LOCUS       sequence-306222-       11055 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11055)
  AUTHORS   Mann SE, Zhou Z, Landry LG, Anderson AM, Alkanani AK, Fischer J, 
            Peakman M, Mallone R, Campbell K, Michels AW, Nakayama M
  TITLE     Multiplex T Cell Stimulation Assay Utilizing a T Cell Activation 
            Reporter-Based Detection System.
  JOURNAL   Front Immunol. 2020 Apr 9;11:633. doi: 10.3389/fimmu.2020.00633. 
            eCollection 2020.
  PUBMED    32328071
REFERENCE   2  (bases 1 to 11055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Immunol."; date: "2020-04-9"; volume: "11:633. doi"; pages: " 
            10.3389/fimmu.2020.00633. eCollection 2020"
COMMENT     Sequence Label: A2_uSFFV
FEATURES             Location/Qualifiers
     source          1..11055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(36..53)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(207..795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(287..306)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(966..1757,1758..1826))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1589..1608
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1827..1931)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1999..2017
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2055..2077)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2177..2196
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2410..2429
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2719..2737
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2719..2736
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             2762..2995
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     3085..3101
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3086..3102
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3105..3693
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3211..3222
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3620..3640
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4094..4150)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      5251..5260
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5296..5703)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    6445..6562
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6847..6891)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7076..7309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7806..7931
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7978..8611
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8779..8913
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8803..8822)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8857..8876
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9338..9358)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          9488..9553
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(10687..11016)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10701..10836
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11001..11021
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
       61 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      121 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      181 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      241 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      301 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      361 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      421 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      481 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      541 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      601 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      661 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      721 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      781 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      841 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
      901 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
      961 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1021 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1081 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     1141 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1201 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1261 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1321 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1381 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1441 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1501 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1561 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     1621 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     1681 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     1741 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     1801 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     1861 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     1921 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     1981 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     2041 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     2101 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     2161 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca ttcgacgctc
