LOCUS       sequence-306059-       14087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, 
            activating DsRed-SKL with promoter CP_ura3 (no QUAS control).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14087)
  AUTHORS   Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
  TITLE     Synthetic control devices for gene regulation in Penicillium 
            chrysogenum.
  JOURNAL   Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 
            10.1186/s12934-019-1253-3.
  PUBMED    31739777
REFERENCE   2  (bases 1 to 14087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2019-11-18"; volume: "18(1):203. doi"; pages: " 
            10.1186/s12934-019-1253-3"
COMMENT     Sequence Label: pLM2_31_p40S_QfDBD_VP16AD_cp_ura3_DsRed_0xQUAS
FEATURES             Location/Qualifiers
     source          1..14087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2781..2891
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2949..2970)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3210..3229)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3557..3578
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3621..3641
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4813..4928
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             7357..8031
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(7487..7505)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7537..7557)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     7949..7972
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    9865..9889
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      9977..10687
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10708..10812
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10813..11607
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11778..12366
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12267..12286
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12702..13850
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    13996..14020
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
       61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
      121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
      181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
      241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
      301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
      361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
      421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
      481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
      541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
      601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
      661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
      721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
      781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
      841 gaattcaagc ttggaggagt tatagacggt ccggcatagg taaggagaga ttagatattt
      901 cgacttcccg attccttagt ctccttagtc tggaaactga gaaaggcctg aactcgttca
      961 atctattagc tctaatcacg tagtctcaac tagaatcctg aacaggcgcc ggaaccagtc
     1021 caaatgcgtg gagtacagca agtccagtca aatcacgatt agaatggcca tatataagca
     1081 aaaaaccctt ggacaatacg tccgatcgac accaaagcaa tctggtacat cacccttctt
     1141 atatacaggg tagccggaat tgtattgact ctatacgaag ccgcaggttt accagcacag
     1201 aggtgcgata gctacacgaa tgtagagtga aggcattaca accgattgct aagtgtcata
     1261 gaggctaagc ttcacggctc aaggagtctg tggaggatac ctgatataag aattgagagt
     1321 tatactccgg aggttatgtt agtacctcaa aaatgtgaag tacaacgaaa ctcgaacttc
     1381 gaatgacaat agcttggctg gctgttagtc aactgaggta caacgtcatc ttgcaattag
     1441 ttatgttctt tgctagatag tgagctgctg taaacttgta gttcttagaa acatgtctga
     1501 agtctagagt cgagacttgt gattggtgta aattagtggg ggtccggagc ggaataacaa
