LOCUS       sequence-306041-       14041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, 
            activating DsRed-SKL with promoter CP_nirA (no QUAS control).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14041)
  AUTHORS   Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
  TITLE     Synthetic control devices for gene regulation in Penicillium 
            chrysogenum.
  JOURNAL   Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 
            10.1186/s12934-019-1253-3.
  PUBMED    31739777
REFERENCE   2  (bases 1 to 14041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2019-11-18"; volume: "18(1):203. doi"; pages: " 
            10.1186/s12934-019-1253-3"
COMMENT     Sequence Label: pLM2_32_p40S_QfDBD_VP16AD_cp_nirA_DsRed_0xQUAS
FEATURES             Location/Qualifiers
     source          1..14041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2781..2891
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2949..2970)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3210..3229)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3557..3578
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3621..3641
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4813..4928
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             7311..7985
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(7441..7459)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7491..7511)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     7903..7926
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    9819..9843
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      9931..10641
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10662..10766
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10767..11561
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11732..12320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12221..12240
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12656..13804
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    13950..13974
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
       61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
      121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
      181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
      241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
      301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
      361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
      421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
      481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
      541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
      601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
      661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
      721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
      781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
      841 gaattcaagc ttggaggagt tatagacggt ccggcatagg taaggagaga ttagatattt
      901 cgacttcccg attccttagt ctccttagtc tggaaactga gaaaggcctg aactcgttca
      961 atctattagc tctaatcacg tagtctcaac tagaatcctg aacaggcgcc ggaaccagtc
     1021 caaatgcgtg gagtacagca agtccagtca aatcacgatt agaatggcca tatataagca
     1081 aaaaaccctt ggacaatacg tccgatcgac accaaagcaa tctggtacat cacccttctt
     1141 atatacaggg tagccggaat tgtattgact ctatacgaag ccgcaggttt accagcacag
     1201 aggtgcgata gctacacgaa tgtagagtga aggcattaca accgattgct aagtgtcata
     1261 gaggctaagc ttcacggctc aaggagtctg tggaggatac ctgatataag aattgagagt
     1321 tatactccgg aggttatgtt agtacctcaa aaatgtgaag tacaacgaaa ctcgaacttc
     1381 gaatgacaat agcttggctg gctgttagtc aactgaggta caacgtcatc ttgcaattag
     1441 ttatgttctt tgctagatag tgagctgctg taaacttgta gttcttagaa acatgtctga
     1501 agtctagagt cgagacttgt gattggtgta aattagtggg ggtccggagc ggaataacaa
