LOCUS       sequence-306043-       14857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, 
            activating DsRed-SKL with full lenght promoter pcbC .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14857)
  AUTHORS   Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
  TITLE     Synthetic control devices for gene regulation in Penicillium 
            chrysogenum.
  JOURNAL   Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 
            10.1186/s12934-019-1253-3.
  PUBMED    31739777
REFERENCE   2  (bases 1 to 14857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2019-11-18"; volume: "18(1):203. doi"; pages: " 
            10.1186/s12934-019-1253-3"
COMMENT     Sequence Label: pVE2_19_p40S_QfDBD_VP16AD_pFullLength_pcbC_DsRed
FEATURES             Location/Qualifiers
     source          1..14857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2781..2891
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2949..2970)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3210..3229)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3557..3578
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3621..3641
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4813..4928
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             8127..8801
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(8257..8275)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(8307..8327)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8719..8742
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    10635..10659
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      10747..11457
                     /label=oriV
                     /note="incP origin of replication"
     promoter        11478..11582
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11583..12377
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12548..13136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13037..13056
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             13472..14620
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14766..14790
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
       61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
      121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
      181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
      241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
      301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
      361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
      421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
      481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
      541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
      601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
      661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
      721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
      781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
      841 gaattcaagc ttggaggagt tatagacggt ccggcatagg taaggagaga ttagatattt
      901 cgacttcccg attccttagt ctccttagtc tggaaactga gaaaggcctg aactcgttca
      961 atctattagc tctaatcacg tagtctcaac tagaatcctg aacaggcgcc ggaaccagtc
     1021 caaatgcgtg gagtacagca agtccagtca aatcacgatt agaatggcca tatataagca
     1081 aaaaaccctt ggacaatacg tccgatcgac accaaagcaa tctggtacat cacccttctt
     1141 atatacaggg tagccggaat tgtattgact ctatacgaag ccgcaggttt accagcacag
     1201 aggtgcgata gctacacgaa tgtagagtga aggcattaca accgattgct aagtgtcata
     1261 gaggctaagc ttcacggctc aaggagtctg tggaggatac ctgatataag aattgagagt
     1321 tatactccgg aggttatgtt agtacctcaa aaatgtgaag tacaacgaaa ctcgaacttc
     1381 gaatgacaat agcttggctg gctgttagtc aactgaggta caacgtcatc ttgcaattag
     1441 ttatgttctt tgctagatag tgagctgctg taaacttgta gttcttagaa acatgtctga
     1501 agtctagagt cgagacttgt gattggtgta aattagtggg ggtccggagc ggaataacaa
     1561 tcgaggtcag cctcctgggt ctgtcagtaa gctaaccacg aaaggacaag cgtagacgag
     1621 gtcacggggt actggcctag ccggcttcgg ctagcgccca cgaaaatagg gcaacgtcga
