LOCUS       sequence-305997-       11320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon vector with CAG promoter expressing 
            SMARCA4-Venus fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   Ballabio C, Anderle M, Gianesello M, Lago C, Miele E, Cardano M, 
            Aiello G, Piazza S, Caron D, Gianno F, Ciolfi A, Pedace L, 
            Mastronuzzi A, Tartaglia M, Locatelli F, Ferretti E, Giangaspero F, 
            Tiberi L
  TITLE     Modeling medulloblastoma in vivo and with human cerebellar 
            organoids.
  JOURNAL   Nat Commun. 2020 Jan 29;11(1):583. doi: 10.1038/s41467-019-13989-3.
  PUBMED    31996670
REFERENCE   2  (bases 1 to 11320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-29"; volume: "11(1):583. doi"; pages: " 
            10.1038/s41467-019-13989-3"
COMMENT     Sequence Label: pPB CAG SMARCA4-Venus
FEATURES             Location/Qualifiers
     source          1..11320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   38..72
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        355..734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        736..1013
                     /label=chicken beta-actin promoter
     intron          1014..2030
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1954..1976
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2038..2057
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(6967..6969,6970..7683)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7009..7030)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7270..7289)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7617..7638
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7777..7796)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7842..7897
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7896..7915)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   8425..8487
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(8511..8529)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8550..8566)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8550..8566)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8563..8585)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8574..8590
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8598..8604,8605..8622,8623..8628))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8643..8664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8781..8798)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8952..9539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9031..9050)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9710..10501,10502..10570))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10333..10352
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10571..10675)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10701..11156)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10838..10859)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11050..11069
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11283..11305
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11297..11314
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11298..11314
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgggt accttaaccc tagaaagata gtctgcgtaa
       61 aattgacgca tgcattcttg aaatattgct ctctctttct aaatagcgcg aatccgtcgc
      121 tgtgcattta ggacatctca gtcgccgctt ggagctcccg tgaggcgtgc ttgtcaatgc
      181 ggtaagtgtc actgattttg aactataacg accgcgtgag tcaaaatgac gcatgattat
      241 cttttacgtg acttttaaga tttaactcat acgataatta tattgttatt tcatgttcta
      301 cttacgtgat aacttattat atatatattt tcttgttata gatatcgtcg ggtcgacatt
      361 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      421 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      481 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      541 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      601 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      661 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      721 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
      781 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
      841 cggggggggg gggggggcgc gcgccrggsg gggsggggsg gggggrgggg sggggsgggg
      901 sgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
      961 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     1021 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     1081 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     1141 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
     1201 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
     1261 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
     1321 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     1381 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     1441 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     1501 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     1561 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     1621 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc
     1681 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     1741 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     1801 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     1861 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     1921 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1981 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     2041 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgcatgtc cactccagac