     1561 tcgaggtcag cctcctgggt ctgtcagtaa gctaaccacg aaaggacaag cgtagacgag
     1621 gtcacggggt actggcctag ccggcttcgg ctagcgccca cgaaaatagg gcaacgtcga
     1681 agcgagagac acacacggat tccacgatcg ccgacgacaa gcactcaact ccacgacaac
     1741 ccacgacaac ccttgccgct gcgttcttgg tgaagggctg caggattaga ggtgagtatt
     1801 ttattttctg gctattgtga tctttttccc ccccggtttc cgctggttct atcttgtcga
     1861 gcgagtcggt gcgccttcga tcggtctgtc aaatttccat ccgtccactc gttcacccgg
     1921 tatcgataca agagggaatg gagcattacg acaggggagc agaggcagaa ctctcgatgt
     1981 tgctactttt tttgcttgga acaacttcct tgaaagattc ttttctgaat gcggctggga
     2041 aactgcgaat tggatatcca gcgaaaggtt accgacagac tcacggccat cgataccact
     2101 cagcgggata gaattgaagg cattcggaca ttagatgatc agcttcgctt gtttcgactc
     2161 gtgctaatca attatccctg gacagtcccc cacatagaca gcaaacaatg ccacccaagc
     2221 gcaaaacgct taacgctgcg gctgaggcta acgctcatgc cgacggacac gccgacggaa
     2281 acgccgacgg acacgtggcc aatacggccg cgtcctcgaa taatgcgagg ttcgctgatc
     2341 tcactaacat cgatactccg ggtctgggac ccacaactac gaccctgctc gtggaaccag
     2401 cacgctcaaa gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca gcccgagaga
     2461 aatgcgacgg aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg tcctgcactt
     2521 atcaggcttc gccgaaaaag aggggagttc aaaccggtta tattcgtacg ctggagctcg
     2581 ccctcgcctg gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat aacctcctcg
     2641 tccgtgacgc cggacaagga tcagctctgc tcgttggtaa agattcgccg gctgccgagc
     2701 gactccatgc ccgttgggct actagccgtg tcaataagag cattacccgc ctcctcaggt
     2761 ttgaaagcgg cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg
     2821 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     2881 ttgacatgct ccccgggttc gttatggtga gcaagggcga ggagctgttc accggggtgg
     2941 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     3001 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     3061 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     3121 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3181 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3241 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3301 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3361 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3421 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3481 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3541 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3601 gcatggacga gctgtacaag ccaaagaaga agcggaaggt ctaagcttac ttctttatcg
     3661 gttctctctt acgacttttt gaatggaacg tttccttctt ctcaggcggg cctatctttg
     3721 ggccgaagct cttttccttg tactgtagga cctggttgat aatgattccc aaaaagacat
     3781 ccagcatgtc agttacttgc attcgtcagt ctatacaaaa gcaatggttt agagaaattt
     3841 tgaactttat acatggtttt atttgttgct tcacggccgt accttctgga aatccacggt
     3901 aggagtgtca atttgcgttt ttgataatcc ttccaaggtt cttctcgaag tagttgttct
     3961 ataattgctt cacagctacc atggaacatc ccaagcaccg ctactagaat tcgagctcgg
     4021 agtggatccc ccgggctgca ggaattcgag ctctgtacag tgaccggtga ctctttctgg
     4081 catgcggaga gacggacgga cgcagagaga agggctgagt aataagcgcc actgcgccag
     4141 acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc
     4201 cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg
     4261 agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt
     4321 gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg
     4381 atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc
     4441 gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc
     4501 ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac
     4561 cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc
     4621 tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca
     4681 aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct
     4741 cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt
     4801 tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc
     4861 tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc
     4921 ttgagcagac atcaccatgc ctcaatcctg ggaagaactg gccgctgata agcgcgcccg
     4981 cctcgcaaaa accatccctg atgaatggaa agtccagacg ctgcctgcgg atgacagcgt
     5041 tattgatttc ccaaagaaat cggggatcct ttcagaggcc gaactgaaga tcacagaggc
     5101 ctccgctgca gatcttgtgt ccaagctggc ggccggagag ttgacctcgg tggaagttac
     5161 gctagcattc tgtaaacggg cagcaatcgc ccagcagtta gtagggtccc ctctacctct
     5221 cagggagatg taacaacgcc accttatggg actatcaagc tgacgctggc ttctgtgcag
     5281 acaaactgcg cccacgagtt cttccctgac gccgctctcg cgcaggcaag ggaactcgat
     5341 gaatactacg caaagcacaa gacacccgtt ggtccactcc atggcctccc catctctctc
     5401 aaagaccagc ttcgagtcaa ggtacaccgt tgcccctaag tcgttagatg tccctttttg
     5461 tcagctaaca tatgccacca gggctacgaa acatcaatgg gctacatctc atggctaaac
     5521 aagtacgacg aaggggactc ggttctgaca accatgctcc gcaaagccgg tgccgttttc
     5581 tacgtcaaga cctctgtccc gcagaccctg atggtctgcg agacagtcaa caacatcatc
     5641 gggcgcaccg tcaacccacg caacaagaac tggtcgtgcg gcggcagttc tggtggtgag
     5701 ggtgcgatcg ttgggattcg tggtggcgtc atcggtgtag gaacggatat cggtggctcg
     5761 attcgagtgc cggccgcgtt caacttcctg tacggtctaa ggccgagtca tgggcggctg
     5821 ccgtatgcaa agatggcgaa cagcatggag ggtcaggaga cggtgcacag cgttgtcggg
     5881 ccgattacgc actctgttga gggtgagtcc ttcgcctctt ccttcttttc ctgctctata
     5941 ccaggcctcc actgtcctcc tttcttgctt tttatactat atacgagacg ggcagtcact
     6001 gatgaagtaa tgttagacct ccgcctcttc accaaatccg tcctcggtca ggagccatgg
     6061 aaatacgact ccaaggtcat ccccatgccc tggcgccagt ccgagtcgga cattattgcc
     6121 tccaagatca agaacggcgg gctcaatatc ggctactaca acttcgacgg caatgtcctt
     6181 ccacaccctc ctatcctgcg cggcgtggaa accaccgtcg ccgcactcgc caaagccggt
     6241 cacaccgtga ccccgtggac gccatacaag cacgatttcg gccacgatct catctcccat
     6301 atctacgcgg ctgacggcag cgccgacgta atgcgcgata tcagtgcatc cggcgagccg
     6361 gcgattccaa atatcaaaga cctactgaac ccgaacatca aagctgttaa catgaacgag
     6421 ctctgggaca cgcatctcca gaagtggaat taccagatgg agtaccttga gaaatggcgg
     6481 gaggctgaag aaaaggccgg gaaggaactg gacgccatca tcgcgccgat tacgcctacc
     6541 gctgcggtac ggcatgacca gttccggtac tatgggtatg cctctgtgat caacctgctg
     6601 gatttcacga gcgtggttgt tccggttacc tttgcggata agaacatcga taagaagaat
     6661 gagagtttca aggcggttag tgagcttgat gccctcgtgc aggaagagta tgatccggag
     6721 gcgtaccatg gggcaccggt tgcagtgcag gttatcggac ggagactcag tgaagagagg
     6781 acgttggcga ttgcagagga agtggggaag ttgctgggaa atgtggtgac tccataggct
     6841 tctaataagt gtcagatagc aatttgcaca agaaatcaat accagcaact gtaaataagc
     6901 gctgaagtga ccatgccatg ctacgaaaga gcagaaaaaa acctgccgta gaaccgaaga
     6961 gatatgacac gcttccatct ctcaaaggaa gaatcccttc agggttgcgt ttccagtcta
     7021 gacacgtata acggcacaag tgtctctcac caaatgggtt atatctcaaa tgtgatctaa
     7081 ggatggaaag cccagaatat tggctgggtt gatggctgct tcgagtgcag tctcatgctg
     7141 ccacaggtga ctctggatgg ccccatacca ctcaacccat ggtacgagcg gtacgcttta
     7201 cgaattccca tggggagcag ggaaacacgc cgagcgtcct cctccgataa gcatgcgctg
     7261 tcttggtctg tcgctgtcac cgaacatatc tgtctgggct tgatttacca atggcctcct
     7321 ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg
     7381 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     7441 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc
     7501 agttccagta cggctccaag gtgtacgtga agcaccccgc cgacatcccc gactacaaga
     7561 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     7621 tggtgaccgt gacccaggac tcctccctgc aggacggctc cttcatctac aaggtgaagt
     7681 tcatcggcgt gaacttcccc tccgacggcc ccgtaatgca gaagaaaact atgggctggg
     7741 aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccacaagg
     7801 ccctgaagct gaaggacggc ggccactacc tggtggagtt caagtccatc tacatggcca
     7861 agaagcccgt gcagctgccc ggctactact acgtggactc caagctggac atcacctccc
     7921 acaacgagga ctacaccatc gtggagcagt acgagcgcgc cgagggccgc caccacctgt
     7981 tcctgactca cggtatggac gaattgtaca agtcgaagct gtaagcttgt gcttctaagg
     8041 tatgagtcgc aaaattgttt tttatttttg gtcttgagtc taatatgctc gcagctcttg
     8101 cgttgtatat ggtcgttgtc gcgtattttc tgttgtatta aaagatcaaa cgagatcaag
     8161 ggatggctcg cgggctgtct ctcgcactag gaggaagaat gcctgaaaaa ggaactttga
     8221 ttttagctgt ggaatagaga tggcttgttt gaggacgctt gtcgcttggc gcagggactt
     8281 gaatggcagc ttgtggaaac cgaaggcgag aaaagtcgac ggatactgta cgtggttcta
     8341 ttgccagtgc ggtggaagct tccttctcat atagttcaat ccttctttga atctgtttgt
     8401 ttcatatttg gactgtttca ttctctgctt gcgcattctc atcttcgaga acgactgcag
     8461 ggattgttgg ttctgtggag ctgatgagcg cgccttgacc acccttgttc ttgttttgct
     8521 cttttgttct catttaaccc gtttctccct tccaaccctt tgaccttgca acattgtctc
     8581 ccagcgcgtt gccaaagcga acttgatatc agtatagtat gaccaagtag tctaccaaaa
     8641 taaattttag tacagtattg ctagttatac aagataaatt ttgtacaaca atgattatgc
     8701 agagctggta ttctgtaagg agtaaattca tgcaggtaca gagcgtccac gtcaggcaca
     8761 cgcgctttag tgctgggtac acattatgct acctttgggg caaggtgcta ttctaggtag
     8821 ggtatgccta gcaatgccat gatcttatga cctcagtaat ctgaccttgg atttaagatc
     8881 ggagttctca aaccctgcgc gctcagagaa ttcgcatgcg gagactttag acatacctct
     8941 cgttgccgac aaatcggcat tccgtttcgg tcacggacgt aagagttcaa caaagccgct
     9001 cacctgtccc gttcggcgct tgagtttcca cttcaaaagc acaaccgctc cgatatttcc
     9061 attgccaccg accaacaaaa ggttcatatc gcgcaatagt cgtccaactc tcactccgcc
     9121 cgcactcgat ggcgaaggat ggaagaatat ccgagcggca tcggataaag agatctggtt
     9181 cttttcgtga agtaacgtag ggaaccgacg gaaactgtag agctatagga atttagtata
     9241 tcagaagtca attgagagaa gtgtatagtt agtaactcac ttgtgccaac aggtatttcc
     9301 aaccgttctt gttcaaattt cgtcaagtca ccatctctat gtgcaaaact ggatcataca
     9361 acagctctta ttgtagctgc atctcgctct cagagatcat cctatcttcg tcgtcatcca
     9421 ccaacacagg tgggagtgct agttgatcca tgctgaggtc aagggacaca gacggaatgg
     9481 gtgtagaacg cctcaaaaca ggtaaggtat caggatcggt tgtccagggg gaatccaaac
     9541 caggttccac cttgattcag agaataagta tgacgtattc ttggcaatgt ttaagcttga
     9601 gatgagccga tgtcaaactg ggagacatga tgaaggagga aatgttgctt tggtggggga
     9661 gacactataa ataccaaaga gtgaatggtt gataccattt ggggaaggag tgtctatcgc
     9721 agtctctgca gacggcatat cggatagcct gcaggcgctt gtggaggatg cacatgtgac
     9781 cgagggacac gaagtgatcc gtttaaacta tcagtgtttg acaggatata ttggcgggta
     9841 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     9901 ttatccgttc gtccatttgt atgtgccagc cgcctttgcg acgctcaccg ggctggttgc
     9961 cctcgccgct gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa
    10021 gccgtgtgcg agacaccgcg gccgccggcg ttgtggatac ctcgcggaaa acttggccct
    10081 cactgacaga tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt
    10141 gacagatgag gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat
    10201 cggcgaaaac gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat
    10261 aagtgccctg cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc
    10321 cttgacactt gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg
    10381 gctgtccaca ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg
    10441 ctaacctgtc ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg
    10501 ctgcgccctg tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct
    10561 cccggcccgc taacgcgggc ctcccatccc cccaggggct gcgcccctcg gccgcgaacg
    10621 gcctcacccc aaaaatggca gcgctggcca attcccgagt gcgcggaacc cctatttgtt
    10681 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    10741 ttcaataata ttgaaaaagg aagagtatgg ctaaaatgag aatatcaccg gaattgaaaa
    10801 aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat
    10861 ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag
    10921 ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc
    10981 ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg
    11041 aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta
    11101 tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct
    11161 atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg
    11221 ccgatgtgga ttgcgaaaac tgggaagagg acactccatt taaagatccg cgcgagctgt
    11281 atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg
    11341 gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa
    11401 gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgctc agggaggata
    11461 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
    11521 agaaaataaa atattatatt ttactggatg aattgtttta gctgtcagac caagtttact
    11581 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    11641 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    11701 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    11761 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    11821 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    11881 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    11941 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    12001 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    12061 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    12121 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    12181 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    12241 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    12301 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcagatc
    12361 ctagatgtgg cgcaacgatg ccggcgacaa gcaggagcgc accgacttct tccgcatcaa
    12421 gtgttttggc tctcaggccg aggcccacgg caagtatttg ggcaaggggt cgctggtatt
    12481 cgtgcagggc aagattcgga ataccaagta cgagaaggac ggccagacgg tctacgggac
    12541 cgacttcatt gccgataagg tggattatct ggacaccaag gcaccaggcg ggtcaaatca
    12601 ggaataaggg cacattgccc cggcgtgagt cggggcaatc ccgcaaggag ggtgaatgaa
    12661 tcggacgttt gaccggaagg catacaggca agaactgatc gacgcggggt tttccgccga
    12721 ggatgccgaa accatcgcaa gccgcaccgt catgcgtgcg ccccgcgaaa ccttccagtc
    12781 cgtcggctcg atggtccagc aagctacggc caagatcgag cgcgacagcg tgcaactggc
    12841 tccccctgcc ctgcccgcgc catcggccgc cgtggagcgt tcgcgtcgtc ttgaacagga
    12901 ggcggcaggt ttggcgaagt cgatgaccat cgacacgcga ggaactatga cgaccaagaa
    12961 gcgaaaaacc gccggcgagg acctggcaaa acaggtcagc gaggccaagc aggccgcgtt
    13021 gctgaaacac acgaagcagc agatcaagga aatgcagctt tccttgttcg atattgcgcc
    13081 gtggccggac acgatgcgag cgatgccaaa cgacacggcc cgctctgccc tgttcaccac
    13141 gcgcaacaag aaaatcccgc gcgaggcgct gcaaaacaag gtcattttcc acgtcaacaa
    13201 ggacgtgaag atcacctaca ccggcgtcga gctgcgggcc gacgatgacg aactggtgtg
    13261 gcagcaggtg ttggagtacg cgaagcgcac ccctatcggc gagccgatca ccttcacgtt
    13321 ctacgagctt tgccaggacc tgggctggtc gatcaatggc cggtattaca cgaaggccga
    13381 ggaatgcctg tcgcgcctac aggcgacggc gatgggcttc acgtccgacc gcgttgggca
    13441 cctggaatcg gtgtcgctgc tgcaccgctt ccgcgtcctg gaccgtggca agaaaacgtc
    13501 ccgttgccag gtcctgatcg acgaggaaat cgtcgtgctg tttgctggcg accactacac
    13561 gaaattcata tgggagaagt accgcaagct gtcgccgacg gcccgacgga tgttcgacta
    13621 tttcagctcg caccgggagc cgtacccgct caagctggaa accttccgcc tcatgtgcgg
    13681 atcggattcc acccgcgtga agaagtggcg cgagcaggtc ggcgaagcct gcgaagagtt
    13741 gcgaggcagc ggcctggtgg aacacgcctg ggtcaatgat gacctggtgc attgcaaacg
    13801 ctagggcctt gtggggtcag ttccggctgg gggttcagca gcccctgctc ggatctgttg
    13861 gaccggacag tagtcatggt tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
    13921 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
    13981 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg ttgaacactc
    14041 t
//
