LOCUS       sequence-30619-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Narimatsu M, Bose R, Pye M, Zhang L, Miller B, Ching P, Sakuma R, 
            Luga V, Roncari L, Attisano L, Wrana JL
  TITLE     Regulation of planar cell polarity by Smurf ubiquitin ligases.
  JOURNAL   Cell. 2009 Apr 17. 137(2):295-307.
  PUBMED    19379695
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Apr 17. 137(2):295-307."
COMMENT     Sequence Label: pGateway 3XFlag Prickle2 flag tagged
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..90
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    94..118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgtcagaatt gatctggtac catggactac aaagaccatg acggtgatta taaagatcat
       61 gacatcgatt acaaggatga cgatgacaag cttacaagtt tgtacaaaaa agttggcacc
      121 atggtgacag tgatgccgct ggagatggag aagaccatca gcaaactcat gtttgacttt
      181 cagaggaact cgacctcaga tgatgactca ggctgtgctt tggaagagta tgcctgggtc
      241 ccgccgggtc tgaagcctga acaggtacac cagtactata gctgtctccc agaagagaaa
      301 gtcccttatg tcaacagtcc tggagagaaa ctgcgaatca agcagctact acaccagctg
      361 ccgccacatg acaatgaggt tcgatattgc aactccctgg atgaggaaga gaagagggag
      421 ctgaagcttt tcagcagcca gaggaaacgc gaaaacttgg gccgcgggaa tgtcaggcct
      481 ttcccagtca ccatgacagg agctatttgt gaacagtgcg gaggccagat caatggtgga
      541 gacatcgctg tgtttgcgtc acgcgctggc cacggcgttt gctggcaccc gccgtgcttc
      601 gtatgcactg tctgcaatga gctcctggtg gatctgatct acttttacca agatgggaag
      661 atatactgtg gcaggcacca tgctgagtgc ctgaagccgc gctgtgctgc ctgcgatgag
      721 atcatctttg cagatga
//