     1561 tcgaggtcag cctcctgggt ctgtcagtaa gctaaccacg aaaggacaag cgtagacgag
     1621 gtcacggggt actggcctag ccggcttcgg ctagcgccca cgaaaatagg gcaacgtcga
     1681 agcgagagac acacacggat tccacgatcg ccgacgacaa gcactcaact ccacgacaac
     1741 ccacgacaac ccttgccgct gcgttcttgg tgaagggctg caggattaga ggtgagtatt
     1801 ttattttctg gctattgtga tctttttccc ccccggtttc cgctggttct atcttgtcga
     1861 gcgagtcggt gcgccttcga tcggtctgtc aaatttccat ccgtccactc gttcacccgg
     1921 tatcgataca agagggaatg gagcattacg acaggggagc agaggcagaa ctctcgatgt
     1981 tgctactttt tttgcttgga acaacttcct tgaaagattc ttttctgaat gcggctggga
     2041 aactgcgaat tggatatcca gcgaaaggtt accgacagac tcacggccat cgataccact
     2101 cagcgggata gaattgaagg cattcggaca ttagatgatc agcttcgctt gtttcgactc
     2161 gtgctaatca attatccctg gacagtcccc cacatagaca gcaaacaatg ccacccaagc
     2221 gcaaaacgct taacgctgcg gctgaggcta acgctcatgc cgacggacac gccgacggaa
     2281 acgccgacgg acacgtggcc aatacggccg cgtcctcgaa taatgcgagg ttcgctgatc
     2341 tcactaacat cgatactccg ggtctgggac ccacaactac gaccctgctc gtggaaccag
     2401 cacgctcaaa gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca gcccgagaga
     2461 aatgcgacgg aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg tcctgcactt
     2521 atcaggcttc gccgaaaaag aggggagttc aaaccggtta tattcgtacg ctggagctcg
     2581 ccctcgcctg gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat aacctcctcg
     2641 tccgtgacgc cggacaagga tcagctctgc tcgttggtaa agattcgccg gctgccgagc
     2701 gactccatgc ccgttgggct actagccgtg tcaataagag cattacccgc ctcctcaggt
     2761 ttgaaagcgg cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg
     2821 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     2881 ttgacatgct ccccgggttc gttatggtga gcaagggcga ggagctgttc accggggtgg
     2941 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     3001 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     3061 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     3121 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3181 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3241 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3301 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3361 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3421 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3481 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3541 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3601 gcatggacga gctgtacaag ccaaagaaga agcggaaggt ctaagcttac ttctttatcg
     3661 gttctctctt acgacttttt gaatggaacg tttccttctt ctcaggcggg cctatctttg
     3721 ggccgaagct cttttccttg tactgtagga cctggttgat aatgattccc aaaaagacat
     3781 ccagcatgtc agttacttgc attcgtcagt ctatacaaaa gcaatggttt agagaaattt
     3841 tgaactttat acatggtttt atttgttgct tcacggccgt accttctgga aatccacggt
     3901 aggagtgtca atttgcgttt ttgataatcc ttccaaggtt cttctcgaag tagttgttct
     3961 ataattgctt cacagctacc atggaacatc ccaagcaccg ctactagaat tcgagctcgg
     4021 agtggatccc ccgggctgca ggaattcgag ctctgtacag tgaccggtga ctctttctgg
     4081 catgcggaga gacggacgga cgcagagaga agggctgagt aataagcgcc actgcgccag
     4141 acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc
     4201 cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg
     4261 agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt
     4321 gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg
     4381 atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc
     4441 gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc
     4501 ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac
     4561 cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc
     4621 tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca
     4681 aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct
     4741 cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt
     4801 tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc
     4861 tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc
     4921 ttgagcagac atcaccatgc ctcaatcctg ggaagaactg gccgctgata agcgcgcccg
     4981 cctcgcaaaa accatccctg atgaatggaa agtccagacg ctgcctgcgg atgacagcgt
     5041 tattgatttc ccaaagaaat cggggatcct ttcagaggcc gaactgaaga tcacagaggc
     5101 ctccgctgca gatcttgtgt ccaagctggc ggccggagag ttgacctcgg tggaagttac
     5161 gctagcattc tgtaaacggg cagcaatcgc ccagcagtta gtagggtccc ctctacctct
     5221 cagggagatg taacaacgcc accttatggg actatcaagc tgacgctggc ttctgtgcag
     5281 acaaactgcg cccacgagtt cttccctgac gccgctctcg cgcaggcaag ggaactcgat
     5341 gaatactacg caaagcacaa gacacccgtt ggtccactcc atggcctccc catctctctc
     5401 aaagaccagc ttcgagtcaa ggtacaccgt tgcccctaag tcgttagatg tccctttttg
     5461 tcagctaaca tatgccacca gggctacgaa acatcaatgg gctacatctc atggctaaac
     5521 aagtacgacg aaggggactc ggttctgaca accatgctcc gcaaagccgg tgccgttttc
     5581 tacgtcaaga cctctgtccc gcagaccctg atggtctgcg agacagtcaa caacatcatc
     5641 gggcgcaccg tcaacccacg caacaagaac tggtcgtgcg gcggcagttc tggtggtgag
     5701 ggtgcgatcg ttgggattcg tggtggcgtc atcggtgtag gaacggatat cggtggctcg
     5761 attcgagtgc cggccgcgtt caacttcctg tacggtctaa ggccgagtca tgggcggctg
     5821 ccgtatgcaa agatggcgaa cagcatggag ggtcaggaga cggtgcacag cgttgtcggg
     5881 ccgattacgc actctgttga gggtgagtcc ttcgcctctt ccttcttttc ctgctctata
     5941 ccaggcctcc actgtcctcc tttcttgctt tttatactat atacgagacg ggcagtcact
     6001 gatgaagtaa tgttagacct ccgcctcttc accaaatccg tcctcggtca ggagccatgg
     6061 aaatacgact ccaaggtcat ccccatgccc tggcgccagt ccgagtcgga cattattgcc
     6121 tccaagatca agaacggcgg gctcaatatc ggctactaca acttcgacgg caatgtcctt
     6181 ccacaccctc ctatcctgcg cggcgtggaa accaccgtcg ccgcactcgc caaagccggt
     6241 cacaccgtga ccccgtggac gccatacaag cacgatttcg gccacgatct catctcccat
     6301 atctacgcgg ctgacggcag cgccgacgta atgcgcgata tcagtgcatc cggcgagccg
     6361 gcgattccaa atatcaaaga cctactgaac ccgaacatca aagctgttaa catgaacgag
     6421 ctctgggaca cgcatctcca gaagtggaat taccagatgg agtaccttga gaaatggcgg
     6481 gaggctgaag aaaaggccgg gaaggaactg gacgccatca tcgcgccgat tacgcctacc
     6541 gctgcggtac ggcatgacca gttccggtac tatgggtatg cctctgtgat caacctgctg
     6601 gatttcacga gcgtggttgt tccggttacc tttgcggata agaacatcga taagaagaat
     6661 gagagtttca aggcggttag tgagcttgat gccctcgtgc aggaagagta tgatccggag
     6721 gcgtaccatg gggcaccggt tgcagtgcag gttatcggac ggagactcag tgaagagagg
     6781 acgttggcga ttgcagagga agtggggaag ttgctgggaa atgtggtgac tccataggct
     6841 tctaataagt gtcagatagc aatttgcaca agaaatcaat accagcaact gtaaataagc
     6901 gctgaagtga ccatgccatg ctacgaaaga gcagaaaaaa acctgccgta gaaccgaaga
     6961 gatatgacac gcttccatct ctcaaaggaa gaatcccttc agggttgcgt ttccagtcta
     7021 gacacgtata acggcacaag tgtctctcac caaatgggtt atatctcaaa tgtgatctaa
     7081 ggatggaaag cccagaatat tggctgggtt gatggctgct tcgagtgcag tctcatgctg
     7141 ccacaggtga ctctggatgg ccccatacca ctcaacccat ggtacgagcg gtacgcttta
     7201 cgaattccca tggggagcag aaggaagaac gaaggaagga gcacagactt agattggtat
     7261 atatacgcat atgtggtgtt gaagaaacat gaaattgccc agtattctta acccaactgc
     7321 acagaacaaa aacctgcagg aaacgaagat aaatcaatgg cctcctccga ggacgtcatc
     7381 aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag
     7441 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     7501 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt ccagtacggc
     7561 tccaaggtgt acgtgaagca ccccgccgac atccccgact acaagaagct gtccttcccc
     7621 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     7681 caggactcct ccctgcagga cggctccttc atctacaagg tgaagttcat cggcgtgaac
     7741 ttcccctccg acggccccgt aatgcagaag aaaactatgg gctgggaggc ctccaccgag
     7801 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc acaaggccct gaagctgaag
