LOCUS       sequence-305990-       14179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, 
            activating DsRed-SKL with promoter 5xQUAS+CP_ura3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14179)
  AUTHORS   Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
  TITLE     Synthetic control devices for gene regulation in Penicillium 
            chrysogenum.
  JOURNAL   Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 
            10.1186/s12934-019-1253-3.
  PUBMED    31739777
REFERENCE   2  (bases 1 to 14179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2019-11-18"; volume: "18(1):203. doi"; pages: " 
            10.1186/s12934-019-1253-3"
COMMENT     Sequence Label: pLM2_28_p40S_QfDBD_VP16AD_cp_ura3_DsRed_5xQUAS
FEATURES             Location/Qualifiers
     source          1..14179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2781..2891
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2949..2970)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3210..3229)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3557..3578
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3621..3641
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4813..4928
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             7449..8123
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(7579..7597)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7629..7649)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8041..8064
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    9957..9981
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      10069..10779
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10800..10904
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10905..11699
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11870..12458
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12359..12378
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12794..13942
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14088..14112
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
       61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
      121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
      181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
      241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
      301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
      361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
      421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
      481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
      541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
      601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
      661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
      721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
      781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
      841 gaattcaagc ttggaggagt tatagacggt ccggcatagg taaggagaga ttagatattt
      901 cgacttcccg attccttagt ctccttagtc tggaaactga gaaaggcctg aactcgttca
      961 atctattagc tctaatcacg tagtctcaac tagaatcctg aacaggcgcc ggaaccagtc
     1021 caaatgcgtg gagtacagca agtccagtca aatcacgatt agaatggcca tatataagca
     1081 aaaaaccctt ggacaatacg tccgatcgac accaaagcaa tctggtacat cacccttctt
     1141 atatacaggg tagccggaat tgtattgact ctatacgaag ccgcaggttt accagcacag
     1201 aggtgcgata gctacacgaa tgtagagtga aggcattaca accgattgct aagtgtcata
     1261 gaggctaagc ttcacggctc aaggagtctg tggaggatac ctgatataag aattgagagt
     1321 tatactccgg aggttatgtt agtacctcaa aaatgtgaag tacaacgaaa ctcgaacttc
     1381 gaatgacaat agcttggctg gctgttagtc aactgaggta caacgtcatc ttgcaattag
     1441 ttatgttctt tgctagatag tgagctgctg taaacttgta gttcttagaa acatgtctga
     1501 agtctagagt cgagacttgt gattggtgta aattagtggg ggtccggagc ggaataacaa
     1561 tcgaggtcag cctcctgggt ctgtcagtaa gctaaccacg aaaggacaag cgtagacgag
     1621 gtcacggggt actggcctag ccggcttcgg ctagcgccca cgaaaatagg gcaacgtcga
     1681 agcgagagac acacacggat tccacgatcg ccgacgacaa gcactcaact ccacgacaac
     1741 ccacgacaac ccttgccgct gcgttcttgg tgaagggctg caggattaga ggtgagtatt
     1801 ttattttctg gctattgtga tctttttccc ccccggtttc cgctggttct atcttgtcga
     1861 gcgagtcggt gcgccttcga tcggtctgtc aaatttccat ccgtccactc gttcacccgg
     1921 tatcgataca agagggaatg gagcattacg acaggggagc agaggcagaa ctctcgatgt
     1981 tgctactttt tttgcttgga acaacttcct tgaaagattc ttttctgaat gcggctggga
     2041 aactgcgaat tggatatcca gcgaaaggtt accgacagac tcacggccat cgataccact
     2101 cagcgggata gaattgaagg cattcggaca ttagatgatc agcttcgctt gtttcgactc
     2161 gtgctaatca attatccctg gacagtcccc cacatagaca gcaaacaatg ccacccaagc
     2221 gcaaaacgct taacgctgcg gctgaggcta acgctcatgc cgacggacac gccgacggaa
     2281 acgccgacgg acacgtggcc aatacggccg cgtcctcgaa taatgcgagg ttcgctgatc
