LOCUS       sequence-306039-       14607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes p40S:QfDBD-QF2AD-GFP-NLS synthetic transcription factor, 
            activating DsRed-SKL with promoter 5xQUAS+CP_pcbC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14607)
  AUTHORS   Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
  TITLE     Synthetic control devices for gene regulation in Penicillium 
            chrysogenum.
  JOURNAL   Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 
            10.1186/s12934-019-1253-3.
  PUBMED    31739777
REFERENCE   2  (bases 1 to 14607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2019-11-18"; volume: "18(1):203. doi"; pages: " 
            10.1186/s12934-019-1253-3"
COMMENT     Sequence Label: pLM2_36_p40S_QfDBD_Qf2AD_cp_pcbC_DsRed_5xQUAS
FEATURES             Location/Qualifiers
     source          1..14607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3270..3272,3273..3275,3276..3986)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3270..3272,3273..3275,3276..3986)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3315..3336)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3576..3595)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3923..3944
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3987..4007
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          5179..5294
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             7877..8551
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(8007..8025)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(8057..8077)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8469..8492
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    10385..10409
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      10497..11207
                     /label=oriV
                     /note="incP origin of replication"
     promoter        11228..11332
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11333..12127
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12298..12886
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12787..12806
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             13222..14370
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14516..14540
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
       61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
      121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
      181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
      241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
      301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
      361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
      421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
      481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
      541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
      601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
      661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
      721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
      781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
      841 gaattcaagc ttggaggagt tatagacggt ccggcatagg taaggagaga ttagatattt
      901 cgacttcccg attccttagt ctccttagtc tggaaactga gaaaggcctg aactcgttca
      961 atctattagc tctaatcacg tagtctcaac tagaatcctg aacaggcgcc ggaaccagtc
     1021 caaatgcgtg gagtacagca agtccagtca aatcacgatt agaatggcca tatataagca
     1081 aaaaaccctt ggacaatacg tccgatcgac accaaagcaa tctggtacat cacccttctt
     1141 atatacaggg tagccggaat tgtattgact ctatacgaag ccgcaggttt accagcacag
     1201 aggtgcgata gctacacgaa tgtagagtga aggcattaca accgattgct aagtgtcata
     1261 gaggctaagc ttcacggctc aaggagtctg tggaggatac ctgatataag aattgagagt
     1321 tatactccgg aggttatgtt agtacctcaa aaatgtgaag tacaacgaaa ctcgaacttc
     1381 gaatgacaat agcttggctg gctgttagtc aactgaggta caacgtcatc ttgcaattag
     1441 ttatgttctt tgctagatag tgagctgctg taaacttgta gttcttagaa acatgtctga
     1501 agtctagagt cgagacttgt gattggtgta aattagtggg ggtccggagc ggaataacaa
