LOCUS       sequence-306227-        8567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, 
            Betzig E, Meyer T
  TITLE     Engulfed cadherin fingers are polarized junctional structures 
            between collectively migrating endothelial cells.
  JOURNAL   Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub
            2016 Nov 14.
  PUBMED    27842057
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3438"; 
            journalName: "Nat Cell Biol"; date: "2016-12"; volume: "18"; issue: 
            "12"; pages: "1311-1323"
COMMENT     Sequence Label: pLV-EF1a-IRES-Blast
FEATURES             Location/Qualifiers
     source          1..8567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(762..778)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(762..778)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(775..797)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    786..802
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(810..816,817..834,835..840))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    855..876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(993..1010)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1164..1752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1244..1263)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1923..2714,2715..2783))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2546..2565
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2784..2888)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    2936..3070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2973..2992)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3027..3046
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3238..3871
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3918..4043
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4540..4773
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4958..5002
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5265..5380
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        5458..5669
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          5670..6608
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6565..6585
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6769..7318
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6913..6930)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7140..7159
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    7773..8361
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7826..7846)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8244..8255)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
      661 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag
      721 cgtttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
      781 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      841 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      901 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      961 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1021 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1081 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1141 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1201 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1261 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1321 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1381 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1441 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1501 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1561 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1621 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1681 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1741 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1801 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1861 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1921 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1981 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2041 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2101 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2161 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2221 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2281 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2341 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2401 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2461 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2521 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     2581 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     2641 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2701 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2761 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2821 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2881 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt
     2941 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3001 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     3061 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     3121 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     3181 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     3241 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     3301 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     3361 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     3421 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     3481 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     3541 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     3601 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     3661 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     3721 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     3781 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     3841 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     3901 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     3961 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     4021 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     4081 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     4141 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     4201 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     4261 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     4321 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     4381 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     4441 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     4501 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     4561 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     4621 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     4681 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     4741 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     4801 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     4861 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     4921 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     4981 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     5041 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     5101 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     5161 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     5221 agacagatcc attcgattag tgaacggatc tcgacggtat cgcctttaaa agaaaagggg
     5281 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     5341 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     5401 gcagagatcc agtttatcga tgaggccctt tcgtcttcac tcgaggtgcc cgtcagtggg
     5461 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     5521 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     5581 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     5641 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     5701 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     5761 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     5821 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     5881 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     5941 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     6001 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     6061 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     6121 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     6181 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     6241 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     6301 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     6361 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     6421 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     6481 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     6541 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     6601 catttcaggt gtcgtgagga tccgcggccg cgccggctct agatcgcgaa cgcgtgaatt
     6661 cctcgagggc ggccgctcta gagtcgacgg gccgcggtaa caattgttaa ctaacttaag
     6721 ctagcaacgg tttccctcta gcgggatcaa ttccgccccc cccccctaac gttactggcc
     6781 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     6841 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     6901 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     6961 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     7021 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     7081 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     7141 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     7201 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     7261 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc
     7321 atggtcatga aaacatttaa catttctcaa caagatctag aattagtaga agtagcgaca
     7381 gagaagatta caatgcttta tgaggataat aaacatcatg tgggagcggc aattcgtacg
     7441 aaaacaggag aaatcatttc ggcagtacat attgaagcgt atataggacg agtaactgtt
     7501 tgtgcagaag ccattgcgat tggtagtgca gtttcgaatg gacaaaagga ttttgacacg
     7561 attgtagctg ttagacaccc ttattctgac gaagtagata gaagtattcg agtggtaagt
     7621 ccttgtggta tgtgtaggga gttgatttca gactatgcac cagattgttt tgtgttaata
     7681 gaaatgaatg gcaagttagt caaaactacg attgaagaac tcattccact caaatatacc
     7741 cgaaattaac ccgggcgggg cgcgtctgga acaatcaacc tctggattac aaaatttgtg
     7801 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7861 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7921 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7981 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     8041 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     8101 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     8161 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     8221 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     8281 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     8341 ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg
     8401 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     8461 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     8521 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggac
//
