LOCUS       sequence-305992-       14146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, 
            activating DsRed-SKL with promoter CP_pcbC (no QUAS control).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14146)
  AUTHORS   Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
  TITLE     Synthetic control devices for gene regulation in Penicillium 
            chrysogenum.
  JOURNAL   Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 
            10.1186/s12934-019-1253-3.
  PUBMED    31739777
REFERENCE   2  (bases 1 to 14146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2019-11-18"; volume: "18(1):203. doi"; pages: " 
            10.1186/s12934-019-1253-3"
COMMENT     Sequence Label: pLM2_33_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_0xQUAS
FEATURES             Location/Qualifiers
     source          1..14146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2781..2891
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2949..2970)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3210..3229)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3557..3578
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3621..3641
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4810..4925
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             7416..8090
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(7546..7564)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7596..7616)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8008..8031
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    9924..9948
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      10036..10746
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10767..10871
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10872..11666
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11837..12425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12326..12345
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12761..13909
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14055..14079
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
       61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
      121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
      181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
      241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
      301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
      361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
      421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
      481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
      541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
      601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
      661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
      721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
      781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
      841 gaattcaagc ttggaggagt tatagacggt ccggcatagg taaggagaga ttagatattt
      901 cgacttcccg attccttagt ctccttagtc tggaaactga gaaaggcctg aactcgttca
      961 atctattagc tctaatcacg tagtctcaac tagaatcctg aacaggcgcc ggaaccagtc
     1021 caaatgcgtg gagtacagca agtccagtca aatcacgatt agaatggcca tatataagca
     1081 aaaaaccctt ggacaatacg tccgatcgac accaaagcaa tctggtacat cacccttctt
     1141 atatacaggg tagccggaat tgtattgact ctatacgaag ccgcaggttt accagcacag
     1201 aggtgcgata gctacacgaa tgtagagtga aggcattaca accgattgct aagtgtcata
     1261 gaggctaagc ttcacggctc aaggagtctg tggaggatac ctgatataag aattgagagt
     1321 tatactccgg aggttatgtt agtacctcaa aaatgtgaag tacaacgaaa ctcgaacttc
     1381 gaatgacaat agcttggctg gctgttagtc aactgaggta caacgtcatc ttgcaattag
     1441 ttatgttctt tgctagatag tgagctgctg taaacttgta gttcttagaa acatgtctga
     1501 agtctagagt cgagacttgt gattggtgta aattagtggg ggtccggagc ggaataacaa
