac ccacaactac gaccctgctc gtggaaccag
     2401 cacgctcaaa gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca gcccgagaga
     2461 aatgcgacgg aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg tcctgcactt
     2521 atcaggcttc gccgaaaaag aggggagttc aaaccggtta tattcgtacg ctggagctcg
     2581 ccctcgcctg gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat aacctcctcg
     2641 tccgtgacgc cggacaagga tcagctctgc tcgttggtaa agattcgccg gctgccgagc
     2701 gactccatgc ccgttgggct actagccgtg tcaataagag cattacccgc ctcctcaggt
     2761 ttcgtcagtt ggagctccct cctaccgcca cggctacggc ctcgataatg ccgcacgtga
     2821 tggagcagcc tctcagtacc agcattaacc ccgtcaacga ccgcttcaac ggtattccca
     2881 accccactcc gtataactcc gatgcagctc tcgatgctat cactcagacc aacgattatg
     2941 gaagcgtaaa tacacatggt atcctctcta cttacccgcc accggctacg caccttaatg
     3001 aagcttccgt cgctctcgct cccggtggcg cccccccccg accgcctcct ccgtatgttg
     3061 acagcacgac caatcacccg ccgtaccact cgaatctggt tccaatggcg aactttggtt
     3121 actcgaccgt tgattacgat gccatggttg acgatttggc tagcattgaa tacacggacg
     3181 ctgtggatgt cgacccacag tttatgacca atctgggatt cgttcctgga tgtaacttct
     3241 ccgacattaa tacatacgaa cagttcgtta tggtgagcaa gggcgaggag ctgttcaccg
     3301 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     3361 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     3421 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     3481 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     3541 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     3601 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     3661 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     3721 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     3781 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     3841 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     3901 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3961 ctctcggcat ggacgagctg tacaagccaa agaagaagcg gaaggtctaa gcttacttct
     4021 ttatcggttc tctcttacga ctttttgaat ggaacgtttc cttcttctca ggcgggccta
     4081 tctttgggcc gaagctcttt tccttgtact gtaggacctg gttgataatg attcccaaaa
     4141 agacatccag catgtcagtt acttgcattc gtcagtctat acaaaagcaa tggtttagag
     4201 aaattttgaa ctttatacat ggttttattt gttgcttcac ggccgtacct tctggaaatc
     4261 cacggtagga gtgtcaattt gcgtttttga taatccttcc aaggttcttc tcgaagtagt
     4321 tgttctataa ttgcttcaca gctaccatgg aacatcccaa gcaccgctac tagaattcga
     4381 gctcggagtg gatcccccgg gctgcaggaa ttcgagctct gtacagtgac cggtgactct
     4441 ttctggcatg cggagagacg gacggacgca gagagaaggg ctgagtaata agcgccactg
     4501 cgccagacag ctctggcggc tctgaggtgc agtggatgat tattaatccg ggaccggccg
     4561 cccctccgcc ccgaagtgga aaggctggtg tgcccctcgt tgaccaagaa tctattgcat
     4621 catcggagaa tatggagctt catcgaatca ccggcagtaa gcgaaggaga atgtgaagcc
     4681 aggggtgtat agccgtcggc gaaatagcat gccattaacc taggtacaga agtccaattg
     4741 cttccgatct ggtaaaagat tcacgagata gtaccttctc cgaagtaggt agagcgagta
     4801 cccggcgcgt aagctcccta attggcccat ccggcatctg tagggcgtcc aaatatcgtg
     4861 cctctcctgc tttgcccggt gtatgaaacc ggaaaggccg ctcaggagct ggccagcggc
     4921 gcagaccggg aacacaagct ggcagtcgac ccatccggtg ctctgcactc gacctgctga
     4981 ggtccctcag tccctggtag gcagctttgc cccgtctgtc cgcccggtgt gtcggcgggg
     5041 ttgacaaggt cgttgcgtca gtccaacatt tgttgccata ttttcctgct ctccccacca
     5101 gctgctcttt tcttttctct ttcttttccc atcttcagta tattcatctt cccatccaag
     5161 aacctttatt tcccctaagt aagtactttg ctacatccat actccatcct tcccatccct
     5221 tattcctttg aacctttcag ttcgagcttt cccacttcat cgcagcttga ctaacagcta
     5281 ccccgcttga gcagacatca ccatgcctca atcctgggaa gaactggccg ctgataagcg
     5341 cgcccgcctc gcaaaaacca tccctgatga atggaaagtc cagacgctgc ctgcggatga
     5401 cagcgttatt gatttcccaa agaaatcggg gatcctttca gaggccgaac tgaagatcac