     1561 tcgaggtcag cctcctgggt ctgtcagtaa gctaaccacg aaaggacaag cgtagacgag
     1621 gtcacggggt actggcctag ccggcttcgg ctagcgccca cgaaaatagg gcaacgtcga
     1681 agcgagagac acacacggat tccacgatcg ccgacgacaa gcactcaact ccacgacaac
     1741 ccacgacaac ccttgccgct gcgttcttgg tgaagggctg caggattaga ggtgagtatt
     1801 ttattttctg gctattgtga tctttttccc ccccggtttc cgctggttct atcttgtcga
     1861 gcgagtcggt gcgccttcga tcggtctgtc aaatttccat ccgtccactc gttcacccgg
     1921 tatcgataca agagggaatg gagcattacg acaggggagc agaggcagaa ctctcgatgt
     1981 tgctactttt tttgcttgga acaacttcct tgaaagattc ttttctgaat gcggctggga
     2041 aactgcgaat tggatatcca gcgaaaggtt accgacagac tcacggccat cgataccact
     2101 cagcgggata gaattgaagg cattcggaca ttagatgatc agcttcgctt gtttcgactc
     2161 gtgctaatca attatccctg gacagtcccc cacatagaca gcaaacaatg ccacccaagc
     2221 gcaaaacgct taacgctgcg gctgaggcta acgctcatgc cgacggacac gccgacggaa
     2281 acgccgacgg acacgtggcc aatacggccg cgtcctcgaa taatgcgagg ttcgctgatc
     2341 tcactaacat cgatactccg ggtctgggac ccacaactac gaccctgctc gtggaaccag
     2401 cacgctcaaa gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca gcccgagaga
     2461 aatgcgacgg aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg tcctgcactt
     2521 atcaggcttc gccgaaaaag aggggagttc aaaccggtta tattcgtacg ctggagctcg
     2581 ccctcgcctg gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat aacctcctcg
     2641 tccgtgacgc cggacaagga tcagctctgc tcgttggtaa agattcgccg gctgccgagc
     2701 gactccatgc ccgttgggct actagccgtg tcaataagag cattacccgc ctcctcaggt
     2761 ttcgtcagtt ggagctccct cctaccgcca cggctacggc ctcgataatg ccgcacgtga
     2821 tggagcagcc tctcagtacc agcattaacc ccgtcaacga ccgcttcaac ggtattccca
     2881 accccactcc gtataactcc gatgcagctc tcgatgctat cactcagacc aacgattatg
     2941 gaagcgtaaa tacacatggt atcctctcta cttacccgcc accggctacg caccttaatg
     3001 aagcttccgt cgctctcgct cccggtggcg cccccccccg accgcctcct ccgtatgttg
     3061 acagcacgac caatcacccg ccgtaccact cgaatctggt tccaatggcg aactttggtt
     3121 actcgaccgt tgattacgat gccatggttg acgatttggc tagcattgaa tacacggacg
     3181 ctgtggatgt cgacccacag tttatgacca atctgggatt cgttcctgga tgtaacttct
     3241 ccgacattaa tacatacgaa cagttcgtta tggtgagcaa gggcgaggag ctgttcaccg
     3301 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     3361 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     3421 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     3481 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     3541 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     3601 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     3661 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     3721 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     3781 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     3841 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     3901 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3961 ctctcggcat ggacgagctg tacaagccaa agaagaagcg gaaggtctaa gcttacttct
     4021 ttatcggttc tctcttacga ctttttgaat ggaacgtttc cttcttctca ggcgggccta
     4081 tctttgggcc gaagctcttt tccttgtact gtaggacctg gttgataatg attcccaaaa
     4141 agacatccag catgtcagtt acttgcattc gtcagtctat acaaaagcaa tggtttagag
     4201 aaattttgaa ctttatacat ggttttattt gttgcttcac ggccgtacct tctggaaatc
     4261 cacggtagga gtgtcaattt gcgtttttga taatccttcc aaggttcttc tcgaagtagt
     4321 tgttctataa ttgcttcaca gctaccatgg aacatcccaa gcaccgctac tagaattcga
     4381 gctcggagtg gatcccccgg gctgcaggaa ttcgagctct gtacagtgac cggtgactct
     4441 ttctggcatg cggagagacg gacggacgca gagagaaggg ctgagtaata agcgccactg
     4501 cgccagacag ctctggcggc tctgaggtgc agtggatgat tattaatccg ggaccggccg
     4561 cccctccgcc ccgaagtgga aaggctggtg tgcccctcgt tgaccaagaa tctattgcat
     4621 catcggagaa tatggagctt catcgaatca ccggcagtaa gcgaaggaga atgtgaagcc
     4681 aggggtgtat agccgtcggc gaaatagcat gccattaacc taggtacaga agtccaattg
     4741 cttccgatct ggtaaaagat tcacgagata gtaccttctc cgaagtaggt agagcgagta