     1681 agcgagagac acacacggat tccacgatcg ccgacgacaa gcactcaact ccacgacaac
     1741 ccacgacaac ccttgccgct gcgttcttgg tgaagggctg caggattaga ggtgagtatt
     1801 ttattttctg gctattgtga tctttttccc ccccggtttc cgctggttct atcttgtcga
     1861 gcgagtcggt gcgccttcga tcggtctgtc aaatttccat ccgtccactc gttcacccgg
     1921 tatcgataca agagggaatg gagcattacg acaggggagc agaggcagaa ctctcgatgt
     1981 tgctactttt tttgcttgga acaacttcct tgaaagattc ttttctgaat gcggctggga
     2041 aactgcgaat tggatatcca gcgaaaggtt accgacagac tcacggccat cgataccact
     2101 cagcgggata gaattgaagg cattcggaca ttagatgatc agcttcgctt gtttcgactc
     2161 gtgctaatca attatccctg gacagtcccc cacatagaca gcaaacaatg ccacccaagc
     2221 gcaaaacgct taacgctgcg gctgaggcta acgctcatgc cgacggacac gccgacggaa
     2281 acgccgacgg acacgtggcc aatacggccg cgtcctcgaa taatgcgagg ttcgctgatc
     2341 tcactaacat cgatactccg ggtctgggac ccacaactac gaccctgctc gtggaaccag
     2401 cacgctcaaa gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca gcccgagaga
     2461 aatgcgacgg aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg tcctgcactt
     2521 atcaggcttc gccgaaaaag aggggagttc aaaccggtta tattcgtacg ctggagctcg
     2581 ccctcgcctg gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat aacctcctcg
     2641 tccgtgacgc cggacaagga tcagctctgc tcgttggtaa agattcgccg gctgccgagc
     2701 gactccatgc ccgttgggct actagccgtg tcaataagag cattacccgc ctcctcaggt
     2761 ttgaaagcgg cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg
     2821 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     2881 ttgacatgct ccccgggttc gttatggtga gcaagggcga ggagctgttc accggggtgg
     2941 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     3001 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     3061 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     3121 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3181 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3241 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3301 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3361 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3421 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3481 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3541 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3601 gcatggacga gctgtacaag ccaaagaaga agcggaaggt ctaagcttac ttctttatcg
     3661 gttctctctt acgacttttt gaatggaacg tttccttctt ctcaggcggg cctatctttg
     3721 ggccgaagct cttttccttg tactgtagga cctggttgat aatgattccc aaaaagacat
     3781 ccagcatgtc agttacttgc attcgtcagt ctatacaaaa gcaatggttt agagaaattt
     3841 tgaactttat acatggtttt atttgttgct tcacggccgt accttctgga aatccacggt
     3901 aggagtgtca atttgcgttt ttgataatcc ttccaaggtt cttctcgaag tagttgttct
     3961 ataattgctt cacagctacc atggaacatc ccaagcaccg ctactagaat tcgagctcgg
     4021 agtggatccc ccgggctgca ggaattcgag ctctgtacag tgaccggtga ctctttctgg
     4081 catgcggaga gacggacgga cgcagagaga agggctgagt aataagcgcc actgcgccag
     4141 acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc
     4201 cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg
     4261 agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt
     4321 gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg
     4381 atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc
     4441 gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc
     4501 ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac
     4561 cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc
     4621 tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca
     4681 aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct
     4741 cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt
     4801 tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc
     4861 tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc
     4921 ttgagcagac atcaccatgc ctcaatcctg ggaagaactg gccgctgata agcgcgcccg
     4981 cctcgcaaaa accatccctg atgaatggaa agtccagacg ctgcctgcgg atgacagcgt
     5041 tattgatttc ccaaagaaat cggggatcct ttcagaggcc gaactgaaga tcacagaggc