     5461 agaggcctcc gctgcagatc ttgtgtccaa gctggcggcc ggagagttga cctcggtgga
     5521 agttacgcta gcattctgta aacgggcagc aatcgcccag cagttagtag ggtcccctct
     5581 acctctcagg gagatgtaac aacgccacct tatgggacta tcaagctgac gctggcttct
     5641 gtgcagacaa actgcgccca cgagttcttc cctgacgccg ctctcgcgca ggcaagggaa
     5701 ctcgatgaat actacgcaaa gcacaagaca cccgttggtc cactccatgg cctccccatc
     5761 tctctcaaag accagcttcg agtcaaggta caccgttgcc cctaagtcgt tagatgtccc
     5821 tttttgtcag ctaacatatg ccaccagggc tacgaaacat caatgggcta catctcatgg
     5881 ctaaacaagt acgacgaagg ggactcggtt ctgacaacca tgctccgcaa agccggtgcc
     5941 gttttctacg tcaagacctc tgtcccgcag accctgatgg tctgcgagac agtcaacaac
     6001 atcatcgggc gcaccgtcaa cccacgcaac aagaactggt cgtgcggcgg cagttctggt
     6061 ggtgagggtg cgatcgttgg gattcgtggt ggcgtcatcg gtgtaggaac ggatatcggt
     6121 ggctcgattc gagtgccggc cgcgttcaac ttcctgtacg gtctaaggcc gagtcatggg
     6181 cggctgccgt atgcaaagat ggcgaacagc atggagggtc aggagacggt gcacagcgtt
     6241 gtcgggccga ttacgcactc tgttgagggt gagtccttcg cctcttcctt cttttcctgc
     6301 tctataccag gcctccactg tcctcctttc ttgcttttta tactatatac gagacgggca
     6361 gtcactgatg aagtaatgtt agacctccgc ctcttcacca aatccgtcct cggtcaggag
     6421 ccatggaaat acgactccaa ggtcatcccc atgccctggc gccagtccga gtcggacatt
     6481 attgcctcca agatcaagaa cggcgggctc aatatcggct actacaactt cgacggcaat
     6541 gtccttccac accctcctat cctgcgcggc gtggaaacca ccgtcgccgc actcgccaaa
     6601 gccggtcaca ccgtgacccc gtggacgcca tacaagcacg atttcggcca cgatctcatc
     6661 tcccatatct acgcggctga cggcagcgcc gacgtaatgc gcgatatcag tgcatccggc
     6721 gagccggcga ttccaaatat caaagaccta ctgaacccga acatcaaagc tgttaacatg
     6781 aacgagctct gggacacgca tctccagaag tggaattacc agatggagta ccttgagaaa
     6841 tggcgggagg ctgaagaaaa ggccgggaag gaactggacg ccatcatcgc gccgattacg
     6901 cctaccgctg cggtacggca tgaccagttc cggtactatg ggtatgcctc tgtgatcaac
     6961 ctgctggatt tcacgagcgt ggttgttccg gttacctttg cggataagaa catcgataag
     7021 aagaatgaga gtttcaaggc ggttagtgag cttgatgccc tcgtgcagga agagtatgat
     7081 ccggaggcgt accatggggc accggttgca gtgcaggtta tcggacggag actcagtgaa
     7141 gagaggacgt tggcgattgc agaggaagtg gggaagttgc tgggaaatgt ggtgactcca
     7201 taggcttcta ataagtgtca gatagcaatt tgcacaagaa atcaatacca gcaactgtaa
     7261 ataagcgctg aagtgaccat gccatgctac gaaagagcag aaaaaaacct gccgtagaac
     7321 cgaagagata tgacacgctt ccatctctca aaggaagaat cccttcaggg ttgcgtttcc
     7381 agtctagaca cgtataacgg cacaagtgtc tctcaccaaa tgggttatat ctcaaatgtg
     7441 atctaaggat ggaaagccca gaatattggc tgggttgatg gctgcttcga gtgcagtctc
     7501 atgctgccac aggtgactct ggatggcccc ataccactca acccatggta cgagcggtac
     7561 gctttacgaa ttcccatggg gaggggtaat cgattatcct cggataaaca cttatcccag
     7621 ggtaatcact tatccctgga taaacaatta tcctcgggta atcgcttatc cccatcagaa
     7681 ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat gtggtgttga
     7741 agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa cctgcaggaa
     7801 acgaagataa atcaatggcc tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg
     7861 tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     7921 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
     7981 tcgcctggga catcctgtcc ccccagttcc agtacggctc caaggtgtac gtgaagcacc
     8041 ccgccgacat ccccgactac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
     8101 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     8161 gctccttcat ctacaaggtg aagttcatcg gcgtgaactt cccctccgac ggccccgtaa
     8221 tgcagaagaa aactatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg
     8281 tgctgaaggg cgagatccac aaggccctga agctgaagga cggcggccac tacctggtgg
     8341 agttcaagtc catctacatg gccaagaagc ccgtgcagct gcccggctac tactacgtgg
