LOCUS       sequence-306061-       14169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, 
            activating DsRed-SKL with promoter 1xQUAS+CP_pcbC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14169)
  AUTHORS   Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
  TITLE     Synthetic control devices for gene regulation in Penicillium 
            chrysogenum.
  JOURNAL   Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 
            10.1186/s12934-019-1253-3.
  PUBMED    31739777
REFERENCE   2  (bases 1 to 14169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2019-11-18"; volume: "18(1):203. doi"; pages: " 
            10.1186/s12934-019-1253-3"
COMMENT     Sequence Label: pVE2_1_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_1xQUAS
FEATURES             Location/Qualifiers
     source          1..14169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2781..2891
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2949..2970)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3210..3229)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3557..3578
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3621..3641
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4813..4928
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             7439..8113
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(7569..7587)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7619..7639)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8031..8054
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    9947..9971
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      10059..10769
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10790..10894
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10895..11689
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11860..12448
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12349..12368
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12784..13932
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14078..14102
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
       61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
      121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
      181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
      241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
      301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
      361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
      421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
      481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
      541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
      601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
      661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
      721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
      781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
      841 gaattcaagc ttggaggagt tatagacggt ccggcatagg taaggagaga ttagatattt
      901 cgacttcccg attccttagt ctccttagtc tggaaactga gaaaggcctg aactcgttca
      961 atctattagc tctaatcacg tagtctcaac tagaatcctg aacaggcgcc ggaaccagtc
     1021 caaatgcgtg gagtacagca agtccagtca aatcacgatt agaatggcca tatataagca
     1081 aaaaaccctt ggacaatacg tccgatcgac accaaagcaa tctggtacat cacccttctt
     1141 atatacaggg tagccggaat tgtattgact ctatacgaag ccgcaggttt accagcacag
     1201 aggtgcgata gctacacgaa tgtagagtga aggcattaca accgattgct aagtgtcata
     1261 gaggctaagc ttcacggctc aaggagtctg tggaggatac ctgatataag aattgagagt
     1321 tatactccgg aggttatgtt agtacctcaa aaatgtgaag tacaacgaaa ctcgaacttc
     1381 gaatgacaat agcttggctg gctgttagtc aactgaggta caacgtcatc ttgcaattag
     1441 ttatgttctt tgctagatag tgagctgctg taaacttgta gttcttagaa acatgtctga
     1501 agtctagagt cgagacttgt gattggtgta aattagtggg ggtccggagc ggaataacaa
     1561 tcgaggtcag cctcctgggt ctgtcagtaa gctaaccacg aaaggacaag cgtagacgag
     1621 gtcacggggt actggcctag ccggcttcgg ctagcgccca cgaaaatagg gcaacgtcga
     1681 agcgagagac acacacggat tccacgatcg ccgacgacaa gcactcaact ccacgacaac
     1741 ccacgacaac ccttgccgct gcgttcttgg tgaagggctg caggattaga ggtgagtatt
     1801 ttattttctg gctattgtga tctttttccc ccccggtttc cgctggttct atcttgtcga
     1861 gcgagtcggt gcgccttcga tcggtctgtc aaatttccat ccgtccactc gttcacccgg
     1921 tatcgataca agagggaatg gagcattacg acaggggagc agaggcagaa ctctcgatgt
     1981 tgctactttt tttgcttgga acaacttcct tgaaagattc ttttctgaat gcggctggga
     2041 aactgcgaat tggatatcca gcgaaaggtt accgacagac tcacggccat cgataccact
     2101 cagcgggata gaattgaagg cattcggaca ttagatgatc agcttcgctt gtttcgactc
     2161 gtgctaatca attatccctg gacagtcccc cacatagaca gcaaacaatg ccacccaagc
     2221 gcaaaacgct taacgctgcg gctgaggcta acgctcatgc cgacggacac gccgacggaa
     2281 acgccgacgg acacgtggcc aatacggccg cgtcctcgaa taatgcgagg ttcgctgatc