     7861 gacggcggcc actacctggt ggagttcaag tccatctaca tggccaagaa gcccgtgcag
     7921 ctgcccggct actactacgt ggactccaag ctggacatca cctcccacaa cgaggactac
     7981 accatcgtgg agcagtacga gcgcgccgag ggccgccacc acctgttcct gactcacggt
     8041 atggacgaat tgtacaagtc gaagctgtaa gcttgtgctt ctaaggtatg agtcgcaaaa
     8101 ttgtttttta tttttggtct tgagtctaat atgctcgcag ctcttgcgtt gtatatggtc
     8161 gttgtcgcgt attttctgtt gtattaaaag atcaaacgag atcaagggat ggctcgcggg
     8221 ctgtctctcg cactaggagg aagaatgcct gaaaaaggaa ctttgatttt agctgtggaa
     8281 tagagatggc ttgtttgagg acgcttgtcg cttggcgcag ggacttgaat ggcagcttgt
     8341 ggaaaccgaa ggcgagaaaa gtcgacggat actgtacgtg gttctattgc cagtgcggtg
     8401 gaagcttcct tctcatatag ttcaatcctt ctttgaatct gtttgtttca tatttggact
     8461 gtttcattct ctgcttgcgc attctcatct tcgagaacga ctgcagggat tgttggttct
     8521 gtggagctga tgagcgcgcc ttgaccaccc ttgttcttgt tttgctcttt tgttctcatt
     8581 taacccgttt ctcccttcca accctttgac cttgcaacat tgtctcccag cgcgttgcca
     8641 aagcgaactt gatatcagta tagtatgacc aagtagtcta ccaaaataaa ttttagtaca
     8701 gtattgctag ttatacaaga taaattttgt acaacaatga ttatgcagag ctggtattct
     8761 gtaaggagta aattcatgca ggtacagagc gtccacgtca ggcacacgcg ctttagtgct
     8821 gggtacacat tatgctacct ttggggcaag gtgctattct aggtagggta tgcctagcaa
     8881 tgccatgatc ttatgacctc agtaatctga ccttggattt aagatcggag ttctcaaacc
     8941 ctgcgcgctc agagaattcg catgcggaga ctttagacat acctctcgtt gccgacaaat
     9001 cggcattccg tttcggtcac ggacgtaaga gttcaacaaa gccgctcacc tgtcccgttc
     9061 ggcgcttgag tttccacttc aaaagcacaa ccgctccgat atttccattg ccaccgacca
     9121 acaaaaggtt catatcgcgc aatagtcgtc caactctcac tccgcccgca ctcgatggcg
     9181 aaggatggaa gaatatccga gcggcatcgg ataaagagat ctggttcttt tcgtgaagta
     9241 acgtagggaa ccgacggaaa ctgtagagct ataggaattt agtatatcag aagtcaattg
     9301 agagaagtgt atagttagta actcacttgt gccaacaggt atttccaacc gttcttgttc
     9361 aaatttcgtc aagtcaccat ctctatgtgc aaaactggat catacaacag ctcttattgt
     9421 agctgcatct cgctctcaga gatcatccta tcttcgtcgt catccaccaa cacaggtggg
     9481 agtgctagtt gatccatgct gaggtcaagg gacacagacg gaatgggtgt agaacgcctc
     9541 aaaacaggta aggtatcagg atcggttgtc cagggggaat ccaaaccagg ttccaccttg
     9601 attcagagaa taagtatgac gtattcttgg caatgtttaa gcttgagatg agccgatgtc
     9661 aaactgggag acatgatgaa ggaggaaatg ttgctttggt gggggagaca ctataaatac
     9721 caaagagtga atggttgata ccatttgggg aaggagtgtc tatcgcagtc tctgcagacg
     9781 gcatatcgga tagcctgcag gcgcttgtgg aggatgcaca tgtgaccgag ggacacgaag
     9841 tgatccgttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
     9901 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     9961 atttgtatgt gccagccgcc tttgcgacgc tcaccgggct ggttgccctc gccgctgggc
    10021 tggcggccgt ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac
    10081 accgcggccg ccggcgttgt ggatacctcg cggaaaactt ggccctcact gacagatgag
    10141 gggcggacgt tgacacttga ggggccgact cacccggcgc ggcgttgaca gatgaggggc
    10201 aggctcgatt tcggccggcg acgtggagct ggccagcctc gcaaatcggc gaaaacgcct
    10261 gattttacgc gagtttccca cagatgatgt ggacaagcct ggggataagt gccctgcggt
    10321 attgacactt gaggggcgcg actactgaca gatgaggggc gcgatccttg acacttgagg
    10381 ggcagagtgc tgacagatga ggggcgcacc tattgacatt tgaggggctg tccacaggca
    10441 gaaaatccag catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt
    10501 aacctgcttt taaaccaata tttataaacc ttgtttttaa ccagggctgc gccctgtgcg
    10561 cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg aaccctcccg gcccgctaac
    10621 gcgggcctcc catcccccca ggggctgcgc ccctcggccg cgaacggcct caccccaaaa
    10681 atggcagcgc tggccaattc ccgagtgcgc ggaaccccta tttgtttatt tttctaaata
    10741 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    10801 aaaaggaaga gtatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa
    10861 taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga
    10921 gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat
    10981 gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc
    11041 ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc
    11101 ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg
    11161 gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta
    11221 gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc
    11281 gaaaactggg aagaggacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag
    11341 acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc
    11401 tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac
    11461 aagtggtatg acattgcctt ctgcgtccgg tcgctcaggg aggatatcgg ggaagaacag
    11521 tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat
    11581 tatattttac tggatgaatt gttttagctg tcagaccaag tttactcata tatactttag
    11641 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    11701 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    11761 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    11821 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    11881 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    11941 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    12001 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    12061 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    12121 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    12181 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    12241 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    12301 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    12361 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg cagatcctag atgtggcgca
    12421 acgatgccgg cgacaagcag gagcgcaccg acttcttccg catcaagtgt tttggctctc
    12481 aggccgaggc ccacggcaag tatttgggca aggggtcgct ggtattcgtg cagggcaaga
    12541 ttcggaatac caagtacgag aaggacggcc agacggtcta cgggaccgac ttcattgccg
    12601 ataaggtgga ttatctggac accaaggcac caggcgggtc aaatcaggaa taagggcaca
    12661 ttgccccggc gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg acgtttgacc
    12721 ggaaggcata caggcaagaa ctgatcgacg cggggttttc cgccgaggat gccgaaacca
    12781 tcgcaagccg caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc ggctcgatgg
    12841 tccagcaagc tacggccaag atcgagcgcg acagcgtgca actggctccc cctgccctgc
    12901 ccgcgccatc ggccgccgtg gagcgttcgc gtcgtcttga acaggaggcg gcaggtttgg
    12961 cgaagtcgat gaccatcgac acgcgaggaa ctatgacgac caagaagcga aaaaccgccg
    13021 gcgaggacct ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg aaacacacga
    13081 agcagcagat caaggaaatg cagctttcct tgttcgatat tgcgccgtgg ccggacacga
    13141 tgcgagcgat gccaaacgac acggcccgct ctgccctgtt caccacgcgc aacaagaaaa
    13201 tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt caacaaggac gtgaagatca
    13261 cctacaccgg cgtcgagctg cgggccgacg atgacgaact ggtgtggcag caggtgttgg
    13321 agtacgcgaa gcgcacccct atcggcgagc cgatcacctt cacgttctac gagctttgcc
    13381 aggacctggg ctggtcgatc aatggccggt attacacgaa ggccgaggaa tgcctgtcgc
    13441 gcctacaggc gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg gaatcggtgt
    13501 cgctgctgca ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt tgccaggtcc
    13561 tgatcgacga ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa ttcatatggg
    13621 agaagtaccg caagctgtcg ccgacggccc gacggatgtt cgactatttc agctcgcacc
    13681 gggagccgta cccgctcaag ctggaaacct tccgcctcat gtgcggatcg gattccaccc
    13741 gcgtgaagaa gtggcgcgag caggtcggcg aagcctgcga agagttgcga ggcagcggcc
    13801 tggtggaaca cgcctgggtc aatgatgacc tggtgcattg caaacgctag ggccttgtgg
    13861 ggtcagttcc ggctgggggt tcagcagccc ctgctcggat ctgttggacc ggacagtagt
    13921 catggttgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag
    13981 ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact
    14041 taataacaca ttgcggacgt ttttaatgta ctggggttga acactct
//
