LOCUS       sequence-306046-       14353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, 
            activating DsRed-SKL with promoter 11xQUAS+CP_pcbC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14353)
  AUTHORS   Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
  TITLE     Synthetic control devices for gene regulation in Penicillium 
            chrysogenum.
  JOURNAL   Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 
            10.1186/s12934-019-1253-3.
  PUBMED    31739777
REFERENCE   2  (bases 1 to 14353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2019-11-18"; volume: "18(1):203. doi"; pages: " 
            10.1186/s12934-019-1253-3"
COMMENT     Sequence Label: pVE2_3_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_11xQUAS
FEATURES             Location/Qualifiers
     source          1..14353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2781..2891
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2949..2970)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3210..3229)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3557..3578
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3621..3641
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4813..4928
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             7623..8297
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(7753..7771)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7803..7823)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8215..8238
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    10131..10155
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      10243..10953
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10974..11078
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11079..11873
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12044..12632
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12533..12552
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12968..14116
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14262..14286
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
       61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
      121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
      181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
      241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
      301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
      361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
      421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
      481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
      541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
      601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
      661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
      721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
      781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
      841 gaattcaagc ttggaggagt tatagacggt ccggcatagg taaggagaga ttagatattt
      901 cgacttcccg attccttagt ctccttagtc tggaaactga gaaaggcctg aactcgttca
      961 atctattagc tctaatcacg tagtctcaac tagaatcctg aacaggcgcc ggaaccagtc
     1021 caaatgcgtg gagtacagca agtccagtca aatcacgatt agaatggcca tatataagca
     1081 aaaaaccctt ggacaatacg tccgatcgac accaaagcaa tctggtacat cacccttctt
     1141 atatacaggg tagccggaat tgtattgact ctatacgaag ccgcaggttt accagcacag
     1201 aggtgcgata gctacacgaa tgtagagtga aggcattaca accgattgct aagtgtcata
     1261 gaggctaagc ttcacggctc aaggagtctg tggaggatac ctgatataag aattgagagt
     1321 tatactccgg aggttatgtt agtacctcaa aaatgtgaag tacaacgaaa ctcgaacttc
     1381 gaatgacaat agcttggctg gctgttagtc aactgaggta caacgtcatc ttgcaattag
     1441 ttatgttctt tgctagatag tgagctgctg taaacttgta gttcttagaa acatgtctga
     1501 agtctagagt cgagacttgt gattggtgta aattagtggg ggtccggagc ggaataacaa
     1561 tcgaggtcag cctcctgggt ctgtcagtaa gctaaccacg aaaggacaag cgtagacgag
     1621 gtcacggggt actggcctag ccggcttcgg ctagcgccca cgaaaatagg gcaacgtcga
     1681 agcgagagac acacacggat tccacgatcg ccgacgacaa gcactcaact ccacgacaac
     1741 ccacgacaac ccttgccgct gcgttcttgg tgaagggctg caggattaga ggtgagtatt
     1801 ttattttctg gctattgtga tctttttccc ccccggtttc cgctggttct atcttgtcga
     1861 gcgagtcggt gcgccttcga tcggtctgtc aaatttccat ccgtccactc gttcacccgg
     1921 tatcgataca agagggaatg gagcattacg acaggggagc agaggcagaa ctctcgatgt
     1981 tgctactttt tttgcttgga acaacttcct tgaaagattc ttttctgaat gcggctggga
     2041 aactgcgaat tggatatcca gcgaaaggtt accgacagac tcacggccat cgataccact
     2101 cagcgggata gaattgaagg cattcggaca ttagatgatc agcttcgctt gtttcgactc
     2161 gtgctaatca attatccctg gacagtcccc cacatagaca gcaaacaatg ccacccaagc
     2221 gcaaaacgct taacgctgcg gctgaggcta acgctcatgc cgacggacac gccgacggaa
     2281 acgccgacgg acacgtggcc aatacggccg cgtcctcgaa taatgcgagg ttcgctgatc
