LOCUS       sequence-306036-        6429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent protein fused to mRNA decapping enzyme 1a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Lee JE, Cathey PI, Wu H, Parker R, Voeltz GK
  TITLE     Endoplasmic reticulum contact sites regulate the dynamics of 
            membraneless organelles.
  JOURNAL   Science. 2020 Jan 31;367(6477). pii: 367/6477/eaay7108. doi: 
            10.1126/science.aay7108.
  PUBMED    32001628
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2020-01-31"; volume: "367(6477). pii: 367/6477/eaay7108. 
            doi"; pages: " 10.1126/science.aay7108"
COMMENT     Sequence Label: BFP-Dcp1a
FEATURES             Location/Qualifiers
     source          1..6429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        127..318
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     275..295
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(320..424)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      451..906
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(538..557)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     748..769
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    913..1034
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(924..943)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     978..997
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2946..3644)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        complement(3683..3886)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3712..3732)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3887..4190
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(4365..4953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4445..4464)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5282..5329
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5354..5373
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5561..6355)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5561..6355)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5672..5691)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6282..6301
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(6390..6429,1..318))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(6404..6429,1..110)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
       61 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
      121 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
      181 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
      241 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attccacagc
      301 tggttctttc cgcctcagga ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      361 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      421 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
      481 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      541 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      601 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
      661 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
      721 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
      781 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
      841 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
      901 caatttacgc gttaagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
      961 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     1021 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     1081 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     1141 agttatctag atccggtgga tcccgggccc gcggtacctc ataggttgtg gttgtctttg
     1201 ttcttggtca gcacctgcaa gtagacttca tgaagtgtac tgaggaagct ggaatcattc
     1261 tttattagat gtattaatgt atcctggagc tgagacttgc tgagaataat ggaaggcttt
     1321 ctctgacttt ctggcgttcc aatagttaga ggagaagggg agctggcttt cctctcaagg
     1381 tccgaagatc ttgtaactgt ctgctggaaa acacttgggg ccagcaggac tgaagacact
     1441 gcagtggtcg ttgcagtaac cagtggggcg cctgcaaccg ggatggcact ggataacacc
     1501 ttaggctgca caaatacttc aggatcctgg ttctgctgca tagactgaag gggagcaagc
     1561 accatgttgc tcagggaggc tgaggccgcc actcttgctg ctgctgtctt agagggaggc
     1621 tctatgaagc tctcaggtgt ggctagctga ccagctgccg gagaaaagct ggctaccatt
     1681 gcacctttcc caagtggttg tgtctgtatt tggtcatgct gtggggtcaa cctgagtttc
     1741 tggagaagat caacgcttgg gagggatgtg ccagctgtgt tcgtcacgtt caatggggcc
     1801 ctgaaggggc tctggttggc aatcagactg gcatggctta gctcagggac tggctggttc
     1861 aggagtggag acctctgtct aggcgtggtc ttcactgcct gcatcatggt gctgtttcga
     1921 ggtaagctgg ggggaacctg tgcagtagga gcttcagctg gcagagtggg actgagaaca
     1981 gggctcaacg ggattgtgta ggttggagca tgcttttcat tggactgtgt gatggaggct
     2041 ggagtgatta gcaccggggt ggtgatttca ggctggactg aatggtgggc agcagaaggg
     2101 acacccaggg tttctgattg aggggctcct cctaactgct caaagggaaa tggtaggaat
     2161 gaattgggct ctttctggga ggcatctcct ggcaacctct ccatttcttc tgaatccaga
     2221 cccacaactg ctggttgttc ctttggcaaa gaggttccaa ataactcttc taccgtcaga
     2281 tgcttgtgtc cagatggagc agacttatcc tgtgacccgg agggcatttc ttctagagtc
     2341 tcggtgcttc ccagattgga gagctgagtg cttggctgta acccagggct ggagatattt
     2401 gagtcaccca tctgattcct ctcatactca tccttggctc tgctcagcat ctccaggatg
     2461 tcgatgggcc tgtggtcgct gcagccattg gcctggctgg gactctgttt gtcccgagca
     2521 gcttgctggg atcgccgtgt ctcctcttct accacatcag ccatgagttt tgctatgcgg
     2581 tgacagtcat tcttgtcata aaaccagata ctatatatcg acaagcttgc atttctatac
     2641 agaagaaatg gttcatggag ctgaaattcc aaatctttat tcactggttc aactagattg
     2701 tgcatattta gtcgattcac aatggtaaaa ccatggtaag gggaagctga ccttcgatat
     2761 acgaataagg tcccttctat atcagtcttc tcccactggt tggccttggg gcagaaggtg
     2821 tacagagcga cctggcccgt gaggtctgcg atgctggtga tataggggtc gtgttgcttc
     2881 agggccgcta ggctcatctc ctgcccagct cgactcagcg cctcagctcg agatctgagt
     2941 ccggaattaa gcttgtgccc cagtttgcta gggaggtcgc agtatctggc cactgccacc
     3001 tcgtgctgct cgacgtaggt ctcgttgttg gcctccttga ttctttccag tctgtagtcc
     3061 acatagtaga cgccaggcat cttgaggttc ttagcgggtt tcttggatct atatgtggtc
     3121 ttgatgtttg cgatcagatg gctcccgccc acgagcttca gggccatgtc gtttctgcct
     3181 tccaggccgc cgtcagcggg gtacagcgtc tcggtgaagg cctcccagcc gagtgttttc
     3241 ttctgcatca cagggccgtt ggatgtgaag ttcacccctc tgatcttgac gttgtagatg
     3301 aggcagccgt cctggaggct ggtgtcctgg gtagcggtca gcacgccccc gtcttcgtat
     3361 gtggtgactc tctcccatgt gaagccctca gggaaggact gcttgaagaa gtcggggatg
     3421 ccctgggtgt ggttgatgaa ggtcttgctg ccgtagagga agctagtagc caggatgtcg
     3481 aaggcgaagg ggagagggcc gccctcgacc accttgattc tcatggtctg ggtgccctcg
     3541 tagggcttgc cttcgccctc ggatgtgcac ttgaagtgat ggttgtccac ggtgccctcc
     3601 atgtacagct tcatgtgcat gttctcctta atcagctcgc tcatggtggc gaccgctagc
     3661 ggatctgacg gttcactaaa ccagctctgc ttatatagac ctcccaccgt acacgcctac
     3721 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
     3781 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     3841 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
     3901 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
     3961 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     4021 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     4081 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     4141 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     4201 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta atgcatggcg
     4261 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4321 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4381 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4441 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4501 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4561 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4621 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4681 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4741 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4801 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4861 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4921 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4981 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     5041 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     5101 tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg
     5161 ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc
     5221 caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta
     5281 tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
     5341 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag
     5401 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
     5461 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
     5521 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     5581 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     5641 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     5701 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     5761 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     5821 gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     5881 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     5941 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     6001 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     6061 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     6121 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt
     6181 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     6241 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     6301 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     6361 acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct
     6421 cctcactac
//
