LOCUS       sequence-306172-        9013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for constitutive expression of 
            V5-tagged OAS1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9013)
  AUTHORS   Lopacinski AB, Sweatt AJ, Smolko CM, Gray-Gaillard E, Borgman CA, 
            Shah M, Janes KA
  TITLE     Modeling the complete kinetics of coxsackievirus B3 reveals human 
            determinants of host-cell feedback.
  JOURNAL   Cell Syst. 2021 Apr 21;12(4):304-323.e13. doi: 
            10.1016/j.cels.2021.02.004. Epub 2021 Mar 18.
  PUBMED    33740397
REFERENCE   2  (bases 1 to 9013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2021.02.004"; journalName: "Cell Syst"; date: 
            "2021-04-21- 21"; volume: "12"; issue: "4"; pages: "304-323.e13"
COMMENT     Sequence Label: pLX302 OAS1-V5 puro
FEATURES             Location/Qualifiers
     source          1..9013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1715..1739)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1741..1782
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1824..2412
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1877..1897)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2295..2306)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2484..2717
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2795..2916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2832..2851)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2886..2905
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2956..3091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3111..3130)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3112..3130)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3140..3157)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3140..3156)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3149..3171)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3298..3753
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3385..3404)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3595..3616
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3779..3883
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3884..3952,3953..4744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4102..4121)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4915..5503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5404..5423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5657..5674
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5791..5812
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5827..5832,5833..5850,5851..5857)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5865..5881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5870..5892
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5889..5905
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5889..5905
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5924..5944
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5926..5944
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5972..6198
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6199..6379
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6426..6551
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7044..7277
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7462..7506
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7681..8181
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8100..8118
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8203..8802
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8203..8222)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8699..8719
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8844..8961
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagttggcac catgatggat ctcagaaata ccccagccaa atctctggac
      661 aagttcattg aagactatct cttgccagac acgtgtttcc gcatgcaaat caaccatgcc
      721 attgacatca tctgtgggtt cctgaaggaa aggtgcttcc gaggtagctc ctaccctgtg
      781 tgtgtgtcca aggtggtaaa gggtggctcc tcaggcaagg gcaccaccct cagaggccga
      841 tctgacgctg acctggttgt cttcctcagt cctctcacca cttttcagga tcagttaaat
      901 cgccggggag agttcatcca ggaaattagg agacagctgg aagcctgtca aagcgagaga
      961 gcattttccg tgaagtttga ggtccaggct ccacgctggg gcaacccccg tgcgctcagc
     1021 ttcgtactga gttcgctcca gctcggggag ggggtggagt tcgatgtgct gcctgccttt
     1081 gatgccctgg gtcagttgac tggcagctat aaacctaacc cccaaatcta tgtcaagctc
     1141 atcgaggagt gcaccgacct gcagaaagag ggcgagttct ccacctgctt cacagaacta
     1201 cagagagact tcctgaagca gcgccccacc aagctcaaga gcctcatccg cctagtcaag
     1261 cactggtacc aaaattgtaa gaagaagctt gggaagctgc cacctcagta tgccctggag
     1321 ctcctgacgg tctatgcttg ggagcgaggg agcatgaaaa cacatttcaa cacagcccag
     1381 ggatttcgga cggtcttgga attagtcata aactaccagc aactctgcat ctactggaca
     1441 aagtattatg actttaaaaa ccccattatt gaaaagtacc tgagaaggca gctcacgaaa
     1501 cccaggcctg tgatcctgga cccggcggac cctacaggaa acttgggtgg tggagaccca
     1561 aagggttgga ggcagctggc acaagaggct gaggcctggc tgaattaccc atgctttaag
     1621 aattgggatg ggtccccagt gagctcctgg attctgctgg tgagacctcc tgcttcctcc
     1681 ctgccattca tccctgcccc tctccatgaa gctttgccaa ctttcttgta caaagtggtt
     1741 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct
     1801 aaccggtggc gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     1861 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     1921 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     1981 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     2041 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     2101 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     2161 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     2221 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     2281 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     2341 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     2401 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     2461 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     2521 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     2581 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     2641 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     2701 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     2761 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     2821 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2881 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     2941 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3001 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3061 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     3121 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     3181 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     3241 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     3301 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     3361 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     3421 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     3481 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     3541 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     3601 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     3661 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     3721 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     3781 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3841 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3901 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3961 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4021 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4081 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4141 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4201 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4261 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4321 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4381 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4441 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4501 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4561 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4621 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4681 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4741 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4801 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4861 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4921 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4981 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5041 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5101 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5161 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5221 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5281 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5341 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5401 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5461 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5521 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5581 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5641 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     5701 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     5761 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     5821 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     5881 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     5941 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     6001 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     6061 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     6121 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     6181 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6241 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6301 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6361 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     6421 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     6481 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     6541 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     6601 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     6661 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     6721 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     6781 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     6841 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     6901 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     6961 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     7021 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     7081 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     7141 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     7201 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     7261 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     7321 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     7381 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     7441 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     7501 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     7561 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     7621 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     7681 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     7741 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     7801 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     7861 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     7921 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     7981 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     8041 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     8101 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     8161 atcaccgacc tctctcccca gggggtacca ccggagctta ccatgaccga gtacaagccc
     8221 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     8281 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     8341 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     8401 gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     8461 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     8521 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     8581 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     8641 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     8701 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     8761 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagaattcta gatcttgaga
     8821 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     8881 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     8941 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc
     9001 cggctcgagg ggg
//
