LOCUS       sequence-306225-        6161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments 
            of mouse Cd36 enhancers at Chr5:17918617_17920400.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Kuang Z, Wang Y, Li Y, Ye C, Ruhn KA, Behrendt CL, Olson EN, Hooper 
            LV
  TITLE     The intestinal microbiota programs diurnal rhythms in host 
            metabolism through histone deacetylase 3.
  JOURNAL   Science. 2019 Sep 27;365(6460):1428-1434. doi: 
            10.1126/science.aaw3134.
  PUBMED    31604271
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2019-09-27- 27"; volume: "365"; 
            issue: "6460"; pages: "1428-1434"
COMMENT     Sequence Label: pGL4.24-Cd36-enhancer7
FEATURES             Location/Qualifiers
     source          1..6161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1008..1129
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1045..1064)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1099..1118
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(1181..1300)
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             complement(1304..2953)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      2950..2959
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2986..3017)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    4844..4935
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(4876..4895)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    4949..4997
                     /note="synthetic polyadenylation signal"
     CDS             join(5102..5170,5171..5962)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgctgccg
      781 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
      841 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac
      901 ggatccttat cgattttacc acatttgtag aggttttact tgctttaaaa aacctcccac
      961 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     1021 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1081 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgct
     1141 cgaagcggcc ggccgccccg actctagagt cgcggcctta gacgttgatc ctggcgctgg
     1201 cgcaagcagc agggtgtcta tccatgccgc tctcctgggc gcagctcatg ggcagggtgc
     1261 cggcggcctg ctcctccacc tcgggaggga agccgtgaga attcacggcg atcttgccgc
     1321 ccttcttggc cttaatgaga atctcgcgga tcttgcgggc gtccaacttg ccggtcagtc
     1381 ctttaggcac ctcgtccacg aacacaacac caccgcgcag cttcttggcg gttgtaacct
     1441 ggctggccac atagtccacg atctccttct cggtcatggt tttaccgtgt tccagcacga
     1501 cgactgcggc gggcagctcg ccggcatcgt cgtcgggcag gccggcgacc ccggcgtcga
     1561 agatgttggg gtgttgcagc aggatgctct ccagttcggc tggggctacc tggtagccct
     1621 tgtatttgat caggctcttc agccggtcca cgatgaagaa gtgctcgtcc tcgtcccagt
     1681 aggcgatgtc gccgctgtgc agccagccgt ccttgtcgat gagagcgttt gtagcctcgg
     1741 ggttgttaac gtagccgctc atgatcatgg ggccacggac gcacagctcg ccgcgctggt
     1801 tcacacccag tgtcttaccg gtgtccaagt ccaccacctt agcctcgaag aagggcacca
     1861 ccttgcctac tgcgccaggc ttgtcgtccc cttcgggggt gatcagaatg gcgctggttg
     1921 tttctgtcag gccgtagccc tggcggatgc ctggtaggtg gaagcgtttg gccacggcct
     1981 cacctacctc cttgctgagc ggcgccccgc cgctggcgat ctcgtgcaag ttgcttaggt
     2041 cgtacttgtc gatgagagtg ctcttagcga agaagctaaa tagtgtgggc accagcaggg
     2101 cagattgaat cttatagtct tgcaagctgc gcaagaatag ctcctcctcg aagcggtaca
     2161 tgagcacgac ccgaaagccg cagatcaagt agcccagcgt ggtgaacatg ccgaagccgt
     2221 ggtgaaatgg caccacgctg aggatagcgg tgtcggggat gatctggttg ccgaagatgg
     2281 ggtcgcgggc atgactgaat cggacacaag cggtgcggtg cggtagggct acgcccttgg
     2341 gcaatccggt actgccacta ctgttcatga tcagggcgat ggttttgtcc cggtcgaagc
     2401 tctcgggcac gaagtcgtac tcgttgaagc cgggtggcaa atgggaagtc acgaaggtgt
     2461 acatgctttg gaagccctgg tagtcggtct tgctatccat gatgatgatc ttttgtatga
     2521 tcggtagctt cttttgcacg ttgaggatct tttgcagccc tttcttgctc acgaatacga
     2581 cggtgggctg gctgatgccc atgctgttca gcagctcgcg ctcgttgtag atgtcgttag
     2641 ctggggccac agccacaccg atgaacaggg cacccaacac gggcatgaag aactgcaagc
     2701 tattctcgct gcacaccacg atccgatggt ttgtattcag cccatagcgc ttcatagctt
     2761 ctgccagccg aacgctcatc tcgaagtact cggcgtaggt aatgtccacc tcgatatgtg
