LOCUS       sequence-306049-       13574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, 
            activating DsRed-SKL with promoter 5xQUAS+CP_nirA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13574)
  AUTHORS   Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
  TITLE     Synthetic control devices for gene regulation in Penicillium 
            chrysogenum.
  JOURNAL   Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 
            10.1186/s12934-019-1253-3.
  PUBMED    31739777
REFERENCE   2  (bases 1 to 13574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2019-11-18"; volume: "18(1):203. doi"; pages: " 
            10.1186/s12934-019-1253-3"
COMMENT     Sequence Label: pLM2_41_pgndA_QfDBD_VP16AD_cp_nirA_DsRed_5xQUAS
FEATURES             Location/Qualifiers
     source          1..13574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2222..2332
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             join(2345..2347,2348..2350,2351..3061)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2345..2347,2348..2350,2351..3061)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2390..2411)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2651..2670)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2998..3019
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3062..3082
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4254..4369
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             6844..7518
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(6974..6992)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7024..7044)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     7436..7459
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    9352..9376
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      9464..10174
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10195..10299
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10300..11094
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11265..11853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11754..11773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12189..13337
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    13483..13507
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
       61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
      121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
      181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
      241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
      301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
      361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
      421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
      481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
      541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
      601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
      661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
      721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
      781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
      841 gaattcaagc ttggagtctt gcgttacggg cgtattttgc tgcggccggt ggtgcccctc
      901 catgccccgc catctttcaa agctcctggc gacgccgtca tctccgaaca ttctcccccc
      961 aaaggaatca attggcaatt ggagtctagt aaagtggtgt ttgtcatcag taaggagttg
     1021 gtgaaactac aatcttccat catgaagaga agggatattt ttggggttgt attttacgat
     1081 gaaggtactg gaaatggtgg gggtttttat agcagtagac agtcagtcag taagtagtat
     1141 gcttgttgta ttacccaaac cagatcaatc caaagaaagc ctgacagaca gccatcaata
     1201 gatactactt cgtactatag ttacccacct aaccatatta ctcaaaaagc atctatctat
     1261 ccgcgggctt ccatgcatgt cccggtagca aactcctccc accggtgtag tactctttgg
     1321 ttagtagtct tgttcaccgg aggactctgc tcctctcctg ctcaggtgct gccccgccct
     1381 ccgtcccacc atgacggaag agatgctccg taagccgtcc agttgcaacg aatcctgctc
     1441 tgacatcttc gaacgccttc tccctttcgc tcgcttctct gcctctttcc tctcttccct
     1501 ttccttcccc tccaaactaa accttcctcc ttttctccat catcctctag gcagttggtt
     1561 cttcctgact gtacatatat ccaccacctc ccccctctat tcttccacct cttccatatc
     1621 tccttctcca gagttcatac cccccacaat gccacccaag cgcaaaacgc ttaacgctgc
     1681 ggctgaggct aacgctcatg ccgacggaca cgccgacgga aacgccgacg gacacgtggc
     1741 caatacggcc gcgtcctcga ataatgcgag gttcgctgat ctcactaaca tcgatactcc
     1801 gggtctggga cccacaacta cgaccctgct cgtggaacca gcacgctcaa agcgtcaacg
     1861 agtgtcccgc gcatgcgacc agtgccgtgc agcccgagag aaatgcgacg gaatacagcc
     1921 tgcgtgtttc ccgtgcgttt cccagggaag gtcctgcact tatcaggctt cgccgaaaaa
     1981 gaggggagtt caaaccggtt atattcgtac gctggagctc gccctcgcct ggatgtttga