     1561 tcgaggtcag cctcctgggt ctgtcagtaa gctaaccacg aaaggacaag cgtagacgag
     1621 gtcacggggt actggcctag ccggcttcgg ctagcgccca cgaaaatagg gcaacgtcga
     1681 agcgagagac acacacggat tccacgatcg ccgacgacaa gcactcaact ccacgacaac
     1741 ccacgacaac ccttgccgct gcgttcttgg tgaagggctg caggattaga ggtgagtatt
     1801 ttattttctg gctattgtga tctttttccc ccccggtttc cgctggttct atcttgtcga
     1861 gcgagtcggt gcgccttcga tcggtctgtc aaatttccat ccgtccactc gttcacccgg
     1921 tatcgataca agagggaatg gagcattacg acaggggagc agaggcagaa ctctcgatgt
     1981 tgctactttt tttgcttgga acaacttcct tgaaagattc ttttctgaat gcggctggga
     2041 aactgcgaat tggatatcca gcgaaaggtt accgacagac tcacggccat cgataccact
     2101 cagcgggata gaattgaagg cattcggaca ttagatgatc agcttcgctt gtttcgactc
     2161 gtgctaatca attatccctg gacagtcccc cacatagaca gcaaacaatg ccacccaagc
     2221 gcaaaacgct taacgctgcg gctgaggcta acgctcatgc cgacggacac gccgacggaa
     2281 acgccgacgg acacgtggcc aatacggccg cgtcctcgaa taatgcgagg ttcgctgatc
     2341 tcactaacat cgatactccg ggtctgggac ccacaactac gaccctgctc gtggaaccag
     2401 cacgctcaaa gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca gcccgagaga
     2461 aatgcgacgg aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg tcctgcactt
     2521 atcaggcttc gccgaaaaag aggggagttc aaaccggtta tattcgtacg ctggagctcg
     2581 ccctcgcctg gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat aacctcctcg
     2641 tccgtgacgc cggacaagga tcagctctgc tcgttggtaa agattcgccg gctgccgagc
     2701 gactccatgc ccgttgggct actagccgtg tcaataagag cattacccgc ctcctcaggt
     2761 ttgaaagcgg cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg
     2821 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     2881 ttgacatgct ccccgggttc gttatggtga gcaagggcga ggagctgttc accggggtgg
     2941 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     3001 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     3061 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     3121 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3181 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3241 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3301 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3361 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3421 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3481 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3541 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3601 gcatggacga gctgtacaag ccaaagaaga agcggaaggt ctaagcttac ttctttatcg
     3661 gttctctctt acgacttttt gaatggaacg tttccttctt ctcaggcggg cctatctttg
     3721 ggccgaagct cttttccttg tactgtagga cctggttgat aatgattccc aaaaagacat
     3781 ccagcatgtc agttacttgc attcgtcagt ctatacaaaa gcaatggttt agagaaattt
     3841 tgaactttat acatggtttt atttgttgct tcacggccgt accttctgga aatccacggt
     3901 aggagtgtca atttgcgttt ttgataatcc ttccaaggtt cttctcgaag tagttgttct
     3961 ataattgctt cacagctacc atggaacatc ccaagcaccg ctactagaat tcgagctcgg
     4021 agtggatccc ccgggctgca ggaattcgag ctctgtacag tgaccggtga ctctttctgg
     4081 catgcggaga gacggacgga cgcagagaga agggctgagt aataagccac tggccagaca
     4141 gctctggcgg ctctgaggtg cagtggatga ttattaatcc gggaccggcc gcccctccgc
     4201 cccgaagtgg aaaggctggt gtgcccctcg ttgaccaaga atctattgca tcatcggaga
     4261 atatggagct tcatcgaatc accggcagta agcgaaggag aatgtgaagc caggggtgta
     4321 tagccgtcgg cgaaatagca tgccattaac ctaggtacag aagtccaatt gcttccgatc
     4381 tggtaaaaga ttcacgagat agtaccttct ccgaagtagg tagagcgagt acccggcgcg
     4441 taagctccct aattggccca tccggcatct gtagggcgtc caaatatcgt gcctctcctg
     4501 ctttgcccgg tgtatgaaac cggaaaggcc gctcaggagc tggccagcgg cgcagaccgg
     4561 gaacacaagc tggcagtcga cccatccggt gctctgcact cgacctgctg aggtccctca
     4621 gtccctggta ggcagctttg ccccgtctgt ccgcccggtg tgtcggcggg gttgacaagg
     4681 tcgttgcgtc agtccaacat ttgttgccat attttcctgc tctccccacc agctgctctt
