LOCUS       sequence-306202-        9676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Landing pad for Cre/lox-based recombinase-mediated cassette exchange
            (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted
            integration in CHO cells (site T9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Sergeeva D, Lee GM, Nielsen LK, Grav LM
  TITLE     Multi-copy targeted integration for accelerated development of 
            high-producing CHO cells.
  JOURNAL   ACS Synth Biol. 2020 Aug 24. doi: 10.1021/acssynbio.0c00322.
  PUBMED    32835482
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Aug 24. doi: 10.1021/acssynbio.0c00322."
COMMENT     Sequence Label: pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-site...
FEATURES             Location/Qualifiers
     source          1..9676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        222..432
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        244..440
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      291..426
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     353..372
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             489..1514
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             489..1514
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(1515..1548)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     polyA_signal    1557..1781
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        2553..2932
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2933..3136
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3087..3107
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3190..3199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3196..3888
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     polyA_signal    4033..4257
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4732..4749)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4732..4748)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4847..4864)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5018..5606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5098..5117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5728..6519,6520..6588))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6351..6370
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6589..6680)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6722..6741
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6747..6766
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    complement(7735..7768)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        7773..8935
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8004..8926
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8883..8903
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             8951..9652
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     polyA_signal    join(9662..9676,1..210)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
       61 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
      121 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
      181 ggcatgctgg ggatgcggtg ggctctatgg agtgcgatcc agcaggcaga agtatgcaaa
      241 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
      301 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
      361 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
      421 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
      481 agcacgtgat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
      541 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
      601 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
      661 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
      721 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
      781 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
      841 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
      901 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
      961 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     1021 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     1081 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     1141 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     1201 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     1261 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     1321 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     1381 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     1441 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     1501 gggcaaagga atagataact tcgtatagga tactttatac gaagttatac gtcgctctgt
     1561 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     1621 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     1681 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     1741 agacaatagc aggcatgctg gggatgcggt gggctctatg gaggtctgag ttggatatct
     1801 cactacactt tgatggccca atcttttttt aaaagcatat attcattgaa taaaatatga
     1861 gttcatattg gcatcctcat gcatgtctgg caagtacttg gctattattc acttcctcct
     1921 ctaaatccca tccattcttg ggtccataaa cctatcatct tagtttctcc tcatttcaaa
     1981 agaaattagg aaacagaagt ttgtaacagg aaatggattt atttatagtt ttgccactgg
     2041 ggagaaaaaa taactccatc ctgttctcta acatgctggt agtcttttac agtttattga
     2101 aaactatgag acgggatgga gaggtggttc agaggttaag agcaatggct gttcttccag
     2161 aggtcctgag ttcaattccc agcaaccaca tagtagctca cagctatctg taatgagatc
     2221 tggtgtgcaa gcatacatgc cggcagaaca ctgtatacaa aataaataaa tcttaaaaaa
     2281 aaaaagaaaa ctatgagaca aagacaagaa ggtatgtagc tttgctgtga gacagttcaa
     2341 catggctttt ctggggttgt gaggaggtca ctagtttcat atgacctgca gtatctcagt
     2401 tctcagcagt tcttgtttag catggagggg gtctctcttt acaattttgc catggttcca
     2461 gccctttctc aagcatggaa tcactgctat attagtgtcc tctttgttat ggggtaaaaa
     2521 cccagagacc attcaagcaa cacgtcgctg ttgacattga ttattgacta gttattaata
     2581 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     2641 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     2701 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     2761 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     2821 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     2881 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     2941 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     3001 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     3061 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     3121 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
     3181 agtgcgatcg ccaccatggc ccagtccaag cacggcctga ccaaggagat gaccatgaag
     3241 taccgcatgg agggctgcgt ggacggccac aagttcgtga tcaccggcga gggcatcggc
     3301 taccccttca agggcaagca ggccatcaac ctgtgcgtgg tggagggcgg ccccttgccc
     3361 ttcgccgagg acatcttgtc cgccgccttc atgtacggca accgcgtgtt caccgagtac
     3421 ccccaggaca tcgtcgacta cttcaagaac tcctgccccg ccggctacac ctgggaccgc
     3481 tccttcctgt tcgaggacgg cgccgtgtgc atctgcaacg ccgacatcac cgtgagcgtg
     3541 gaggagaact gcatgtacca cgagtccaag ttctacggcg tgaacttccc cgccgacggc
