LOCUS       sequence-306204-        9589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Landing pad for Cre/lox-based recombinase-mediated cassette exchange
            (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted
            integration in CHO cells (site A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Sergeeva D, Lee GM, Nielsen LK, Grav LM
  TITLE     Multi-copy targeted integration for accelerated development of 
            high-producing CHO cells.
  JOURNAL   ACS Synth Biol. 2020 Aug 24. doi: 10.1021/acssynbio.0c00322.
  PUBMED    32835482
REFERENCE   2  (bases 1 to 9589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Aug 24. doi: 10.1021/acssynbio.0c00322."
COMMENT     Sequence Label: pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-sit...
FEATURES             Location/Qualifiers
     source          1..9589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(597..614)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(597..613)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(712..729)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(883..1471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(963..982)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1593..2384,2385..2453))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2216..2235
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2454..2545)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2587..2606
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2612..2631
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    complement(3600..3633)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3638..4800
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3869..4791
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4748..4768
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4811..4820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4817..5527
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4962..4980)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5230..5249
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5537..5761
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5774..5794)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5779..6108
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5959..6094
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6021..6040
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6175..6969
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6229..6248)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6839..6858
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(6970..7003)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     polyA_signal    7012..7236
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        8007..8386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8387..8590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8541..8561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      8644..8653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8650..9342
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     polyA_signal    join(9487..9589,1..122)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
       61 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
      121 ggacttgcgt agtgagtcga ataagggcga cacaaaattt attctaaatg cataataaat
      181 actgataaca tcttatagtt tgtattatat tttgtattat cgttgacatg tataattttg
      241 atatcaaaaa ctgattttcc ctttattatt ttcgagattt attttcttaa ttctctttaa
      301 caaactagaa atattgtata tacaaaaaat cataaataat agatgaatag tttaattata
      361 ggtgttcatc aatcgaaaaa gcaacgtatc ttatttaaag tgcgttgctt ttttctcatt
      421 tataaggtta aataattctc atatatcaag caaagtgaca ggcgccctta aatattctga
      481 caaatgctct ttccctaaac tccccccata aaaaaacccg ccgaagcggg tttttacgtt
      541 atttgcggat taacgattac tcgttatcag aaccgcccag ggggcccgag cttaagactg
      601 gccgtcgttt tacaacacag aaagagtttg tagaaacgca aaaaggccat ccgtcagggg
      661 ccttctgctt agtttgatgc ctggcagttc cctactctcg ccttccgctt cctcgctcac
      721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      781 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg gctaactacg gctacactag
     1321 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1441 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1501 tgacgctcag tggaacgacg cgcgcgtaac tcacgttaag ggattttggt catgagcttg
     1561 cgccgtcccg tcaagtcagc gtaatgctct gcttaccaat gcttaatcag tgaggcacct
     1621 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1681 actacgatac gggagggctt accatctggc cccagcgctg cgatgatacc gcgagaacca
     1741 cgctcaccgg ctccggattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1801 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1861 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccatcgctac aggcatcgtg
     1921 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1981 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2041 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcgct acataattct
     2101 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2161 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     2221 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2281 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2341 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2401 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catattcttc
     2461 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2521 gaatgtattt agaaaaataa acaaataggg gtcagtgtta caaccaatta accaattctg
     2581 aacattatcg cgagcccatt tatacctgaa tatggctcat aacacccctt gtttgcctgg
     2641 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
     2701 cgccgatggt agtgtgggga ctccccatgc gagagtaggg aactgccagg catcaaataa
     2761 aacgaaaggc tcagtcgaaa gactgggcct ttcgcccggg ctaattatgg ggtgtcgccc
     2821 ttattcgact cagtcggtgt gaagcttacc cccaggattt cctgatacta ctttagaaaa
     2881 aaactagctt taatatattg gcatcaatat attatttttc ctctctgcct ccttttcttt
     2941 ctttttttaa ttttttatta atttattttt tacagtccaa tcccagttcc ccctccctcc
     3001 tgtcctcctg ttcctcctac tccccctgca tctgctcctt ggagagggta aggcctcccc
     3061 ttttcctttc ttgaggggaa aatttttaag gcagctaaga tccttctcct gaaggcactc
     3121 actgttagtg atctaataca ataagggctg tggtgaaaac aagtacaaga agattaattc
     3181 tctggcacag aagagaaaga gccagatgac tttcaaggga tgtttgagtt gactcttgaa
     3241 agcttgtgac agagctcaga gaggtgttca aaacagagcc agccagggtc agagtccctc
     3301 aggtcattca gagttggaga ctcggcaaga gtaggcctgc agacattcag cctctgctac
     3361 agacagatgt gacttagggc cttttctaag agaggaattg cagtgttatt acccagctag
     3421 tggacagcta atgtgttcct cctgaagaag actatgaaac aagtattctg tagagtgagg
     3481 gcaagatgga agaccagcat gggggtggga gggggtacat tatacctttg tggttctcca
     3541 cataaggtag gttgctttca tttaagagat cacttgcttt gggctgtttt aagcagcgta
     3601 taacttcgta tagcatacat tatacgaagt tatgtgaggc tccggtgccc gtcagtgggc