     4741 ttcttttctc tttcttttcc catcttcagt atattcatct tcccatccaa gaacctttat
     4801 ttcccctaag taagtacttt gctacatcca tactccatcc ttcccatccc ttattccttt
     4861 gaacctttca gttcgagctt tcccacttca tcgcagcttg actaacagct accccgcttg
     4921 agcagacatc acaatgcctc aatcctggga agaactggcc gctgataagc gcgcccgcct
     4981 cgcaaaaacc atccctgatg aatggaaagt ccagacgctg cctgcggatg acagcgttat
     5041 tgatttccca aagaaatcgg ggatcctttc agaggccgaa ctgaagatca cagaggcctc
     5101 cgctgcagat cttgtgtcca agctggcggc cggagagttg acctcggtgg aagttacgct
     5161 agcattctgt aaacgggcag caatcgccca gcagttagta gggtcccctc tacctctcag
     5221 ggagatgtaa caacgccacc ttatgggact atcaagctga cgctggcttc tgtgcagaca
     5281 aactgcgccc acgagttctt ccctgacgcc gctctcgcgc aggcaaggga actcgatgaa
     5341 tactacgcaa agcacaagac acccgttggt ccactccatg gcctccccat ctctctcaaa
     5401 gaccagcttc gagtcaaggt acaccgttgc ccctaagtcg ttagatgtcc ctttttgtca
     5461 gctaacatat gccaccaggg ctacgaaaca tcaatgggct acatctcatg gctaaacaag
     5521 tacgacgaag gggactcggt tctgacaacc atgctccgca aagccggtgc cgttttctac
     5581 gtcaagacct ctgtcccgca gaccctgatg gtctgcgaga cagtcaacaa catcatcggg
     5641 cgcaccgtca acccacgcaa caagaactgg tcgtgcggcg gcagttctgg tggtgagggt
     5701 gcgatcgttg ggattcgtgg tggcgtcatc ggtgtaggaa cggatatcgg tggctcgatt
     5761 cgagtgccgg ccgcgttcaa cttcctgtac ggtctaaggc cgagtcatgg gcggctgccg
     5821 tatgcaaaga tggcgaacag catggagggt caggagacgg tgcacagcgt tgtcgggccg
     5881 attacgcact ctgttgaggg tgagtccttc gcctcttcct tcttttcctg ctctatacca
     5941 ggcctccact gtcctccttt cttgcttttt atactatata cgagacgggc agtcactgat
     6001 gaagtaatgt tagacctccg cctcttcacc aaatccgtcc tcggtcagga gccatggaaa
     6061 tacgactcca aggtcatccc catgccctgg cgccagtccg agtcggacat tattgcctcc
     6121 aagatcaaga acggcgggct caatatcggc tactacaact tcgacggcaa tgtccttcca
     6181 caccctccta tcctgcgcgg cgtggaaacc accgtcgccg cactcgccaa agccggtcat
     6241 accgtgaccc cgtggacgcc atacaagcac gatttcggcc acgatctcat ctcccatatc
     6301 tacgcggctg acggcagcgc cgacgtaatg cgcgatatca gtgcatccgg cgagccggcg
     6361 attccaaata tcaaagacct actgaacccg aacatcaaag ctgttaacat gaacgagctc
     6421 tgggacacgc atctccagaa gtggaattac cagatggagt accttgagaa atggcgggag
     6481 gctgaagaaa aggccgggaa ggaactggac gccatcatcg cgccgattac gcctaccgct
     6541 gcggtacggc atgaccagtt ccggtactat gggtatgcct ctgtgatcaa cctgctggat
     6601 ttcacgagcg tggttgttcc ggttaccttt gcggataaga acatcgataa gaagaatgag
     6661 agtttcaagg cggttagtga gcttgatgcc ctcgtgcagg aagagtatga tccggaggcg
     6721 taccatgggg caccggttgc agtgcaggtt atcggacgga gactcagtga agagaggacg
     6781 ttggcgattg cagaggaagt ggggaagttg ctgggaaatg tggtgactcc ataggcttct
     6841 aataagtgtc agatagcaat ttgcacaaga aatcaatacc agcaactgta aataagcgct
     6901 gaagtgacca tgccatgcta cgaaagagca gaaaaaaacc tgccgtagaa ccgaagagat
     6961 atgacacgct tccatctctc aaaggaagaa tcccttcagg gttgcgtttc cagtctagac
     7021 acgtataacg gcacaagtgt ctctcaccaa atgggttata tctcaaatgt gatctaagga
     7081 tggaaagccc agaatattgg ctgggttgat ggctgcttcg agtgcagtct catgctgcca
     7141 caggtgactc tggatggccc cataccactc aacccatggt acgagcggta cgctttacga
     7201 attcccatgg ggagcgtata atgtctccag gttgtctcag cataaacacc ccgcccccgc
     7261 tcaggcacac aggaagagag ctcaggtcgt ttccattgcg tccatactct tcactcattg
     7321 tcatctgcag gagaacttcc cctgtccctt tgccaagccc tctcttcgtc gttgtccacg
     7381 ccttcaagtt ttcaccatta tttttctaga caccaatggc ctcctccgag gacgtcatca
     7441 aggagttcat gcgcttcaag gtgcgcatgg agggctccgt gaacggccac gagttcgaga
     7501 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     7561 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct
     7621 ccaaggtgta cgtgaagcac cccgccgaca tccccgacta caagaagctg tccttccccg
     7681 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     7741 aggactcctc cctgcaggac ggctccttca tctacaaggt gaagttcatc ggcgtgaact
     7801 tcccctccga cggccccgta atgcagaaga aaactatggg ctgggaggcc tccaccgagc