     2341 tcactaacat cgatactccg ggtctgggac ccacaactac gaccctgctc gtggaaccag
     2401 cacgctcaaa gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca gcccgagaga
     2461 aatgcgacgg aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg tcctgcactt
     2521 atcaggcttc gccgaaaaag aggggagttc aaaccggtta tattcgtacg ctggagctcg
     2581 ccctcgcctg gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat aacctcctcg
     2641 tccgtgacgc cggacaagga tcagctctgc tcgttggtaa agattcgccg gctgccgagc
     2701 gactccatgc ccgttgggct actagccgtg tcaataagag cattacccgc ctcctcaggt
     2761 ttgaaagcgg cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg
     2821 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     2881 ttgacatgct ccccgggttc gttatggtga gcaagggcga ggagctgttc accggggtgg
     2941 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     3001 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     3061 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     3121 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3181 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3241 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3301 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3361 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3421 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3481 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3541 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3601 gcatggacga gctgtacaag ccaaagaaga agcggaaggt ctaagcttac ttctttatcg
     3661 gttctctctt acgacttttt gaatggaacg tttccttctt ctcaggcggg cctatctttg
     3721 ggccgaagct cttttccttg tactgtagga cctggttgat aatgattccc aaaaagacat
     3781 ccagcatgtc agttacttgc attcgtcagt ctatacaaaa gcaatggttt agagaaattt
     3841 tgaactttat acatggtttt atttgttgct tcacggccgt accttctgga aatccacggt
     3901 aggagtgtca atttgcgttt ttgataatcc ttccaaggtt cttctcgaag tagttgttct
     3961 ataattgctt cacagctacc atggaacatc ccaagcaccg ctactagaat tcgagctcgg
     4021 agtggatccc ccgggctgca ggaattcgag ctctgtacag tgaccggtga ctctttctgg
     4081 catgcggaga gacggacgga cgcagagaga agggctgagt aataagcgcc actgcgccag
     4141 acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc
     4201 cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg
     4261 agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt
     4321 gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg
     4381 atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc
     4441 gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc
     4501 ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac
     4561 cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc
     4621 tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca
     4681 aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct
     4741 cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt
     4801 tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc
     4861 tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc
     4921 ttgagcagac atcaccatgc ctcaatcctg ggaagaactg gccgctgata agcgcgcccg
     4981 cctcgcaaaa accatccctg atgaatggaa agtccagacg ctgcctgcgg atgacagcgt
     5041 tattgatttc ccaaagaaat cggggatcct ttcagaggcc gaactgaaga tcacagaggc
     5101 ctccgctgca gatcttgtgt ccaagctggc ggccggagag ttgacctcgg tggaagttac
     5161 gctagcattc tgtaaacggg cagcaatcgc ccagcagtta gtagggtccc ctctacctct
     5221 cagggagatg taacaacgcc accttatggg actatcaagc tgacgctggc ttctgtgcag
     5281 acaaactgcg cccacgagtt cttccctgac gccgctctcg cgcaggcaag ggaactcgat
     5341 gaatactacg caaagcacaa gacacccgtt ggtccactcc atggcctccc catctctctc
     5401 aaagaccagc ttcgagtcaa ggtacaccgt tgcccctaag tcgttagatg tccctttttg
     5461 tcagctaaca tatgccacca gggctacgaa acatcaatgg gctacatctc atggctaaac
     5521 aagtacgacg aaggggactc ggttctgaca accatgctcc gcaaagccgg tgccgttttc
     5581 tacgtcaaga cctctgtccc gcagaccctg atggtctgcg agacagtcaa caacatcatc
     5641 gggcgcaccg tcaacccacg caacaagaac tggtcgtgcg gcggcagttc tggtggtgag
     5701 ggtgcgatcg ttgggattcg tggtggcgtc atcggtgtag gaacggatat cggtggctcg
     5761 attcgagtgc cggccgcgtt caacttcctg tacggtctaa ggccgagtca tgggcggctg
     5821 ccgtatgcaa agatggcgaa cagcatggag ggtcaggaga cggtgcacag cgttgtcggg
     5881 ccgattacgc actctgttga gggtgagtcc ttcgcctctt ccttcttttc ctgctctata
     5941 ccaggcctcc actgtcctcc tttcttgctt tttatactat atacgagacg ggcagtcact
     6001 gatgaagtaa tgttagacct ccgcctcttc accaaatccg tcctcggtca ggagccatgg
     6061 aaatacgact ccaaggtcat ccccatgccc tggcgccagt ccgagtcgga cattattgcc
     6121 tccaagatca agaacggcgg gctcaatatc ggctactaca acttcgacgg caatgtcctt
     6181 ccacaccctc ctatcctgcg cggcgtggaa accaccgtcg ccgcactcgc caaagccggt
     6241 cataccgtga ccccgtggac gccatacaag cacgatttcg gccacgatct catctcccat
     6301 atctacgcgg ctgacggcag cgccgacgta atgcgcgata tcagtgcatc cggcgagccg