     2041 aaatgtcgcg cgttccgaag atgccttgca taacctcctc gtccgtgacg ccggacaagg
     2101 atcagctctg ctcgttggta aagattcgcc ggctgccgag cgactccatg cccgttgggc
     2161 tactagccgt gtcaataaga gcattacccg cctcctcagg tttgaaagcg gcgggccggc
     2221 cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga
     2281 ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggtt
     2341 cgttatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2401 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2461 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2521 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     2581 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2641 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2701 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2761 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     2821 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2881 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2941 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3001 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3061 gccaaagaag aagcggaagg tctaagctta cttctttatc ggttctctct tacgactttt
     3121 tgaatggaac gtttccttct tctcaggcgg gcctatcttt gggccgaagc tcttttcctt
     3181 gtactgtagg acctggttga taatgattcc caaaaagaca tccagcatgt cagttacttg
     3241 cattcgtcag tctatacaaa agcaatggtt tagagaaatt ttgaacttta tacatggttt
     3301 tatttgttgc ttcacggccg taccttctgg aaatccacgg taggagtgtc aatttgcgtt
     3361 tttgataatc cttccaaggt tcttctcgaa gtagttgttc tataattgct tcacagctac
     3421 catggaacat cccaagcacc gctactagaa ttcgagctcg gagtggatcc cccgggctgc
     3481 aggaattcga gctctgtaca gtgaccggtg actctttctg gcatgcggag agacggacgg
     3541 acgcagagag aagggctgag taataagcgc cactgcgcca gacagctctg gcggctctga
     3601 ggtgcagtgg atgattatta atccgggacc ggccgcccct ccgccccgaa gtggaaaggc
     3661 tggtgtgccc ctcgttgacc aagaatctat tgcatcatcg gagaatatgg agcttcatcg
     3721 aatcaccggc agtaagcgaa ggagaatgtg aagccagggg tgtatagccg tcggcgaaat
     3781 agcatgccat taacctaggt acagaagtcc aattgcttcc gatctggtaa aagattcacg
     3841 agatagtacc ttctccgaag taggtagagc gagtacccgg cgcgtaagct ccctaattgg
     3901 cccatccggc atctgtaggg cgtccaaata tcgtgcctct cctgctttgc ccggtgtatg
     3961 aaaccggaaa ggccgctcag gagctggcca gcggcgcaga ccgggaacac aagctggcag
     4021 tcgacccatc cggtgctctg cactcgacct gctgaggtcc ctcagtccct ggtaggcagc
     4081 tttgccccgt ctgtccgccc ggtgtgtcgg cggggttgac aaggtcgttg cgtcagtcca
     4141 acatttgttg ccatattttc ctgctctccc caccagctgc tcttttcttt tctctttctt
     4201 ttcccatctt cagtatattc atcttcccat ccaagaacct ttatttcccc taagtaagta
     4261 ctttgctaca tccatactcc atccttccca tcccttattc ctttgaacct ttcagttcga
     4321 gctttcccac ttcatcgcag cttgactaac agctaccccg cttgagcaga catcaccatg
     4381 cctcaatcct gggaagaact ggccgctgat aagcgcgccc gcctcgcaaa aaccatccct
     4441 gatgaatgga aagtccagac gctgcctgcg gatgacagcg ttattgattt cccaaagaaa
     4501 tcggggatcc tttcagaggc cgaactgaag atcacagagg cctccgctgc agatcttgtg
     4561 tccaagctgg cggccggaga gttgacctcg gtggaagtta cgctagcatt ctgtaaacgg
     4621 gcagcaatcg cccagcagtt agtagggtcc cctctacctc tcagggagat gtaacaacgc
     4681 caccttatgg gactatcaag ctgacgctgg cttctgtgca gacaaactgc gcccacgagt
     4741 tcttccctga cgccgctctc gcgcaggcaa gggaactcga tgaatactac gcaaagcaca
     4801 agacacccgt tggtccactc catggcctcc ccatctctct caaagaccag cttcgagtca
     4861 aggtacaccg ttgcccctaa gtcgttagat gtcccttttt gtcagctaac atatgccacc
     4921 agggctacga aacatcaatg ggctacatct catggctaaa caagtacgac gaaggggact
     4981 cggttctgac aaccatgctc cgcaaagccg gtgccgtttt ctacgtcaag acctctgtcc
     5041 cgcagaccct gatggtctgc gagacagtca acaacatcat cgggcgcacc gtcaacccac
     5101 gcaacaagaa ctggtcgtgc ggcggcagtt ctggtggtga gggtgcgatc gttgggattc
     5161 gtggtggcgt catcggtgta ggaacggata tcggtggctc gattcgagtg ccggccgcgt
     5221 tcaacttcct gtacggtcta aggccgagtc atgggcggct gccgtatgca aagatggcga
     5281 acagcatgga gggtcaggag acggtgcaca gcgttgtcgg gccgattacg cactctgttg
     5341 agggtgagtc cttcgcctct tccttctttt cctgctctat accaggcctc cactgtcctc
     5401 ctttcttgct ttttatacta tatacgagac gggcagtcac tgatgaagta atgttagacc
     5461 tccgcctctt caccaaatcc gtcctcggtc aggagccatg gaaatacgac tccaaggtca
     5521 tccccatgcc ctggcgccag tccgagtcgg acattattgc ctccaagatc aagaacggcg
     5581 ggctcaatat cggctactac aacttcgacg gcaatgtcct tccacaccct cctatcctgc
     5641 gcggcgtgga aaccaccgtc gccgcactcg ccaaagccgg tcacaccgtg accccgtgga
     5701 cgccatacaa gcacgatttc ggccacgatc tcatctccca tatctacgcg gctgacggca
     5761 gcgccgacgt aatgcgcgat atcagtgcat ccggcgagcc ggcgattcca aatatcaaag
