LOCUS       sequence-306068-       13452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG 
            assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13452)
  AUTHORS   Nonet ML
  TITLE     Efficient Transgenesis in Caenorhabditis elegans Using Flp 
            Recombinase-Mediated Cassette Exchange.
  JOURNAL   Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 
            10.1534/genetics.120.303388.
  PUBMED    32513816
REFERENCE   2  (bases 1 to 13452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2020 Jun 8. pii: genetics.120.303388. doi: 
            10.1534/genetics.120.303388."
COMMENT     Sequence Label: pNM3746
FEATURES             Location/Qualifiers
     source          1..13452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      36..623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     524..543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(635..662)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(686..1501)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1409..1428
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1502..1604)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(1605..1636)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     protein_bind    complement(1719..1752)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1762..1791
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3608..3931)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5849..5873
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5876..6901
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5876..6901
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6902..6926)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7279..7312)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    11532..11565
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(11612..11640)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     12388..12408
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(13201..13234)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(13431..13448)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13431..13447)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgggcctttc ttcggtagaa aatcaaagga tcttcttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaaacgcca gcaacgcaga aaggcccacc cgaaggtgag
      661 ccaggtgatt acatttaggt cctcattaga aaaactcatc gagcatcaag tgaaactgca
      721 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
      781 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
      841 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
      901 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaagagt ttatgcattt
      961 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcaccaa
     1021 ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgccgttaa
     1081 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     1141 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttccctggga
     1201 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     1261 gaggcataaa ttccgtcagc cagtttagcc tgaccatctc atctgtaaca tcattggcaa
     1321 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat
     1381 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     1441 catccatgtt ggaatttaat cgcggcttca agcaagacgt ttcccgttga atatggctca
     1501 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     1561 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaagtcaa aagcctccgg
     1621 tcggaggctt ttgactttct gctatggagg tcaggtatga tttttgaatt cgctcttcgg
     1681 cgacgtacga actaggctgt ttcgaaggta agtttaaaat aacttcgtat agcatacatt