     2341 tcactaacat cgatactccg ggtctgggac ccacaactac gaccctgctc gtggaaccag
     2401 cacgctcaaa gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca gcccgagaga
     2461 aatgcgacgg aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg tcctgcactt
     2521 atcaggcttc gccgaaaaag aggggagttc aaaccggtta tattcgtacg ctggagctcg
     2581 ccctcgcctg gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat aacctcctcg
     2641 tccgtgacgc cggacaagga tcagctctgc tcgttggtaa agattcgccg gctgccgagc
     2701 gactccatgc ccgttgggct actagccgtg tcaataagag cattacccgc ctcctcaggt
     2761 ttgaaagcgg cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg
     2821 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     2881 ttgacatgct ccccgggttc gttatggtga gcaagggcga ggagctgttc accggggtgg
     2941 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     3001 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     3061 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     3121 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3181 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3241 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3301 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3361 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3421 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3481 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3541 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3601 gcatggacga gctgtacaag ccaaagaaga agcggaaggt ctaagcttac ttctttatcg
     3661 gttctctctt acgacttttt gaatggaacg tttccttctt ctcaggcggg cctatctttg
     3721 ggccgaagct cttttccttg tactgtagga cctggttgat aatgattccc aaaaagacat
     3781 ccagcatgtc agttacttgc attcgtcagt ctatacaaaa gcaatggttt agagaaattt
     3841 tgaactttat acatggtttt atttgttgct tcacggccgt accttctgga aatccacggt
     3901 aggagtgtca atttgcgttt ttgataatcc ttccaaggtt cttctcgaag tagttgttct
     3961 ataattgctt cacagctacc atggaacatc ccaagcaccg ctactagaat tcgagctcgg
     4021 agtggatccc ccgggctgca ggaattcgag ctctgtacag tgaccggtga ctctttctgg
     4081 catgcggaga gacggacgga cgcagagaga agggctgagt aataagcgcc actgcgccag
     4141 acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc
     4201 cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg
     4261 agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt
     4321 gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg
     4381 atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc
     4441 gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc
     4501 ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac
     4561 cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc
     4621 tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca
     4681 aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct
     4741 cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt
     4801 tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc
     4861 tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc
     4921 ttgagcagac atcaccatgc ctcaatcctg ggaagaactg gccgctgata agcgcgcccg
     4981 cctcgcaaaa accatccctg atgaatggaa agtccagacg ctgcctgcgg atgacagcgt
     5041 tattgatttc ccaaagaaat cggggatcct ttcagaggcc gaactgaaga tcacagaggc
     5101 ctccgctgca gatcttgtgt ccaagctggc ggccggagag ttgacctcgg tggaagttac
     5161 gctagcattc tgtaaacggg cagcaatcgc ccagcagtta gtagggtccc ctctacctct
     5221 cagggagatg taacaacgcc accttatggg actatcaagc tgacgctggc ttctgtgcag
     5281 acaaactgcg cccacgagtt cttccctgac gccgctctcg cgcaggcaag ggaactcgat
     5341 gaatactacg caaagcacaa gacacccgtt ggtccactcc atggcctccc catctctctc
     5401 aaagaccagc ttcgagtcaa ggtacaccgt tgcccctaag tcgttagatg tccctttttg
     5461 tcagctaaca tatgccacca gggctacgaa acatcaatgg gctacatctc atggctaaac
     5521 aagtacgacg aaggggactc ggttctgaca accatgctcc gcaaagccgg tgccgttttc
     5581 tacgtcaaga cctctgtccc gcagaccctg atggtctgcg agacagtcaa caacatcatc
     5641 gggcgcaccg tcaacccacg caacaagaac tggtcgtgcg gcggcagttc tggtggtgag
     5701 ggtgcgatcg ttgggattcg tggtggcgtc atcggtgtag gaacggatat cggtggctcg
     5761 attcgagtgc cggccgcgtt caacttcctg tacggtctaa ggccgagtca tgggcggctg
     5821 ccgtatgcaa agatggcgaa cagcatggag ggtcaggaga cggtgcacag cgttgtcggg
     5881 ccgattacgc actctgttga gggtgagtcc ttcgcctctt ccttcttttc ctgctctata
     5941 ccaggcctcc actgtcctcc tttcttgctt tttatactat atacgagacg ggcagtcact
     6001 gatgaagtaa tgttagacct ccgcctcttc accaaatccg tcctcggtca ggagccatgg
     6061 aaatacgact ccaaggtcat ccccatgccc tggcgccagt ccgagtcgga cattattgcc
     6121 tccaagatca agaacggcgg gctcaatatc ggctactaca acttcgacgg caatgtcctt
     6181 ccacaccctc ctatcctgcg cggcgtggaa accaccgtcg ccgcactcgc caaagccggt
     6241 cataccgtga ccccgtggac gccatacaag cacgatttcg gccacgatct catctcccat