     4801 cccggcgcgt aagctcccta attggcccat ccggcatctg tagggcgtcc aaatatcgtg
     4861 cctctcctgc tttgcccggt gtatgaaacc ggaaaggccg ctcaggagct ggccagcggc
     4921 gcagaccggg aacacaagct ggcagtcgac ccatccggtg ctctgcactc gacctgctga
     4981 ggtccctcag tccctggtag gcagctttgc cccgtctgtc cgcccggtgt gtcggcgggg
     5041 ttgacaaggt cgttgcgtca gtccaacatt tgttgccata ttttcctgct ctccccacca
     5101 gctgctcttt tcttttctct ttcttttccc atcttcagta tattcatctt cccatccaag
     5161 aacctttatt tcccctaagt aagtactttg ctacatccat actccatcct tcccatccct
     5221 tattcctttg aacctttcag ttcgagcttt cccacttcat cgcagcttga ctaacagcta
     5281 ccccgcttga gcagacatca ccatgcctca atcctgggaa gaactggccg ctgataagcg
     5341 cgcccgcctc gcaaaaacca tccctgatga atggaaagtc cagacgctgc ctgcggatga
     5401 cagcgttatt gatttcccaa agaaatcggg gatcctttca gaggccgaac tgaagatcac
     5461 agaggcctcc gctgcagatc ttgtgtccaa gctggcggcc ggagagttga cctcggtgga
     5521 agttacgcta gcattctgta aacgggcagc aatcgcccag cagttagtag ggtcccctct
     5581 acctctcagg gagatgtaac aacgccacct tatgggacta tcaagctgac gctggcttct
     5641 gtgcagacaa actgcgccca cgagttcttc cctgacgccg ctctcgcgca ggcaagggaa
     5701 ctcgatgaat actacgcaaa gcacaagaca cccgttggtc cactccatgg cctccccatc
     5761 tctctcaaag accagcttcg agtcaaggta caccgttgcc cctaagtcgt tagatgtccc
     5821 tttttgtcag ctaacatatg ccaccagggc tacgaaacat caatgggcta catctcatgg
     5881 ctaaacaagt acgacgaagg ggactcggtt ctgacaacca tgctccgcaa agccggtgcc
     5941 gttttctacg tcaagacctc tgtcccgcag accctgatgg tctgcgagac agtcaacaac
     6001 atcatcgggc gcaccgtcaa cccacgcaac aagaactggt cgtgcggcgg cagttctggt
     6061 ggtgagggtg cgatcgttgg gattcgtggt ggcgtcatcg gtgtaggaac ggatatcggt
     6121 ggctcgattc gagtgccggc cgcgttcaac ttcctgtacg gtctaaggcc gagtcatggg
     6181 cggctgccgt atgcaaagat ggcgaacagc atggagggtc aggagacggt gcacagcgtt
     6241 gtcgggccga ttacgcactc tgttgagggt gagtccttcg cctcttcctt cttttcctgc
     6301 tctataccag gcctccactg tcctcctttc ttgcttttta tactatatac gagacgggca
     6361 gtcactgatg aagtaatgtt agacctccgc ctcttcacca aatccgtcct cggtcaggag
     6421 ccatggaaat acgactccaa ggtcatcccc atgccctggc gccagtccga gtcggacatt
     6481 attgcctcca agatcaagaa cggcgggctc aatatcggct actacaactt cgacggcaat
     6541 gtccttccac accctcctat cctgcgcggc gtggaaacca ccgtcgccgc actcgccaaa
     6601 gccggtcaca ccgtgacccc gtggacgcca tacaagcacg atttcggcca cgatctcatc
     6661 tcccatatct acgcggctga cggcagcgcc gacgtaatgc gcgatatcag tgcatccggc
     6721 gagccggcga ttccaaatat caaagaccta ctgaacccga acatcaaagc tgttaacatg
     6781 aacgagctct gggacacgca tctccagaag tggaattacc agatggagta ccttgagaaa
     6841 tggcgggagg ctgaagaaaa ggccgggaag gaactggacg ccatcatcgc gccgattacg
     6901 cctaccgctg cggtacggca tgaccagttc cggtactatg ggtatgcctc tgtgatcaac
     6961 ctgctggatt tcacgagcgt ggttgttccg gttacctttg cggataagaa catcgataag
     7021 aagaatgaga gtttcaaggc ggttagtgag cttgatgccc tcgtgcagga agagtatgat
     7081 ccggaggcgt accatggggc accggttgca gtgcaggtta tcggacggag actcagtgaa
     7141 gagaggacgt tggcgattgc agaggaagtg gggaagttgc tgggaaatgt ggtgactcca
     7201 taggcttcta ataagtgtca gatagcaatt tgcacaagaa atcaatacca gcaactgtaa
     7261 ataagcgctg aagtgaccat gccatgctac gaaagagcag aaaaaaacct gccgtagaac
     7321 cgaagagata tgacacgctt ccatctctca aaggaagaat cccttcaggg ttgcgtttcc
     7381 agtctagaca cgtataacgg cacaagtgtc tctcaccaaa tgggttatat ctcaaatgtg
     7441 atctaaggat ggaaagccca gaatattggc tgggttgatg gctgcttcga gtgcagtctc
     7501 atgctgccac aggtgactct ggatggcccc ataccactca acccatggta cgagcggtac
     7561 gctttacgaa ttcccatggg gaggggtaat cgattatcct cggataaaca cttatcccag
     7621 ggtaatcact tatccctgga taaacaatta tcctcgggta atcgcttatc cccatcgtat
     7681 aatgtctcca ggttgtctca gcataaacac cccgcccccg ctcaggcaca caggaagaga
     7741 gctcaggtcg tttccattgc gtccatactc ttcactcatt gtcatctgca ggagaacttc
     7801 ccctgtccct ttgccaagcc ctctcttcgt cgttgtccac gccttcaagt tttcaccatt
     7861 atttttctag acaccaatgg cctcctccga ggacgtcatc aaggagttca tgcgcttcaa
     7921 ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     7981 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