     5101 ctccgctgca gatcttgtgt ccaagctggc ggccggagag ttgacctcgg tggaagttac
     5161 gctagcattc tgtaaacggg cagcaatcgc ccagcagtta gtagggtccc ctctacctct
     5221 cagggagatg taacaacgcc accttatggg actatcaagc tgacgctggc ttctgtgcag
     5281 acaaactgcg cccacgagtt cttccctgac gccgctctcg cgcaggcaag ggaactcgat
     5341 gaatactacg caaagcacaa gacacccgtt ggtccactcc atggcctccc catctctctc
     5401 aaagaccagc ttcgagtcaa ggtacaccgt tgcccctaag tcgttagatg tccctttttg
     5461 tcagctaaca tatgccacca gggctacgaa acatcaatgg gctacatctc atggctaaac
     5521 aagtacgacg aaggggactc ggttctgaca accatgctcc gcaaagccgg tgccgttttc
     5581 tacgtcaaga cctctgtccc gcagaccctg atggtctgcg agacagtcaa caacatcatc
     5641 gggcgcaccg tcaacccacg caacaagaac tggtcgtgcg gcggcagttc tggtggtgag
     5701 ggtgcgatcg ttgggattcg tggtggcgtc atcggtgtag gaacggatat cggtggctcg
     5761 attcgagtgc cggccgcgtt caacttcctg tacggtctaa ggccgagtca tgggcggctg
     5821 ccgtatgcaa agatggcgaa cagcatggag ggtcaggaga cggtgcacag cgttgtcggg
     5881 ccgattacgc actctgttga gggtgagtcc ttcgcctctt ccttcttttc ctgctctata
     5941 ccaggcctcc actgtcctcc tttcttgctt tttatactat atacgagacg ggcagtcact
     6001 gatgaagtaa tgttagacct ccgcctcttc accaaatccg tcctcggtca ggagccatgg
     6061 aaatacgact ccaaggtcat ccccatgccc tggcgccagt ccgagtcgga cattattgcc
     6121 tccaagatca agaacggcgg gctcaatatc ggctactaca acttcgacgg caatgtcctt
     6181 ccacaccctc ctatcctgcg cggcgtggaa accaccgtcg ccgcactcgc caaagccggt
     6241 cataccgtga ccccgtggac gccatacaag cacgatttcg gccacgatct catctcccat
     6301 atctacgcgg ctgacggcag cgccgacgta atgcgcgata tcagtgcatc cggcgagccg
     6361 gcgattccaa atatcaaaga cctactgaac ccgaacatca aagctgttaa catgaacgag
     6421 ctctgggaca cgcatctcca gaagtggaat taccagatgg agtaccttga gaaatggcgg
     6481 gaggctgaag aaaaggccgg gaaggaactg gacgccatca tcgcgccgat tacgcctacc
     6541 gctgcggtac ggcatgacca gttccggtac tatgggtatg cctctgtgat caacctgctg
     6601 gatttcacga gcgtggttgt tccggttacc tttgcggata agaacatcga taagaagaat
     6661 gagagtttca aggcggttag tgagcttgat gccctcgtgc aggaagagta tgatccggag
     6721 gcgtaccatg gggcaccggt tgcagtgcag gttatcggac ggagactcag tgaagagagg
     6781 acgttggcga ttgcagagga agtggggaag ttgctgggaa atgtggtgac tccataggct
     6841 tctaataagt gtcagatagc aatttgcaca agaaatcaat accagcaact gtaaataagc
     6901 gctgaagtga ccatgccatg ctacgaaaga gcagaaaaaa acctgccgta gaaccgaaga
     6961 gatatgacac gcttccatct ctcaaaggaa gaatcccttc agggttgcgt ttccagtcta
     7021 gacacgtata acggcacaag tgtctctcac caaatgggtt atatctcaaa tgtgatctaa
     7081 ggatggaaag cccagaatat tggctgggtt gatggctgct tcgagtgcag tctcatgctg
     7141 ccacaggtga ctctggatgg ccccatacca ctcaacccat ggtacgagcg gtacgcttta
     7201 cgaattccca tggggagctg cattggtctg ccattgcagg gtatatatgg ctgacctggc
     7261 caatctccat cgagaatctg ggcgactgaa gcactgcccg cagacaagat ggagactttc
     7321 gtctagcacg gtctagggca gatccgatgc cattggctct gtcaactgtc gactacatgt
     7381 atctgcatgt tgcatcggga aatcccacca cagggacagc caagcggccc cgcgacttgg
     7441 cagtgggcaa actacgcccg attctggtgc caagaaccga gaagaatgag acagacccac
     7501 gttgcactct aaccggatgc tatcgactta cggtggctga agattcaaca cgctgcaacg
     7561 agagccaagg tggtccggac attttctacg tgccggttta ccttggaaca tcgccgtcgt
     7621 tgagtgcacg ttgcctactc tctcgtggct tggctgggcc cacgagcccg attgactcga
     7681 cggtgttact tgggtatcta tggccccgtt ttctggcacg gtaatgataa gtacttacta
     7741 atcttcgagc gggggagtgt tgctctgccc gagcatcaac gattggcctg atcgcaccgt
     7801 ctgcaaatgc cacggtgcgg accgactgaa atctcagacc accaaagacc ctccgacttc
     7861 gagatacggt tactaatttt acactggctc cagcggcccc atccagtaag catctgggct
     7921 gcaagcgtat aatgtctcca ggttgtctca gcataaacac cccgcccccg ctcaggcaca
     7981 caggaagaga gctcaggtcg tttccattgc gtccatactc ttcactcatt gtcatctgca
     8041 ggagaacttc ccctgtccct ttgccaagcc ctctcttcgt cgttgtccac gccttcaagt
     8101 tttcaacatt atttttctag acaccaatgg cctcctccga ggacgtcatc aaggagttca
     8161 tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     8221 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
