LOCUS       sequence-306070-       14829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniMos single component RMCE landing site vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14829)
  AUTHORS   Nonet ML
  TITLE     Efficient Transgenesis in Caenorhabditis elegans Using Flp 
            Recombinase-Mediated Cassette Exchange.
  JOURNAL   Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 
            10.1534/genetics.120.303388.
  PUBMED    32513816
REFERENCE   2  (bases 1 to 14829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2020 Jun 8. pii: genetics.120.303388. doi: 
            10.1534/genetics.120.303388."
COMMENT     Sequence Label: pNM3689
FEATURES             Location/Qualifiers
     source          1..14829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     283..299
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     283..299
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    319..339
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(397..430)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             440..469
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(2286..2609)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    4527..4551
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4554..5579
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4554..5579
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(5580..5604)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5957..5990)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    10210..10243
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(10290..10318)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     11066..11086
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(11992..12025)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(12069..12085)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12070..12086)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(12102..12122)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(12130..12147)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12130..12146)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(12152..12163)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     12833..12855
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12893..12911)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12979..13083
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13084..13152,13153..13944)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13302..13321)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14115..14703
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14604..14623
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcaccgtacg tctcgaggaa ttctatcagg tgtacaagta tgaaatgtcg tttcgaacat
       61 atagatgtct cgcaaacgta aatatttatc gattgtcata aaactttgac cttgtgaagt
      121 gtcaaccttg actgtcgaac caccatagtt tggcgcgaat tgagcgtcat aattgtttac
      181 tctcagtgca gtcaacatgt cgagtttcgt gccgaataaa gagcaaacgc ggacagtatt
      241 aattttctgt tttcatttga agaaaacagc tgcggccgga tccaggaaac agctatgacc
      301 atgattacgc caagctatca actttgtata gaaaagttga gctccaccgc ggtggcggcc
      361 gctctagaac taggctgttt cgaaggtaag tttaaaataa cttcgtatag catacattat
      421 acgaagttat tttcagggag agcaaaagct tatctccgag gaggacctct aaggatgatc
      481 gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa tatttgtttt ttttcctatt
      541 atttatttat tctctttgtg ttttttttct tgctttctaa aaaattaatt caatccaaat
      601 ctaaacattt ttttttctct ttccgtctcc caattcgtat tccgctcctc tcatctgaac
      661 acaatgtgca agtttattta tcttctcgct ttcatttcat taggacgtgg ggggaattgg
      721 tggaaggggg aaacacacaa aaggatgatg gaaatgaaat aaggacacac aatatgcaac
      781 aacattcaat tcagaaatat ggaggaaggt ttaaaagaaa acataaaaat atatagagga
      841 ggaaggaaac tagctgccct cgattgtcta tcttccattc tcaccaagtt tcaacaactt
      901 tattcagtca ttcttctctg gttcatttcc caaccaatct tcccgaataa agcccatctg
      961 tcctttcggc cacattcctc acctacatcc gtactcctta tctcccgttc ttatccagtc
     1021 ttttatcgtc tctgcgtctc gcacactgtc tcgtcggtaa tgtaaaaaaa aaacaaaaaa
     1081 aacttgaaaa cttgcggaaa gatttcctaa taaaaggaga cgaatccggt aggaaatcga