     2101 ccacccctgg gcggaactcc tcggccaggt ccttccccgg gccctggccc ttcccctgga
     2161 gccatgctgg gccctagccc gggtccctcg ccgggctccg cccacagcat gatggggccc
     2221 agcccagggc cgccctcagc aggacacccc atccccaccc aggggcctgg agggtaccct
     2281 caggacaaca tgcaccagat gcacaagccc atggagtcca tgcatgagaa gggcatgtcg
     2341 gacgacccgc gctacaacca gatgaaagga atggggatgc ggtcaggggg ccatgctggg
     2401 atggggcccc cgcccagccc catggaccag cactcccaag gttacccctc gcccctgggt
     2461 ggctctgagc atgcctctag tccagttcca gccagtggcc cgtcttcggg gccccagatg
     2521 tcttccgggc caggaggtgc cccgctggat ggtgctgacc cccaggcctt ggggcagcag
     2581 aaccggggcc caaccccatt taaccagaac cagctgcacc agctcagagc tcagatcatg
     2641 gcctacaaga tgctggccag ggggcagccc ctccccgacc acctgcagat ggcggtgcag
     2701 ggcaagcggc cgatgcccgg gatgcagcag cagatgccaa cgctacctcc accctcggtg
     2761 tccgcaacag gacccggccc tggccctggc cctggccccg gcccgggtcc cggcccggca
     2821 cctccaaatt acagcaggcc tcatggtatg ggagggccca acatgcctcc cccaggaccc
     2881 tcgggcgtgc cccccgggat gccaggccag cctcctggag ggcctcccaa gccctggcct
     2941 gaaggaccca tggcgaatgc tgctgccccc acgagcaccc ctcagaagct gattcccccg
     3001 cagccaacgg gccgcccttc ccccgcgccc cctgccgtcc cacccgccgc ctcgcccgtg
     3061 atgccaccgc agacccagtc ccccgggcag ccggcccagc ccgcgcccat ggtgccactg
     3121 caccagaagc agagccgcat cacccccatc cagaagccgc ggggcctcga ccctgtggag
     3181 atcctgcagg agcgcgagta caggctgcag gctcgcatcg cacaccgaat tcaggaactt
     3241 gaaaaccttc ccgggtccct ggccggggat ttgcgaacca aagcgaccat tgagctcaag
     3301 gccctcaggc tgctgaactt ccagaggcag ctgcgccagg aggtggtggt gtgcatgcgg
     3361 agggacacag cgctggagac agccctcaat gctaaggcct acaagcgcag caagcgccag
     3421 tccctgcgcg aggcccgcat cactgagaag ctggagaagc agcagaagat cgagcaggag
     3481 cgcaagcgcc ggcagaagca ccaggaatac ctcaatagca ttctccagca tgccaaggat
     3541 ttcaaggaat atcacagatc cgtcacaggc aaaatccaga agctgaccaa ggcagtggcc
     3601 acgtaccatg ccaacacgga gcgggagcag aagaaagaga acgagcggat cgagaaggag
     3661 cgcatgcgga ggctcatggc tgaagatgag gaggggtacc gcaagctcat cgaccagaag
     3721 aaggacaagc gcctggccta cctcttgcag cagacagacg agtacgtggc taacctcacg
     3781 gagctggtgc ggcagcacaa ggctgcccag gtcgccaagg agaaaaagaa gaaaaagaaa
     3841 aagaagaagg cagaaaatgc agaaggacag acgcctgcca ttgggccgga tggcgagcct
     3901 ctggacgaga ccagccagat gagcgacctc ccggtgaagg tgatccacgt ggagagtggg
     3961 aagatcctca caggcacaga tgcccccaaa gccgggcagc tggaggcctg gctcgagatg
     4021 aacccggggt atgaagtagc tccgaggtct gatagtgaag aaagtggctc agaagaagag
     4081 gaagaggagg aggaggaaga gcagccgcag gcagcacagc ctcccaccct gcccgtggag
     4141 gagaagaaga agattccaga tccagacagc gatgacgtct ctgaggtgga cgcgcggcac
     4201 atcattgaga atgccaagca agatgtcgat gatgaatatg gcgtgtccca ggcccttgca
     4261 cgtggcctgc agtcctacta tgccgtggcc catgctgtca ctgagagagt ggacaagcag
     4321 tcagcgctta tggtcaatgg tgtcctcaaa cagtaccaga tcaaaggttt ggagtggctg
     4381 gtgtccctgt acaacaacaa cctgaacggc atcctggccg acgagatggg cctggggaag
     4441 accatccaga ccatcgcgct catcacgtac ctcatggagc acaaacgcat caatgggccc
     4501 ttcctcatca tcgtgcctct ctcaacgctg tccaactggg cgtacgagtt tgacaagtgg
     4561 gccccctccg tggtgaaggt gtcttacaag ggatccccag cagcaagacg ggcctttgtc
     4621 ccccagctcc ggagtgggaa gttcaacgtc ttgctgacga cgtacgagta catcatcaaa
     4681 gacaagcaca tcctcgccaa gatccgttgg aagtacatga ttgtggacga aggtcaccgc
     4741 atgaagaacc accactgcaa gctgacgcag gtgctcaaca cgcactatgt ggcaccccgc
     4801 cgcctgctgc tgacgggcac accgctgcag aacaagcttc ccgagctctg ggcgctgctc
     4861 aacttcctgc tgcccaccat cttcaagagc tgcagcacct tcgagcagtg gtttaacgca
     4921 ccctttgcca tgaccgggga aaaggtggac ctgaatgagg aggaaaccat tctcatcatc
     4981 cggcgtctcc acaaagtgct gcggcccttc ttgctccgac gactcaagaa ggaagtcgag
     5041 gcccagttgc ccgaaaaggt ggagtacgtc atcaagtgcg acatgtctgc gctgcagcga
     5101 gtgctctacc gccacatgca ggccaagggc gtgctgctga ctgatggctc cgagaaggac
     5161 aagaagggca aaggcggcac caagaccctg atgaacacca tcatgcagct gcggaagatc
     5221 tgcaaccacc cctacatgtt ccagcacatc gaggagtcct tttccgagca cttggggttc
     5281 actggcggca ttgtccaagg gctggacctg taccgagcct cgggtaaatt tgagcttctt
     5341 gatagaattc ttcccaaact ccgagcaacc aaccacaaag tgctgctgtt ctgccaaatg
     5401 acctccctca tgaccatcat ggaagattac tttgcgtatc gcggctttaa atacctcagg
     5461 cttgatggaa ccacgaaggc ggaggaccgg ggcatgctgc tgaaaacctt caacgagccc
     5521 ggctctgagt acttcatctt cctgctcagc acccgggctg gggggctcgg cctgaacctc
     5581 cagtcggcag acactgtgat catttttgac agcgactgga atcctcacca ggacctgcaa
     5641 gcgcaggacc gagcccaccg catcgggcag cagaacgagg tgcgtgtgct ccgcctctgc
     5701 accgtcaaca gcgtggagga gaagatccta gctgcagcca agtacaagct caacgtggac
     5761 cagaaggtga tccaggccgg catgttcgac cagaagtcct caagccatga gcggcgcgcc
     5821 ttcctgcagg ccatcctgga gcacgaggag caggatgagg aggaagacaa ggtgcccgac
     5881 gacgagaccg tcaaccagat gatcgcccgg cacgaggagg agtttgatct gttcatgcgc
     5941 atggacctgg accgcaggcg cgaggaggcc cgcaacccca agcggaagcc gcgcctcatg
     6001 gaggaggacg agctcccctc gtggatcatc aaggacgacg cggaggtgga gcggctgacc
     6061 tgtgaggagg aggaggagaa gatgttcggc cgtggctccc gccaccgcaa ggaggtggac
     6121 tacagcgact cactgacgga gaagcagtgg ctcaaggcca tcgaggaggg cacgctggag
     6181 gagatcgaag aggaggtccg gcagaagaaa tcatcacgga agcgcaagcg agacagcgac
     6241 gccggctcct ccaccccgac caccagcacc cgcagccgcg acaaggacga cgagagcaag
     6301 aagcagaaga agcgcgggcg gccgcctgcc gagaaactct cccctaaccc acccaacctc