     6301 atctacgcgg ctgacggcag cgccgacgta atgcgcgata tcagtgcatc cggcgagccg
     6361 gcgattccaa atatcaaaga cctactgaac ccgaacatca aagctgttaa catgaacgag
     6421 ctctgggaca cgcatctcca gaagtggaat taccagatgg agtaccttga gaaatggcgg
     6481 gaggctgaag aaaaggccgg gaaggaactg gacgccatca tcgcgccgat tacgcctacc
     6541 gctgcggtac ggcatgacca gttccggtac tatgggtatg cctctgtgat caacctgctg
     6601 gatttcacga gcgtggttgt tccggttacc tttgcggata agaacatcga taagaagaat
     6661 gagagtttca aggcggttag tgagcttgat gccctcgtgc aggaagagta tgatccggag
     6721 gcgtaccatg gggcaccggt tgcagtgcag gttatcggac ggagactcag tgaagagagg
     6781 acgttggcga ttgcagagga agtggggaag ttgctgggaa atgtggtgac tccataggct
     6841 tctaataagt gtcagatagc aatttgcaca agaaatcaat accagcaact gtaaataagc
     6901 gctgaagtga ccatgccatg ctacgaaaga gcagaaaaaa acctgccgta gaaccgaaga
     6961 gatatgacac gcttccatct ctcaaaggaa gaatcccttc agggttgcgt ttccagtcta
     7021 gacacgtata acggcacaag tgtctctcac caaatgggtt atatctcaaa tgtgatctaa
     7081 ggatggaaag cccagaatat tggctgggtt gatggctgct tcgagtgcag tctcatgctg
     7141 ccacaggtga ctctggatgg ccccatacca ctcaacccat ggtacgagcg gtacgcttta
     7201 cgaattccca tggggagggg taatcgctta tccccatcgt ataatgtctc caggttgtct
     7261 cagcataaac accccgcccc cgctcaggca cacaggaaga gagctcaggt cgtttccatt
     7321 gcgtccatac tcttcactca ttgtcatctg caggagaact tcccctgtcc ctttgccaag
     7381 ccctctcttc gtcgttgtcc acgccttcaa gttttcacca ttatttttct agacaccaat
     7441 ggcctcctcc gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc
     7501 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     7561 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
     7621 gtccccccag ttccagtacg gctccaaggt gtacgtgaag caccccgccg acatccccga
     7681 ctacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     7741 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggctcct tcatctacaa
     7801 ggtgaagttc atcggcgtga acttcccctc cgacggcccc gtaatgcaga agaaaactat
     7861 gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat
     7921 ccacaaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca agtccatcta
     7981 catggccaag aagcccgtgc agctgcccgg ctactactac gtggactcca agctggacat
     8041 cacctcccac aacgaggact acaccatcgt ggagcagtac gagcgcgccg agggccgcca
     8101 ccacctgttc ctgactcacg gtatggacga attgtacaag tcgaagctgt aagcttgtgc
     8161 ttctaaggta tgagtcgcaa aattgttttt tatttttggt cttgagtcta atatgctcgc
     8221 agctcttgcg ttgtatatgg tcgttgtcgc gtattttctg ttgtattaaa agatcaaacg
     8281 agatcaaggg atggctcgcg ggctgtctct cgcactagga ggaagaatgc ctgaaaaagg
     8341 aactttgatt ttagctgtgg aatagagatg gcttgtttga ggacgcttgt cgcttggcgc
     8401 agggacttga atggcagctt gtggaaaccg aaggcgagaa aagtcgacgg atactgtacg
     8461 tggttctatt gccagtgcgg tggaagcttc cttctcatat agttcaatcc ttctttgaat
     8521 ctgtttgttt catatttgga ctgtttcatt ctctgcttgc gcattctcat cttcgagaac
     8581 gactgcaggg attgttggtt ctgtggagct gatgagcgcg ccttgaccac ccttgttctt
     8641 gttttgctct tttgttctca tttaacccgt ttctcccttc caaccctttg accttgcaac
     8701 attgtctccc agcgcgttgc caaagcgaac ttgatatcag tatagtatga ccaagtagtc
     8761 taccaaaata aattttagta cagtattgct agttatacaa gataaatttt gtacaacaat
     8821 gattatgcag agctggtatt ctgtaaggag taaattcatg caggtacaga gcgtccacgt
     8881 caggcacacg cgctttagtg ctgggtacac attatgctac ctttggggca aggtgctatt
     8941 ctaggtaggg tatgcctagc aatgccatga tcttatgacc tcagtaatct gaccttggat
     9001 ttaagatcgg agttctcaaa ccctgcgcgc tcagagaatt cgcatgcgga gactttagac
     9061 atacctctcg ttgccgacaa atcggcattc cgtttcggtc acggacgtaa gagttcaaca
     9121 aagccgctca cctgtcccgt tcggcgcttg agtttccact tcaaaagcac aaccgctccg
     9181 atatttccat tgccaccgac caacaaaagg ttcatatcgc gcaatagtcg tccaactctc
     9241 actccgcccg cactcgatgg cgaaggatgg aagaatatcc gagcggcatc ggataaagag
     9301 atctggttct tttcgtgaag taacgtaggg aaccgacgga aactgtagag ctataggaat
     9361 ttagtatatc agaagtcaat tgagagaagt gtatagttag taactcactt gtgccaacag
     9421 gtatttccaa ccgttcttgt tcaaatttcg tcaagtcacc atctctatgt gcaaaactgg
     9481 atcatacaac agctcttatt gtagctgcat ctcgctctca gagatcatcc tatcttcgtc
     9541 gtcatccacc aacacaggtg ggagtgctag ttgatccatg ctgaggtcaa gggacacaga
     9601 cggaatgggt gtagaacgcc tcaaaacagg taaggtatca ggatcggttg tccaggggga
     9661 atccaaacca ggttccacct tgattcagag aataagtatg acgtattctt ggcaatgttt
     9721 aagcttgaga tgagccgatg tcaaactggg agacatgatg aaggaggaaa tgttgctttg
     9781 gtgggggaga cactataaat accaaagagt gaatggttga taccatttgg ggaaggagtg
     9841 tctatcgcag tctctgcaga cggcatatcg gatagcctgc aggcgcttgt ggaggatgca
     9901 catgtgaccg agggacacga agtgatccgt ttaaactatc agtgtttgac aggatatatt
     9961 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt
    10021 gaaaaggttt atccgttcgt ccatttgtat gtgccagccg cctttgcgac gctcaccggg
    10081 ctggttgccc tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg
    10141 ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt gtggatacct cgcggaaaac
    10201 ttggccctca ctgacagatg aggggcggac gttgacactt gaggggccga ctcacccggc
    10261 gcggcgttga cagatgaggg gcaggctcga tttcggccgg cgacgtggag ctggccagcc
    10321 tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc cacagatgat gtggacaagc
    10381 ctggggataa gtgccctgcg gtattgacac ttgaggggcg cgactactga cagatgaggg
    10441 gcgcgatcct tgacacttga ggggcagagt gctgacagat gaggggcgca cctattgaca
    10501 tttgaggggc tgtccacagg cagaaaatcc agcatttgca agggtttccg cccgtttttc
    10561 ggccaccgct aacctgtctt ttaacctgct tttaaaccaa tatttataaa ccttgttttt
    10621 aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct
    10681 cgaaccctcc cggcccgcta acgcgggcct cccatccccc caggggctgc gcccctcggc
    10741 cgcgaacggc ctcaccccaa aaatggcagc gctggccaat tcccgagtgc gcggaacccc
    10801 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    10861 ataaatgctt caataatatt gaaaaaggaa gagtatggct aaaatgagaa tatcaccgga
    10921 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
    10981 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
    11041 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
    11101 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
    11161 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
    11221 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
    11281 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
    11341 cgatctggcc gatgtggatt gcgaaaactg ggaagaggac actccattta aagatccgcg
    11401 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
    11461 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
    11521 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgctcag
    11581 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
    11641 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagc tgtcagacca
    11701 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    11761 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    11821 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    11881 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    11941 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    12001 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    12061 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    12121 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    12181 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    12241 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    12301 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    12361 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    12421 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    12481 ggcagatcct agatgtggcg caacgatgcc ggcgacaagc aggagcgcac cgacttcttc
    12541 cgcatcaagt gttttggctc tcaggccgag gcccacggca agtatttggg caaggggtcg
    12601 ctggtattcg tgcagggcaa gattcggaat accaagtacg agaaggacgg ccagacggtc
    12661 tacgggaccg acttcattgc cgataaggtg gattatctgg acaccaaggc accaggcggg
    12721 tcaaatcagg aataagggca cattgccccg gcgtgagtcg gggcaatccc gcaaggaggg
    12781 tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga cgcggggttt
    12841 tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc
    12901 ttccagtccg tcggctcgat ggtccagcaa gctacggcca agatcgagcg cgacagcgtg
    12961 caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctt
    13021 gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg aactatgacg
    13081 accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga ggccaagcag
    13141 gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc cttgttcgat
    13201 attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg ctctgccctg
    13261 ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt cattttccac
    13321 gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa
    13381 ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc
    13441 ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg gtattacacg
    13501 aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac gtccgaccgc
    13561 gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag
    13621 aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac
    13681 cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc ccgacggatg
    13741 ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac cttccgcctc
    13801 atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc
    13861 gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga cctggtgcat
    13921 tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagcagc ccctgctcgg
    13981 atctgttgga ccggacagta gtcatggttg atgggctgcc tgtatcgagt ggtgattttg
    14041 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
    14101 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactggggtt
    14161 gaacactct
//