     6361 gcgattccaa atatcaaaga cctactgaac ccgaacatca aagctgttaa catgaacgag
     6421 ctctgggaca cgcatctcca gaagtggaat taccagatgg agtaccttga gaaatggcgg
     6481 gaggctgaag aaaaggccgg gaaggaactg gacgccatca tcgcgccgat tacgcctacc
     6541 gctgcggtac ggcatgacca gttccggtac tatgggtatg cctctgtgat caacctgctg
     6601 gatttcacga gcgtggttgt tccggttacc tttgcggata agaacatcga taagaagaat
     6661 gagagtttca aggcggttag tgagcttgat gccctcgtgc aggaagagta tgatccggag
     6721 gcgtaccatg gggcaccggt tgcagtgcag gttatcggac ggagactcag tgaagagagg
     6781 acgttggcga ttgcagagga agtggggaag ttgctgggaa atgtggtgac tccataggct
     6841 tctaataagt gtcagatagc aatttgcaca agaaatcaat accagcaact gtaaataagc
     6901 gctgaagtga ccatgccatg ctacgaaaga gcagaaaaaa acctgccgta gaaccgaaga
     6961 gatatgacac gcttccatct ctcaaaggaa gaatcccttc agggttgcgt ttccagtcta
     7021 gacacgtata acggcacaag tgtctctcac caaatgggtt atatctcaaa tgtgatctaa
     7081 ggatggaaag cccagaatat tggctgggtt gatggctgct tcgagtgcag tctcatgctg
     7141 ccacaggtga ctctggatgg ccccatacca ctcaacccat ggtacgagcg gtacgcttta
     7201 cgaattccca tggggagggg taatcgatta tcctcggata aacacttatc ccagggtaat
     7261 cacttatccc tggataaaca attatcctcg ggtaatcgct tatcctacgg ggtaatcgct
     7321 tatccttgcg ggtaatcgct tatcctcgga taaacaatta tcccagggta atcacttatc
     7381 cctggataaa caattatcct cgggtaatcg cttatcccca tcgtataatg tctccaggtt
     7441 gtctcagcat aaacaccccg cccccgctca ggcacacagg aagagagctc aggtcgtttc
     7501 cattgcgtcc atactcttca ctcattgtca tctgcaggag aacttcccct gtccctttgc
     7561 caagccctct cttcgtcgtt gtccacgcct tcaagttttc accattattt ttctagacac
     7621 caatggcctc ctccgaggac gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg
     7681 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     7741 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     7801 tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc
     7861 ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     7921 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc tccttcatct
     7981 acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtaatg cagaagaaaa
     8041 ctatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg
     8101 agatccacaa ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagtcca
     8161 tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac tccaagctgg
     8221 acatcacctc ccacaacgag gactacacca tcgtggagca gtacgagcgc gccgagggcc
     8281 gccaccacct gttcctgact cacggtatgg acgaattgta caagtcgaag ctgtaagctt
     8341 gtgcttctaa ggtatgagtc gcaaaattgt tttttatttt tggtcttgag tctaatatgc
     8401 tcgcagctct tgcgttgtat atggtcgttg tcgcgtattt tctgttgtat taaaagatca
     8461 aacgagatca agggatggct cgcgggctgt ctctcgcact aggaggaaga atgcctgaaa
     8521 aaggaacttt gattttagct gtggaataga gatggcttgt ttgaggacgc ttgtcgcttg
     8581 gcgcagggac ttgaatggca gcttgtggaa accgaaggcg agaaaagtcg acggatactg
     8641 tacgtggttc tattgccagt gcggtggaag cttccttctc atatagttca atccttcttt
     8701 gaatctgttt gtttcatatt tggactgttt cattctctgc ttgcgcattc tcatcttcga
     8761 gaacgactgc agggattgtt ggttctgtgg agctgatgag cgcgccttga ccacccttgt
     8821 tcttgttttg ctcttttgtt ctcatttaac ccgtttctcc cttccaaccc tttgaccttg
     8881 caacattgtc tcccagcgcg ttgccaaagc gaacttgata tcagtatagt atgaccaagt
     8941 agtctaccaa aataaatttt agtacagtat tgctagttat acaagataaa ttttgtacaa
     9001 caatgattat gcagagctgg tattctgtaa ggagtaaatt catgcaggta cagagcgtcc
     9061 acgtcaggca cacgcgcttt agtgctgggt acacattatg ctacctttgg ggcaaggtgc
     9121 tattctaggt agggtatgcc tagcaatgcc atgatcttat gacctcagta atctgacctt
     9181 ggatttaaga tcggagttct caaaccctgc gcgctcagag aattcgcatg cggagacttt
     9241 agacatacct ctcgttgccg acaaatcggc attccgtttc ggtcacggac gtaagagttc
     9301 aacaaagccg ctcacctgtc ccgttcggcg cttgagtttc cacttcaaaa gcacaaccgc
     9361 tccgatattt ccattgccac cgaccaacaa aaggttcata tcgcgcaata gtcgtccaac
     9421 tctcactccg cccgcactcg atggcgaagg atggaagaat atccgagcgg catcggataa
     9481 agagatctgg ttcttttcgt gaagtaacgt agggaaccga cggaaactgt agagctatag
     9541 gaatttagta tatcagaagt caattgagag aagtgtatag ttagtaactc acttgtgcca
     9601 acaggtattt ccaaccgttc ttgttcaaat ttcgtcaagt caccatctct atgtgcaaaa
     9661 ctggatcata caacagctct tattgtagct gcatctcgct ctcagagatc atcctatctt
     9721 cgtcgtcatc caccaacaca ggtgggagtg ctagttgatc catgctgagg tcaagggaca
     9781 cagacggaat gggtgtagaa cgcctcaaaa caggtaaggt atcaggatcg gttgtccagg
     9841 gggaatccaa accaggttcc accttgattc agagaataag tatgacgtat tcttggcaat
     9901 gtttaagctt gagatgagcc gatgtcaaac tgggagacat gatgaaggag gaaatgttgc
     9961 tttggtgggg gagacactat aaataccaaa gagtgaatgg ttgataccat ttggggaagg
    10021 agtgtctatc gcagtctctg cagacggcat atcggatagc ctgcaggcgc ttgtggagga
    10081 tgcacatgtg accgagggac acgaagtgat ccgtttaaac tatcagtgtt tgacaggata
    10141 tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg
    10201 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcca gccgcctttg cgacgctcac
    10261 cgggctggtt gccctcgccg ctgggctggc ggccgtctat ggccctgcaa acgcgccaga
    10321 aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg cgttgtggat acctcgcgga
    10381 aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc
    10441 cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc
    10501 agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac
    10561 aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg
    10621 aggggcgcga tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt
    10681 gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt
    10741 tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt
    10801 ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc
    10861 ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc cccccagggg ctgcgcccct
    10921 cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc caattcccga gtgcgcggaa
    10981 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    11041 cctgataaat gcttcaataa tattgaaaaa ggaagagtat ggctaaaatg agaatatcac
    11101 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
    11161 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
    11221 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
    11281 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
    11341 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
    11401 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
    11461 cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga
    11521 ataacgatct ggccgatgtg gattgcgaaa actgggaaga ggacactcca tttaaagatc
    11581 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
    11641 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
    11701 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcgc
    11761 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
    11821 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagctgtcag
    11881 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    11941 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    12001 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    12061 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    12121 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    12181 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    12241 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    12301 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    12361 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    12421 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    12481 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    12541 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    12601 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    12661 tcctggcaga tcctagatgt ggcgcaacga tgccggcgac aagcaggagc gcaccgactt
    12721 cttccgcatc aagtgttttg gctctcaggc cgaggcccac ggcaagtatt tgggcaaggg
    12781 gtcgctggta ttcgtgcagg gcaagattcg gaataccaag tacgagaagg acggccagac
    12841 ggtctacggg accgacttca ttgccgataa ggtggattat ctggacacca aggcaccagg
    12901 cgggtcaaat caggaataag ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg
    12961 agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga tcgacgcggg
    13021 gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga
    13081 aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg agcgcgacag
    13141 cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg
    13201 tcttgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc gaggaactat
    13261 gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca gcgaggccaa
    13321 gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc tttccttgtt
    13381 cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg cccgctctgc
    13441 cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt
    13501 ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg ccgacgatga
    13561 cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg gcgagccgat
    13621 caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg gccggtatta
    13681 cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga
    13741 ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg
    13801 caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg
    13861 cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg
    13921 gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg aaaccttccg
    13981 cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc
    14041 ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg atgacctggt
    14101 gcattgcaaa cgctagggcc ttgtggggtc agttccggct gggggttcag cagcccctgc
    14161 tcggatctgt tggaccggac agtagtcatg gttgatgggc tgcctgtatc gagtggtgat
    14221 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt
    14281 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactgg
    14341 ggttgaacac tct
//