     8401 actccaagct ggacatcacc tcccacaacg aggactacac catcgtggag cagtacgagc
     8461 gcgccgaggg ccgccaccac ctgttcctga ctcacggtat ggacgaattg tacaagtcga
     8521 agctgtaagc ttgtgcttct aaggtatgag tcgcaaaatt gttttttatt tttggtcttg
     8581 agtctaatat gctcgcagct cttgcgttgt atatggtcgt tgtcgcgtat tttctgttgt
     8641 attaaaagat caaacgagat caagggatgg ctcgcgggct gtctctcgca ctaggaggaa
     8701 gaatgcctga aaaaggaact ttgattttag ctgtggaata gagatggctt gtttgaggac
     8761 gcttgtcgct tggcgcaggg acttgaatgg cagcttgtgg aaaccgaagg cgagaaaagt
     8821 cgacggatac tgtacgtggt tctattgcca gtgcggtgga agcttccttc tcatatagtt
     8881 caatccttct ttgaatctgt ttgtttcata tttggactgt ttcattctct gcttgcgcat
     8941 tctcatcttc gagaacgact gcagggattg ttggttctgt ggagctgatg agcgcgcctt
     9001 gaccaccctt gttcttgttt tgctcttttg ttctcattta acccgtttct cccttccaac
     9061 cctttgacct tgcaacattg tctcccagcg cgttgccaaa gcgaacttga tatcagtata
     9121 gtatgaccaa gtagtctacc aaaataaatt ttagtacagt attgctagtt atacaagata
     9181 aattttgtac aacaatgatt atgcagagct ggtattctgt aaggagtaaa ttcatgcagg
     9241 tacagagcgt ccacgtcagg cacacgcgct ttagtgctgg gtacacatta tgctaccttt
     9301 ggggcaaggt gctattctag gtagggtatg cctagcaatg ccatgatctt atgacctcag
     9361 taatctgacc ttggatttaa gatcggagtt ctcaaaccct gcgcgctcag agaattcgca
     9421 tgcggagact ttagacatac ctctcgttgc cgacaaatcg gcattccgtt tcggtcacgg
     9481 acgtaagagt tcaacaaagc cgctcacctg tcccgttcgg cgcttgagtt tccacttcaa
     9541 aagcacaacc gctccgatat ttccattgcc accgaccaac aaaaggttca tatcgcgcaa
     9601 tagtcgtcca actctcactc cgcccgcact cgatggcgaa ggatggaaga atatccgagc
     9661 ggcatcggat aaagagatct ggttcttttc gtgaagtaac gtagggaacc gacggaaact
     9721 gtagagctat aggaatttag tatatcagaa gtcaattgag agaagtgtat agttagtaac
     9781 tcacttgtgc caacaggtat ttccaaccgt tcttgttcaa atttcgtcaa gtcaccatct
     9841 ctatgtgcaa aactggatca tacaacagct cttattgtag ctgcatctcg ctctcagaga
     9901 tcatcctatc ttcgtcgtca tccaccaaca caggtgggag tgctagttga tccatgctga
     9961 ggtcaaggga cacagacgga atgggtgtag aacgcctcaa aacaggtaag gtatcaggat
    10021 cggttgtcca gggggaatcc aaaccaggtt ccaccttgat tcagagaata agtatgacgt
    10081 attcttggca atgtttaagc ttgagatgag ccgatgtcaa actgggagac atgatgaagg
    10141 aggaaatgtt gctttggtgg gggagacact ataaatacca aagagtgaat ggttgatacc
    10201 atttggggaa ggagtgtcta tcgcagtctc tgcagacggc atatcggata gcctgcaggc
    10261 gcttgtggag gatgcacatg tgaccgaggg acacgaagtg atccgtttaa actatcagtg
    10321 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
    10381 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc cagccgcctt
    10441 tgcgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct atggccctgc
    10501 aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cgcggccgcc ggcgttgtgg
    10561 atacctcgcg gaaaacttgg ccctcactga cagatgaggg gcggacgttg acacttgagg
    10621 ggccgactca cccggcgcgg cgttgacaga tgaggggcag gctcgatttc ggccggcgac
    10681 gtggagctgg ccagcctcgc aaatcggcga aaacgcctga ttttacgcga gtttcccaca
    10741 gatgatgtgg acaagcctgg ggataagtgc cctgcggtat tgacacttga ggggcgcgac
    10801 tactgacaga tgaggggcgc gatccttgac acttgagggg cagagtgctg acagatgagg
    10861 ggcgcaccta ttgacatttg aggggctgtc cacaggcaga aaatccagca tttgcaaggg
    10921 tttccgcccg tttttcggcc accgctaacc tgtcttttaa cctgctttta aaccaatatt
    10981 tataaacctt gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg
    11041 gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc gggcctccca tccccccagg
    11101 ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat ggcagcgctg gccaattccc
    11161 gagtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    11221 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atggctaaaa
    11281 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
    11341 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
