LOCUS       sequence-306167-       14346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS 
            mutant construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14346)
  AUTHORS   Lopacinski AB, Sweatt AJ, Smolko CM, Gray-Gaillard E, Borgman CA, 
            Shah M, Janes KA
  TITLE     Modeling the complete kinetics of coxsackievirus B3 reveals human 
            determinants of host-cell feedback.
  JOURNAL   Cell Syst. 2021 Apr 21;12(4):304-323.e13. doi: 
            10.1016/j.cels.2021.02.004. Epub 2021 Mar 18.
  PUBMED    33740397
REFERENCE   2  (bases 1 to 14346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2021.02.004"; journalName: "Cell Syst"; date: 
            "2021-04-21- 21"; volume: "12"; issue: "4"; pages: "304-323.e13"
COMMENT     Sequence Label: pSLIK 3xFLAG-MAVS-Ala148 hygro
FEATURES             Location/Qualifiers
     source          1..14346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2993..3058
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(4723..4747)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4797..6008
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5845..5864
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6133..6750,6751..6756,6757..6792,6793..6795,6796..6831,
                     6832..6834,6835..6870,6871..6879)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6917..7490
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7084..7101)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7311..7330
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7549..8568
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             7549..8568
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    9034..9622
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9087..9107)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9505..9516)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9625..9641)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9626..9642)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10151..10331
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10357..10374)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10363..10587
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10633..11061
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10720..10739)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10930..10951
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11070..11090)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11075..11404
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11255..11390
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11317..11336
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11452..11499
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11518..11892
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12022..12143
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12059..12078)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12113..12132
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12192..12208)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12192..12208)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12205..12227)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12216..12232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12240..12246,12247..12264,12265..12270))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12285..12306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12423..12440)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12594..13182)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12674..12693)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13353..14144,14145..14213))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13976..13995
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14214..14318)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactaga tggactacaa
     3001 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagac
     3061 tagtatgccg tttgctgaag acaagaccta taagtatatc tgccgcaatt tcagcaattt
     3121 ttgcaatgtg gatgttgtag agattctgcc ttacctgccc tgcctcacag caagagacca
     3181 ggatcgactg cgggccacct gcacactctc agggaaccgg gacaccctct ggcatctctt
     3241 caataccctt cagcggcggc ccggctgggt ggagtacttc attgcggcac tgaggggctg
     3301 tgagctagtt gatctcgcgg acgaagtggc ctctgtctac gagagctacc agcctcggac
     3361 ctcggaccgt cccccagacc cactggagcc accgtcactt cctgctgaga ggccagggcc
     3421 ccccacacct gctgcggccc acagcatccc ctacaacagc tgcagagaga aggagccaag
     3481 ttaccccatg cctgtccagg agaccgcggc gccagagtcc ccaggagaga attcagagca
     3541 agccctgcag acgctcagcc ccagagccat cccaaggaat ccagatggtg gccccctgga
     3601 gtcctcctct gacctggcag ccctcagccc tctgacctcc agcgggcatc aggagaagga
     3661 cacagaactg ggcagtaccc acacagcagg tgcgacctcc agcctcacac catcccgtgg
     3721 gcctgtgtct ccatctgtct ccttccagcc cctggcccgt tccaccccca gggcaagccg
     3781 cttgcctgga cccacagggt cagttgtatc tactggcacc tccttctcct cctcatcccc
     3841 tggcttggcc tctgcagggg ctgcagaggg taaacagggt gcagagagtg accaggccga
     3901 gcctatcatc tgctccagtg gggcagaggc acctgccaac tctctgccct ccaaagtgcc
     3961 taccaccttg atgcctgtga acacagtggc cctgaaagtg cctgccaacc cagcatctgt
     4021 cagcacagtg ccctccaagt tgccaactag ctcaaagccc cctggtgcag tgccttctaa
     4081 tgcgctcacc aatccagcac catccaaatt gcccatcaac tcaacccgtg ctggcatggt
     4141 gccatccaaa gtgcctacta gcatggtgct caccaaggtg tctgccagca cagtccccac
     4201 tgacgggagc agcagaaatg aggagacccc agcagctcca acacccgccg gcgccactgg
     4261 aggcagctca gcctggctag acagcagctt tgagaatagg ggccttgggt cggagctgag
     4321 taagcctggc gtgctggcat cccaggtaga cagcccgttc tcgggctgct tcgaggatct
     4381 tgccatcagt gccagcacct ccttgggcat ggggccctgc catggcccag aggagaatga
     4441 gtataagtcc gagggcacct ttgggatcca cgtggctgag aaccccagca tccagctcct
     4501 ggagggcaac cctgggccac ctgcggaccc ggatggcggc cccaggccac aagccgaccg
     4561 gaagttccag gagagggagg tgccatgcca caggccctca cctggggctc tgtggctcca
     4621 ggtggctgtg acaggggtgc tggtagtcac actcctggtg gtgctgtacc ggcggcgtct
     4681 gcactagcaa ttggaagact aatgcgtttt cgagatatct agacccagct ttcttgtaca
     4741 aagtggttga taacatctcg agccataaag atggttaatt aacccaccca agatctggcc
     4801 tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gcgctgccac
     4861 gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca ggacagcggc
     4921 ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca ttttaggacg
     4981 ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag gcgaggaaaa
     5041 gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt
     5101 atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat ttgggtcgcg
     5161 gttcttgttt gtggatcgct gtgatcgtca cttggtgagt agcgggctgc tgggctggcc
     5221 ggggctttcg tggccgccgg gccgctcggt gggacggaag cgtgtggaga gaccgccaag
     5281 ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg ggggttgggg ggagcgcagc
     5341 aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc
     5401 gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct
     5461 aatgcgggaa agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg
     5521 tgaagtttgt cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg
     5581 gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga
     5641 cgtcacccgt tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt
     5701 aggcttttct ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac
     5761 aggcgccgga cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt
     5821 tatgtaccta tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg
     5881 cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat
     5941 gtaattttca gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt
     6001 tgttagacga agcttggtac cgagctcgga tctccacccc gtaccggtcc tgcaggattt
     6061 aaatggcgcg ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
     6121 gtcgaattca ccatgtctag actggacaag agcaaagtca taaacggagc tctggaatta
     6181 ctcaatggtg tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt
     6241 gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca
     6301 atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa
     6361 gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg
     6421 gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag
     6481 ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc
     6541 gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag
     6601 gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg
     6661 ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc
     6721 ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac
     6781 ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac
     6841 gctcttgacg attttgacct tgacatgctc cccgggtaac taagtaagga tccgcggccg
     6901 cactagagga attccgcccc tctccccccc ccccccctaa cgttactggc cgaagccgct
     6961 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     7021 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     7081 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     7141 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     7201 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     7261 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     7321 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     7381 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     7441 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttaccggt
     7501 acgcgtgata gcttgccaca acccgtacca aagatggata gatccggaaa gcctgaactc
     7561 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     7621 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     7681 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     7741 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc
     7801 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     7861 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
     7921 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     7981 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     8041 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     8101 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     8161 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     8221 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     8281 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
     8341 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     8401 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     8461 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     8521 gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagag tagatgccga
     8581 ccgaacaaga gctgatttcg agaacgcctc agccagcaac tcgcgcgagc ctagcaaggc
     8641 aaatgcgaga gaacggcctt acgcttggtg gcacagttct cgtccacagt tcgctaagct
     8701 cgctcggctg ggtcgcggga gggccggtcg cagtgattca ggcccttctg gattgtgttg
     8761 gtccccaggg cacgattgtc atgcccacgc actcgggtga tctgactgat cccgcagatt
     8821 ggagatcgcc gcccgtgcct gccgattggg tgcagatctc tagatctgta caagtaaagc
     8881 ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     8941 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgttt
     9001 agtccctccc aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     9061 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     9121 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     9181 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     9241 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     9301 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     9361 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     9421 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     9481 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     9541 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     9601 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca
     9661 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     9721 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     9781 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     9841 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     9901 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     9961 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
    10021 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
    10081 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
    10141 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    10201 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    10261 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    10321 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
    10381 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
    10441 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
    10501 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
    10561 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
    10621 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
    10681 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
    10741 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
    10801 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
    10861 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
    10921 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
    10981 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
    11041 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
    11101 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
    11161 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    11221 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    11281 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    11341 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
    11401 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
    11461 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
    11521 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
    11581 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
    11641 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
    11701 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
    11761 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
    11821 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
    11881 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
    11941 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
    12001 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
    12061 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    12121 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
    12181 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    12241 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    12301 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    12361 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    12421 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    12481 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    12541 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    12601 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    12661 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    12721 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    12781 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    12841 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    12901 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    12961 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    13021 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    13081 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    13141 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    13201 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    13261 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    13321 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    13381 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    13441 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    13501 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    13561 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    13621 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    13681 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    13741 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    13801 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    13861 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    13921 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    13981 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    14041 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    14101 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    14161 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    14221 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    14281 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    14341 cctgac
//