     8281 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt
     8341 acgtgaagca ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca
     8401 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     8461 ccctgcagga cggctccttc atctacaagg tgaagttcat cggcgtgaac ttcccctccg
     8521 acggccccgt aatgcagaag aaaactatgg gctgggaggc ctccaccgag cgcctgtacc
     8581 cccgcgacgg cgtgctgaag ggcgagatcc acaaggccct gaagctgaag gacggcggcc
     8641 actacctggt ggagttcaag tccatctaca tggccaagaa gcccgtgcag ctgcccggct
     8701 actactacgt ggactccaag ctggacatca cctcccacaa cgaggactac accatcgtgg
     8761 agcagtacga gcgcgccgag ggccgccacc acctgttcct gactcacggt atggacgaat
     8821 tgtacaagtc gaagctgtaa gcttgtgctt ctaaggtatg agtcgcaaaa ttgtttttta
     8881 tttttggtct tgagtctaat atgctcgcag ctcttgcgtt gtatatggtc gttgtcgcgt
     8941 attttctgtt gtattaaaag atcaaacgag atcaagggat ggctcgcggg ctgtctctcg
     9001 cactaggagg aagaatgcct gaaaaaggaa ctttgatttt agctgtggaa tagagatggc
     9061 ttgtttgagg acgcttgtcg cttggcgcag ggacttgaat ggcagcttgt ggaaaccgaa
     9121 ggcgagaaaa gtcgacggat actgtacgtg gttctattgc cagtgcggtg gaagcttcct
     9181 tctcatatag ttcaatcctt ctttgaatct gtttgtttca tatttggact gtttcattct
     9241 ctgcttgcgc attctcatct tcgagaacga ctgcagggat tgttggttct gtggagctga
     9301 tgagcgcgcc ttgaccaccc ttgttcttgt tttgctcttt tgttctcatt taacccgttt
     9361 ctcccttcca accctttgac cttgcaacat tgtctcccag cgcgttgcca aagcgaactt
     9421 gatatcagta tagtatgacc aagtagtcta ccaaaataaa ttttagtaca gtattgctag
     9481 ttatacaaga taaattttgt acaacaatga ttatgcagag ctggtattct gtaaggagta
     9541 aattcatgca ggtacagagc gtccacgtca ggcacacgcg ctttagtgct gggtacacat
     9601 tatgctacct ttggggcaag gtgctattct aggtagggta tgcctagcaa tgccatgatc
     9661 ttatgacctc agtaatctga ccttggattt aagatcggag ttctcaaacc ctgcgcgctc
     9721 agagaattcg catgcggaga ctttagacat acctctcgtt gccgacaaat cggcattccg
     9781 tttcggtcac ggacgtaaga gttcaacaaa gccgctcacc tgtcccgttc ggcgcttgag
     9841 tttccacttc aaaagcacaa ccgctccgat atttccattg ccaccgacca acaaaaggtt
     9901 catatcgcgc aatagtcgtc caactctcac tccgcccgca ctcgatggcg aaggatggaa
     9961 gaatatccga gcggcatcgg ataaagagat ctggttcttt tcgtgaagta acgtagggaa
    10021 ccgacggaaa ctgtagagct ataggaattt agtatatcag aagtcaattg agagaagtgt
    10081 atagttagta actcacttgt gccaacaggt atttccaacc gttcttgttc aaatttcgtc
    10141 aagtcaccat ctctatgtgc aaaactggat catacaacag ctcttattgt agctgcatct
    10201 cgctctcaga gatcatccta tcttcgtcgt catccaccaa cacaggtggg agtgctagtt
    10261 gatccatgct gaggtcaagg gacacagacg gaatgggtgt agaacgcctc aaaacaggta
    10321 aggtatcagg atcggttgtc cagggggaat ccaaaccagg ttccaccttg attcagagaa
    10381 taagtatgac gtattcttgg caatgtttaa gcttgagatg agccgatgtc aaactgggag
    10441 acatgatgaa ggaggaaatg ttgctttggt gggggagaca ctataaatac caaagagtga
    10501 atggttgata ccatttgggg aaggagtgtc tatcgcagtc tctgcagacg gcatatcgga
    10561 tagcctgcag gcgcttgtgg aggatgcaca tgtgaccgag ggacacgaag tgatccgttt
    10621 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
    10681 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
    10741 gccagccgcc tttgcgacgc tcaccgggct ggttgccctc gccgctgggc tggcggccgt
    10801 ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac accgcggccg
    10861 ccggcgttgt ggatacctcg cggaaaactt ggccctcact gacagatgag gggcggacgt
    10921 tgacacttga ggggccgact cacccggcgc ggcgttgaca gatgaggggc aggctcgatt
    10981 tcggccggcg acgtggagct ggccagcctc gcaaatcggc gaaaacgcct gattttacgc
    11041 gagtttccca cagatgatgt ggacaagcct ggggataagt gccctgcggt attgacactt
    11101 gaggggcgcg actactgaca gatgaggggc gcgatccttg acacttgagg ggcagagtgc
    11161 tgacagatga ggggcgcacc tattgacatt tgaggggctg tccacaggca gaaaatccag
    11221 catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt aacctgcttt
    11281 taaaccaata tttataaacc ttgtttttaa ccagggctgc gccctgtgcg cgtgaccgcg
    11341 cacgccgaag gggggtgccc ccccttctcg aaccctcccg gcccgctaac gcgggcctcc
    11401 catcccccca ggggctgcgc ccctcggccg cgaacggcct caccccaaaa atggcagcgc
