LOCUS       sequence-306069-       11235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniMos dual component RMCE landing site vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11235)
  AUTHORS   Nonet ML
  TITLE     Efficient Transgenesis in Caenorhabditis elegans Using Flp 
            Recombinase-Mediated Cassette Exchange.
  JOURNAL   Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 
            10.1534/genetics.120.303388.
  PUBMED    32513816
REFERENCE   2  (bases 1 to 11235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2020 Jun 8. pii: genetics.120.303388. doi: 
            10.1534/genetics.120.303388."
COMMENT     Sequence Label: pNM3649
FEATURES             Location/Qualifiers
     source          1..11235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     283..299
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     283..299
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    319..339
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(397..430)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             440..469
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(2286..2609)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    4527..4551
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4554..5579
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4554..5579
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(5580..5604)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5957..5990)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6616..6649
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(6696..6724)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7472..7492
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(8398..8431)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(8475..8491)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8476..8492)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(8508..8528)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8536..8553)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8536..8552)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(8558..8569)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9239..9261
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9299..9317)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9385..9489
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9490..9558,9559..10350)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9708..9727)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10521..11109
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11010..11029
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcaccgtacg tctcgaggaa ttctatcagg tgtacaagta tgaaatgtcg tttcgaacat
       61 atagatgtct cgcaaacgta aatatttatc gattgtcata aaactttgac cttgtgaagt
      121 gtcaaccttg actgtcgaac caccatagtt tggcgcgaat tgagcgtcat aattgtttac
      181 tctcagtgca gtcaacatgt cgagtttcgt gccgaataaa gagcaaacgc ggacagtatt
      241 aattttctgt tttcatttga agaaaacagc tgcggccgga tccaggaaac agctatgacc
      301 atgattacgc caagctatca actttgtata gaaaagttga gctccaccgc ggtggcggcc
      361 gctctagaac taggctgttt cgaaggtaag tttaaaataa cttcgtatag catacattat
      421 acgaagttat tttcagggag agcaaaagct tatctccgag gaggacctct aaggatgatc
      481 gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa tatttgtttt ttttcctatt
      541 atttatttat tctctttgtg ttttttttct tgctttctaa aaaattaatt caatccaaat
      601 ctaaacattt ttttttctct ttccgtctcc caattcgtat tccgctcctc tcatctgaac
      661 acaatgtgca agtttattta tcttctcgct ttcatttcat taggacgtgg ggggaattgg
      721 tggaaggggg aaacacacaa aaggatgatg gaaatgaaat aaggacacac aatatgcaac
      781 aacattcaat tcagaaatat ggaggaaggt ttaaaagaaa acataaaaat atatagagga
      841 ggaaggaaac tagctgccct cgattgtcta tcttccattc tcaccaagtt tcaacaactt
      901 tattcagtca ttcttctctg gttcatttcc caaccaatct tcccgaataa agcccatctg
      961 tcctttcggc cacattcctc acctacatcc gtactcctta tctcccgttc ttatccagtc
     1021 ttttatcgtc tctgcgtctc gcacactgtc tcgtcggtaa tgtaaaaaaa aaacaaaaaa
     1081 aacttgaaaa cttgcggaaa gatttcctaa taaaaggaga cgaatccggt aggaaatcga
     1141 caagtctctg cttctcgaag actaggtgag ttacatttct ttggccgaat tattttgaaa
     1201 tcctaaattt gataatttca gagatgtctg taaaacttgc gtgttatgtg acggcttcgg
     1261 tcactgtcgc cactcttatg gtatgcttca tgaccatgtc aaccatctac tcggaggttg
     1321 atggattcag agagaagctt gataccgaga tgaatgtgtt cagagtgagt taaattatat
     1381 tttgaatttt aaataatttt taattttcta gcaatctacc aatggattgt ggaaggacat
     1441 agttgtcatc ggaagatcta gcaagcgtgt ccgttgtcaa tatgaagaga ccaacgctac
     1501 cccaactcca catgctgatg gatccccatc tgctccacca ggtcaaccac cagcagttcc
     1561 accagtcttc aaccagccaa agactccaaa tggagccaat ggaaatggac caacctgcaa
     1621 ctgcaatgct gataacaagt gcccagctgg accatccgga ccaaagggag ttccaggagt
     1681 tccaggactc gacggagttc caggacttga cggtgttcca ggagttggag ctgatgatat
     1741 cgctccacaa cgcgagtctg tcggatgctt cacttgccca caaggaccag ttggaccacc
     1801 aggagctctt ggaagaccag gaccacgtgg acttccagga ccaagaggac aaaatggaaa
     1861 cccaggaaga gatggacaac caggacatcc aggagagcaa ggatcatccg gtcaaatcgg
     1921 aaagatcgga gagccaggac caccaggaga gaagggacgc gacgccgagc atccaatcgg
     1981 aagaccagga ccaaagggac caagaggaga tcaaggacca acaggaccag ctggacagaa
     2041 cggtcttcac ggaccaccag gagagccagg aaccgttgga ccagaaggac catctggaaa
     2101 gcaaggacgt caaggaccag acggaaccca gggagagact ggaccagacg gaagaccagg