     8041 cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt acgtgaagca
     8101 ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca agtgggagcg
     8161 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     8221 cggctccttc atctacaagg tgaagttcat cggcgtgaac ttcccctccg acggccccgt
     8281 aatgcagaag aaaactatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg
     8341 cgtgctgaag ggcgagatcc acaaggccct gaagctgaag gacggcggcc actacctggt
     8401 ggagttcaag tccatctaca tggccaagaa gcccgtgcag ctgcccggct actactacgt
     8461 ggactccaag ctggacatca cctcccacaa cgaggactac accatcgtgg agcagtacga
     8521 gcgcgccgag ggccgccacc acctgttcct gactcacggt atggacgaat tgtacaagtc
     8581 gaagctgtaa gcttgtgctt ctaaggtatg agtcgcaaaa ttgtttttta tttttggtct
     8641 tgagtctaat atgctcgcag ctcttgcgtt gtatatggtc gttgtcgcgt attttctgtt
     8701 gtattaaaag atcaaacgag atcaagggat ggctcgcggg ctgtctctcg cactaggagg
     8761 aagaatgcct gaaaaaggaa ctttgatttt agctgtggaa tagagatggc ttgtttgagg
     8821 acgcttgtcg cttggcgcag ggacttgaat ggcagcttgt ggaaaccgaa ggcgagaaaa
     8881 gtcgacggat actgtacgtg gttctattgc cagtgcggtg gaagcttcct tctcatatag
     8941 ttcaatcctt ctttgaatct gtttgtttca tatttggact gtttcattct ctgcttgcgc
     9001 attctcatct tcgagaacga ctgcagggat tgttggttct gtggagctga tgagcgcgcc
     9061 ttgaccaccc ttgttcttgt tttgctcttt tgttctcatt taacccgttt ctcccttcca
     9121 accctttgac cttgcaacat tgtctcccag cgcgttgcca aagcgaactt gatatcagta
     9181 tagtatgacc aagtagtcta ccaaaataaa ttttagtaca gtattgctag ttatacaaga
     9241 taaattttgt acaacaatga ttatgcagag ctggtattct gtaaggagta aattcatgca
     9301 ggtacagagc gtccacgtca ggcacacgcg ctttagtgct gggtacacat tatgctacct
     9361 ttggggcaag gtgctattct aggtagggta tgcctagcaa tgccatgatc ttatgacctc
     9421 agtaatctga ccttggattt aagatcggag ttctcaaacc ctgcgcgctc agagaattcg
     9481 catgcggaga ctttagacat acctctcgtt gccgacaaat cggcattccg tttcggtcac
     9541 ggacgtaaga gttcaacaaa gccgctcacc tgtcccgttc ggcgcttgag tttccacttc
     9601 aaaagcacaa ccgctccgat atttccattg ccaccgacca acaaaaggtt catatcgcgc
     9661 aatagtcgtc caactctcac tccgcccgca ctcgatggcg aaggatggaa gaatatccga
     9721 gcggcatcgg ataaagagat ctggttcttt tcgtgaagta acgtagggaa ccgacggaaa
     9781 ctgtagagct ataggaattt agtatatcag aagtcaattg agagaagtgt atagttagta
     9841 actcacttgt gccaacaggt atttccaacc gttcttgttc aaatttcgtc aagtcaccat
     9901 ctctatgtgc aaaactggat catacaacag ctcttattgt agctgcatct cgctctcaga
     9961 gatcatccta tcttcgtcgt catccaccaa cacaggtggg agtgctagtt gatccatgct
    10021 gaggtcaagg gacacagacg gaatgggtgt agaacgcctc aaaacaggta aggtatcagg
    10081 atcggttgtc cagggggaat ccaaaccagg ttccaccttg attcagagaa taagtatgac
    10141 gtattcttgg caatgtttaa gcttgagatg agccgatgtc aaactgggag acatgatgaa
    10201 ggaggaaatg ttgctttggt gggggagaca ctataaatac caaagagtga atggttgata
    10261 ccatttgggg aaggagtgtc tatcgcagtc tctgcagacg gcatatcgga tagcctgcag
    10321 gcgcttgtgg aggatgcaca tgtgaccgag ggacacgaag tgatccgttt aaactatcag
    10381 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
    10441 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gccagccgcc
    10501 tttgcgacgc tcaccgggct ggttgccctc gccgctgggc tggcggccgt ctatggccct
    10561 gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac accgcggccg ccggcgttgt
    10621 ggatacctcg cggaaaactt ggccctcact gacagatgag gggcggacgt tgacacttga
    10681 ggggccgact cacccggcgc ggcgttgaca gatgaggggc aggctcgatt tcggccggcg
    10741 acgtggagct ggccagcctc gcaaatcggc gaaaacgcct gattttacgc gagtttccca
    10801 cagatgatgt ggacaagcct ggggataagt gccctgcggt attgacactt gaggggcgcg
    10861 actactgaca gatgaggggc gcgatccttg acacttgagg ggcagagtgc tgacagatga
    10921 ggggcgcacc tattgacatt tgaggggctg tccacaggca gaaaatccag catttgcaag
    10981 ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt aacctgcttt taaaccaata
    11041 tttataaacc ttgtttttaa ccagggctgc gccctgtgcg cgtgaccgcg cacgccgaag
    11101 gggggtgccc ccccttctcg aaccctcccg gcccgctaac gcgggcctcc catcccccca
    11161 ggggctgcgc ccctcggccg cgaacggcct caccccaaaa atggcagcgc tggccaattc