    11461 tggccaattc ccgagtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    11521 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    11581 gtatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg
    11641 taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa
    11701 acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg
    11761 aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg
    11821 aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg
    11881 aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca
    11941 ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct
    12001 tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg
    12061 aagaggacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc
    12121 ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag
    12181 atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg
    12241 acattgcctt ctgcgtccgg tcgctcaggg aggatatcgg ggaagaacag tatgtcgagc
    12301 tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac
    12361 tggatgaatt gttttagctg tcagaccaag tttactcata tatactttag attgatttaa
    12421 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    12481 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    12541 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    12601 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    12661 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    12721 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    12781 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    12841 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    12901 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    12961 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    13021 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    13081 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    13141 ccagcaacgc ggccttttta cggttcctgg cagatcctag atgtggcgca acgatgccgg
    13201 cgacaagcag gagcgcaccg acttcttccg catcaagtgt tttggctctc aggccgaggc
    13261 ccacggcaag tatttgggca aggggtcgct ggtattcgtg cagggcaaga ttcggaatac
    13321 caagtacgag aaggacggcc agacggtcta cgggaccgac ttcattgccg ataaggtgga
    13381 ttatctggac accaaggcac caggcgggtc aaatcaggaa taagggcaca ttgccccggc
    13441 gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg acgtttgacc ggaaggcata
    13501 caggcaagaa ctgatcgacg cggggttttc cgccgaggat gccgaaacca tcgcaagccg
    13561 caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc ggctcgatgg tccagcaagc
    13621 tacggccaag atcgagcgcg acagcgtgca actggctccc cctgccctgc ccgcgccatc
    13681 ggccgccgtg gagcgttcgc gtcgtcttga acaggaggcg gcaggtttgg cgaagtcgat
    13741 gaccatcgac acgcgaggaa ctatgacgac caagaagcga aaaaccgccg gcgaggacct
    13801 ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg aaacacacga agcagcagat
    13861 caaggaaatg cagctttcct tgttcgatat tgcgccgtgg ccggacacga tgcgagcgat
    13921 gccaaacgac acggcccgct ctgccctgtt caccacgcgc aacaagaaaa tcccgcgcga
    13981 ggcgctgcaa aacaaggtca ttttccacgt caacaaggac gtgaagatca cctacaccgg
    14041 cgtcgagctg cgggccgacg atgacgaact ggtgtggcag caggtgttgg agtacgcgaa
    14101 gcgcacccct atcggcgagc cgatcacctt cacgttctac gagctttgcc aggacctggg
    14161 ctggtcgatc aatggccggt attacacgaa ggccgaggaa tgcctgtcgc gcctacaggc
    14221 gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg gaatcggtgt cgctgctgca
    14281 ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt tgccaggtcc tgatcgacga
    14341 ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa ttcatatggg agaagtaccg
    14401 caagctgtcg ccgacggccc gacggatgtt cgactatttc agctcgcacc gggagccgta
    14461 cccgctcaag ctggaaacct tccgcctcat gtgcggatcg gattccaccc gcgtgaagaa
    14521 gtggcgcgag caggtcggcg aagcctgcga agagttgcga ggcagcggcc tggtggaaca
    14581 cgcctgggtc aatgatgacc tggtgcattg caaacgctag ggccttgtgg ggtcagttcc
    14641 ggctgggggt tcagcagccc ctgctcggat ctgttggacc ggacagtagt catggttgat
    14701 gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg
    14761 gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca
    14821 ttgcggacgt ttttaatgta ctggggttga acactct
//