     7861 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca caaggccctg aagctgaagg
     7921 acggcggcca ctacctggtg gagttcaagt ccatctacat ggccaagaag cccgtgcagc
     7981 tgcccggcta ctactacgtg gactccaagc tggacatcac ctcccacaac gaggactaca
     8041 ccatcgtgga gcagtacgag cgcgccgagg gccgccacca cctgttcctg actcacggta
     8101 tggacgaatt gtacaagtcg aagctgtaag cttgtgcttc taaggtatga gtcgcaaaat
     8161 tgttttttat ttttggtctt gagtctaata tgctcgcagc tcttgcgttg tatatggtcg
     8221 ttgtcgcgta ttttctgttg tattaaaaga tcaaacgaga tcaagggatg gctcgcgggc
     8281 tgtctctcgc actaggagga agaatgcctg aaaaaggaac tttgatttta gctgtggaat
     8341 agagatggct tgtttgagga cgcttgtcgc ttggcgcagg gacttgaatg gcagcttgtg
     8401 gaaaccgaag gcgagaaaag tcgacggata ctgtacgtgg ttctattgcc agtgcggtgg
     8461 aagcttcctt ctcatatagt tcaatccttc tttgaatctg tttgtttcat atttggactg
     8521 tttcattctc tgcttgcgca ttctcatctt cgagaacgac tgcagggatt gttggttctg
     8581 tggagctgat gagcgcgcct tgaccaccct tgttcttgtt ttgctctttt gttctcattt
     8641 aacccgtttc tcccttccaa ccctttgacc ttgcaacatt gtctcccagc gcgttgccaa
     8701 agcgaacttg atatcagtat agtatgacca agtagtctac caaaataaat tttagtacag
     8761 tattgctagt tatacaagat aaattttgta caacaatgat tatgcagagc tggtattctg
     8821 taaggagtaa attcatgcag gtacagagcg tccacgtcag gcacacgcgc tttagtgctg
     8881 ggtacacatt atgctacctt tggggcaagg tgctattcta ggtagggtat gcctagcaat
     8941 gccatgatct tatgacctca gtaatctgac cttggattta agatcggagt tctcaaaccc
     9001 tgcgcgctca gagaattcgc atgcggagac tttagacata cctctcgttg ccgacaaatc
     9061 ggcattccgt ttcggtcacg gacgtaagag ttcaacaaag ccgctcacct gtcccgttcg
     9121 gcgcttgagt ttccacttca aaagcacaac cgctccgata tttccattgc caccgaccaa
     9181 caaaaggttc atatcgcgca atagtcgtcc aactctcact ccgcccgcac tcgatggcga
     9241 aggatggaag aatatccgag cggcatcgga taaagagatc tggttctttt cgtgaagtaa
     9301 cgtagggaac cgacggaaac tgtagagcta taggaattta gtatatcaga agtcaattga
     9361 gagaagtgta tagttagtaa ctcacttgtg ccaacaggta tttccaaccg ttcttgttca
     9421 aatttcgtca agtcaccatc tctatgtgca aaactggatc atacaacagc tcttattgta
     9481 gctgcatctc gctctcagag atcatcctat cttcgtcgtc atccaccaac acaggtggga
     9541 gtgctagttg atccatgctg aggtcaaggg acacagacgg aatgggtgta gaacgcctca
     9601 aaacaggtaa ggtatcagga tcggttgtcc agggggaatc caaaccaggt tccaccttga
     9661 ttcagagaat aagtatgacg tattcttggc aatgtttaag cttgagatga gccgatgtca
     9721 aactgggaga catgatgaag gaggaaatgt tgctttggtg ggggagacac tataaatacc
     9781 aaagagtgaa tggttgatac catttgggga aggagtgtct atcgcagtct ctgcagacgg
     9841 catatcggat agcctgcagg cgcttgtgga ggatgcacat gtgaccgagg gacacgaagt
     9901 gatccgttta aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag
     9961 agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca
    10021 tttgtatgtg ccagccgcct ttgcgacgct caccgggctg gttgccctcg ccgctgggct
    10081 ggcggccgtc tatggccctg caaacgcgcc agaaacgccg tcgaagccgt gtgcgagaca
    10141 ccgcggccgc cggcgttgtg gatacctcgc ggaaaacttg gccctcactg acagatgagg
    10201 ggcggacgtt gacacttgag gggccgactc acccggcgcg gcgttgacag atgaggggca
    10261 ggctcgattt cggccggcga cgtggagctg gccagcctcg caaatcggcg aaaacgcctg
    10321 attttacgcg agtttcccac agatgatgtg gacaagcctg gggataagtg ccctgcggta
    10381 ttgacacttg aggggcgcga ctactgacag atgaggggcg cgatccttga cacttgaggg
    10441 gcagagtgct gacagatgag gggcgcacct attgacattt gaggggctgt ccacaggcag
    10501 aaaatccagc atttgcaagg gtttccgccc gtttttcggc caccgctaac ctgtctttta
    10561 acctgctttt aaaccaatat ttataaacct tgtttttaac cagggctgcg ccctgtgcgc
    10621 gtgaccgcgc acgccgaagg ggggtgcccc cccttctcga accctcccgg cccgctaacg
    10681 cgggcctccc atccccccag gggctgcgcc cctcggccgc gaacggcctc accccaaaaa
    10741 tggcagcgct ggccaattcc cgagtgcgcg gaacccctat ttgtttattt ttctaaatac
    10801 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    10861 aaaggaagag tatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat
    10921 accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag
    10981 aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg
    11041 tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc
    11101 tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc
    11161 tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg
    11221 agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag
    11281 acagccgctt agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg
    11341 aaaactggga agaggacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga
    11401 cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct
    11461 ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca
    11521 agtggtatga cattgccttc tgcgtccggt cgctcaggga ggatatcggg gaagaacagt
    11581 atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt
    11641 atattttact ggatgaattg ttttagctgt cagaccaagt ttactcatat atactttaga
    11701 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    11761 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    11821 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    11881 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    11941 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
    12001 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    12061 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    12121 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    12181 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    12241 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    12301 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    12361 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    12421 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc agatcctaga tgtggcgcaa
    12481 cgatgccggc gacaagcagg agcgcaccga cttcttccgc atcaagtgtt ttggctctca
    12541 ggccgaggcc cacggcaagt atttgggcaa ggggtcgctg gtattcgtgc agggcaagat
    12601 tcggaatacc aagtacgaga aggacggcca gacggtctac gggaccgact tcattgccga
    12661 taaggtggat tatctggaca ccaaggcacc aggcgggtca aatcaggaat aagggcacat
    12721 tgccccggcg tgagtcgggg caatcccgca aggagggtga atgaatcgga cgtttgaccg
    12781 gaaggcatac aggcaagaac tgatcgacgc ggggttttcc gccgaggatg ccgaaaccat
    12841 cgcaagccgc accgtcatgc gtgcgccccg cgaaaccttc cagtccgtcg gctcgatggt
    12901 ccagcaagct acggccaaga tcgagcgcga cagcgtgcaa ctggctcccc ctgccctgcc
    12961 cgcgccatcg gccgccgtgg agcgttcgcg tcgtcttgaa caggaggcgg caggtttggc
    13021 gaagtcgatg accatcgaca cgcgaggaac tatgacgacc aagaagcgaa aaaccgccgg
    13081 cgaggacctg gcaaaacagg tcagcgaggc caagcaggcc gcgttgctga aacacacgaa
    13141 gcagcagatc aaggaaatgc agctttcctt gttcgatatt gcgccgtggc cggacacgat
    13201 gcgagcgatg ccaaacgaca cggcccgctc tgccctgttc accacgcgca acaagaaaat
    13261 cccgcgcgag gcgctgcaaa acaaggtcat tttccacgtc aacaaggacg tgaagatcac
    13321 ctacaccggc gtcgagctgc gggccgacga tgacgaactg gtgtggcagc aggtgttgga
    13381 gtacgcgaag cgcaccccta tcggcgagcc gatcaccttc acgttctacg agctttgcca
    13441 ggacctgggc tggtcgatca atggccggta ttacacgaag gccgaggaat gcctgtcgcg
    13501 cctacaggcg acggcgatgg gcttcacgtc cgaccgcgtt gggcacctgg aatcggtgtc
    13561 gctgctgcac cgcttccgcg tcctggaccg tggcaagaaa acgtcccgtt gccaggtcct
    13621 gatcgacgag gaaatcgtcg tgctgtttgc tggcgaccac tacacgaaat tcatatggga
    13681 gaagtaccgc aagctgtcgc cgacggcccg acggatgttc gactatttca gctcgcaccg
    13741 ggagccgtac ccgctcaagc tggaaacctt ccgcctcatg tgcggatcgg attccacccg
    13801 cgtgaagaag tggcgcgagc aggtcggcga agcctgcgaa gagttgcgag gcagcggcct
    13861 ggtggaacac gcctgggtca atgatgacct ggtgcattgc aaacgctagg gccttgtggg
    13921 gtcagttccg gctgggggtt cagcagcccc tgctcggatc tgttggaccg gacagtagtc
    13981 atggttgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
    14041 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
    14101 aataacacat tgcggacgtt tttaatgtac tggggttgaa cactct
//