     1741 atacgaagtt attttcaggg agagcaaaag cttatctccg aggaggacct ctaaggatga
     1801 tcgacgccaa cgtcgttgaa ttttcaaatt ttaaatactg aatatttgtt ttttttccta
     1861 ttatttattt attctctttg tgtttttttt cttgctttct aaaaaattaa ttcaatccaa
     1921 atctaaacat ttttttttct ctttccgtct cccaattcgt attccgctcc tctcatctga
     1981 acacaatgtg caagtttatt tatcttctcg ctttcatttc attaggacgt ggggggaatt
     2041 ggtggaaggg ggaaacacac aaaaggatga tggaaatgaa ataaggacac acaatatgca
     2101 acaacattca attcagaaat atggaggaag gtttaaaaga aaacataaaa atatatagag
     2161 gaggaaggaa actagctgcc ctcgattgtc tatcttccat tctcaccaag tttcaacaac
     2221 tttattcagt cattcttctc tggttcattt cccaaccaat cttcccgaat aaagcccatc
     2281 tgtcctttcg gccacattcc tcacctacat ccgtactcct tatctcccgt tcttatccag
     2341 tcttttatcg tctctgcgtc tcgcacactg tctcgtcggt aatgtaaaaa aaaaacaaaa
     2401 aaaacttgaa aacttgcgga aagatttcct aataaaagga gacgaatccg gtaggaaatc
     2461 gacaagtctc tgcttctcga agactaggtg agttacattt ctttggccga attattttga
     2521 aatcctaaat ttgataattt cagagatgtc tgtaaaactt gcgtgttatg taacggcttc
     2581 ggtcactgtc gccactctta tggtatgctt catgaccatg tcaaccatct actcggaggt
     2641 tgatggattc agagagaagc ttgataccga gatgaatgtg ttcagagtga gttaaattat
     2701 attttgaatt ttaaataatt tttaattttc tagcaatcta ccaatggatt gtggaaggac
     2761 atagttgtca tcggaagatc tagcaagcgt gtccgttgtc aatatgaaga gaccaacgct
     2821 accccaactc cacatgctga tggatcccca tctgctccac caggtcaacc accagcagtt
     2881 ccaccagtct tcaaccagcc aaagactcca aatggagcca atggaaatgg accaacctgc
     2941 aactgcaatg ctgataacaa gtgcccagct ggaccatccg gaccaaaggg agttccagga
     3001 gttccaggac tcgacggagt tccaggactt gacggtgttc caggagttgg agctgatgat
     3061 atcgctccac aacgcgagtc tgtcggatgc ttcacttgcc cacaaggacc agttggacca
     3121 ccaggagctc ttggaagacc aggaccacgt ggacttccag gaccaagagg acaaaatgga
     3181 aacccaggaa gagatggaca accaggacat ccaggagagc aaggatcatc cggtcaaatc
     3241 ggaaagatcg gagagccagg accaccagga gagaagggac gcgacgccga gcatccaatc
     3301 ggaagaccag gaccaaaggg accaagagga gatcaaggac caacaggacc agctggacag
     3361 aacggtcttc acggaccacc aggagagcca ggaaccgttg gaccagaagg accatctgga
     3421 aagcaaggac gtcaaggacc agacggaacc cagggagaga ctggaccaga cggaagacca
     3481 ggaaaggatg ccgagtactg ccagtgccca gacaagtctc caccatcaga ggctgtcaac
     3541 gccaaccgtg gatacagaaa tatctaaatt gttggtgttt tctaataaaa tatttgagat
     3601 tcagtgagac ttttttcttg gcggcacaat aaagtttcct ctttttgttg ggtcactcaa
     3661 cgaacacaat acaaatattt gagcgctcgc atttttctca acctttcggg tttaaagcac
     3721 ttcaaaatgg aattgaagag agattgtgaa agtacatcag acagaatcac attttgatag
     3781 cattcacttc actcagatgc aaacttttca gtgactataa aaagtcataa aataataaca
     3841 ttaaaaaagc aaataaatta gcgagagaat tttttgacaa aaagaaagaa gagtgataga
     3901 gaagaaggga atgcttgaaa ggattttgca tttatcttaa tcaccgtctt cgagaagacg
     3961 aaccatggcg ccagtttcag aatccaagtt tctaatatag ttcatcacaa tgttgacgtt
     4021 ggtccaacct ccggcttgca taatctctgg gatggaaact ccagcgcgag ccatgtcacg
     4081 ggcagctccg acacgggcgg aatgtccaga ccaagccaag taacgttgtc cggaatcatc
     4141 cttggctccg tagatgagac ggtgggtagc ttcgaagatt cctgaaaatt tattttcagc
     4201 ttattcttag ctaatatgtt gtttaaactt accttcaaga gctcgagtcg agagttgtga
     4261 tgttgcactc ggggcagcaa ctccgttctt gcgaacacga cagaacaggt agttgtttgg
     4321 atcatcggcg actccggaaa ccgaaatcca gcgctcaacg agcttggtaa ctccaaggga
     4381 gagcgctttt tcgactccgg cagtcgagac cagcgttttt gtacgtccga tgtggatgag
     4441 cattcttcct ccgtctgtgc gactaatatc cttgacgcga atgcgggcaa tttcggcgat
     4501 acggagaagt gtgttgtaag caattccgag gaaggcgagg tttcggatgt cctggcaacg
     4561 atcggaattt tccataagag aacggacctg atcgaagtcg gttctgaaaa tttcgtcaag