     3661 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     3721 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     3781 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     3841 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     3901 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tccagtacgt
     3961 gattcttgat cccgagctgg agccaggggc gggccttgcg ctttaggagc cccttcgcct
     4021 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac
     4081 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct
     4141 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccagga tctgcacact
     4201 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt
     4261 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct
     4321 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca
     4381 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct
     4441 ccagggggct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca
     4501 caaaggaaag gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg
     4561 gcgccgtcca ggcacctcga ttagttctgg agcttttgga gtacgtcgtc tttaggttgg
     4621 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg
     4681 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg
     4741 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
     4801 agacgtcatc gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     4861 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     4921 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     4981 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     5041 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     5101 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     5161 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     5221 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     5281 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     5341 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     5401 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     5461 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     5521 caagtagaca cagtctctgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     5581 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     5641 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     5701 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     5761 gagtgcgatc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5821 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     5881 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5941 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6001 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     6061 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     6121 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     6181 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     6241 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     6301 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     6361 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     6421 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     6481 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     6541 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     6601 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     6661 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     6721 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     6781 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     6841 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     6901 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     6961 ttcttctgaa taacttcgta taggatactt tatacgaagt tatacgtcgc tctgtgcctt
     7021 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     7081 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     7141 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     7201 atagcaggca tgctggggat gcggtgggct ctatggaggt ctgagtatgc ctccattgat
     7261 tctttcagtg gcaccagaca ttgttccatg ttccgtgtgt tgtgcttgct ttattgctgt
     7321 tttctcttat gatttacctt aatggtataa gcaacttcct tacaggttgc tacaattaaa
     7381 acaatcacat agctaccctt tgcatggtaa gctgggaaag cacacataca acattagtag
     7441 aggcagtcca taaaaagcaa gctaaggata cttttgctat ataatctccc ttttttttgt
     7501 ttgtttgttt gtttttcaag ttagggtttc tctgtggctt tggaggctgt cttggaacta
     7561 gctcttgtag accaggctgg tcttgaactc acagagatct ccctgcctgt gcctcccgag
     7621 tgctgggact aaaggcatgc gccaccactg cccagtttat ataatccctt ttttttttca
     7681 ctcatctagt ttatttctat gtttatttct cttttatctt ttaatccaat aatttgtctt
     7741 caaagaggcc aatattttta aagattataa ttctgcattg ttatatttga catatatatt
     7801 aaaattttat gaaaaataag cttcatcttc agttttcaat tggtgtttgt atagaataac
     7861 catctcagac gttagctgtg gtcctggcta gacttatgtc aacctgccag aagccagagt
     7921 cagggaagag gaactcccaa ttgagaaaat gactcaataa gatcaagctg tagtcaagtc
     7981 tataaggtat tttctacgtc gctgttgaca ttgattattg actagttatt aatagtaatc
     8041 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     8101 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     8161 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     8221 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     8281 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     8341 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     8401 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     8461 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     8521 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     8581 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaatagtgcg
     8641 atcgccacca tggcccagtc caagcacggc ctgaccaagg agatgaccat gaagtaccgc
     8701 atggagggct gcgtggacgg ccacaagttc gtgatcaccg gcgagggcat cggctacccc
     8761 ttcaagggca agcaggccat caacctgtgc gtggtggagg gcggcccctt gcccttcgcc
     8821 gaggacatct tgtccgccgc cttcatgtac ggcaaccgcg tgttcaccga gtacccccag
     8881 gacatcgtcg actacttcaa gaactcctgc cccgccggct acacctggga ccgctccttc
     8941 ctgttcgagg acggcgccgt gtgcatctgc aacgccgaca tcaccgtgag cgtggaggag
     9001 aactgcatgt accacgagtc caagttctac ggcgtgaact tccccgccga cggccccgtg
     9061 atgaagaaga tgaccgacaa ctgggagccc tcctgcgaga agatcatccc cgtgcccaag
     9121 cagggcatct tgaagggcga cgtgagcatg tacctgctgc tgaaggacgg tggccgcttg
     9181 cgctgccagt tcgacaccgt gtacaaggcc aagtccgtgc cccgcaagat gcccgactgg
     9241 cacttcatcc agcacaagct gacccgcgag gaccgcagcg acgccaagaa ccagaagtgg
     9301 cacctgaccg agcacgccat cgcctccggc tccgccttgc ccaagcttcc gcggagccat
     9361 ggcttcccgc cggcggtggc ggcgcaggat gatggcacgc tgcccatgtc ttgtgcccag
     9421 gagagcggga tggaccgtca ccctgcagcc tgtgcttctg ctaggatcaa tgtgtagaca
     9481 cagtctctgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     9541 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgagga
//
