LOCUS       sequence-305989-       14241 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, 
            activating DsRed-SKL with promoter 5xQUAS(reversed)+CP_pcbC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14241)
  AUTHORS   Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
  TITLE     Synthetic control devices for gene regulation in Penicillium 
            chrysogenum.
  JOURNAL   Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 
            10.1186/s12934-019-1253-3.
  PUBMED    31739777
REFERENCE   2  (bases 1 to 14241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2019-11-18"; volume: "18(1):203. doi"; pages: " 
            10.1186/s12934-019-1253-3"
COMMENT     Sequence Label: pVE2_4_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS(...
FEATURES             Location/Qualifiers
     source          1..14241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2781..2891
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2949..2970)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3210..3229)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3557..3578
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3621..3641
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4813..4928
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             7511..8185
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(7641..7659)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7691..7711)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8103..8126
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    10019..10043
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      10131..10841
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10862..10966
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10967..11761
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11932..12520
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12421..12440
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12856..14004
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14150..14174
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
       61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
      121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
      181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
      241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
      301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
      361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
      421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
      481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
      541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
      601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
      661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
      721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
      781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
      841 gaattcaagc ttggaggagt tatagacggt ccggcatagg taaggagaga ttagatattt
      901 cgacttcccg attccttagt ctccttagtc tggaaactga gaaaggcctg aactcgttca
      961 atctattagc tctaatcacg tagtctcaac tagaatcctg aacaggcgcc ggaaccagtc
     1021 caaatgcgtg gagtacagca agtccagtca aatcacgatt agaatggcca tatataagca
     1081 aaaaaccctt ggacaatacg tccgatcgac accaaagcaa tctggtacat cacccttctt
     1141 atatacaggg tagccggaat tgtattgact ctatacgaag ccgcaggttt accagcacag
     1201 aggtgcgata gctacacgaa tgtagagtga aggcattaca accgattgct aagtgtcata
     1261 gaggctaagc ttcacggctc aaggagtctg tggaggatac ctgatataag aattgagagt
     1321 tatactccgg aggttatgtt agtacctcaa aaatgtgaag tacaacgaaa ctcgaacttc
     1381 gaatgacaat agcttggctg gctgttagtc aactgaggta caacgtcatc ttgcaattag
     1441 ttatgttctt tgctagatag tgagctgctg taaacttgta gttcttagaa acatgtctga
     1501 agtctagagt cgagacttgt gattggtgta aattagtggg ggtccggagc ggaataacaa