     2161 aaaggatgcc gagtactgcc agtgcccaga caagtctcca ccatcagagg ctgtcaacgc
     2221 caaccgtgga tacagaaata tctaaattgt tggtgttttc taataaaata tttgagattc
     2281 agtgagactt ttttcttggc ggcacaataa agtttcctct ttttgttggg tcactcaacg
     2341 aacacaatac aaatatttga gcgctcgcat ttttctcaac ctttcgggtt taaagcactt
     2401 caaaatggaa ttgaagagag attgtgaaag tacatcagac agaatcacat tttgatagca
     2461 ttcacttcac tcagatgcaa acttttcagt gactataaaa agtcataaaa taataacatt
     2521 aaaaaagcaa ataaattagc gagagaattt tttgacaaaa agaaagaaga gtgatagaga
     2581 agaagggaat gcttgaaagg attttgcatt tatcttaatc accgtcttcg agaagacgaa
     2641 ccatggcgcc agtttcagaa tccaagtttc taatatagtt catcacaatg ttgacgttgg
     2701 tccaacctcc ggcttgcata atctctggga tggaaactcc agcgcgagcc atgtcacggg
     2761 cagctccgac acgggcggaa tgtccagacc aagccaagta acgttgtccg gaatcatcct
     2821 tggctccgta gatgagacgg tgggtagctt cgaagattcc tgaaaattta ttttcagctt
     2881 attcttagct aatatgttgt ttaaacttac cttcaagagc tcgagtcgag agttgtgatg
     2941 ttgcactcgg ggcagcaact ccgttcttgc gaacacgaca gaacaggtag ttgtttggat
     3001 catcggcgac tccggaaacc gaaatccagc gctcaacgag cttggtaact ccaagggaga
     3061 gcgctttttc gactccggca gtcgagacca gcgtttttgt acgtccgatg tggatgagca
     3121 ttcttcctcc gtctgtgcga ctaatatcct tgacgcgaat gcgggcaatt tcggcgatac
     3181 ggagaagtgt gttgtaagca attccgagga aggcgaggtt tcggatgtcc tggcaacgat
     3241 cggaattttc cataagagaa cggacctgat cgaagtcggt tctgaaaatt tcgtcaagtc
     3301 ttaggattga atacattaat atttaaactt acctttcgaa ggcgagggct tgcttggctc
     3361 tctctccagc atcaacattt tccttgcgga tacgtctcat tacgagagat acggcattag
     3421 aatctgatgg gcgtggaagt ccagatcggc gatggagcat attgagctgt ccgagatgtt
     3481 gttggatcgt cttcaccgcc aatccacgag cttggaggta gagcaagtag tcacgaacgt
     3541 cttctggctc ggctgggaac cacttgcggt tgttaagctt gcaccaggca gcccaagaac
     3601 ggcacactga gagaagcatc ttccaggtgt gctcggagaa ggcttgtctg tcacggaaca
     3661 tatccatgag gttcttgcga acttcatcac ttgtcgcgtc aactggaagt gctggaagat
     3721 tttgatgaac agtgagaaga tttgacatat tttcgaagtt ttttagatgc actagaacaa
     3781 agcgtgttgg cttcctctga gcccgctttc cttatatacc cgcattctgc agccttacag
     3841 aatgttctag aaggtcctag atgcattcgt ttgaaaatac tcccggtggg tgcaaagaga
     3901 cgcagacgga aaatgtatct gggtctcttt attgtgtaca ctacttttcc atgtaccgaa
     3961 tgtgagtcgc cctccttttg caacaagcag ctcgaatgtt ctagaaaaag gtggaaaata
     4021 gtataaatac cgttgaaaat aaataccgaa caacatttgc tctaattgtg aaattagaaa
     4081 tcttcaaact ataatcatgt cactttacca ctatttccgt ccagctcaac gttccgtttt
     4141 tggtgatcat ttttgctttc gtcgtaaatc tacacacgcg tctcttccgt gcgagagtcc
     4201 aagccagcag ccaaattcgt tgactgagta ttcaacgttt atacgttgtc ggcaacgaga
     4261 aataggaaaa tgcatcggga aatgttcttt tttcgatttt ttccaaggtt ttgacaaatt
     4321 ttaccacgaa ttttgctatg ttttcaatta aaaaatatgt tattcaactg tttctatgag
     4381 gaaaataagg ctttgcatgt aattttctta ttcagcataa tttttaatta atttgaattt
     4441 tctgtcctaa cgtttatttt gttttcttgg ttatgactga tctgaaatta atttttgaat
     4501 tttaaggtaa tatgtcaggc ggtgccgcaa gtttgtacaa aaaagcaggc tccatgaaaa
     4561 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     4621 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     4681 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     4741 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     4801 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     4861 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     4921 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     4981 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     5041 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     5101 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     5161 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     5221 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     5281 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     5341 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     5401 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     5461 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     5521 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga
     5581 cccagctttc ttgtacaaag tgggtccaat tactcttcaa catccctaca tgctctttct
     5641 ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt
     5701 ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta
     5761 attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc
     5821 ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt
     5881 ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt
     5941 atgaccgcaa tttttaataa cttcgtatag catacattat acgaagttat tttcagggtg
     6001 ggagaaaatg cggagagaag aacccagcat cttaataaaa ttatataatt ttgaataaaa
     6061 tcaaattaaa aataagatta attgaaacac aacaatagaa tgcagaaaat tactcataag
     6121 aaataattta ttttgaaaga agttgtgtcg atttacgata aaaactacac gcaaacgagg
     6181 ctccgcgttt acaccgtttg gcaatggagc gcgcttgcta ttagatttta ggccagctca
     6241 acgaaagttt aatttatttt tcttcttttt ttccattttt ttgagtaaaa tatcgaattt
     6301 tttgtattaa ttctgtaaat tttataattt gcaactattt tggcgtattc tgcttgtttt
     6361 tatcagcgaa tttttatgca tttcagtcaa aagtttgagc aggaaaaatg acatttagga
     6421 ctttgtaaaa ttgtattctt aaatttgctt gaaaacctac gtgtacaatt gattttattg
     6481 aatttttctt acgtttcccg ttgtatctta ttgtatttaa ctttcatcat aaaataattt
     6541 atttccaggt aaaatatccg acgctctcgt ggatccccgg gattggctca gaaaaaaagg