     2221 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     2281 cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
     2341 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     2401 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     2461 gccggccacg atgcgtccgg cgtagaggat ctggctagcg atgaccctgc tgattggttc
     2521 gctgaccatt tccgggtgcg ggacggcgtt accagaaact cagaaggttc gtccaaccaa
     2581 accgactctg acggcagttt acgagagaga tgatagggtc tgcttcagta agccagatgc
     2641 tacacaatta ggcttgtaca tattgtcgtt agaacgcggc tacaattaat acataacctt
     2701 atgtatcata cacatacgat ttaggtgaca ctatagaata cacggaatta attctagatg
     2761 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
     2821 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
     2881 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
     2941 ccagtacaag caaaaagcag atcttgtctt cgttgggagt gaattagccc ttccagtccc
     3001 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
     3061 ggtaccgagc tcgaattaat tccctcgagg tcgacggtat cgatgcgggg aggcggccca
     3121 aagggagatc cgactcgtct gagggcgaag gcgaagacgc ggaagaggcc gcagagccgg
     3181 cagcaggccg cgggaaggaa ggtccgctgg attgagggcc gaagggacgt agcagaagga
     3241 cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc caaggaaagg acgatgattt
     3301 ccccgacaac accacggaat tgtcagtgcc caacagccga gcccctgtcc agcagcgggc
     3361 aaggcaggcg gcgatgagtt ccgccgtggc aatagggagg gggaaagcga aagtcccgga
     3421 aaggagctga caggtggtgg caatgcccca accagtgggg gttgcgtcag caaacacagt
     3481 gcacaccacg ccacgttgcc tgacaacggg ccacaactcc tcataaagag acagcaacca
     3541 ggatttatac aaggaggaga aaatgaaagc catacgggaa gcaatagcat gatacaaagg
     3601 cattaaagca gcgtatccac atagcgtaaa aggagcaaca tagttaagaa taccagtcaa
     3661 tctttcacaa attttgtaat ccagaggttg attatcgata agcttgatat caagcttgca
     3721 tgcctgcagg tcgttacatg tctcgatccc acttaactat cttgggctgt gacaaagtca
     3781 catggttcac acggcaggca tactcatctt tttcagtggg ggtgaattca gtgtagtaca
     3841 agagatagaa agaccagtcc ttgctgaaag acaagtctga atgctccact ttttcaattc
     3901 tctctccatt cttcagtaag tcaacttcaa tgtcggatgg atgaaaccca gacacatagc
     3961 aattcaggaa atttgacttt ccattctctg ctggatgacg tgagtaaacc tgaatctttg
     4021 gagtacgctg gatagcctcc aggccagaaa gagagagtag cgcgagcaca gctaaggcca
     4081 cggagcgaga catgggaccg gggttttctt ccacgtctcc tgcttgcttt aacagagaga
     4141 agttcgtggc tccggagccc actttacaag ctgtgagaga cacatcagag ccctgggcac
     4201 tgtcactgct tgcagcctga gagtagctcc ctccttttct atctgagctc ttcctcctcc
     4261 acatcacagc agcgaccaca gctccagtga tcacagctcc aaagagaacc aggccagcaa
     4321 tgatgcccac gatggggatg gtgggctggg aagacggctc ccatctcagg gtgaggggct
     4381 tgggcaaacc ctcatgctgc acatggcagg tgtatctctg ctcctgtcca gaaggcacca
     4441 ccacagccgc ccacttctgg aaggttccat cccctgcagg cctggtctcc acgagctccg
     4501 tgtcctgggt ctggtcctcc ccatcccgct gccaggtcag tgtgatctcc gcagggtaga
     4561 agctcagggc ccagcacctc agggtggctt catggtcaga gacagcatgg tgagtcatat
     4621 gcgttttggg ggcgtccgtg cgctgcagcg tctccttccc gttctccagg tatctgcgga
     4681 gccactccac gcacgtgccc tccaggtagg ctctcaactg ctccgccaca tgggccgcct
     4741 cccacttgtg cttggtggtc tgagctgcca tgtccgccgc ggtccaagag cgcaggtcct
     4801 ctttcagggc gatgtaatcc ttgccgtcgt aggcgtactg gtggtacccg cggaggaagc
     4861 gccagtccga ccccacgtcg cagccataca tcctctggac ggtgtgagaa ccggcctcgc
     4921 tctggttgta gtagccgcgc agggtcccca ggtccactcg gtgagtctgt gagtgggcct
     4981 tcactttccg tgtctccccg tcccaatact ccggaccctc ctgctctatc cacggcgccc
     5041 gcggctccat cctctggctc gcggcgtcgc tgtcgaaccg cacgaactgc gtgtcgtcca
     5101 cgtagcccac tgcgatgaag cggggctccc cgcggccggg ccgggacacg gatgtgaaga
     5161 aatacctcat ggagtgagag cccgcccagg tctgggtcag ggccagagcc cccgagagta
     5221 gcaggacgag ggttcggggc gccatgacgg ccatggtggc agacgcgtga gaattctcga
     5281 gagctccaga tcccccccgg gcgactcagt ctgtcggagg actggcgcgc cgagtgaggg
     5341 gttgtgagct cttttataga gctcgggaag cagaagcgcg cgaacagaag cgagaagcag
     5401 gctgattggt taattcaaat aaggcgcagg gtcatttcag gtccttgggg gagcctggaa
     5461 acatctgatg ggtcttaaga aactgctgag ggttgggcca tatctgggga ccatctgttc
     5521 ttggcctcgg gccggggccg aaactgcggt gaccatctgt tcttggcccc gggccggggc
     5581 cgaaactgct caccgcagat atcctgtttg gcccaacgtt agctattttc atgtacccgc
     5641 ccttgatctg aacttctcta ttcttggttt ggtatttttc catgccttgc aaaatggcgt
     5701 tactgcagct agcttgccaa acctacaggt ggggtctttc attcccccct ttttctggag
     5761 actaaataaa atcttttatt ttatcggggc tgcaggactc cgcgcctaca gctcaagcca
     5821 catccgaagg gggagggagc cgggagctgc gcgcggggcc gcgcgccgcg cccgctggga
     5881 attggggccc tagggggagg gcggaggcgc cgacgaccgc ggcacttacc gttcgcggcg
     5941 tggcgcccgg tggtccccaa ggggagggaa gggggaggcg gggcgaggac agtgaccgga
     6001 gtctcctcag cggtggcttt tctgcttggc agcctcagcg gctggcgcca aaaccggact
     6061 ccgcccactt cctcgcccct gcggtgcgag ggtgtggaat cctccagacg ctgggggagg
     6121 gggagttggg agcttaaaaa ctagtacccc tttgggacca ctttcagcag cgaactctcc
     6181 tgtacaccag gggtcagttc cacagacgcg ggccaggggt gggtcattgc ggcgtgaaca
     6241 ataatttgac tagaagttga ttcgggtgtt tccggaaggg gccgagtcaa tccgccgagt
     6301 tggggcacgg aaaacaaaaa gggaaggcta ctaagatttt tctggcgggg gttatcattg
     6361 gcgtaactgc agggaccacc tcccgaattc gatatcaagc ttatcgatcc aaactggatc
     6421 tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc
     6481 tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc
     6541 aatcccccct tttcttttaa aattgtggat gaatactgcc atttggcgat accgtcgaga
