LOCUS       sequence-306154-        8880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Amber suppression for incorporating 3-aminotyrosine to proteins in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Zhang S, Ai HW
  TITLE     A general strategy to red-shift green fluorescent protein-based 
            biosensors.
  JOURNAL   Nat Chem Biol. 2020 Sep 14. pii: 10.1038/s41589-020-0641-7. doi: 
            10.1038/s41589-020-0641-7.
  PUBMED    32929278
REFERENCE   2  (bases 1 to 8880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 Sep 14. pii: 10.1038/s41589-020-0641-7. doi: 
            10.1038/s41589-020-0641-7."
COMMENT     Sequence Label: pMAH-EcaYRS
FEATURES             Location/Qualifiers
     source          1..8880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(111..201)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     promoter        complement(313..561)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        complement(850..940)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     promoter        complement(1052..1300)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     enhancer        1559..1862
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1863..2066
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2017..2037
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2063..2087
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2297..2315
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2297..2314
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2328..2347
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      2408..2417
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4320..4337)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4326..4550
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4596..5024
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4683..4702)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4893..4914
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5033..5053)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5038..5367
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5218..5353
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5280..5299
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5416..6441
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5416..6441
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6571..6692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6608..6627)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6662..6681
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6741..6757)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6741..6757)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6754..6776)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6765..6781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6789..6795,6796..6813,6814..6819))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6834..6855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6972..6989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7143..7731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7223..7242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7902..8693,8694..8762))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8525..8544
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8763..8867)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcgactcgc tagtgttcca aaaaatggtg ggggacggat tcgaaccgcc gaacccaaag
       61 ggagcggatt tagagtccgc ggcgtttggc cacttcgcta ccccaccaga gtggtctcat
      121 acagaactta taagattccc aaatccaaag acatttcacg tttatggtga tttcccagaa
      181 cacatagcga catgcaaata tgaattgatg actgcctgat ggagaaaaaa tggtggggga
      241 cggattcgaa ccgccgaacc caaagggagc ggatttagag tccgcggcgt ttggccactt
      301 cgctacccca ccggtgtttc gtcctttcca caagatatat aaagccaaga aatcgaaata
      361 ctttcaagtt acggtaagca tatgatagtc cattttaaaa cataatttta aaactgcaaa
      421 ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct ttgtgtttac
      481 agtcaaatta attctaatta tctctctaac agccttgtat cgtatatgca aatatgaagg
      541 aatcatggga aataggccct cttcctgccc gaccttcgcg atgtacgggc cagatatacg
      601 ccaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
      661 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      721 aatatgaccg ccatgttggc tcgactcgct agtgttccaa aaaatggtgg gggacggatt
      781 cgaaccgccg aacccaaagg gagcggattt agagtccgcg gcgtttggcc acttcgctac
      841 cccaccagag tggtctcata cagaacttat aagattccca aatccaaaga catttcacgt
      901 ttatggtgat ttcccagaac acatagcgac atgcaaatat gaattgatga ctgcctgatg
      961 gagaaaaaat ggtgggggac ggattcgaac cgccgaaccc aaagggagcg gatttagagt
     1021 ccgcggcgtt tggccacttc gctaccccac cggtgtttcg tcctttccac aagatatata
     1081 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac
     1141 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
     1201 ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc
     1261 gtatatgcaa atatgaagga atcatgggaa ataggccctc ttcctgcccg accttcgcga
     1321 tgtacgggcc agatatacgc caatattggc cattagccat attattcatt ggttatatag
     1381 cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca
     1441 tttatattgg ctcatgtcca atatgaccgc catgttggct cgagcattga ttattgacta
     1501 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     1561 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     1621 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     1681 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     1741 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     1801 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     1861 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
     1921 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     1981 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
     2041 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     2101 atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg
     2161 aacggtgcat tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagag
     2221 tctataggcc cacccccttg gcttcgttag aacgcggcta caattaatac ataaccttat
     2281 gtatcataca catacgattt aggtgacact atagaataac atccactttg cctttctctc
     2341 cacaggtgtc cactcccagg tccaactgca cggaagcttg cccccaggtc caactgcacg
     2401 gaagcttgcc accatggcaa gcagtaactt gattaaacaa ttgcaagagc gggggctggt
     2461 agcccaggtg acggacgagg aagcgttagc agagcgactg gcgcaaggcc cgatcgcgct
     2521 cctttgcggc ttcgatccta ccgctgacag cttgcatttg gggcatcttg ttccattgtt
     2581 atgcctgaaa cgcttccagc aggcgggcca caagccggtt gcgctggtag gcggcgcgac
     2641 gggtctgatt ggcgacccga gcttcaaagc tgccgagcgt aagctgaaca ccgaagaaac