     4621 tcttaggatt gaatacatta atatttaaac ttacctttcg aaggcgaggg cttgcttggc
     4681 tctctctcca gcatcaacat tttccttgcg gatacgtctc attacgagag atacggcatt
     4741 agaatctgat gggcgtggaa gtccagatcg gcgatggagc atattgagct gtccgagatg
     4801 ttgttggatc gtcttcaccg ccaatccacg agcttggagg tagagcaagt agtcacgaac
     4861 gtcttctggc tcggctggga accacttgcg gttgttaagc ttgcaccagg cagcccaaga
     4921 acggcacact gagagaagca tcttccaggt gtgctcggag aaggcttgtc tgtcacggaa
     4981 catatccatg aggttcttgc gaacttcatc acttgtcgcg tcaactggaa gtgctggaag
     5041 attttgatga acagtgagaa gatttgacat attttcgaag ttttttagat gcactagaac
     5101 aaagcgtgtt ggcttcctct gagcccgctt tccttatata cccgcattct gcagccttac
     5161 agaatgttct agaaggtcct agatgcattc gtttgaaaat actcccggtg ggtgcaaaga
     5221 gacgcagacg gaaaatgtat ctgggtctct ttattgtgta cactactttt ccatgtaccg
     5281 aatgtgagtc gccctccttt tgcaacaagc agctcgaatg ttctagaaaa aggtggaaaa
     5341 tagtataaat accgttgaaa ataaataccg aacaacattt gctctaattg tgaaattaga
     5401 aatcttcaaa ctataatcat gtcactttac cactatttcc gtccagctca acgttccgtt
     5461 tttggtgatc atttttgctt tcgtcgtaaa tctacacacg cgtctcttcc gtgcgagagt
     5521 ccaagccagc agccaaattc gttgactgag tattcaacgt ttatacgttg tcggcaacga
     5581 gaaataggaa aatgcatcgg gaaatgttct tttttcgatt ttttccaagg ttttgacaaa
     5641 ttttaccacg aattttgcta tgttttcaat taaaaaatat gttattcaac tgtttctatg
     5701 aggaaaataa ggctttgcat gtaattttct tattcagcat aatttttaat taatttgaat
     5761 tttctgtcct aacgtttatt ttgttttctt ggttatgact gatctgaaat taatttttga
     5821 attttaaggt aatatgtcag gcggtgccgc aagtttgtac aaaaaagcag gctccatgaa
     5881 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     5941 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     6001 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     6061 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     6121 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     6181 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     6241 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     6301 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     6361 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     6421 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     6481 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     6541 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     6601 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     6661 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     6721 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     6781 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     6841 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
     6901 gacccagctt tcttgtacaa agtgggtcca attactcttc aacatcccta catgctcttt
     6961 ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg
     7021 ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat
     7081 taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta
     7141 tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt
     7201 ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt
     7261 ttatgaccgc aatttttaat aacttcgtat agcatacatt atacgaagtt attttcaggg
     7321 tgggagaaaa tgcggagcat gcggccgcat atcagttttt aaaaaattaa accataaaac
     7381 aaataatata acccaatttt tacatcaaac cacaagaaaa aaatacattt gggcccacgg
     7441 ataaagaaat taaaaaaata cattttttaa aggcgcaccg aattaaaatt catttgggtc
     7501 ttaccgcgta taccgtactc cgtttgtttg atcatttttg tcagcgctgg cggttgtttt
     7561 ttcatttcat ttctgcttca aagacgtttt ctcgaataat ttttcgttta ttctcttttt
     7621 taaaattaat ttctagccgt aaatgttata aattcaccca tttaacgcaa atttcatggt
