LOCUS       sequence-30616-e         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Wang HR, Zhang Y, Ozdamar B, Ogunjimi AA, Alexandrova E, Thomsen GH,
            Wrana JL
  TITLE     Regulation of cell polarity and protrusion formation by targeting 
            RhoA for degradation.
  JOURNAL   Science. 2003 Dec 5. 302(5651):1775-9.
  PUBMED    14657501
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2003 Dec 5. 302(5651):1775-9."
COMMENT     Sequence Label: pcDNA4-PKCZeta WT His tagged
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..25
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    28..46
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     56..80
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc
       61 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
      121 gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccgagc
      181 tcggatccac tagtccagtg tggtggaatt caccatgccc agcaggaccg gccccaagat
      241 ggaagggagc ggcggccgcg tccgcctcaa ggcgcattac gggggggaca tcttcatcac
      301 cagcgtggac gccgccacga ccttcgagga gctctgtgag gaagtgagag acatgtgtcg
      361 tctgcaccag cagcacccgc tcaccctcaa gtgggtggac agcgaaggtg acccttgcac
      421 ggtgtcctcc cagatggagc tggaagaggc tttccgcctg gcccgtcagt gcagggatga
      481 aggcctcatc attcatgttt tcccgagcac ccctgagcag cctggcctgc catgtccggg
      541 agaagacaaa tctatctacc gccggggagc cagaagatgg aggaagctgt accgtgccaa
      601 cggccacctc ttccaagcca agcgctttaa caggagagcg tactgcggtc agtgcagcga
//