     5821 acctactgaa cccgaacatc aaagctgtta acatgaacga gctctgggac acgcatctcc
     5881 agaagtggaa ttaccagatg gagtaccttg agaaatggcg ggaggctgaa gaaaaggccg
     5941 ggaaggaact ggacgccatc atcgcgccga ttacgcctac cgctgcggta cggcatgacc
     6001 agttccggta ctatgggtat gcctctgtga tcaacctgct ggatttcacg agcgtggttg
     6061 ttccggttac ctttgcggat aagaacatcg ataagaagaa tgagagtttc aaggcggtta
     6121 gtgagcttga tgccctcgtg caggaagagt atgatccgga ggcgtaccat ggggcaccgg
     6181 ttgcagtgca ggttatcgga cggagactca gtgaagagag gacgttggcg attgcagagg
     6241 aagtggggaa gttgctggga aatgtggtga ctccataggc ttctaataag tgtcagatag
     6301 caatttgcac aagaaatcaa taccagcaac tgtaaataag cgctgaagtg accatgccat
     6361 gctacgaaag agcagaaaaa aacctgccgt agaaccgaag agatatgaca cgcttccatc
     6421 tctcaaagga agaatccctt cagggttgcg tttccagtct agacacgtat aacggcacaa
     6481 gtgtctctca ccaaatgggt tatatctcaa atgtgatcta aggatggaaa gcccagaata
     6541 ttggctgggt tgatggctgc ttcgagtgca gtctcatgct gccacaggtg actctggatg
     6601 gccccatacc actcaaccca tggtacgagc ggtacgcttt acgaattccc atggggaggg
     6661 gtaatcgatt atcctcggat aaacacttat cccagggtaa tcacttatcc ctggataaac
     6721 aattatcctc gggtaatcgc ttatccccat cagggaaaca cgccgagcgt cctcctccga
     6781 taagcatgcg ctgtcttggt ctgtcgctgt caccgaacat atctgtctgg gcttgattta
     6841 ccaatggcct cctccgagga cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag
     6901 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     6961 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     7021 atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc
     7081 cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     7141 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg ctccttcatc
     7201 tacaaggtga agttcatcgg cgtgaacttc ccctccgacg gccccgtaat gcagaagaaa
     7261 actatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     7321 gagatccaca aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagtcc
     7381 atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga ctccaagctg
     7441 gacatcacct cccacaacga ggactacacc atcgtggagc agtacgagcg cgccgagggc
     7501 cgccaccacc tgttcctgac tcacggtatg gacgaattgt acaagtcgaa gctgtaagct
     7561 tgtgcttcta aggtatgagt cgcaaaattg ttttttattt ttggtcttga gtctaatatg
     7621 ctcgcagctc ttgcgttgta tatggtcgtt gtcgcgtatt ttctgttgta ttaaaagatc
     7681 aaacgagatc aagggatggc tcgcgggctg tctctcgcac taggaggaag aatgcctgaa
     7741 aaaggaactt tgattttagc tgtggaatag agatggcttg tttgaggacg cttgtcgctt
     7801 ggcgcaggga cttgaatggc agcttgtgga aaccgaaggc gagaaaagtc gacggatact
     7861 gtacgtggtt ctattgccag tgcggtggaa gcttccttct catatagttc aatccttctt
     7921 tgaatctgtt tgtttcatat ttggactgtt tcattctctg cttgcgcatt ctcatcttcg
     7981 agaacgactg cagggattgt tggttctgtg gagctgatga gcgcgccttg accacccttg
     8041 ttcttgtttt gctcttttgt tctcatttaa cccgtttctc ccttccaacc ctttgacctt
     8101 gcaacattgt ctcccagcgc gttgccaaag cgaacttgat atcagtatag tatgaccaag
     8161 tagtctacca aaataaattt tagtacagta ttgctagtta tacaagataa attttgtaca
     8221 acaatgatta tgcagagctg gtattctgta aggagtaaat tcatgcaggt acagagcgtc
     8281 cacgtcaggc acacgcgctt tagtgctggg tacacattat gctacctttg gggcaaggtg
     8341 ctattctagg tagggtatgc ctagcaatgc catgatctta tgacctcagt aatctgacct
     8401 tggatttaag atcggagttc tcaaaccctg cgcgctcaga gaattcgcat gcggagactt
     8461 tagacatacc tctcgttgcc gacaaatcgg cattccgttt cggtcacgga cgtaagagtt
     8521 caacaaagcc gctcacctgt cccgttcggc gcttgagttt ccacttcaaa agcacaaccg
     8581 ctccgatatt tccattgcca ccgaccaaca aaaggttcat atcgcgcaat agtcgtccaa
     8641 ctctcactcc gcccgcactc gatggcgaag gatggaagaa tatccgagcg gcatcggata
     8701 aagagatctg gttcttttcg tgaagtaacg tagggaaccg acggaaactg tagagctata
     8761 ggaatttagt atatcagaag tcaattgaga gaagtgtata gttagtaact cacttgtgcc
     8821 aacaggtatt tccaaccgtt cttgttcaaa tttcgtcaag tcaccatctc tatgtgcaaa
     8881 actggatcat acaacagctc ttattgtagc tgcatctcgc tctcagagat catcctatct
     8941 tcgtcgtcat ccaccaacac aggtgggagt gctagttgat ccatgctgag gtcaagggac
     9001 acagacggaa tgggtgtaga acgcctcaaa acaggtaagg tatcaggatc ggttgtccag
     9061 ggggaatcca aaccaggttc caccttgatt cagagaataa gtatgacgta ttcttggcaa
     9121 tgtttaagct tgagatgagc cgatgtcaaa ctgggagaca tgatgaagga ggaaatgttg
     9181 ctttggtggg ggagacacta taaataccaa agagtgaatg gttgatacca tttggggaag
     9241 gagtgtctat cgcagtctct gcagacggca tatcggatag cctgcaggcg cttgtggagg
     9301 atgcacatgt gaccgaggga cacgaagtga tccgtttaaa ctatcagtgt ttgacaggat
     9361 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
     9421 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgcc agccgccttt gcgacgctca