     7681 aatctcatgg aaaaatgcag tttctttgtt aaagaaagct taaatagcaa aaattccccg
     7741 actttcccca aaatcctgct cgattttccg ttttctcatt gtattctctc ttaattaatt
     7801 ttatcgataa tcaattgaat gtttcagaca gagaggtacc aaaaatgcca caattcgaca
     7861 ttctctgtaa gacaccacca aaagtcctcg tccgtcaatt cgtcgagcgt ttcgagcgtc
     7921 catccggaga gaagatcgcc ctctgcgccg ccgagctcac ctacctctgc tggatgatca
     7981 cccacaacgg aaccgccatc aagcgtgcca ccttcatgtc ctacaacacc atcatctcca
     8041 actccctctc cttcgacatc gtcaacaagt ccctccaatt caagtacaag acccaaaagg
     8101 ccaccatcct cgaggcctcc ctcaaggtaa gtttaaacat atatatacta actaaccctg
     8161 attatttaaa ttttcagaag ctcatcccag cctgggagtt caccatcatc ccatactacg
     8221 gacaaaagca ccaatccgac atcaccgaca tcgtctcctc cctccaactc caattcgagt
     8281 cctccgagga ggccgacaag ggaaactccc actccaagaa gatgctcaag gccctcctct
     8341 ccgagggaga gtccatctgg gagatcaccg agaagatcct caactccttc gagtacacct
     8401 cccgtttcac caagaccaag accctctacc aattcctctt cctcgccacc ttcatcaact
     8461 gcggacgttt ctccgacatc aagaacgtcg acccaaagtc cttcaagctc gtccaaaaca
     8521 agtacctcgg agtcatcatc caatgcctcg tcaccgagac caagacctcc gtctcccgtc
     8581 acatctactt cttctccgcc cgtggacgta tcgacccact cgtctacctc gacgagttcc
     8641 tccgtaactc cgagccagtc ctcaagcgtg tcaaccgtac cggaaactcc tcctccaaca
     8701 agcaagagta ccaactcctc aaggacaacc tcgtccgttc ctacaacaag gccctcaaga
     8761 agaacgcccc atactccatc ttcgccatca agaacggacc aaagtcccac atcggacgtc
     8821 acctcatgac ctccttcctc tccatgaagg taagtttaaa catgatttta ctaactaact
     8881 aatctgattt aaattttcag ggactcaccg agctcaccaa cgtcgtcgga aactggtccg
     8941 acaagcgtgc ctccgccgtc gcccgtacca cctacaccca ccaaatcacc gccatcccag
     9001 accactactt cgccctcgtc tcccgttact acgcctacga cccaatctcc aaggagatga
     9061 tcgccctcaa ggacgagacc aacccaatcg aggagtggca acacatcgag caactcaagg
     9121 gatccgccga gggatccatc cgttacccag cctggaacgg aatcatctcc caagaggtcc
     9181 tcgactacct ctcctcctac atcaaccgtc gtatctaacg cgtgctgtct catcctactt
     9241 tcacctagtt aactgcttgt cttaaaatct atgcttctct ttagtatcta aaattttcct
     9301 agaagcttac aagtatataa atggtctctt ctcaataaag gttgtatatt tattcatctt
     9361 attgaatctg ccatttcctc gtttttgcga gtttatatac cttccaattt tctttctatt
     9421 gtattttcaa cttctaattt taattcaggg aaactgcttc aacgcatcat ggtgtcgaag
     9481 ggagaagagg ataacatggc ttcactccca gctacacacg aactccacat cttcggatcg
     9541 atcaacggag tggatttcga tatggtcgga caaggtaagt ttaaacatat atatactaac
     9601 taaccctgat tatttaaatt ttcaggaact ggaaacccaa acgatggata cgaggaactc
     9661 aacctcaagt cgacaaaggg agatctgcaa ttctcgccat ggattctcgt gccacacatc
     9721 ggatacggat tccaccaata cctcccatac ccaggtaagt ttaaactgag ttctactaac
     9781 taacgagtaa tatttaaatt ttcagatgga atgtcaccat tccaagctgc catggtggat
     9841 ggatcgggat accaagttca ccgaacaatg caattcgagg atggagcctc gctcacagtg
     9901 aactaccgat acacatacga gggatcgcac atcaaggtaa gtttaaacag ttcggtacta
     9961 actaaccata catatttaaa ttttcaggga gaggctcaag ttaagggaac aggattccca
    10021 gctgatggac cagtgatgac aaactcactc acagctgctg attggtgccg atcgaaaaag
    10081 acatacccaa atgataagac aatcatctcg acattcaagt ggtcgtacac tactggaaac
    10141 ggaaagcgat accgatcgac agcccgaaca acatacacat tcgctaagcc aatggccgcc
    10201 aactacctca aggtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt
    10261 cagaatcaac caatgtacgt gttccgaaag acagaactca agcactcaaa gacagagctg
    10321 aacttcaaag agtggcaaaa ggccttcaca gatgtgatgg gaatggatga actctacaag
    10381 taacgcgtat gtatctgcat atccttcaaa tgttgtccat attgtatcag taaattataa
    10441 tgcccccttt ttatatattc tccttatcat gtaaatgttc tattgatttt ttgctggtgt
    10501 aaaacgtttt tatagtaaat cctctccaca gtgaatttgt taaattgtat caaaacaaga
