LOCUS       sequence-30618-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Narimatsu M, Bose R, Pye M, Zhang L, Miller B, Ching P, Sakuma R, 
            Luga V, Roncari L, Attisano L, Wrana JL
  TITLE     Regulation of planar cell polarity by Smurf ubiquitin ligases.
  JOURNAL   Cell. 2009 Apr 17. 137(2):295-307.
  PUBMED    19379695
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Apr 17. 137(2):295-307."
COMMENT     Sequence Label: pGateway 3XFlag Prickle1 flag tagged
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..107
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    111..135
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctcgttt agtgaaccgt cagaattgat ctggtaccat ggactacaaa gaccatgacg
       61 gtgattataa agatcatgac atcgattaca aggatgacga tgacaagctt acaagtttgt
      121 acaaaaaagt tggcaccatg cctttggaga tggagcccaa gatgagcaaa ctggcctttg
      181 gctgtcagag aagttccaca tcagatgatg actctggctg tgcattggag gagtacgcct
      241 gggtcccccc gggcctgaga ccagagcaga tccagctcta ttttgcttgc ttaccagagg
      301 aaaaagttcc ttacgttaac agccccggag agaagcatcg gattaaacag cttttgtacc
      361 agttaccacc acatgataat gaggtacggt attgccagtc tttgagtgaa gaggagaaaa
      421 aagagttgca ggtgttcagt gctcagcgga agaaagaagc actgggaaga ggaacaatta
      481 agcttctgtc cagagcagtc atgcatgctg tgtgtgagca gtgtggtttg aagataaatg
      541 gaggtgaagt tgcagtgttc gcctcccgtg cgggccctgg tgtgtgctgg cacccatcct
      601 gttttgtctg tttcacgtgt aatgagctgc tggtcgacct catctatttt tatcaggatg
      661 gaaaaattca ctgtggcagg caccatgcag aactgctcaa accacggtgc tcagcatgtg
      721 acgagataat ttttgctgat gagtgcacag aagctg
//