     6601 tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc
     6661 ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata
     6721 tccctgccta actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa
     6781 gcctcctact atcattatga ataattttat ataccacagc caatttgtta tgttaaacca
     6841 attccacaaa cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg
     6901 gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc
     6961 ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc
     7021 attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag
     7081 ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc
     7141 ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg
     7201 ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc
     7261 gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc
     7321 tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc
     7381 aatttttact actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc
     7441 tccaggtctg aagatcagcg gccggccgct tgctgtgcgg tggtcttact tttgttttgc
     7501 tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca
     7561 caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg
     7621 gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagga
     7681 ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt
     7741 ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta
     7801 atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc
     7861 gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg
     7921 cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc
     7981 tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca
     8041 acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg
     8101 ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag
     8161 tacaggcaaa aagcagctgc ttatatgcag gatctgaggg ctcgccactc cccagtcccg
     8221 cccaggccac gcctccctgg aaagtcccca gcggaaagtc ccttgtagca agctcgatat
     8281 cagcagttct tgaagtactc cggatgcagc tctcgggcca cgtgatgaaa tgctaggcgg
     8341 ctgtcaaacc tccactctaa cacttctctc tccgggtcat ccatcccatg caggctcaca
     8401 gggtgtaaca agctggtgtt ctctccttta ttggcctctt ctaccttatc tggctcaact
     8461 ggtactagct tgtagcacca tccaaaggtc agtggatatc tgacccctgg ccctggtgtg
     8521 tagttctgct aatcagggaa gtagccttgt gtgtggtaga tccacagatc aaggatatct
     8581 tgtcttcttt gggagtgaat tagcccttcc aactactaag tttgtagtac atatttaaca
     8641 aatacaattt ctttaaaatg aaaataattc agaggaatca caggtttaga gtaaatgaaa
     8701 ccacaggtaa ttggcagtgg taatagggta tggggtggga agtttgggat gattttggtt
     8761 agcttgagtt atccagttga tccagacatg ataagataca ttgatgagtt tggacaaacc
     8821 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     8881 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     8941 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     9001 ggtatggctg attatgatct ctagtcaagg cactatacat caaatattcc ttattaaccc
     9061 ctttacaaat taaaaagcta aaggtacaca atttttgagc atagttatta atagcagaca
     9121 ctctatgcct gtgtggagta agaaaaaaca gtatgttatg attataactg ttatgcctac
     9181 ttataaaggt tacagaatat ttttccataa ttttcttgta tagcagtgca gctttttcct
     9241 ttgtggtgta aatagcaaag caagcaagag ttctattact aaacacagca tgactcaaaa
     9301 aacttagcaa ttctgaagga aagtccttgg ggtcttctac ctttctcttc ttttttggag
     9361 gagtagaatg ttgagagtca gcagtagcct catcatcact agatggcatt tcttctgagc
     9421 aaaacaggtt ttcctcatta aaggcattcc accactgctc ccattcatca gttccatagg
     9481 ttggaatcta aaatacacaa acaattagaa tcagtagttt aacacattat acacttaaaa
     9541 attttatatt taccttagag ctttaaatct ctgtaggtag tttgtccaat tatgtcacac
     9601 cacagaagta aggttccttc acaaagatcc ggggcccact cataaatcca gttgccgcca
     9661 cggtagccaa tcaccgtatc gtataaatca tcgtcggtac gttcggcatc gctcatcaca
     9721 atacgtgcct ggacgtcgag gatttcgcgt gggtcaatgc cgcgccagat ccacatcaga
     9781 cggttaatca tgcgatacca gtgagggatg gttttaccat caagggccga ctgcacaggc
     9841 ggttgtgcgc cgtgattaaa gcggcggact agcgtcgagg tttcaggatg tttaaagcgg
     9901 ggtttgaaca gggtttcgct caggtttgcc tgtgtcatgg atgcagcctc cagaatactt
     9961 actggaaact attgtaaccc gcctgaagtt aaaaagaaca acgcccggca gtgccaggcg
    10021 ttgaaaagat tagcgaccgg agattggcgg gacgaatacg acgcccatat cccacggctg
    10081 ttcaatccag gtatcttgcg ggatatcaac aacatagtca tcaaccagcg gacgaccagc
    10141 cggttttgcg aagatggtga caaagtgcgc ttttggatac atttcacgaa tcgcaaccgc
    10201 agtaccaccg gtatccacca ggtcatcaat aacgatgaag ccttcgccat cgccttctgc
    10261 gcgtttcagc actttaagct cgcgctggtt gtcgtgatcg tagctggaaa tacaaacggt
    10321 atcgacatga cgaataccca gttcacgcgc cagtaacgca cccggtacag accgccacgg
    10381 cttacggcaa taatgccttt ccattgttca gaaggcatca gtcggcttgc gagtttacgt
    10441 gcatggatct gcaacatgtc ccaggtgacg atgtattttt cgctcatgtg aagtgtccca
    10501 gcctgtttat ctacggctta aaaagtgttc gaggggaaaa taggttgcgc gagattatag
    10561 agatctggcg cactaaaaac cagtatttca catgagtccg cgtcttttta cgcactgcct
    10621 ctccctgacg cgggataaag tggtattctc aaacatatct cgcaagcctg tcttgtgtcc
    10681 aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
    10741 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
    10801 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
    10861 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
    10921 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
    10981 ggagcctggg gactttccac accctaactg acacacattc cacagctgca ttaatgaatc
    11041 ggccaacgcg cgggg
//
