LOCUS       sequence-306236-        6273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Carroll P, Schreuder LJ, Muwanguzi-Karugaba J, Wiles S, Robertson 
            BD, Ripoll J, Ward TH, Bancroft GJ, Schaible UE, Parish T
  TITLE     Sensitive detection of gene expression in mycobacteria under 
            replicating and non-replicating conditions using optimized far-red 
            reporters.
  JOURNAL   PLoS One. 2010  . 5(3):e9823.
  PUBMED    20352111
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(3):e9823."
COMMENT     Sequence Label: pCHERRY3
FEATURES             Location/Qualifiers
     source          1..6273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1028..1044)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1029..1045)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     rep_origin      5244..5832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5733..5752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5986..6003
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6120..6141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6156..6161,6162..6179,6180..6186)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6194..6210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6199..6221
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6218..6234
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6218..6234
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tctagaggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct ctgggagtac ccgtgtgtac
      241 gaccagcacg gcatacatca tttcgacgcc gagagattcg ccgcccgaaa tgagcacgat
      301 ccggatccat ggtctcgaag ggcgaggagg acaacatggc gatcatcaag gagttcatgc
      361 gcttcaaggt ccacatggag ggctcggtca acggccacga gttcgagatc gagggcgagg
      421 gcgagggccg cccgtacgag ggcacccaga ccgccaagct gaaggtcacc aagggcggcc
      481 cgctgccgtt cgcctgggac atcctgtcgc cgcagttcat gtacggcagc aaggcctacg
      541 tcaagcaccc ggccgacatc ccggactacc tgaagctgtc gttcccggag ggcttcaagt
      601 gggagcgcgt catgaacttc gaggacggcg gcgtcgtcac cgtcacccag gactcgtcgc
      661 tgcaggacgg cgagttcatc tacaaggtca agctgcgggg caccaacttc ccgtcggacg
      721 gcccggtcat gcagaagaag accatgggct gggaggcctc gtcggagcgc atgtacccgg
      781 aggacggcgc cctgaagggc gagatcaagc agcggctgaa gctgaaggac ggcggccact
      841 acgacgccga ggtcaagacc acctacaagg ccaagaagcc ggtccagctg ccgggcgcct
      901 acaacgtgaa catcaagctg gacatcacca gccacaacga ggactacacc atcgtcgagc
      961 agtacgagcg cgccgagggc cgccacagca ccggcggcat ggacgagctg tacaagtgaa
     1021 agcttatcga taccgtcgac ctcgaggggg ggcccccggg gatcatcgag ccgagaacgt
     1081 tatcgaagtt ggtcatgtgt aatcccctcg tttgaacttt ggattaagcg tagatacacc
     1141 cttggacaag ccagttggat tcggagacaa gcaaattcag ccttaaaaag ggcgaggccc
     1201 tgcggtggtg gaacaccgca gggcctctaa ccgctcgacg cgctgcacca accagcccgc
     1261 gaacggctgg cagccagcgt aaggcgcggc tcatcgggcg gcgttcgcca cgatgtcctg
     1321 cacttcgagc caagcctcga acacctgctg gtgtgcacga ctcacccggt tgttgacacc
     1381 gcgcgcggcc gtgcgggctc ggtggggcgg ctctgtcgcc cttgccagcg tgagtagcgc
     1441 gtacctcacc tcgcccaaca ggtcgcacac agccgattcg tacgccataa agccaggtga
     1501 gcccaccagc tccgtaagtt cgggcgctgt gtggctcgta cccgcgcatt caggcggcag
     1561 ggggtctaac gggtctaagg cggcgtgtac ggccgccaca gcggctctca gcggcccgga
     1621 aacgtcctcg aaacgacgca tgtgttcctc ctggttggta caggtggttg ggggtgctcg
     1681 gctgtcgctg gtgttccacc accagggctc gacgggagag cgggggagtg tgcagttgtg
     1741 gggtggcccc tcagcgaaat atctgacttg gagctcgtgt cggaccatac accggtgatt
     1801 aatcgtggtc tactaccaag cgtgagccac gtcgccgacg aatttgagca gctctggctg
     1861 ccgtactggc cgctggcaag cgacgatctg ctcgagggga tctaccgcca aagccgcgcg
     1921 tcggccctag gccgccggta catcgaggcg aacccaacag cgctggcaaa cctgctggtc