     6361 accaagaaga tgaagaagat tgtggatgcc gtgatcaagt acaaggacag cagcagtgga
     6421 cgtcagctca gcgaggtctt catccagctg ccctcgcgaa aggagctgcc cgagtactac
     6481 gagctcatcc gcaagcccgt ggacttcaag aagataaagg agcgcattcg caaccacaag
     6541 taccgcagcc tcaacgacct agagaaggac gtcatgctcc tgtgccagaa cgcacagacc
     6601 ttcaacctgg agggctccct gatctatgaa gactccatcg tcttgcagtc ggtcttcacc
     6661 agcgtgcggc agaaaatcga gaaggaggat gacagtgaag gcgaggagag tgaggaggag
     6721 gaagagggcg aggaggaagg ctccgaatcc gaatctcggt ccgtcaaagt gaagatcaag
     6781 cttggccgga aggagaaggc acaggaccag ctgaagggcg gccggcggcg gccgagccga
     6841 gggtcccgag ccaagccggt cgtgagtgac gatgacagtg aggaggaaca agaggaggac
     6901 cgctcaggaa gtggcagcga agaagacacc ggtggttctg gtggtggttc tggtggtggt
     6961 tctggtgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     7021 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     7081 tacggcaagc tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc
     7141 accctcgtga ccaccctggg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg
     7201 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     7261 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     7321 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     7381 cacaagctgg agtacaacta caacagccac aacgtctata tcaccgccga caagcagaag
     7441 aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc
     7501 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     7561 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     7621 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     7681 tgatgagcgg ccgcaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     7741 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     7801 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     7861 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     7921 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     7981 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     8041 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     8101 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     8161 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
     8221 atccctcgac ctgcaccgtc tatggagatc ggatcctttg ttactttata gaagaaattt
     8281 tgagtttttg ttttttttta ataaataaat aaacataaat aaattgtttg ttgaatttat
     8341 tattagtatg taagtgtaaa tataataaaa cttaatatct attcaaatta ataaataaac
     8401 ctcgatatac agaccgataa aacacatgcg tcaattttac gcatgattat ctttaacgta
     8461 cgtcacaata tgattatctt tctagggtta aagagctcca gcttttgttc cctttagtga
     8521 gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8581 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8641 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8701 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8761 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8821 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8881 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8941 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9001 agtcagaggt ggcgaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     9061 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     9121 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     9181 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     9241 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9301 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9361 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9421 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9481 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9541 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9601 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9661 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9721 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9781 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9841 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9901 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9961 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    10021 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    10081 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    10141 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    10201 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    10261 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10321 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10381 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10441 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10501 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10561 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10621 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10681 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
    10741 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
    10801 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
    10861 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
    10921 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
    10981 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
    11041 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
    11101 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
    11161 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    11221 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    11281 ttcccagtca cgacgttgta aaacgacggc cagtgaattg
//
