LOCUS       sequence-306144-        8668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knocking down of human YY1 through shRNA and amiRNA targeting YY1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Pan G, Diamanti K, Cavalli M, Lara Gutierrez A, Komorowski J, 
            Wadelius C
  TITLE     Multifaceted regulation of hepatic lipid metabolism by YY1.
  JOURNAL   Life Sci Alliance. 2021 Jun 7;4(7). pii: 4/7/e202000928. doi: 
            10.26508/lsa.202000928. Print 2021 Jul.
  PUBMED    34099540
REFERENCE   2  (bases 1 to 8668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-06-7"; volume: "4(7). pii: 4/7/e202000928. 
            doi"; pages: " 10.26508/lsa.202000928. Print 2021 Jul"
COMMENT     Sequence Label: pBMN-AS-YY1
FEATURES             Location/Qualifiers
     source          1..8668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..52)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(206..794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(965..1756,1757..1825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1588..1607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1826..1930)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1998..2016
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2054..2076)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2176..2195
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2389..2411
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2403..2420
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2404..2420
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2435..2661
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2662..2842
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2889..3014
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3507..3740
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3925..3969
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4236..4352
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4442..4645
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4596..4616
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4642..4666
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4717..4726
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4747..5409
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             5491..5544
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5560..5616
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5638..5703
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             5716..6315
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5716..5735)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6212..6232
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6877..7465
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6930..6950)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7348..7359)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7577..7791
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     7712..7731
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     LTR             7891..8071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8143..8264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8180..8199)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8234..8253
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8304..8439
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8472..8488)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8472..8488)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8485..8507)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8496..8512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8520..8526,8527..8544,8545..8550))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8565..8586
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
       61 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1921 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     1981 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     2041 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2101 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2161 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2221 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     2281 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2341 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     2401 gttgtaaaac gacggccagt gccaagctga cgcgtgtagt cttatgcaat actcttgtag
     2461 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     2521 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     2581 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     2641 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     2701 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     2761 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     2821 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     2881 cagaggagct ctctcgacgc aggactcggt ttgctgaagc gcgcacggca agaggcgagg
     2941 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     3001 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gggatgggaa aaaattcggt
     3061 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     3121 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     3181 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     3241 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     3301 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     3361 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     3421 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     3481 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     3541 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     3601 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     3661 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     3721 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     3781 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     3841 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     3901 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     3961 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     4021 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     4081 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     4141 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     4201 cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg
     4261 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     4321 ttacaaaaac aaattacaaa attcaaaatt ttatcgatac tagtattatg cccagtacat
     4381 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     4441 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     4501 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     4561 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     4621 gtgggaggtt tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     4681 tccacgctgt tttgacctcc atagaagatt ctagacgcca ccatggagag cgacgagagc
     4741 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     4801 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     4861 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     4921 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     4981 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     5041 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     5101 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     5161 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     5221 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     5281 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     5341 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     5401 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     5461 ggctccaccg gatctcgcga gggctccggg gagggcagag gaagtctgct aacatgcggt
     5521 gacgtcgagg agaatcctgg gcccgctagc ggatcaggtg ccacgaactt ctctctgtta
     5581 aagcaagcag gagacgtgga agaaaacccc ggtcccttaa ttaatggctc cggaccggtg
     5641 aaacagactt tgaattttga ccttctcaag ttggcgggag acgtggagtc caacccaggc
     5701 ccagtcgacc ccgggatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     5761 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     5821 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     5881 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     5941 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6001 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6061 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6121 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6181 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6241 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6301 aagcccggtg cctgatatcg tcgctgttat ctcattgtct gtgtgattgg gggagctgcg
     6361 gcggggagga tgctgtggtc ccttcctccg gcgttcccca cccccatccc tctccccgct
     6421 gtcagtgcgc acgcacacgc gccgcttttt atttcttttt cctggttttc ttattccatc
     6481 ttctacccac ccctcttcct ttctttcacc tttccttcct tccttcctcc tttccttcct
     6541 caggagaaag gccgatatct acgagcagaa gaagggacag attttaaatg tccatacaaa
     6601 tctgcacctg cttctgctcc cagaattcgg ctggctgagc accgtgggtc ggcgagggcc
     6661 cgccaaggaa ggagcgaccg accgagccag gcgccctccg cagacctccg cgcagcggcc
     6721 gcgggcgcga ggggaggggt ctggagctcc ctccggctgc ctgtcccgca ccggagcccg
     6781 tggggtgggg aggtgtgcag cctgtgacag acaggggctt agagatgcaa acagactcag
     6841 ggagagaaac agaagctgat tctgtgacag aagcagaatc aacctctgga ttacaaaatt
     6901 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6961 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7021 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7081 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7141 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7201 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7261 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     7321 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7381 ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg
     7441 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     7501 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7561 acgatgtcaa gaattggaac gctgacgtca tcaacccgct ccaaggaatc gcgggcccag
     7621 tgtcactagg cgggaacacc cagcgcgcgt gcgccctggc aggaagatgg ctgtgaggga
     7681 caggggagtg gcgccctgca atatttgcat gtcgctatgt gttctgggaa atcaccataa
     7741 acgtgaaatg tctttggatt tgggaatctt ataagttctg tatgagacca cttggatccg
     7801 ggagcagaag caggtgcaga tcttcctgtc agaaataata tacaaaggag aggctttttg
     7861 aattcttcga ttctgctttt tgcttctact gggtctctct ggttagacca gatctgagcc
     7921 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7981 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8041 ccctttcagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     8101 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     8161 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8221 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     8281 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     8341 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     8401 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagaccaaa
     8461 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8521 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8581 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8641 cattaatgaa tcggccaacg cgcgggga
//