    11401 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
    11461 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
    11521 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
    11581 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
    11641 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
    11701 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaggacactc
    11761 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
    11821 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
    11881 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
    11941 gcgtccggtc gctcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
    12001 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
    12061 tttagctgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    12121 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    12181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    12241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    12301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    12361 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    12421 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    12481 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    12541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    12601 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    12661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    12721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    12781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    12841 cctttttacg gttcctggca gatcctagat gtggcgcaac gatgccggcg acaagcagga
    12901 gcgcaccgac ttcttccgca tcaagtgttt tggctctcag gccgaggccc acggcaagta
    12961 tttgggcaag gggtcgctgg tattcgtgca gggcaagatt cggaatacca agtacgagaa
    13021 ggacggccag acggtctacg ggaccgactt cattgccgat aaggtggatt atctggacac
    13081 caaggcacca ggcgggtcaa atcaggaata agggcacatt gccccggcgt gagtcggggc
    13141 aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg aaggcataca ggcaagaact
    13201 gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc gcaagccgca ccgtcatgcg
    13261 tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta cggccaagat
    13321 cgagcgcgac agcgtgcaac tggctccccc tgccctgccc gcgccatcgg ccgccgtgga
    13381 gcgttcgcgt cgtcttgaac aggaggcggc aggtttggcg aagtcgatga ccatcgacac
    13441 gcgaggaact atgacgacca agaagcgaaa aaccgccggc gaggacctgg caaaacaggt
    13501 cagcgaggcc aagcaggccg cgttgctgaa acacacgaag cagcagatca aggaaatgca
    13561 gctttccttg ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc caaacgacac
    13621 ggcccgctct gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg cgctgcaaaa
    13681 caaggtcatt ttccacgtca acaaggacgt gaagatcacc tacaccggcg tcgagctgcg
    13741 ggccgacgat gacgaactgg tgtggcagca ggtgttggag tacgcgaagc gcacccctat
    13801 cggcgagccg atcaccttca cgttctacga gctttgccag gacctgggct ggtcgatcaa
    13861 tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga cggcgatggg
    13921 cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc gcttccgcgt
    13981 cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg aaatcgtcgt
    14041 gctgtttgct ggcgaccact acacgaaatt catatgggag aagtaccgca agctgtcgcc
    14101 gacggcccga cggatgttcg actatttcag ctcgcaccgg gagccgtacc cgctcaagct
    14161 ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt ggcgcgagca
    14221 ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg gtggaacacg cctgggtcaa
    14281 tgatgacctg gtgcattgca aacgctaggg ccttgtgggg tcagttccgg ctgggggttc
    14341 agcagcccct gctcggatct gttggaccgg acagtagtca tggttgatgg gctgcctgta
    14401 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
    14461 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt
    14521 ttaatgtact ggggttgaac actct
//