     1561 tcgaggtcag cctcctgggt ctgtcagtaa gctaaccacg aaaggacaag cgtagacgag
     1621 gtcacggggt actggcctag ccggcttcgg ctagcgccca cgaaaatagg gcaacgtcga
     1681 agcgagagac acacacggat tccacgatcg ccgacgacaa gcactcaact ccacgacaac
     1741 ccacgacaac ccttgccgct gcgttcttgg tgaagggctg caggattaga ggtgagtatt
     1801 ttattttctg gctattgtga tctttttccc ccccggtttc cgctggttct atcttgtcga
     1861 gcgagtcggt gcgccttcga tcggtctgtc aaatttccat ccgtccactc gttcacccgg
     1921 tatcgataca agagggaatg gagcattacg acaggggagc agaggcagaa ctctcgatgt
     1981 tgctactttt tttgcttgga acaacttcct tgaaagattc ttttctgaat gcggctggga
     2041 aactgcgaat tggatatcca gcgaaaggtt accgacagac tcacggccat cgataccact
     2101 cagcgggata gaattgaagg cattcggaca ttagatgatc agcttcgctt gtttcgactc
     2161 gtgctaatca attatccctg gacagtcccc cacatagaca gcaaacaatg ccacccaagc
     2221 gcaaaacgct taacgctgcg gctgaggcta acgctcatgc cgacggacac gccgacggaa
     2281 acgccgacgg acacgtggcc aatacggccg cgtcctcgaa taatgcgagg ttcgctgatc
     2341 tcactaacat cgatactccg ggtctgggac ccacaactac gaccctgctc gtggaaccag
     2401 cacgctcaaa gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca gcccgagaga
     2461 aatgcgacgg aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg tcctgcactt
     2521 atcaggcttc gccgaaaaag aggggagttc aaaccggtta tattcgtacg ctggagctcg
     2581 ccctcgcctg gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat aacctcctcg
     2641 tccgtgacgc cggacaagga tcagctctgc tcgttggtaa agattcgccg gctgccgagc
     2701 gactccatgc ccgttgggct actagccgtg tcaataagag cattacccgc ctcctcaggt
     2761 ttgaaagcgg cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg
     2821 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     2881 ttgacatgct ccccgggttc gttatggtga gcaagggcga ggagctgttc accggggtgg
     2941 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     3001 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     3061 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     3121 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3181 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3241 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3301 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3361 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3421 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3481 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3541 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3601 gcatggacga gctgtacaag ccaaagaaga agcggaaggt ctaagcttac ttctttatcg
     3661 gttctctctt acgacttttt gaatggaacg tttccttctt ctcaggcggg cctatctttg
     3721 ggccgaagct cttttccttg tactgtagga cctggttgat aatgattccc aaaaagacat
     3781 ccagcatgtc agttacttgc attcgtcagt ctatacaaaa gcaatggttt agagaaattt
     3841 tgaactttat acatggtttt atttgttgct tcacggccgt accttctgga aatccacggt
     3901 aggagtgtca atttgcgttt ttgataatcc ttccaaggtt cttctcgaag tagttgttct
     3961 ataattgctt cacagctacc atggaacatc ccaagcaccg ctactagaat tcgagctcgg
     4021 agtggatccc ccgggctgca ggaattcgag ctctgtacag tgaccggtga ctctttctgg
     4081 catgcggaga gacggacgga cgcagagaga agggctgagt aataagcgcc actgcgccag
     4141 acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc
     4201 cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg
     4261 agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt
     4321 gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg
     4381 atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc
     4441 gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc
     4501 ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac
     4561 cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc
     4621 tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca
     4681 aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct
     4741 cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt
     4801 tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc
     4861 tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc
     4921 ttgagcagac atcaccatgc ctcaatcctg ggaagaactg gccgctgata agcgcgcccg
     4981 cctcgcaaaa accatccctg atgaatggaa agtccagacg ctgcctgcgg atgacagcgt
     5041 tattgatttc ccaaagaaat cggggatcct ttcagaggcc gaactgaaga tcacagaggc
     5101 ctccgctgca gatcttgtgt ccaagctggc ggccggagag ttgacctcgg tggaagttac
     5161 gctagcattc tgtaaacggg cagcaatcgc ccagcagtta gtagggtccc ctctacctct
     5221 cagggagatg taacaacgcc accttatggg actatcaagc tgacgctggc ttctgtgcag
     5281 acaaactgcg cccacgagtt cttccctgac gccgctctcg cgcaggcaag ggaactcgat
     5341 gaatactacg caaagcacaa gacacccgtt ggtccactcc atggcctccc catctctctc
     5401 aaagaccagc ttcgagtcaa ggtacaccgt tgcccctaag tcgttagatg tccctttttg
     5461 tcagctaaca tatgccacca gggctacgaa acatcaatgg gctacatctc atggctaaac
     5521 aagtacgacg aaggggactc ggttctgaca accatgctcc gcaaagccgg tgccgttttc
     5581 tacgtcaaga cctctgtccc gcagaccctg atggtctgcg agacagtcaa caacatcatc
     5641 gggcgcaccg tcaacccacg caacaagaac tggtcgtgcg gcggcagttc tggtggtgag
     5701 ggtgcgatcg ttgggattcg tggtggcgtc atcggtgtag gaacggatat cggtggctcg
     5761 attcgagtgc cggccgcgtt caacttcctg tacggtctaa ggccgagtca tgggcggctg
     5821 ccgtatgcaa agatggcgaa cagcatggag ggtcaggaga cggtgcacag cgttgtcggg
     5881 ccgattacgc actctgttga gggtgagtcc ttcgcctctt ccttcttttc ctgctctata
     5941 ccaggcctcc actgtcctcc tttcttgctt tttatactat atacgagacg ggcagtcact
     6001 gatgaagtaa tgttagacct ccgcctcttc accaaatccg tcctcggtca ggagccatgg
     6061 aaatacgact ccaaggtcat ccccatgccc tggcgccagt ccgagtcgga cattattgcc
     6121 tccaagatca agaacggcgg gctcaatatc ggctactaca acttcgacgg caatgtcctt
     6181 ccacaccctc ctatcctgcg cggcgtggaa accaccgtcg ccgcactcgc caaagccggt
     6241 cataccgtga ccccgtggac gccatacaag cacgatttcg gccacgatct catctcccat
     6301 atctacgcgg ctgacggcag cgccgacgta atgcgcgata tcagtgcatc cggcgagccg
     6361 gcgattccaa atatcaaaga cctactgaac ccgaacatca aagctgttaa catgaacgag
     6421 ctctgggaca cgcatctcca gaagtggaat taccagatgg agtaccttga gaaatggcgg
     6481 gaggctgaag aaaaggccgg gaaggaactg gacgccatca tcgcgccgat tacgcctacc
     6541 gctgcggtac ggcatgacca gttccggtac tatgggtatg cctctgtgat caacctgctg
     6601 gatttcacga gcgtggttgt tccggttacc tttgcggata agaacatcga taagaagaat
     6661 gagagtttca aggcggttag tgagcttgat gccctcgtgc aggaagagta tgatccggag
     6721 gcgtaccatg gggcaccggt tgcagtgcag gttatcggac ggagactcag tgaagagagg
     6781 acgttggcga ttgcagagga agtggggaag ttgctgggaa atgtggtgac tccataggct
     6841 tctaataagt gtcagatagc aatttgcaca agaaatcaat accagcaact gtaaataagc
     6901 gctgaagtga ccatgccatg ctacgaaaga gcagaaaaaa acctgccgta gaaccgaaga
     6961 gatatgacac gcttccatct ctcaaaggaa gaatcccttc agggttgcgt ttccagtcta
     7021 gacacgtata acggcacaag tgtctctcac caaatgggtt atatctcaaa tgtgatctaa
     7081 ggatggaaag cccagaatat tggctgggtt gatggctgct tcgagtgcag tctcatgctg
     7141 ccacaggtga ctctggatgg ccccatacca ctcaacccat ggtacgagcg gtacgcttta
     7201 cgaattccca tggggaggga taagcgatta cccgaggata agcgattacc cagggataag
     7261 cgattaccct gggataattg tttatccgag gataagcgat tacccccatc gtataatgtc
     7321 tccaggttgt ctcagcataa acaccccgcc cccgctcagg cacacaggaa gagagctcag
     7381 gtcgtttcca ttgcgtccat actcttcact cattgtcatc tgcaggagaa cttcccctgt
     7441 ccctttgcca agccctctct tcgtcgttgt ccacgccttc aagttttcac cattattttt
     7501 ctagacacca atggcctcct ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg
     7561 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     7621 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     7681 ctgggacatc ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc
     7741 cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     7801 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggctc
     7861 cttcatctac aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtaatgca
     7921 gaagaaaact atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
     7981 gaagggcgag atccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
     8041 caagtccatc tacatggcca agaagcccgt gcagctgccc ggctactact acgtggactc
     8101 caagctggac atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcgc
     8161 cgagggccgc caccacctgt tcctgactca cggtatggac gaattgtaca agtcgaagct
     8221 gtaagcttgt gcttctaagg tatgagtcgc aaaattgttt tttatttttg gtcttgagtc
     8281 taatatgctc gcagctcttg cgttgtatat ggtcgttgtc gcgtattttc tgttgtatta
     8341 aaagatcaaa cgagatcaag ggatggctcg cgggctgtct ctcgcactag gaggaagaat
     8401 gcctgaaaaa ggaactttga ttttagctgt ggaatagaga tggcttgttt gaggacgctt
     8461 gtcgcttggc gcagggactt gaatggcagc ttgtggaaac cgaaggcgag aaaagtcgac
     8521 ggatactgta cgtggttcta ttgccagtgc ggtggaagct tccttctcat atagttcaat
     8581 ccttctttga atctgtttgt ttcatatttg gactgtttca ttctctgctt gcgcattctc
     8641 atcttcgaga acgactgcag ggattgttgg ttctgtggag ctgatgagcg cgccttgacc
     8701 acccttgttc ttgttttgct cttttgttct catttaaccc gtttctccct tccaaccctt
     8761 tgaccttgca acattgtctc ccagcgcgtt gccaaagcga acttgatatc agtatagtat
     8821 gaccaagtag tctaccaaaa taaattttag tacagtattg ctagttatac aagataaatt
     8881 ttgtacaaca atgattatgc agagctggta ttctgtaagg agtaaattca tgcaggtaca
     8941 gagcgtccac gtcaggcaca cgcgctttag tgctgggtac acattatgct acctttgggg
     9001 caaggtgcta ttctaggtag ggtatgccta gcaatgccat gatcttatga cctcagtaat
     9061 ctgaccttgg atttaagatc ggagttctca aaccctgcgc gctcagagaa ttcgcatgcg
     9121 gagactttag acatacctct cgttgccgac aaatcggcat tccgtttcgg tcacggacgt
     9181 aagagttcaa caaagccgct cacctgtccc gttcggcgct tgagtttcca cttcaaaagc
     9241 acaaccgctc cgatatttcc attgccaccg accaacaaaa ggttcatatc gcgcaatagt
     9301 cgtccaactc tcactccgcc cgcactcgat ggcgaaggat ggaagaatat ccgagcggca
     9361 tcggataaag agatctggtt cttttcgtga agtaacgtag ggaaccgacg gaaactgtag
     9421 agctatagga atttagtata tcagaagtca attgagagaa gtgtatagtt agtaactcac
     9481 ttgtgccaac aggtatttcc aaccgttctt gttcaaattt cgtcaagtca ccatctctat
     9541 gtgcaaaact ggatcataca acagctctta ttgtagctgc atctcgctct cagagatcat
     9601 cctatcttcg tcgtcatcca ccaacacagg tgggagtgct agttgatcca tgctgaggtc
     9661 aagggacaca gacggaatgg gtgtagaacg cctcaaaaca ggtaaggtat caggatcggt
     9721 tgtccagggg gaatccaaac caggttccac cttgattcag agaataagta tgacgtattc
     9781 ttggcaatgt ttaagcttga gatgagccga tgtcaaactg ggagacatga tgaaggagga
     9841 aatgttgctt tggtggggga gacactataa ataccaaaga gtgaatggtt gataccattt
     9901 ggggaaggag tgtctatcgc agtctctgca gacggcatat cggatagcct gcaggcgctt
     9961 gtggaggatg cacatgtgac cgagggacac gaagtgatcc gtttaaacta tcagtgtttg
    10021 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat
    10081 ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgccagc cgcctttgcg
    10141 acgctcaccg ggctggttgc cctcgccgct gggctggcgg ccgtctatgg ccctgcaaac
    10201 gcgccagaaa cgccgtcgaa gccgtgtgcg agacaccgcg gccgccggcg ttgtggatac
    10261 ctcgcggaaa acttggccct cactgacaga tgaggggcgg acgttgacac ttgaggggcc
    10321 gactcacccg gcgcggcgtt gacagatgag gggcaggctc gatttcggcc ggcgacgtgg
    10381 agctggccag cctcgcaaat cggcgaaaac gcctgatttt acgcgagttt cccacagatg
    10441 atgtggacaa gcctggggat aagtgccctg cggtattgac acttgagggg cgcgactact
    10501 gacagatgag gggcgcgatc cttgacactt gaggggcaga gtgctgacag atgaggggcg
    10561 cacctattga catttgaggg gctgtccaca ggcagaaaat ccagcatttg caagggtttc
    10621 cgcccgtttt tcggccaccg ctaacctgtc ttttaacctg cttttaaacc aatatttata
    10681 aaccttgttt ttaaccaggg ctgcgccctg tgcgcgtgac cgcgcacgcc gaaggggggt
    10741 gccccccctt ctcgaaccct cccggcccgc taacgcgggc ctcccatccc cccaggggct
    10801 gcgcccctcg gccgcgaacg gcctcacccc aaaaatggca gcgctggcca attcccgagt
    10861 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
    10921 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgg ctaaaatgag