     1141 caagtctctg cttctcgaag actaggtgag ttacatttct ttggccgaat tattttgaaa
     1201 tcctaaattt gataatttca gagatgtctg taaaacttgc gtgttatgta acggcttcgg
     1261 tcactgtcgc cactcttatg gtatgcttca tgaccatgtc aaccatctac tcggaggttg
     1321 atggattcag agagaagctt gataccgaga tgaatgtgtt cagagtgagt taaattatat
     1381 tttgaatttt aaataatttt taattttcta gcaatctacc aatggattgt ggaaggacat
     1441 agttgtcatc ggaagatcta gcaagcgtgt ccgttgtcaa tatgaagaga ccaacgctac
     1501 cccaactcca catgctgatg gatccccatc tgctccacca ggtcaaccac cagcagttcc
     1561 accagtcttc aaccagccaa agactccaaa tggagccaat ggaaatggac caacctgcaa
     1621 ctgcaatgct gataacaagt gcccagctgg accatccgga ccaaagggag ttccaggagt
     1681 tccaggactc gacggagttc caggacttga cggtgttcca ggagttggag ctgatgatat
     1741 cgctccacaa cgcgagtctg tcggatgctt cacttgccca caaggaccag ttggaccacc
     1801 aggagctctt ggaagaccag gaccacgtgg acttccagga ccaagaggac aaaatggaaa
     1861 cccaggaaga gatggacaac caggacatcc aggagagcaa ggatcatccg gtcaaatcgg
     1921 aaagatcgga gagccaggac caccaggaga gaagggacgc gacgccgagc atccaatcgg
     1981 aagaccagga ccaaagggac caagaggaga tcaaggacca acaggaccag ctggacagaa
     2041 cggtcttcac ggaccaccag gagagccagg aaccgttgga ccagaaggac catctggaaa
     2101 gcaaggacgt caaggaccag acggaaccca gggagagact ggaccagacg gaagaccagg
     2161 aaaggatgcc gagtactgcc agtgcccaga caagtctcca ccatcagagg ctgtcaacgc
     2221 caaccgtgga tacagaaata tctaaattgt tggtgttttc taataaaata tttgagattc
     2281 agtgagactt ttttcttggc ggcacaataa agtttcctct ttttgttggg tcactcaacg
     2341 aacacaatac aaatatttga gcgctcgcat ttttctcaac ctttcgggtt taaagcactt
     2401 caaaatggaa ttgaagagag attgtgaaag tacatcagac agaatcacat tttgatagca
     2461 ttcacttcac tcagatgcaa acttttcagt gactataaaa agtcataaaa taataacatt
     2521 aaaaaagcaa ataaattagc gagagaattt tttgacaaaa agaaagaaga gtgatagaga
     2581 agaagggaat gcttgaaagg attttgcatt tatcttaatc accgtcttcg agaagacgaa
     2641 ccatggcgcc agtttcagaa tccaagtttc taatatagtt catcacaatg ttgacgttgg
     2701 tccaacctcc ggcttgcata atctctggga tggaaactcc agcgcgagcc atgtcacggg
     2761 cagctccgac acgggcggaa tgtccagacc aagccaagta acgttgtccg gaatcatcct
     2821 tggctccgta gatgagacgg tgggtagctt cgaagattcc tgaaaattta ttttcagctt
     2881 attcttagct aatatgttgt ttaaacttac cttcaagagc tcgagtcgag agttgtgatg
     2941 ttgcactcgg ggcagcaact ccgttcttgc gaacacgaca gaacaggtag ttgtttggat
     3001 catcggcgac tccggaaacc gaaatccagc gctcaacgag cttggtaact ccaagggaga
     3061 gcgctttttc gactccggca gtcgagacca gcgtttttgt acgtccgatg tggatgagca
     3121 ttcttcctcc gtctgtgcga ctaatatcct tgacgcgaat gcgggcaatt tcggcgatac
     3181 ggagaagtgt gttgtaagca attccgagga aggcgaggtt tcggatgtcc tggcaacgat
     3241 cggaattttc cataagagaa cggacctgat cgaagtcggt tctgaaaatt tcgtcaagtc
     3301 ttaggattga atacattaat atttaaactt acctttcgaa ggcgagggct tgcttggctc
     3361 tctctccagc atcaacattt tccttgcgga tacgtctcat tacgagagat acggcattag
     3421 aatctgatgg gcgtggaagt ccagatcggc gatggagcat attgagctgt ccgagatgtt
     3481 gttggatcgt cttcaccgcc aatccacgag cttggaggta gagcaagtag tcacgaacgt
     3541 cttctggctc ggctgggaac cacttgcggt tgttaagctt gcaccaggca gcccaagaac
     3601 ggcacactga gagaagcatc ttccaggtgt gctcggagaa ggcttgtctg tcacggaaca
     3661 tatccatgag gttcttgcga acttcatcac ttgtcgcgtc aactggaagt gctggaagat
     3721 tttgatgaac agtgagaaga tttgacatat tttcgaagtt ttttagatgc actagaacaa
     3781 agcgtgttgg cttcctctga gcccgctttc cttatatacc cgcattctgc agccttacag
     3841 aatgttctag aaggtcctag atgcattcgt ttgaaaatac tcccggtggg tgcaaagaga
     3901 cgcagacgga aaatgtatct gggtctcttt attgtgtaca ctacttttcc atgtaccgaa
     3961 tgtgagtcgc cctccttttg caacaagcag ctcgaatgtt ctagaaaaag gtggaaaata
     4021 gtataaatac cgttgaaaat aaataccgaa caacatttgc tctaattgtg aaattagaaa
     4081 tcttcaaact ataatcatgt cactttacca ctatttccgt ccagctcaac gttccgtttt
     4141 tggtgatcat ttttgctttc gtcgtaaatc tacacacgcg tctcttccgt gcgagagtcc
     4201 aagccagcag ccaaattcgt tgactgagta ttcaacgttt atacgttgtc ggcaacgaga
     4261 aataggaaaa tgcatcggga aatgttcttt tttcgatttt ttccaaggtt ttgacaaatt
     4321 ttaccacgaa ttttgctatg ttttcaatta aaaaatatgt tattcaactg tttctatgag