     2821 cgtcggtaaa ggcgatggtg ccgggcacca gggcgtagcg cttcatggct ttgtgcagct
     2881 gctcgccggc ggtcccgtct tcgagtgggt agaatggcgc tgggcccttc ttaatgtttt
     2941 tggcatcttc catggtggct ttaccaacag taccggattg ccaagctgga agtcgagctt
     3001 ccattatata ccctctagtg tctaagcttg gccgccgagg ccagatcttg atatcctcga
     3061 ggaaattgga aggaaaatga ggtttaattg gaattttata tgcattagtg tattttttat
     3121 ttcaaaattt tagacaaata tttttggttt tcagttaagt acatttacat tcagatagac
     3181 cgaagaatat tggtttacta tataattagt tttagtaaat agcataattg agattaaaca
     3241 ctaaatggca atgtataaag actcccctct cagctatgta agacatttac cagaagaaaa
     3301 aacaaccaat aataacctat ttataaaaat aagtatctaa gataataaaa cattagaact
     3361 gtagaaaaga gaagaaattt acacatttta ggaatgaaat tgaataaagg tcatcaaata
     3421 ttttgaaatg acagatgaat caaactcagc atatttgtgt caaatggaat gtgttcatca
     3481 aacagcatga atctccgcat gaatctccgc atgatctcgg gggaaacagg tatttgcttc
     3541 ctcgcccaca tggaatgaat ccaggaccct ccagtgttcc tgaccatggg tgtgtctcct
     3601 gcttaagtca aggcttcctg ttttagagac agctgtggcc tttgctttcc atctgtcagg
     3661 taggaagtaa aaattgtccc tcagggacag ttcttggacg atacacttaa cacttcacct
     3721 atttcccact ggctataatt ttgttgcaca gctcctctat ctactgaaga aacaagtaca
     3781 cacattcctt ttgtgtttcc cctagataat cagctactcc tttaacactg aaagataatt
     3841 gcctttggaa aaaatctggg ttgaaggtca gtcagagttc ctgatgtctc gaagactttt
     3901 tttaaaattt tttttattag gtatttattt catttacatt tccaatgcta tcccaaaagt
     3961 cccacacaca ttctcccacc caatccctta cccacccact cccacttctt ggccctggcg
     4021 ctcccctgta ctgaggcata taaagttaac tttttttttt gtcatcttta atagtgatag
     4081 attacatata taccaaaaat tgtttttacc aaaaataaat ctctttatga ataatgatca
     4141 gccaatgttt gatttatgtg cctatctttt agaactatat actcaggatg atataaaaat
     4201 ttctcagctc aagagagtca caggtagaaa aagcctatga agcccactct gaggaaaagt
     4261 ccaaagtatg aaccactgac ggagagaaac tgaagcctcc actcaggcag caatgccgtg
     4321 aggagtgcag cagagtcaca ggtgctgggg ccttccagtt tccaaggctc cttccttcct
     4381 gttcttacaa atctgcagac tcaaagctgg agagagaaca attggtaacg tacatgtcac
     4441 acaagacgag aacccaagtc aactctccag attccaaggt tttaagaaaa tccaggtgaa
     4501 gggctagaga gacagataat tgctcctctt gcagaactga actgagttca gttcccaaca
     4561 cttacctgag gtggcttccg actacctgta actccgctgg catctaacac ctctgctctt
     4621 tttttttttt ttcaatttag ctattttttt taattaggta ttttcctcgt ttacattttc
     4681 aatgctatcc caaaggtccc ccatacccac ccatcccccc caatccccta cccacccact
     4741 cccccttttt ggccctggcg gttcccctgt actggggcat ataaagtttg caagtccaat
     4801 gggcctctct ttccagtgat ggccggtacc ggccagttag gccagagaaa tgttctggca
     4861 cctgcacttg cactggggac agcctatttt gctagtttgt tttgtttcgt tttgttttga
     4921 tggagagcgt atgttagtac tatcgattca cacaaaaaac caacacacag atgtaatgaa
     4981 aataaagata ttttattgcg gcctgtccaa tacctcccgt accttaatat tacttactta
     5041 tccttgagag acgtactagt aaccctgata aatgcttcaa taatattgaa aaaggacgag
     5101 tatgagcatc caacattttc gtgtcgcact cattcccttc tttgcggcat tttgcttgcc
     5161 tgtttttgca caccccgaaa cgctggtgaa agtaaaagat gctgaagatc aactgggtgc
     5221 aagagtgggc tatatcgaac tggatctcaa tagcggcaag atccttgagt ctttccgccc
     5281 cgaagaacga ttcccgatga tgagcacttt caaagtactg ctatgtggcg cggtgttgtc
     5341 ccgtatagac gccgggcaag agcagcttgg tcgccgtata cactactcac aaaacgactt
     5401 ggttgagtac tcgccggtca cggaaaagca tcttacggat ggcatgacgg taagagaatt
     5461 gtgtagtgct gccattacca tgagcgacaa caccgcggcc aacttacttc tgacaacgat
     5521 cggaggccct aaggagctga ctgcatttct tcataatatg ggtgatcatg tgacccggct
     5581 tgaccgctgg gaaccagagt tgaacgaagc cataccgaac gacgagcgtg ataccacgat
     5641 gccagtagca atggccacaa ctcttcggaa actactcact ggcgaacttc ttactctagc
     5701 atcacgacag cagctcatag actggatgga ggcggacaaa gtagcaggac cacttcttcg
     5761 ctcggccctc cctgctggct ggttcattgc tgacaaatcg ggggccggtg aacgcggctc
     5821 tcgcggcatc attgctgcgc tggggcctga tggtaagccc tcacgaatcg tagtgatcta
     5881 cacgacgggg agtcaggcca ctatggacga acgaaatagg cagatcgctg agatcggtgc
     5941 ctcactgatc aagcactggt aaccactgca gtggtttagc atttgcggcc gctgtcagac
     6001 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6061 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6121 cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
