LOCUS       sequence-306192-        1533 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  making transgenics expressing a UAS driven mCherry and NTR 2.0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Sharrock AV, Mulligan TS, Hall KR, Williams EM, White DT, Zhang L, 
            Emmerich K, Matthews F, Nimmagadda S, Washington S, Le KD, Meir-Levi
            D, Cox OL, Saxena MT, Calof AL, Lopez-Burks ME, Lander AD, Ding D, 
            Ji H, Ackerley DF, Mumm JS
  TITLE     NTR 2.0: a rationally engineered prodrug-converting enzyme with 
            substantially enhanced efficacy for targeted cell ablation.
  JOURNAL   Nat Methods. 2022 Feb;19(2):205-215. doi: 
            10.1038/s41592-021-01364-4. Epub 2022 Feb 7.
  PUBMED    35132245
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-021-01364-4"; journalName: "Nat Methods"; date: 
            "2022-02"; volume: "19"; issue: "2"; pages: "205-215"
COMMENT     Sequence Label: 5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:ta...
FEATURES             Location/Qualifiers
     source          1..1533
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..813
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(251..269)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     519..538
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     713..736
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             823..879
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 atgctgtgct gcatcagaag aactaaaccg gttgagaaga atgaagaggc cgatcaggag
       61 ctgcagtcga cggtaccgcg ggcccgggat ccaccggccg ccaccatggt gagcaagggc
      121 gaggaggaca acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      181 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      241 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
      301 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      361 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      421 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      481 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      541 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
      601 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgccgaggt caagaccacc
      661 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagctggac
      721 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgcgc cgagggccgc
      781 cactccaccg gcggcatgga cgagctgtac aagggaagcg gagctactaa cttcagcctg
      841 ctgaagcagg ctggagacgt ggaggagaac cccgggccta tgactattgt tcaagctgcc
      901 caatcccgct actccaccaa agcctttgat gcttcgcgca aattgcctga agagaaagtc
      961 gcggcagtga aagagttaat ccgcatgagt gcgtccagtg tcaactcgca accttggcat
     1021 tttattgtcg cgagcagtga agagggaaaa gcgcgcatcg ccaaagcaac acaaggtggt
     1081 tttgctgcta atgagcgcaa gattttggat gcttctcacg ttgtggtgtt ctgcgccaaa
     1141 acggcgattg atgaagcgta cctactcgac cttttggaaa gcgaagacaa agatggccgc
     1201 tacgccgatg tcgaagcaaa aaatggcatg cacgctggtc gttcattttt cgtcaacatg
     1261 caccgctttg acttgaaaga cgcgcatcac tggatggaaa agcaagttta cctcaatgtg
     1321 gggacgctgc tattgggtgc ttctgcgatg gagatcgacg cggtgccaat tgaaggcttc
     1381 gatgccaagg tgcttgatga ggagtttggt ctgcgtgaga agggctttac cagcgtggtg
     1441 attgtgccgc tgggttacca tagcgaagac gattttaatg ctaagctgcc aaaatcacgt
     1501 tggtcagcag agactgtttt taccgaaatc taa
//
