LOCUS       sequence-306033-        6506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dimerization dependent fluorescent protein GB core domain fused to 
            mRNA decapping enzyme 1b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Lee JE, Cathey PI, Wu H, Parker R, Voeltz GK
  TITLE     Endoplasmic reticulum contact sites regulate the dynamics of 
            membraneless organelles.
  JOURNAL   Science. 2020 Jan 31;367(6477). pii: 367/6477/eaay7108. doi: 
            10.1126/science.aay7108.
  PUBMED    32001628
REFERENCE   2  (bases 1 to 6506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2020-01-31"; volume: "367(6477). pii: 367/6477/eaay7108. 
            doi"; pages: " 10.1126/science.aay7108"
COMMENT     Sequence Label: GB-Dcp1b
FEATURES             Location/Qualifiers
     source          1..6506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(655..673)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     923..942
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1117..1140
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    3234..3355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3271..3290)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3325..3344
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3362..3817)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3499..3520)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3711..3730
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3844..3948
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3950..4307
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3950..4141
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3973..3993)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4158..4293
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4342..5136
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4342..5136
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4396..4415)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5006..5025
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5324..5343)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5368..5415
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5744..6332
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6233..6252
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgg
      541 ccaccatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg
      601 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      661 tgaaggtgac caagggcggc cccctgccct ttgcctggga catcctgtcc ccccagatca
      721 tgtacggctc caaagcgtac gtgaggcacc ccgccgacat ccccgattac aagaagctgc
      781 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaagacggc ggtctggtga
      841 ccgttaccca ggactcctcc ctgcaggacg gcacgctgat ctgcaaggtg aagattcgcg
      901 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
      961 ccaccgagat gctgtacccc aaggacggcg tgctgaaggg ccatagctat caggccctga
     1021 agctgaagga cggcggccac tacctggtgg agttcgagac catctacatg gccaagaagc
     1081 ccgtgcaact gcccggcgat tactgtgtgg acaccaagct ggacatcacc tcccacaacg
     1141 aggactacac catcgtggaa cagtacgggc gctccgaggg ccgccaccgt ctgggcatgg
     1201 acgagcggta caagtccgga ctcagatctc gagctgcagc cgtggcggca ggcggcctgg
     1261 tgggaaaggg gcgcgacatc agcctagcgg ccctgcagcg ccacgacccc tatatcaacc
     1321 gcatcgtgga cgtggccagc caggtggctc tgtacacctt cggccatcgg gccaacgagt
     1381 gggagaaaac tgatgtggaa ggaaccttat ttgtttatac aaggtctgct tctccaaagc
     1441 atggattcac cattatgaat aggctgagca tggaaaatag gacagaacct attactaaag
     1501 acttggattt ccaactccag gaccctttcc ttctctacag aaatgccaga ttgtccatct
     1561 atggaatttg gttttatgat aaggaagaat gccaaagaat tgcagagctt atgaaaaacc
     1621 taactcagta tgaacagttg aaagcccatc agggaactgg agcaggaatt tcaccagtga
     1681 tcctcaattc aggagagggc aaagaagtag acattttacg aatgctcatc aaggccaaag
     1741 acgaatacac aaagtgtaaa acctgttctg agccaaaaaa gataaccagt tcctctgcca
     1801 tctatgacaa tccaaatctc atcaaaccaa ttccagtgaa acccagtgaa aaccagcaac
     1861 agcgtatacc tcagcccaac cagaccttag accctgaacc ccaacactta tccttgacag
     1921 ctctgtttgg gaagcaggac aaagctacat gtcaggaaac tgtggagcct ccgcagactc
     1981 tccaccagca gcagcagcag cagcagcagc agcagcaaga gaagcttcca attaggcagg
     2041 gggttgtacg ctccctgtcc tatgaggaac ccagaagaca ctcacccccc attgagaagc
     2101 agctctgtcc agccattcag aaactcatgg tcaggagcgc agacctccac ccattgtcag
     2161 agctgcctga aaaccggcct tgtgaaaatg gcagtaccca ttctgcggga gaatttttta
     2221 caggacctgt ccagccaggg tctcctcaca acattggaac ttctcgtggt gtacaaaatg
     2281 cttccagaac tcagaacctg ttcgagaaac ttcagagtac cccaggggca gcaaacaagt
     2341 gtgaccctag tacaccagca cctgccagct cagctgccct gaaccgcagc agagctccca
     2401 cttctgtcac ccctgtggct ccaggaaagg gtctggctca gccaccacag gcctatttca
     2461 atggctccct tccacctcag acagtaggac atcaggctca tggaagagaa cagtccacac
     2521 tcccaagaca aacactcgcc atctctggta gtcagactgg cagctctgga gtgatctccc
     2581 ctcaagagtt actgaagaag cttcagattg tacagcagga gcagcagctg catgcctcta
     2641 accggccagc cttggccgct aagtttcctg tgctcgctca gagctctgga acagggaaac
     2701 ccttggaatc ctggatcaac aagacaccca acacagaaca gcagactcct cttttccagg
     2761 tcatctcacc tcagcgcatc cctgctacag cagctccgtc tctgcttatg tcccccatgg
     2821 tgttcgcaca acccacctcc gtcccgccaa aggaaaggga gagcggcctc ttgcctgtgg
     2881 gaggccagga gccacctgtt gctgccacca gcctcctcct gcccatacag agcccggagc
     2941 cctccgtgat caccagcagc ccactcacca agctccagct ccaggaggca ctgctgtacc
     3001 tcattcagaa tgatgacaac ttcttaaata taatctatga agcctatctc ttcagcatga
     3061 ctcaagcagc catgaaaaag actatgtgag gtaccgcggg cccgggatcc accggatcta
     3121 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     3181 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     3241 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3301 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
     3361 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     3421 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     3481 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     3541 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     3601 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     3661 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     3721 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     3781 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     3841 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3901 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     3961 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     4021 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     4081 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     4141 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4201 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     4261 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     4321 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     4381 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     4441 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     4501 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     4561 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     4621 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     4681 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     4741 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     4801 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     4861 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     4921 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     4981 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     5041 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     5101 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     5161 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     5221 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     5281 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     5341 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     5401 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     5461 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     5521 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     5581 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5641 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5701 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5761 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5821 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5881 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5941 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6001 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6061 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6121 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6181 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6241 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6301 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6361 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6421 tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta
     6481 gttcatagcc catatatgga gttccg
//