     6601 tacgagcagg cttacgaagt tcctattctc tagaaagtat aggaacttcg gtaccggtag
     6661 aaaaaatgag taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
     6721 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
     6781 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatgggtaa
     6841 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagcca acacttgtca
     6901 ctactttctg ttatggtgtt caatgcttct cgagataccc agatcatatg aaacggcatg
     6961 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
     7021 atgacgggaa ctacaagaca cgtaagttta aacagttcgg tactaactaa ccatacatat
     7081 ttaaattttc aggtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
     7141 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
     7201 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttgtaa
     7261 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagaac ttcaaaatta
     7321 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
     7381 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
     7441 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
     7501 ggattacaca tggcatggat gaactataca aatgcccggg ggatcggtgg agctccaccg
     7561 gtggcggccg ctctagaact agtccaccaa agccatctgc caagggagcc aagaaggccg
     7621 ccaagaccgt cgttgccaag ccaaaggacg gaaagaagag acgtcatgcc cgcaaggaat
     7681 cgtactccgt ctacatctac cgtgttctca agcaagttca cccagacacc ggagtctcct
     7741 ccaaggccat gtctatcatg aactccttcg tcaacgatgt attcgaacgc atcgcttcgg
     7801 aagcttcccg tcttgctcat tacaacaaac gctcaacgat ctcatcccgc gaaattcaaa
     7861 ccgctgtccg tttgattctc ccaggagaac ttgccaagca cgccgtgtct gagggaacca
     7921 aggccgtcac caagtacact tccagcaagt aaactagtgg atctgcaacg cgtggagcta
     7981 gcgcatcgcg aagctccgca tcggccgctg tcatcagatc gccatctcgc gcccgtgcct
     8041 ctgacttcta agtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc
     8101 accccctatt tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt
     8161 ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa
     8221 aagtcgaaaa aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca
     8281 caatgttctg tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat
     8341 agaaaaaacc gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcggaa
     8401 gttcctatac tatttgaaga ataggaactt catgtaacgg tcttctgcct gcaggaattc
     8461 gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccaa ctttattata
     8521 catagttgat aattcactgg ccgtcgtttt acatggcacg cccttctatt acagggtttc
     8581 tgataacaaa gggtgccgga tctgcggctt actcaccaga cctggcccca tccgattacc
     8641 acctattcgc ttcgatggga cacgcactcg ctgagcagcg cttcgattct tacgaaagtg
     8701 tgaaaaaatg gctcgatgaa tggttcgccg caaaagacga tgagttctac tggcgtggaa
     8761 tccacaaatt gcccgagaga tgggaaaaat gtgtagctag cgacggcaaa tactttgaat
     8821 aaatgatttt ttctttttcc acaaaattta acgtgttttt tgatttaaaa aaaacgacat
     8881 ttcatacttg tacacctgat atctagatac ctaggtgagc tctggtaccc ccctggcgtt
     8941 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     9001 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     9061 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     9121 gcaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     9181 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     9241 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     9301 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     9361 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     9421 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     9481 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     9541 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     9601 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     9661 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     9721 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     9781 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     9841 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     9901 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     9961 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
    10021 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
    10081 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
    10141 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
    10201 cgtgggtctc gcggtatcat tgcagcactg gggtcagatg gtaagccctc ccgtatcgta
    10261 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    10321 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    10381 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    10441 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    10501 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    10561 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    10621 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
    10681 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    10741 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    10801 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    10861 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    10921 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    10981 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    11041 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    11101 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggtcttttg ctggcctttt
    11161 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    11221 gagtgagctg atacc
//