     2341 tcactaacat cgatactccg ggtctgggac ccacaactac gaccctgctc gtggaaccag
     2401 cacgctcaaa gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca gcccgagaga
     2461 aatgcgacgg aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg tcctgcactt
     2521 atcaggcttc gccgaaaaag aggggagttc aaaccggtta tattcgtacg ctggagctcg
     2581 ccctcgcctg gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat aacctcctcg
     2641 tccgtgacgc cggacaagga tcagctctgc tcgttggtaa agattcgccg gctgccgagc
     2701 gactccatgc ccgttgggct actagccgtg tcaataagag cattacccgc ctcctcaggt
     2761 ttgaaagcgg cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg
     2821 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     2881 ttgacatgct ccccgggttc gttatggtga gcaagggcga ggagctgttc accggggtgg
     2941 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     3001 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     3061 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     3121 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3181 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3241 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3301 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3361 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3421 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3481 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3541 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3601 gcatggacga gctgtacaag ccaaagaaga agcggaaggt ctaagcttac ttctttatcg
     3661 gttctctctt acgacttttt gaatggaacg tttccttctt ctcaggcggg cctatctttg
     3721 ggccgaagct cttttccttg tactgtagga cctggttgat aatgattccc aaaaagacat
     3781 ccagcatgtc agttacttgc attcgtcagt ctatacaaaa gcaatggttt agagaaattt
     3841 tgaactttat acatggtttt atttgttgct tcacggccgt accttctgga aatccacggt
     3901 aggagtgtca atttgcgttt ttgataatcc ttccaaggtt cttctcgaag tagttgttct
     3961 ataattgctt cacagctacc atggaacatc ccaagcaccg ctactagaat tcgagctcgg
     4021 agtggatccc ccgggctgca ggaattcgag ctctgtacag tgaccggtga ctctttctgg
     4081 catgcggaga gacggacgga cgcagagaga agggctgagt aataagcgcc actgcgccag
     4141 acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc
     4201 cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg
     4261 agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt
     4321 gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg
     4381 atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc
     4441 gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc
     4501 ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac
     4561 cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc
     4621 tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca
     4681 aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct
     4741 cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt
     4801 tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc
     4861 tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc
     4921 ttgagcagac atcaccatgc ctcaatcctg ggaagaactg gccgctgata agcgcgcccg
     4981 cctcgcaaaa accatccctg atgaatggaa agtccagacg ctgcctgcgg atgacagcgt
     5041 tattgatttc ccaaagaaat cggggatcct ttcagaggcc gaactgaaga tcacagaggc
     5101 ctccgctgca gatcttgtgt ccaagctggc ggccggagag ttgacctcgg tggaagttac
     5161 gctagcattc tgtaaacggg cagcaatcgc ccagcagtta gtagggtccc ctctacctct
     5221 cagggagatg taacaacgcc accttatggg actatcaagc tgacgctggc ttctgtgcag
     5281 acaaactgcg cccacgagtt cttccctgac gccgctctcg cgcaggcaag ggaactcgat
     5341 gaatactacg caaagcacaa gacacccgtt ggtccactcc atggcctccc catctctctc
     5401 aaagaccagc ttcgagtcaa ggtacaccgt tgcccctaag tcgttagatg tccctttttg
     5461 tcagctaaca tatgccacca gggctacgaa acatcaatgg gctacatctc atggctaaac
     5521 aagtacgacg aaggggactc ggttctgaca accatgctcc gcaaagccgg tgccgttttc
     5581 tacgtcaaga cctctgtccc gcagaccctg atggtctgcg agacagtcaa caacatcatc
     5641 gggcgcaccg tcaacccacg caacaagaac tggtcgtgcg gcggcagttc tggtggtgag
     5701 ggtgcgatcg ttgggattcg tggtggcgtc atcggtgtag gaacggatat cggtggctcg
     5761 attcgagtgc cggccgcgtt caacttcctg tacggtctaa ggccgagtca tgggcggctg
     5821 ccgtatgcaa agatggcgaa cagcatggag ggtcaggaga cggtgcacag cgttgtcggg
     5881 ccgattacgc actctgttga gggtgagtcc ttcgcctctt ccttcttttc ctgctctata
     5941 ccaggcctcc actgtcctcc tttcttgctt tttatactat atacgagacg ggcagtcact
     6001 gatgaagtaa tgttagacct ccgcctcttc accaaatccg tcctcggtca ggagccatgg
     6061 aaatacgact ccaaggtcat ccccatgccc tggcgccagt ccgagtcgga cattattgcc
     6121 tccaagatca agaacggcgg gctcaatatc ggctactaca acttcgacgg caatgtcctt
     6181 ccacaccctc ctatcctgcg cggcgtggaa accaccgtcg ccgcactcgc caaagccggt
     6241 cacaccgtga ccccgtggac gccatacaag cacgatttcg gccacgatct catctcccat
     6301 atctacgcgg ctgacggcag cgccgacgta atgcgcgata tcagtgcatc cggcgagccg