     3601 cccgtgatga agaagatgac cgacaactgg gagccctcct gcgagaagat catccccgtg
     3661 cccaagcagg gcatcttgaa gggcgacgtg agcatgtacc tgctgctgaa ggacggtggc
     3721 cgcttgcgct gccagttcga caccgtgtac aaggccaagt ccgtgccccg caagatgccc
     3781 gactggcact tcatccagca caagctgacc cgcgaggacc gcagcgacgc caagaaccag
     3841 aagtggcacc tgaccgagca cgccatcgcc tccggctccg ccttgcccaa gcttccgcgg
     3901 agccatggct tcccgccggc ggtggcggcg caggatgatg gcacgctgcc catgtcttgt
     3961 gcccaggaga gcgggatgga ccgtcaccct gcagcctgtg cttctgctag gatcaatgtg
     4021 tagacacagt ctctgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     4081 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     4141 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     4201 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggact
     4261 tgcgtagtga gtcgaataag ggcgacacaa aatttattct aaatgcataa taaatactga
     4321 taacatctta tagtttgtat tatattttgt attatcgttg acatgtataa ttttgatatc
     4381 aaaaactgat tttcccttta ttattttcga gatttatttt cttaattctc tttaacaaac
     4441 tagaaatatt gtatatacaa aaaatcataa ataatagatg aatagtttaa ttataggtgt
     4501 tcatcaatcg aaaaagcaac gtatcttatt taaagtgcgt tgcttttttc tcatttataa
     4561 ggttaaataa ttctcatata tcaagcaaag tgacaggcgc ccttaaatat tctgacaaat
     4621 gctctttccc taaactcccc ccataaaaaa acccgccgaa gcgggttttt acgttatttg
     4681 cggattaacg attactcgtt atcagaaccg cccagggggc ccgagcttaa gactggccgt
     4741 cgttttacaa cacagaaaga gtttgtagaa acgcaaaaag gccatccgtc aggggccttc
     4801 tgcttagttt gatgcctggc agttccctac tctcgccttc cgcttcctcg ctcactgact
     4861 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4921 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4981 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5041 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5101 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5161 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5221 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5281 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5341 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5401 atgtaggcgg tgctacagag ttcttgaagt ggtgggctaa ctacggctac actagaagaa
     5461 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5521 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5581 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5641 ctcagtggaa cgacgcgcgc gtaactcacg ttaagggatt ttggtcatga gcttgcgccg
     5701 tcccgtcaag tcagcgtaat gctctgctta ccaatgctta atcagtgagg cacctatctc
     5761 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     5821 gatacgggag ggcttaccat ctggccccag cgctgcgatg ataccgcgag aaccacgctc
     5881 accggctccg gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     5941 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6001 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatc gctacaggca tcgtggtgtc
     6061 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6121 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     6181 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcgctacata attctcttac
     6241 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     6301 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     6361 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6421 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6481 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6541 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatat tcttcctttt
     6601 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6661 tatttagaaa aataaacaaa taggggtcag tgttacaacc aattaaccaa ttctgaacat
     6721 tatcgcgagc ccatttatac ctgaatatgg ctcataacac cccttgtttg cctggcggca
     6781 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
     6841 atggtagtgt ggggactccc catgcgagag tagggaactg ccaggcatca aataaaacga
     6901 aaggctcagt cgaaagactg ggcctttcgc ccgggctaat tatggggtgt cgcccttatt
     6961 cgactcagtc ggtgtgataa actattcttc atttgccagg ggtgaacgaa actccagtgc
     7021 acatatctca gctctatagc tgagagatac ctcataaaac ataggatact taggtaaatt
     7081 tgagtttcag gtgaagattt tttaagtata atcatgtctt gcacaatgtt tgggattcat
     7141 ttttattgat taaatctgac agtccttttt ttttctttcc tttaaaaaac tttttttttt
     7201 ttttaaagac agggtcttat gtatcctaag gtgaccttga acttgccttg tagatgagtc
     7261 tggctttgag cccctaatac ctacctccac ctctcaaatc ctaggattac aggtgtatac
     7321 cactctaccc atttgacaat tctacttttg agccatctta aaggtaaaaa gtacacttat
     7381 ttatcatatg cctgtgtgtg gaagtcagaa caacttgtgg tagttggttc tttccttcat
     7441 gggggccctg ggggttagac tccaattgtc aggcttgctg gtaaatgcct tattaccacc
     7501 ttgatgtgga ggtacaaata atgattcaca ggactaggtt caaatattaa atataattta
     7561 ttccggggag aagtcactta cagaagaagc agacagctgc tgcaggttcc agctctaagg
     7621 atccgcacag gctactccct gtggcccaac cagacggaag tgagagccac ctgctgggac
     7681 cccagaccta aactcactcc cagtcaggtc ctcacctgta accacaagca gcgtataact
     7741 tcgtatagca tacattatac gaagttatgt gaggctccgg tgcccgtcag tgggcagagc
     7801 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
     7861 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     7921 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     7981 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
     8041 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctccag tacgtgattc
     8101 ttgatcccga gctggagcca ggggcgggcc ttgcgcttta ggagcccctt cgcctcgtgc
     8161 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
     8221 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
     8281 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caggatctgc acactggtat
     8341 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
     8401 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
     8461 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     8521 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctccagg
     8581 gggctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     8641 gaaaggggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     8701 gtccaggcac ctcgattagt tctggagctt ttggagtacg tcgtctttag gttgggggga
     8761 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     8821 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     8881 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagacg
     8941 tcattcggtc atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg
     9001 caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
     9061 cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat
     9121 cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc
     9181 cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga
     9241 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
     9301 caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac
     9361 actcggctgg gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag
     9421 aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac
     9481 atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta
     9541 cagactggaa agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc
     9601 agtggccaga tactgcgacc tccctagcaa actggggcac aagcttaatt aaaacgtgga
     9661 tctgtgcctt ctagtt
//
