LOCUS       sequence-306194-        1536 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  making transgenics expressing a QUAS driven YFP and NTR 2.0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Sharrock AV, Mulligan TS, Hall KR, Williams EM, White DT, Zhang L, 
            Emmerich K, Matthews F, Nimmagadda S, Washington S, Le KD, Meir-Levi
            D, Cox OL, Saxena MT, Calof AL, Lopez-Burks ME, Lander AD, Ding D, 
            Ji H, Ackerley DF, Mumm JS
  TITLE     NTR 2.0: a rationally engineered prodrug-converting enzyme with 
            substantially enhanced efficacy for targeted cell ablation.
  JOURNAL   Nat Methods. 2022 Feb;19(2):205-215. doi: 
            10.1038/s41592-021-01364-4. Epub 2022 Feb 7.
  PUBMED    35132245
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-021-01364-4"; journalName: "Nat Methods"; date: 
            "2022-02"; volume: "19"; issue: "2"; pages: "205-215"
COMMENT     Sequence Label: 5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFP
FEATURES             Location/Qualifiers
     source          1..1536
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             88..804
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein"
                     /label=TagYFP
                     /note="mammalian codon optimized"
                     /translation="MVSKGEELFAGIVPVLIELDGDVHGHKFSVRGEGEGDADYGKLEI
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPKHMKMNDFFKSAMPEGYIQERTILFQDD
                     GKYKTRGEVKFEGDTLVNRIELKGKDFKEDGNILGHKLEYSFNSHNVYITPDKANNGLE
                     VNFKTRHNIEGGGVQLADHYQTNVPLGDGPVLIPINHYLSYQTDISKDRNEARDHMVLL
                     ESVSACSHTHGMDELYR"
     CDS             826..882
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 atgctgtgtt gtatcagaag aacaaagcct gtggagaaga atgaggaggc tgaccaggaa
       61 ctgcagcctg caggaagcac agctacaatg gttagcaaag gcgaggagct gttcgccggc
      121 atcgtgcccg tgctgatcga gctggacggc gacgtgcacg gccacaagtt cagcgtgcgc
      181 ggcgagggcg agggcgacgc cgactacggc aagctggaga tcaagttcat ctgcaccacc
      241 ggcaagctgc ccgtgccctg gcccaccctg gtgaccaccc tcacctacgg cgtacagtgc
      301 ttcgcccgct accccaagca catgaagatg aacgacttct tcaagagcgc catgcccgag
      361 ggctacatcc aggagcgcac catcctcttc caagacgacg gcaagtacaa gacccgcggc
      421 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg caaggacttc
      481 aaggaggacg gcaacatcct gggccacaag ctggagtaca gcttcaacag ccacaacgtc
      541 tacatcaccc ccgacaaggc caacaacggc ctggaggtga acttcaagac ccgccacaac
      601 atcgagggcg gcggcgtgca gctggccgac cactaccaga ccaacgtgcc cctgggcgac
      661 ggccccgtgc tgatccccat caaccactac ctgagctacc agaccgacat cagcaaggac
      721 cgcaacgagg cccgcgacca catggtgctc ctggagtccg tcagcgcctg cagccacacc
      781 cacggcatgg acgagctgta ccgctacttg tacaagggaa gcggagctac taacttcagc
      841 ctgctgaagc aggctggaga cgtggaggag aaccccgggc ctatgactat tgttcaagct
      901 gcccaatccc gctactccac caaagccttt gatgcttcgc gcaaattgcc tgaagagaaa
      961 gtcgcggcag tgaaagagtt aatccgcatg agtgcgtcca gtgtcaactc gcaaccttgg
     1021 cattttattg tcgcgagcag tgaagaggga aaagcgcgca tcgccaaagc aacacaaggt
     1081 ggttttgctg ctaatgagcg caagattttg gatgcttctc acgttgtggt gttctgcgcc
     1141 aaaacggcga ttgatgaagc gtacctactc gaccttttgg aaagcgaaga caaagatggc
     1201 cgctacgccg atgtcgaagc aaaaaatggc atgcacgctg gtcgttcatt tttcgtcaac
     1261 atgcaccgct ttgacttgaa agacgcgcat cactggatgg aaaagcaagt ttacctcaat
     1321 gtggggacgc tgctattggg tgcttctgcg atggagatcg acgcggtgcc aattgaaggc
     1381 ttcgatgcca aggtgcttga tgaggagttt ggtctgcgtg agaagggctt taccagcgtg
     1441 gtgattgtgc cgctgggtta ccatagcgaa gacgatttta atgctaagct gccaaaatca
     1501 cgttggtcag cagagactgt ttttaccgaa atctaa
//