     2701 tgttcaggag tgggtggaca aaatccgtaa gcaggttgcc ccgttcctcg atttcgactg
     2761 tggagaaaac tctgctatcg cggcgaacaa ctatgactgg ttcggcaata tgaatgtgct
     2821 gaccttcctg cgcgatattg gcaaacactt ctccgttaac cagatgatca acaaagaagc
     2881 ggttaagcag cgtctcaacc gtgaagatca ggaggttaaa aagagtaacg taacggttta
     2941 cgttgatgag aaactgaaag atataaggcc ttatggagtt tacgcaatag ttgaaggttt
     3001 aaggctcgac gaagattctt taagtcaaat gattcagcta caagaaaaga tagcccttac
     3061 atttggaaga agaaggagag aagtggccat aggaatcttc gattttgata agattaagcc
     3121 acctatttac tataaagccg ccgaaaaaac tgaaaagttt gcccccctgg gctataaaga
     3181 ggaaatgact ctagaggaga tccttgaaaa gcatgaaaag ggaagggagt atgggcacct
     3241 tataaaggat aaacaatttt atccactact tattgacagc gaggggaatg tgctctccat
     3301 gccgccaata atcaactccg agtttacggg aagagtaaca acggatacga aaaatgtctt
     3361 catagatgtc acgggatgga aacttgagaa ggtaatgctt gcccttaatg tcatggtaac
     3421 tgcattagca gagcgtggag gtaaaataag gagcgttagg gttgtctaca aggacttcga
     3481 aattgaaacc ccaggctccg cctccggccc cgcctccgcc gggatttcgt tcactgagtt
     3541 ttcctacaac ctgttgcagg gttatgactt cgcctgtctg aacaaacagt acggtgtggt
     3601 gctgtctatt ggtggttctg accagtgggg taacatcact tctggtatcg acctgacccg
     3661 tcgtctgcat cagaatcagg tgtttggcct gaccgttccg ctgatcacta aagcagatgg
     3721 caccaaattt ggtaaaactg aaggcggcgc agtctggttg gatccgaaga aaaccagccc
     3781 gtacaaattc taccagttct ggatcaacac tgcgcgggcc gacgtttacc gcttcctgaa
     3841 gttcttcacc tttatgagca ttgaagagat caacgccctg gaagaagaag ataaaaacag
     3901 cggtaaagca ccgcgcgccc agtatgtact ggcggagcag gtgactcgtc tggttcacgg
     3961 tgaagaaggt ttacaggcgg caaaacgtat taccgaatgc ctgttcagcg gttctttgag
     4021 tgcgctgagt gaagcggact tcgaacagct ggcgcaggac ggcgtaccga tggttgagat
     4081 ggaaaagggc gcagacctga tgcaggcact ggtcgattct gaactgcaac cttcccgtgg
     4141 tcaggcacgt aaaactatcg cctccaatgc catcaccatt aacggtgaaa aacagtccga
     4201 tcctgaatac ttctttaaag aagaagatcg tctgtttggt cgttttacct tactgcgtcg
     4261 cggtaaaaag aattactgtc tgatttgctg gaaagggccc gtttaaaccc gctgatcagc
     4321 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4381 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4441 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4501 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     4561 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     4621 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4681 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4741 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4801 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4861 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4921 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4981 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     5041 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5101 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     5161 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     5221 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5281 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     5341 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     5401 tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     5461 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     5521 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     5581 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     5641 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     5701 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     5761 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     5821 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     5881 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     5941 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     6001 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     6061 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     6121 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     6181 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     6241 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     6301 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     6361 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     6421 cgtccgaggg caaaggaata gcacgtgcta cgagatttcg attccaccgc cgccttctat
     6481 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6541 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     6601 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6661 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     6721 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6781 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6841 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6901 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6961 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7021 tcagctcact caaagacggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7081 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7141 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7201 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7261 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7321 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7381 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7441 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7501 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7561 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7621 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7681 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7741 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7801 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7861 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7921 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7981 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8041 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8101 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8161 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8221 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8281 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8341 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8401 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8461 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8521 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8581 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8641 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8701 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8761 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8821 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
//