     9481 ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag
     9541 aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg
     9601 aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac
     9661 ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc
     9721 cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga
     9781 caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat
     9841 gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat
     9901 tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt
     9961 ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg
    10021 tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc
    10081 cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc
    10141 tcggccgcga acggcctcac cccaaaaatg gcagcgctgg ccaattcccg agtgcgcgga
    10201 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
    10261 ccctgataaa tgcttcaata atattgaaaa aggaagagta tggctaaaat gagaatatca
    10321 ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct
    10381 cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac
    10441 agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg
    10501 gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat
    10561 ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc
    10621 cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata
    10681 tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg
    10741 aataacgatc tggccgatgt ggattgcgaa aactgggaag aggacactcc atttaaagat
    10801 ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc
    10861 cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt
    10921 gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg
    10981 ctcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc
    11041 aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt ttagctgtca
    11101 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    11161 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    11221 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    11281 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    11341 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    11401 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    11461 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    11521 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    11581 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    11641 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    11701 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    11761 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    11821 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    11881 ttcctggcag atcctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact
    11941 tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg
    12001 ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga
    12061 cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag
    12121 gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag
    12181 gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg
    12241 ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg
    12301 aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca
    12361 gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc
    12421 gtcttgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta
    12481 tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca
    12541 agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt
    12601 tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg
    12661 ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt
    12721 tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg
    12781 acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga
    12841 tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt
    12901 acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg
    12961 accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg
    13021 gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg
    13081 gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac
    13141 ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc
    13201 gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag
    13261 cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg
    13321 tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagcccctg
    13381 ctcggatctg ttggaccgga cagtagtcat ggttgatggg ctgcctgtat cgagtggtga
    13441 ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg
    13501 tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg
    13561 gggttgaaca ctct
//