     4381 gaaaataagg ctttgcatgt aattttctta ttcagcataa tttttaatta atttgaattt
     4441 tctgtcctaa cgtttatttt gttttcttgg ttatgactga tctgaaatta atttttgaat
     4501 tttaaggtaa tatgtcaggc ggtgccgcaa gtttgtacaa aaaagcaggc tccatgaaaa
     4561 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     4621 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     4681 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     4741 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     4801 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     4861 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     4921 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     4981 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     5041 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     5101 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     5161 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     5221 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     5281 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     5341 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     5401 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     5461 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     5521 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga
     5581 cccagctttc ttgtacaaag tgggtccaat tactcttcaa catccctaca tgctctttct
     5641 ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt
     5701 ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta
     5761 attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc
     5821 ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt
     5881 ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt
     5941 atgaccgcaa tttttaataa cttcgtatag catacattat acgaagttat tttcagggtg
     6001 ggagaaaatg cggagcatgc ggccgcatat cagtttttaa aaaattaaac cataaaacaa
     6061 ataatataac ccaattttta catcaaacca caagaaaaaa atacatttgg gcccacggat
     6121 aaagaaatta aaaaaataca ttttttaaag gcgcaccgaa ttaaaattca tttgggtctt
     6181 accgcgtata ccgtactccg tttgtttgat catttttgtc agcgctggcg gttgtttttt
     6241 catttcattt ctgcttcaaa gacgttttct cgaataattt ttcgtttatt ctctttttta
     6301 aaattaattt ctagccgtaa atgttataaa ttcacccatt taacgcaaat ttcatggtaa
     6361 tctcatggaa aaatgcagtt tctttgttaa agaaagctta aatagcaaaa attccccgac
     6421 tttccccaaa atcctgctcg attttccgtt ttctcattgt attctctctt aattaatttt
     6481 atcgataatc aattgaatgt ttcagacaga gaggtaccaa aaatgccaca attcgacatt
     6541 ctctgtaaga caccaccaaa agtcctcgtc cgtcaattcg tcgagcgttt cgagcgtcca
     6601 tccggagaga agatcgccct ctgcgccgcc gagctcacct acctctgctg gatgatcacc
     6661 cacaacggaa ccgccatcaa gcgtgccacc ttcatgtcct acaacaccat catctccaac
     6721 tccctctcct tcgacatcgt caacaagtcc ctccaattca agtacaagac ccaaaaggcc
     6781 accatcctcg aggcctccct caaggtaagt ttaaacatat atatactaac taaccctgat
     6841 tatttaaatt ttcagaagct catcccagcc tgggagttca ccatcatccc atactacgga
     6901 caaaagcacc aatccgacat caccgacatc gtctcctccc tccaactcca attcgagtcc
     6961 tccgaggagg ccgacaaggg aaactcccac tccaagaaga tgctcaaggc cctcctctcc
     7021 gagggagagt ccatctggga gatcaccgag aagatcctca actccttcga gtacacctcc
     7081 cgtttcacca agaccaagac cctctaccaa ttcctcttcc tcgccacctt catcaactgc
     7141 ggacgtttct ccgacatcaa gaacgtcgac ccaaagtcct tcaagctcgt ccaaaacaag
     7201 tacctcggag tcatcatcca atgcctcgtc accgagacca agacctccgt ctcccgtcac
     7261 atctacttct tctccgcccg tggacgtatc gacccactcg tctacctcga cgagttcctc
     7321 cgtaactccg agccagtcct caagcgtgtc aaccgtaccg gaaactcctc ctccaacaag
     7381 caagagtacc aactcctcaa ggacaacctc gtccgttcct acaacaaggc cctcaagaag
     7441 aacgccccat actccatctt cgccatcaag aacggaccaa agtcccacat cggacgtcac
     7501 ctcatgacct ccttcctctc catgaaggta agtttaaaca tgattttact aactaactaa
     7561 tctgatttaa attttcaggg actcaccgag ctcaccaacg tcgtcggaaa ctggtccgac
     7621 aagcgtgcct ccgccgtcgc ccgtaccacc tacacccacc aaatcaccgc catcccagac
     7681 cactacttcg ccctcgtctc ccgttactac gcctacgacc caatctccaa ggagatgatc
     7741 gccctcaagg acgagaccaa cccaatcgag gagtggcaac acatcgagca actcaaggga
     7801 tccgccgagg gatccatccg ttacccagcc tggaacggaa tcatctccca agaggtcctc