     6361 gcgattccaa atatcaaaga cctactgaac ccgaacatca aagctgttaa catgaacgag
     6421 ctctgggaca cgcatctcca gaagtggaat taccagatgg agtaccttga gaaatggcgg
     6481 gaggctgaag aaaaggccgg gaaggaactg gacgccatca tcgcgccgat tacgcctacc
     6541 gctgcggtac ggcatgacca gttccggtac tatgggtatg cctctgtgat caacctgctg
     6601 gatttcacga gcgtggttgt tccggttacc tttgcggata agaacatcga taagaagaat
     6661 gagagtttca aggcggttag tgagcttgat gccctcgtgc aggaagagta tgatccggag
     6721 gcgtaccatg gggcaccggt tgcagtgcag gttatcggac ggagactcag tgaagagagg
     6781 acgttggcga ttgcagagga agtggggaag ttgctgggaa atgtggtgac tccataggct
     6841 tctaataagt gtcagatagc aatttgcaca agaaatcaat accagcaact gtaaataagc
     6901 gctgaagtga ccatgccatg ctacgaaaga gcagaaaaaa acctgccgta gaaccgaaga
     6961 gatatgacac gcttccatct ctcaaaggaa gaatcccttc agggttgcgt ttccagtcta
     7021 gacacgtata acggcacaag tgtctctcac caaatgggtt atatctcaaa tgtgatctaa
     7081 ggatggaaag cccagaatat tggctgggtt gatggctgct tcgagtgcag tctcatgctg
     7141 ccacaggtga ctctggatgg ccccatacca ctcaacccat ggtacgagcg gtacgcttta
     7201 cgaattccca tggggagggg taatcgatta tcctcggata aacacttatc ccagggtaat
     7261 cacttatccc tggataaaca attatcctcg ggtaatcgct tatccccatc agaaggaaga
     7321 acgaaggaag gagcacagac ttagattggt atatatacgc atatgtggtg ttgaagaaac
     7381 atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag
     7441 ataaatcaat ggcctcctcc gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca
     7501 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     7561 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     7621 gggacatcct gtccccccag ttccagtacg gctccaaggt gtacgtgaag caccccgccg
     7681 acatccccga ctacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     7741 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggctcct
     7801 tcatctacaa ggtgaagttc atcggcgtga acttcccctc cgacggcccc gtaatgcaga
     7861 agaaaactat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga
     7921 agggcgagat ccacaaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca
     7981 agtccatcta catggccaag aagcccgtgc agctgcccgg ctactactac gtggactcca
     8041 agctggacat cacctcccac aacgaggact acaccatcgt ggagcagtac gagcgcgccg
     8101 agggccgcca ccacctgttc ctgactcacg gtatggacga attgtacaag tcgaagctgt
     8161 aagcttgtgc ttctaaggta tgagtcgcaa aattgttttt tatttttggt cttgagtcta
     8221 atatgctcgc agctcttgcg ttgtatatgg tcgttgtcgc gtattttctg ttgtattaaa
     8281 agatcaaacg agatcaaggg atggctcgcg ggctgtctct cgcactagga ggaagaatgc
     8341 ctgaaaaagg aactttgatt ttagctgtgg aatagagatg gcttgtttga ggacgcttgt
     8401 cgcttggcgc agggacttga atggcagctt gtggaaaccg aaggcgagaa aagtcgacgg
     8461 atactgtacg tggttctatt gccagtgcgg tggaagcttc cttctcatat agttcaatcc
     8521 ttctttgaat ctgtttgttt catatttgga ctgtttcatt ctctgcttgc gcattctcat
     8581 cttcgagaac gactgcaggg attgttggtt ctgtggagct gatgagcgcg ccttgaccac
     8641 ccttgttctt gttttgctct tttgttctca tttaacccgt ttctcccttc caaccctttg
     8701 accttgcaac attgtctccc agcgcgttgc caaagcgaac ttgatatcag tatagtatga
     8761 ccaagtagtc taccaaaata aattttagta cagtattgct agttatacaa gataaatttt
     8821 gtacaacaat gattatgcag agctggtatt ctgtaaggag taaattcatg caggtacaga
     8881 gcgtccacgt caggcacacg cgctttagtg ctgggtacac attatgctac ctttggggca
     8941 aggtgctatt ctaggtaggg tatgcctagc aatgccatga tcttatgacc tcagtaatct
     9001 gaccttggat ttaagatcgg agttctcaaa ccctgcgcgc tcagagaatt cgcatgcgga
     9061 gactttagac atacctctcg ttgccgacaa atcggcattc cgtttcggtc acggacgtaa
     9121 gagttcaaca aagccgctca cctgtcccgt tcggcgcttg agtttccact tcaaaagcac
     9181 aaccgctccg atatttccat tgccaccgac caacaaaagg ttcatatcgc gcaatagtcg
     9241 tccaactctc actccgcccg cactcgatgg cgaaggatgg aagaatatcc gagcggcatc
     9301 ggataaagag atctggttct tttcgtgaag taacgtaggg aaccgacgga aactgtagag
     9361 ctataggaat ttagtatatc agaagtcaat tgagagaagt gtatagttag taactcactt
     9421 gtgccaacag gtatttccaa ccgttcttgt tcaaatttcg tcaagtcacc atctctatgt
     9481 gcaaaactgg atcatacaac agctcttatt gtagctgcat ctcgctctca gagatcatcc
     9541 tatcttcgtc gtcatccacc aacacaggtg ggagtgctag ttgatccatg ctgaggtcaa
     9601 gggacacaga cggaatgggt gtagaacgcc tcaaaacagg taaggtatca ggatcggttg
     9661 tccaggggga atccaaacca ggttccacct tgattcagag aataagtatg acgtattctt
     9721 ggcaatgttt aagcttgaga tgagccgatg tcaaactggg agacatgatg aaggaggaaa
     9781 tgttgctttg gtgggggaga cactataaat accaaagagt gaatggttga taccatttgg
     9841 ggaaggagtg tctatcgcag tctctgcaga cggcatatcg gatagcctgc aggcgcttgt
     9901 ggaggatgca catgtgaccg agggacacga agtgatccgt ttaaactatc agtgtttgac
     9961 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
    10021 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgccagccg cctttgcgac
    10081 gctcaccggg ctggttgccc tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc
    10141 gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt gtggatacct
    10201 cgcggaaaac ttggccctca ctgacagatg aggggcggac gttgacactt gaggggccga
    10261 ctcacccggc gcggcgttga cagatgaggg gcaggctcga tttcggccgg cgacgtggag
    10321 ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc cacagatgat
    10381 gtggacaagc ctggggataa gtgccctgcg gtattgacac ttgaggggcg cgactactga
    10441 cagatgaggg gcgcgatcct tgacacttga ggggcagagt gctgacagat gaggggcgca
    10501 cctattgaca tttgaggggc tgtccacagg cagaaaatcc agcatttgca agggtttccg
    10561 cccgtttttc ggccaccgct aacctgtctt ttaacctgct tttaaaccaa tatttataaa
    10621 ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc
    10681 ccccccttct cgaaccctcc cggcccgcta acgcgggcct cccatccccc caggggctgc
    10741 gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc gctggccaat tcccgagtgc
    10801 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    10861 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatggct aaaatgagaa
    10921 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
    10981 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
    11041 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
    11101 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
    11161 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
    11221 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
    11281 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
    11341 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaggac actccattta
    11401 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
    11461 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
    11521 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
    11581 ggtcgctcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
    11641 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagc
    11701 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    11761 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    11821 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    11881 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    11941 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    12001 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    12061 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    12121 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    12181 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    12241 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    12301 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    12361 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    12421 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    12481 tacggttcct ggcagatcct agatgtggcg caacgatgcc ggcgacaagc aggagcgcac
    12541 cgacttcttc cgcatcaagt gttttggctc tcaggccgag gcccacggca agtatttggg
    12601 caaggggtcg ctggtattcg tgcagggcaa gattcggaat accaagtacg agaaggacgg
    12661 ccagacggtc tacgggaccg acttcattgc cgataaggtg gattatctgg acaccaaggc
    12721 accaggcggg tcaaatcagg aataagggca cattgccccg gcgtgagtcg gggcaatccc
    12781 gcaaggaggg tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga
    12841 cgcggggttt tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc
    12901 ccgcgaaacc ttccagtccg tcggctcgat ggtccagcaa gctacggcca agatcgagcg
    12961 cgacagcgtg caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc
    13021 gcgtcgtctt gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg
    13081 aactatgacg accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga
    13141 ggccaagcag gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc
    13201 cttgttcgat attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg
    13261 ctctgccctg ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt
    13321 cattttccac gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga
    13381 cgatgacgaa ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga
    13441 gccgatcacc ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg
    13501 gtattacacg aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac
    13561 gtccgaccgc gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga
    13621 ccgtggcaag aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt
    13681 tgctggcgac cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc
    13741 ccgacggatg ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac
    13801 cttccgcctc atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg
    13861 cgaagcctgc gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga
    13921 cctggtgcat tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagcagc
    13981 ccctgctcgg atctgttgga ccggacagta gtcatggttg atgggctgcc tgtatcgagt
    14041 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
    14101 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
    14161 tactggggtt gaacactct
//