    10561 cattgattaa atttgttcga gacttgtgga ttgtaaaata ttttgcagaa aatggcaata
    10621 ttcatttaga aacattactt ccataatagg aaaaactatt tatgagtttt catgtgccta
    10681 tgcaagttcc cactctgtgt gaatttcatc atacaaattg agcaaacata aggtttatgt
    10741 ccagtatggg ttctcagatg ttttgtaaga gttgagctgt cggtgaatgc cttctcacat
    10801 tgagcacatg gatacggtct ctctccagtg tgtgttctga aataatgaaa aatagtcttg
    10861 caatatgttt ctactattcg agaaaaaatg taattttttt tcttatggta acccggcttt
    10921 ccccagcgga gagaagaacc cagcatctta ataaaattat ataattttga ataaaatcaa
    10981 attaaaaata agattaattg aaacacaaca atagaatgca gaaaattact cataagaaat
    11041 aatttatttt gaaagaagtt gtgtcgattt acgataaaaa ctacacgcaa acgaggctcc
    11101 gcgtttacac cgtttggcaa tggagcgcgc ttgctattag attttaggcc agctcaacga
    11161 aagtttaatt tatttttctt ctttttttcc atttttttga gtaaaatatc gaattttttg
    11221 tattaattct gtaaatttta taatttgcaa ctattttggc gtattctgct tgtttttatc
    11281 agcgaatttt tatgcatttc agtcaaaagt ttgagcagga aaaatgacat ttaggacttt
    11341 gtaaaattgt attcttaaat ttgcttgaaa acctacgtgt acaattgatt ttattgaatt
    11401 tttcttacgt ttcccgttgt atcttattgt atttaacttt catcataaaa taatttattt
    11461 ccaggtaaaa tatccgacgc tctcgtggat ccccgggatt ggctcagaaa aaaaggtacg
    11521 agcaggctta cgaagttcct attctctaga aagtatagga acttcggtac cggtagaaaa
    11581 aatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
    11641 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
    11701 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat gggtaagttt
    11761 aaacatatat atactaacta accctgatta tttaaatttt cagccaacac ttgtcactac
    11821 tttctgttat ggtgttcaat gcttctcgag atacccagat catatgaaac ggcatgactt
    11881 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
    11941 cgggaactac aagacacgta agtttaaaca gttcggtact aactaaccat acatatttaa
    12001 attttcaggt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
    12061 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
    12121 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttgtaagttt
    12181 aaacatgatt ttactaacta actaatctga tttaaatttt cagaacttca aaattagaca
    12241 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
    12301 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
    12361 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
    12421 tacacatggc atggatgaac tatacaaatg cccgggggat cggtggagct ccaccggtgg
    12481 cggccgctct agaactagtc caccaaagcc atctgccaag ggagccaaga aggccgccaa
    12541 gaccgtcgtt gccaagccaa aggacggaaa gaagagacgt catgcccgca aggaatcgta
    12601 ctccgtctac atctaccgtg ttctcaagca agttcaccca gacaccggag tctcctccaa
    12661 ggccatgtct atcatgaact ccttcgtcaa cgatgtattc gaacgcatcg cttcggaagc
    12721 ttcccgtctt gctcattaca acaaacgctc aacgatctca tcccgcgaaa ttcaaaccgc
    12781 tgtccgtttg attctcccag gagaacttgc caagcacgcc gtgtctgagg gaaccaaggc
    12841 cgtcaccaag tacacttcca gcaagtaaac tagtggatct gcaacgcgtg gagctagcag
    12901 gctgaaaact accccaccga acccaacggc cctctttagg gccacaaatc ttataatcct
    12961 atgttattaa ccaagtgaat gaataatttt caattatact ctttcataat tttcaggttt
    13021 tgcattttcc aagttcagtt aatttgagat taacccagta ttcattttct aaaagtcatt
    13081 ctttcagaga gagaaattca aagcatcttc ggaattctat ctttcaaatt gtagatggga
    13141 ttcttcgaat atttccaaaa acttgtattt ccgtagttta tgaagttgca cagagaaagg
    13201 gaagttccta tactatttga agaataggaa cttcatgcct gcaggacgcg aagagcaagc
    13261 ttggatcgac gagagcagcg cgactggatc tgtcgcccgt ctcaaacgca accctccggc
    13321 ggtcgcatat cattcaggac gagcctcaga ctccagcgta actggactgc aatcaactca
    13381 ctggctcacc ttccggtcca cgatcagcta gaatcaagct gactagataa actggccgtc
    13441 gttttacacg gg
//