     7861 gactacctct cctcctacat caaccgtcgt atctaacgcg tgctgtctca tcctactttc
     7921 acctagttaa ctgcttgtct taaaatctat gcttctcttt agtatctaaa attttcctag
     7981 aagcttacaa gtatataaat ggtctcttct caataaaggt tgtatattta ttcatcttat
     8041 tgaatctgcc atttcctcgt ttttgcgagt ttatatacct tccaattttc tttctattgt
     8101 attttcaact tctaatttta attcagggaa actgcttcaa cgcatcatgg tgtcgaaggg
     8161 agaagaggat aacatggctt cactcccagc tacacacgaa ctccacatct tcggatcgat
     8221 caacggagtg gatttcgata tggtcggaca aggtaagttt aaacatatat atactaacta
     8281 accctgatta tttaaatttt caggaactgg aaacccaaac gatggatacg aggaactcaa
     8341 cctcaagtcg acaaagggag atctgcaatt ctcgccatgg attctcgtgc cacacatcgg
     8401 atacggattc caccaatacc tcccataccc aggtaagttt aaactgagtt ctactaacta
     8461 acgagtaata tttaaatttt cagatggaat gtcaccattc caagctgcca tggtggatgg
     8521 atcgggatac caagttcacc gaacaatgca attcgaggat ggagcctcgc tcacagtgaa
     8581 ctaccgatac acatacgagg gatcgcacat caaggtaagt ttaaacagtt cggtactaac
     8641 taaccataca tatttaaatt ttcagggaga ggctcaagtt aagggaacag gattcccagc
     8701 tgatggacca gtgatgacaa actcactcac agctgctgat tggtgccgat cgaaaaagac
     8761 atacccaaat gataagacaa tcatctcgac attcaagtgg tcgtacacta ctggaaacgg
     8821 aaagcgatac cgatcgacag cccgaacaac atacacattc gctaagccaa tggccgccaa
     8881 ctacctcaag gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca
     8941 gaatcaacca atgtacgtgt tccgaaagac agaactcaag cactcaaaga cagagctgaa
     9001 cttcaaagag tggcaaaagg ccttcacaga tgtgatggga atggatgaac tctacaagta
     9061 acgcgtatgt atctgcatat ccttcaaatg ttgtccatat tgtatcagta aattataatg
     9121 cccccttttt atatattctc cttatcatgt aaatgttcta ttgatttttt gctggtgtaa
     9181 aacgttttta tagtaaatcc tctccacagt gaatttgtta aattgtatca aaacaagaca
     9241 ttgattaaat ttgttcgaga cttgtggatt gtaaaatatt ttgcagaaaa tggcaatatt
     9301 catttagaaa cattacttcc ataataggaa aaactattta tgagttttca tgtgcctatg
     9361 caagttccca ctctgtgtga atttcatcat acaaattgag caaacataag gtttatgtcc
     9421 agtatgggtt ctcagatgtt ttgtaagagt tgagctgtcg gtgaatgcct tctcacattg
     9481 agcacatgga tacggtctct ctccagtgtg tgttctgaaa taatgaaaaa tagtcttgca
     9541 atatgtttct actattcgag aaaaaatgta attttttttc ttatggtaac ccggctttcc
     9601 ccagcggaga gaagaaccca gcatcttaat aaaattatat aattttgaat aaaatcaaat
     9661 taaaaataag attaattgaa acacaacaat agaatgcaga aaattactca taagaaataa
     9721 tttattttga aagaagttgt gtcgatttac gataaaaact acacgcaaac gaggctccgc
     9781 gtttacaccg tttggcaatg gagcgcgctt gctattagat tttaggccag ctcaacgaaa
     9841 gtttaattta tttttcttct ttttttccat ttttttgagt aaaatatcga attttttgta
     9901 ttaattctgt aaattttata atttgcaact attttggcgt attctgcttg tttttatcag
     9961 cgaattttta tgcatttcag tcaaaagttt gagcaggaaa aatgacattt aggactttgt
    10021 aaaattgtat tcttaaattt gcttgaaaac ctacgtgtac aattgatttt attgaatttt
    10081 tcttacgttt cccgttgtat cttattgtat ttaactttca tcataaaata atttatttcc
    10141 aggtaaaata tccgacgctc tcgtggatcc ccgggattgg ctcagaaaaa aaggtacgag
    10201 caggcttacg aagttcctat tctctagaaa gtataggaac ttcggtaccg gtagaaaaaa
    10261 tgagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
    10321 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
    10381 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg gtaagtttaa
    10441 acatatatat actaactaac cctgattatt taaattttca gccaacactt gtcactactt
    10501 tctgttatgg tgttcaatgc ttctcgagat acccagatca tatgaaacgg catgactttt
    10561 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
    10621 ggaactacaa gacacgtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat
    10681 tttcaggtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
    10741 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
    10801 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt gtaagtttaa
    10861 acatgatttt actaactaac taatctgatt taaattttca gaacttcaaa attagacaca
    10921 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
    10981 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
    11041 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
    11101 cacatggcat ggatgaacta tacaaatgcc cgggggatcg gtggagctcc accggtggcg
    11161 gccgctctag aactagtcca ccaaagccat ctgccaaggg agccaagaag gccgccaaga