    11221 ccgagtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    11281 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatggctaa
    11341 aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac
    11401 ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt
    11461 aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat
    11521 gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga
    11581 tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga
    11641 agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca
    11701 ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt
    11761 ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg aagaggacac
    11821 tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga
    11881 acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt
    11941 aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt
    12001 ctgcgtccgg tcgctcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga
    12061 cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt
    12121 gttttagctg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
    12181 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    12241 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    12301 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    12361 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    12421 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    12481 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    12541 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    12601 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    12661 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    12721 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    12781 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    12841 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    12901 ggccttttta cggttcctgg cagatcctag atgtggcgca acgatgccgg cgacaagcag
    12961 gagcgcaccg acttcttccg catcaagtgt tttggctctc aggccgaggc ccacggcaag
    13021 tatttgggca aggggtcgct ggtattcgtg cagggcaaga ttcggaatac caagtacgag
    13081 aaggacggcc agacggtcta cgggaccgac ttcattgccg ataaggtgga ttatctggac
    13141 accaaggcac caggcgggtc aaatcaggaa taagggcaca ttgccccggc gtgagtcggg
    13201 gcaatcccgc aaggagggtg aatgaatcgg acgtttgacc ggaaggcata caggcaagaa
    13261 ctgatcgacg cggggttttc cgccgaggat gccgaaacca tcgcaagccg caccgtcatg
    13321 cgtgcgcccc gcgaaacctt ccagtccgtc ggctcgatgg tccagcaagc tacggccaag
    13381 atcgagcgcg acagcgtgca actggctccc cctgccctgc ccgcgccatc ggccgccgtg
    13441 gagcgttcgc gtcgtcttga acaggaggcg gcaggtttgg cgaagtcgat gaccatcgac
    13501 acgcgaggaa ctatgacgac caagaagcga aaaaccgccg gcgaggacct ggcaaaacag
    13561 gtcagcgagg ccaagcaggc cgcgttgctg aaacacacga agcagcagat caaggaaatg
    13621 cagctttcct tgttcgatat tgcgccgtgg ccggacacga tgcgagcgat gccaaacgac
    13681 acggcccgct ctgccctgtt caccacgcgc aacaagaaaa tcccgcgcga ggcgctgcaa
    13741 aacaaggtca ttttccacgt caacaaggac gtgaagatca cctacaccgg cgtcgagctg
    13801 cgggccgacg atgacgaact ggtgtggcag caggtgttgg agtacgcgaa gcgcacccct
    13861 atcggcgagc cgatcacctt cacgttctac gagctttgcc aggacctggg ctggtcgatc
    13921 aatggccggt attacacgaa ggccgaggaa tgcctgtcgc gcctacaggc gacggcgatg
    13981 ggcttcacgt ccgaccgcgt tgggcacctg gaatcggtgt cgctgctgca ccgcttccgc
    14041 gtcctggacc gtggcaagaa aacgtcccgt tgccaggtcc tgatcgacga ggaaatcgtc
    14101 gtgctgtttg ctggcgacca ctacacgaaa ttcatatggg agaagtaccg caagctgtcg
    14161 ccgacggccc gacggatgtt cgactatttc agctcgcacc gggagccgta cccgctcaag
    14221 ctggaaacct tccgcctcat gtgcggatcg gattccaccc gcgtgaagaa gtggcgcgag
    14281 caggtcggcg aagcctgcga agagttgcga ggcagcggcc tggtggaaca cgcctgggtc
    14341 aatgatgacc tggtgcattg caaacgctag ggccttgtgg ggtcagttcc ggctgggggt
    14401 tcagcagccc ctgctcggat ctgttggacc ggacagtagt catggttgat gggctgcctg
    14461 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
    14521 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
    14581 ttttaatgta ctggggttga acactct
//