    10981 aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg
    11041 aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat
    11101 gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct
    11161 atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg
    11221 gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga
    11281 acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat
    11341 cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta
    11401 cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagagg acactccatt
    11461 taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt
    11521 cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg
    11581 ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt
    11641 ccggtcgctc agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact
    11701 ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta
    11761 gctgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    11821 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    11881 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    11941 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    12001 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    12061 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    12121 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    12181 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    12241 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    12301 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    12361 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    12421 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    12481 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    12541 tttacggttc ctggcagatc ctagatgtgg cgcaacgatg ccggcgacaa gcaggagcgc
    12601 accgacttct tccgcatcaa gtgttttggc tctcaggccg aggcccacgg caagtatttg
    12661 ggcaaggggt cgctggtatt cgtgcagggc aagattcgga ataccaagta cgagaaggac
    12721 ggccagacgg tctacgggac cgacttcatt gccgataagg tggattatct ggacaccaag
    12781 gcaccaggcg ggtcaaatca ggaataaggg cacattgccc cggcgtgagt cggggcaatc
    12841 ccgcaaggag ggtgaatgaa tcggacgttt gaccggaagg catacaggca agaactgatc
    12901 gacgcggggt tttccgccga ggatgccgaa accatcgcaa gccgcaccgt catgcgtgcg
    12961 ccccgcgaaa ccttccagtc cgtcggctcg atggtccagc aagctacggc caagatcgag
    13021 cgcgacagcg tgcaactggc tccccctgcc ctgcccgcgc catcggccgc cgtggagcgt
    13081 tcgcgtcgtc ttgaacagga ggcggcaggt ttggcgaagt cgatgaccat cgacacgcga
    13141 ggaactatga cgaccaagaa gcgaaaaacc gccggcgagg acctggcaaa acaggtcagc
    13201 gaggccaagc aggccgcgtt gctgaaacac acgaagcagc agatcaagga aatgcagctt
    13261 tccttgttcg atattgcgcc gtggccggac acgatgcgag cgatgccaaa cgacacggcc
    13321 cgctctgccc tgttcaccac gcgcaacaag aaaatcccgc gcgaggcgct gcaaaacaag
    13381 gtcattttcc acgtcaacaa ggacgtgaag atcacctaca ccggcgtcga gctgcgggcc
    13441 gacgatgacg aactggtgtg gcagcaggtg ttggagtacg cgaagcgcac ccctatcggc
    13501 gagccgatca ccttcacgtt ctacgagctt tgccaggacc tgggctggtc gatcaatggc
    13561 cggtattaca cgaaggccga ggaatgcctg tcgcgcctac aggcgacggc gatgggcttc
    13621 acgtccgacc gcgttgggca cctggaatcg gtgtcgctgc tgcaccgctt ccgcgtcctg
    13681 gaccgtggca agaaaacgtc ccgttgccag gtcctgatcg acgaggaaat cgtcgtgctg
    13741 tttgctggcg accactacac gaaattcata tgggagaagt accgcaagct gtcgccgacg
    13801 gcccgacgga tgttcgacta tttcagctcg caccgggagc cgtacccgct caagctggaa
    13861 accttccgcc tcatgtgcgg atcggattcc acccgcgtga agaagtggcg cgagcaggtc
    13921 ggcgaagcct gcgaagagtt gcgaggcagc ggcctggtgg aacacgcctg ggtcaatgat
    13981 gacctggtgc attgcaaacg ctagggcctt gtggggtcag ttccggctgg gggttcagca
    14041 gcccctgctc ggatctgttg gaccggacag tagtcatggt tgatgggctg cctgtatcga
    14101 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
    14161 attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa
    14221 tgtactgggg ttgaacactc t
//