    11221 ccgtcgttgc caagccaaag gacggaaaga agagacgtca tgcccgcaag gaatcgtact
    11281 ccgtctacat ctaccgtgtt ctcaagcaag ttcacccaga caccggagtc tcctccaagg
    11341 ccatgtctat catgaactcc ttcgtcaacg atgtattcga acgcatcgct tcggaagctt
    11401 cccgtcttgc tcattacaac aaacgctcaa cgatctcatc ccgcgaaatt caaaccgctg
    11461 tccgtttgat tctcccagga gaacttgcca agcacgccgt gtctgaggga accaaggccg
    11521 tcaccaagta cacttccagc aagtaaacta gtggatctgc aacgcgtgga gctagcgcat
    11581 cgcgaagctc cgcatcggcc gctgtcatca gatcgccatc tcgcgcccgt gcctctgact
    11641 tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc
    11701 tatttttgtt attatcaaaa aacttctctt aatttctttg ttttttagct tcttttaagt
    11761 cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg
    11821 aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt
    11881 tctgtgtaca cttcttatgt tttttacttc tgataaattt ttttgaaaca tcatagaaaa
    11941 aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc ggaagttcct
    12001 atactatttg aagaatagga acttcatgta acggtcttct gcctgcagga attcgatatc
    12061 aagcttatcg ataccgtcga cctcgagggg gggcccggta ccaactttat tatacatagt
    12121 tgataattca ctggccgtcg ttttacatgg cacgcccttc tattacaggg tttctgataa
    12181 caaagggtgc cggatctgcg gcttactcac cagacctggc cccatccgat taccacctat
    12241 tcgcttcgat gggacacgca ctcgctgagc agcgcttcga ttcttacgaa agtgtgaaaa
    12301 aatggctcga tgaatggttc gccgcaaaag acgatgagtt ctactggcgt ggaatccaca
    12361 aattgcccga gagatgggaa aaatgtgtag ctagcgacgg caaatacttt gaataaatga
    12421 ttttttcttt ttccacaaaa tttaacgtgt tttttgattt aaaaaaaacg acatttcata
    12481 cttgtacacc tgatatctag atacctaggt gagctctggt acccccctgg cgttacccaa
    12541 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
    12601 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
    12661 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtgcaatc
    12721 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
    12781 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
    12841 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
    12901 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
    12961 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    13021 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    13081 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    13141 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    13201 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    13261 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    13321 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    13381 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    13441 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    13501 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    13561 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    13621 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    13681 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    13741 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    13801 tctcgcggta tcattgcagc actggggtca gatggtaagc cctcccgtat cgtagttatc
    13861 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    13921 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    13981 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    14041 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    14101 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    14161 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    14221 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    14281 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    14341 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    14401 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    14461 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    14521 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    14581 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    14641 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    14701 aaaaacgcca gcaacgcggc ctttttacgg ttcctggtct tttgctggcc ttttgctcac
    14761 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    14821 gctgatacc
//
