LOCUS       sequence-306190-        1539 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  making transgenics expressing a UAS driven eGFP and NTR 2.0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Sharrock AV, Mulligan TS, Hall KR, Williams EM, White DT, Zhang L, 
            Emmerich K, Matthews F, Nimmagadda S, Washington S, Le KD, Meir-Levi
            D, Cox OL, Saxena MT, Calof AL, Lopez-Burks ME, Lander AD, Ding D, 
            Ji H, Ackerley DF, Mumm JS
  TITLE     NTR 2.0: a rationally engineered prodrug-converting enzyme with 
            substantially enhanced efficacy for targeted cell ablation.
  JOURNAL   Nat Methods. 2022 Feb;19(2):205-215. doi: 
            10.1038/s41592-021-01364-4. Epub 2022 Feb 7.
  PUBMED    35132245
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-021-01364-4"; journalName: "Nat Methods"; date: 
            "2022-02"; volume: "19"; issue: "2"; pages: "205-215"
COMMENT     Sequence Label: 5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
FEATURES             Location/Qualifiers
     source          1..1539
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(91..93,94..96,97..807)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(91..93,94..96,97..807)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(136..157)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(397..416)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     744..765
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             829..885
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 atgctgtgtt gtatcagaag aacaaagcct gtggagaaga atgaggaggc tgaccaggaa
       61 ctgcagcctg caggaagcac agctacaatg atggtgagca agggcgagga gctgttcacc
      121 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      181 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      241 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
      301 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      361 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      421 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      481 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
      541 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
      601 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
      661 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
      721 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      781 actctcggca tggacgagct gtacaagtac ttgtacaagg gaagcggagc tactaacttc
      841 agcctgctga agcaggctgg agacgtggag gagaaccccg ggcctatgac tattgttcaa
      901 gctgcccaat cccgctactc caccaaagcc tttgatgctt cgcgcaaatt gcctgaagag
      961 aaagtcgcgg cagtgaaaga gttaatccgc atgagtgcgt ccagtgtcaa ctcgcaacct
     1021 tggcatttta ttgtcgcgag cagtgaagag ggaaaagcgc gcatcgccaa agcaacacaa
     1081 ggtggttttg ctgctaatga gcgcaagatt ttggatgctt ctcacgttgt ggtgttctgc
     1141 gccaaaacgg cgattgatga agcgtaccta ctcgaccttt tggaaagcga agacaaagat
     1201 ggccgctacg ccgatgtcga agcaaaaaat ggcatgcacg ctggtcgttc atttttcgtc
     1261 aacatgcacc gctttgactt gaaagacgcg catcactgga tggaaaagca agtttacctc
     1321 aatgtgggga cgctgctatt gggtgcttct gcgatggaga tcgacgcggt gccaattgaa
     1381 ggcttcgatg ccaaggtgct tgatgaggag tttggtctgc gtgagaaggg ctttaccagc
     1441 gtggtgattg tgccgctggg ttaccatagc gaagacgatt ttaatgctaa gctgccaaaa
     1501 tcacgttggt cagcagagac tgtttttacc gaaatctaa
//