     1981 gtggacgtag accatccaga cgcagcgctc cgagcgctca gcgcccgggg gtcccatccg
     2041 ctgcccaacg cgatcgtggg caatcgcgcc aacggccacg cacacgcagt gtgggcactc
     2101 aacgcccctg ttccacgcac cgaatacgcg cggcgtaagc cgctcgcata catggcggcg
     2161 tgcgccgaag gccttcggcg cgccgtcgac ggcgaccgca gttactcagg cctcatgacc
     2221 aaaaaccccg gccacatcgc ctgggaaacg gaatggctcc actcagatct ctacacactc
     2281 agccacatcg aggccgagct cggcgcgaac atgccaccgc cgcgctggcg tcagcagacc
     2341 acgtacaaag cggctccgac gccgctaggg cggaattgcg cactgttcga ttccgtcagg
     2401 ttgtgggcct atcgtcccgc cctcatgcgg atctacctgc cgacccggaa cgtggacgga
     2461 ctcggccgcg cgatctatgc cgagtgccac gcgcgaaacg ccgaattccc gtgcaacgac
     2521 gtgtgtcccg gaccgctacc ggacagcgag gtccgcgcca tcgccaacag catttggcgt
     2581 tggatcacaa ccaagtcgcg catttgggcg gacgggatcg tggtctacga ggccacactc
     2641 agtgcgcgcc agtcggccat ctcgcggaag ggcgcagcag cgcgcacggc ggcgagcaca
     2701 gttgcgcggc gcgcaaagtc cgcgtcagcc atggaggcat tgctatgagc gacggctaca
     2761 gcgacggcta caaccggcag ccgactgtcc gcaaaaagcg gcgcgtgacc gccgccgaag
     2821 gcgctcgaat caccggacta tccgaacgcc acgtcgtccg gctcgtggcg caggaacgca
     2881 gcgagtggct cgccgagcag gctgcacgcc gcgaacgcat ccgcgcctat cacgacgacg
     2941 agggccactc ttggccgcaa acggccaaac atttcgggct gcatctggac accgttaagc
     3001 gactcggcta tcgggcgagg aaagagcgtg cggcagaaca ggaagcggct caaaaggccc
     3061 acaacgaagc cgacaatcca ccgctgttct aacgcaattg gggagcgggt gtcgcggggg
     3121 ttccgtgggg ggttccgttg caacgggtcg gacaggtaaa agtcctggta gacgctagtt
     3181 ttctggtttg ggccatgcct gtctcgttgc gtgtttcgtt gcgcccgttt tgaataccag
     3241 ccagacgaga cggggttcta cgaatcttgg tcgataccaa gccatttccg ctgaatatcg
     3301 gggagctcac cgccagaatc ggtggttgtg gtgatgtacg tggcgaactc cgttgtagtg
     3361 cctgtggtgg catccgtggc cactctcgtt gcacggttcg ttgtgccgtt acaggccccg
     3421 ttgacagctc accgaacgta gttaaaacat gctggtcaaa ctaggtttac caacgatacg
     3481 agtcagctca tctagggcca gttctaggcg ttgttcgttg cgcggttcgt tgcgcatgtt
     3541 tcgtgtggtt gctagatggc tccgcaacca cacgcttcga ggttgagtgc ttccagcacg
     3601 ggcgcgatcc agaagaactt cgtcgtgcga ctgtcctcgt tgatccttgc cgagctggga
     3661 tggaagcccg gccgacccac cctggaggag atgatcgagg atgccagggc ctttcacgcc
     3721 cgccgctgct gagcgtccgc cgccgggccc gcaccgccgt cggccggccc gctccgggct
     3781 cgcagcagcg ggcttcggcg cgggcccggg gctcccgggc cgccgggcgg ggctccgccc
     3841 ggcggccgcc gggggccggg ggcggcgccg ggcggcccgg ggcgtcaggc gccgggggcg
     3901 gtgtccggcg gcccccagag gaactgcgcc agttcctccg gatcggtgaa gccggagaga
     3961 tccagcgggg tctcctcgaa cacctcgaag tcgtgcagga aggtgaaggc gagcagttcg
     4021 cgggcgaagt cctcggtccg cttccactgc gccccgtcga gcagcgcggc caggatctcg
     4081 cggtcgcccc ggaaggcgtt gagatgcagt tgcaccaggc tgtagcggga gtctcccgca
     4141 tagacgtcgg tgaagtcgac gatcccggtg acctcggtcg cggccaggtc cacgaagatg
     4201 ttggtcccgt gcaggtcgcc gtggacgaac cggggttcgc ggccggccag cagcgtgtcc
     4261 acgtccggca gccagtcctc caggcggtcc agcagccggg gcgagaggta gccccacccg
     4321 cggtggtcct cgacggtcgc cgcgcggcgt tcccgcagca gttccgggaa gacctcggaa
     4381 tggggggtga gcacggtgtt cccggtcagc ggcaccctgt gcagccggcc gagcacccgg
     4441 ccgagttcgc gggccagggc gagcagcgcg ttccggtcgg tcgtgccgtc catcgcggac
     4501 cgccaggtgg tgccggtcat ccggctcatc accaggtagg gccacggcca ggctccggtg
     4561 ccgggccgca gctcgccgcg gccgaggagg cggggcaccg gcaccggggc gtccgccagg
     4621 accgcgtacg cctccgactc cgacgcgagg ctctccggac cgcaccagtg ctcgccgaac
     4681 agcttgatca ccgggccggg ctcgccgacc agtacggggt tggtgctctc gccgggcacc
     4741 cgcagcaccg gcggcaccgg cagcccgagc tcctccaggg ctcggcgggc cagcggctcc
     4801 cagaattcct ggtcgttccg caggctcgcg taggaatcat ccgaatcaat acggtcgaga
     4861 agtaacaggg attcttgtgt cacagcggac ctctattcac agggtacggg ccggcttaat
     4921 tccgcacggc cggtcgcgac acggcctgtc cgcaccgcgg atcaggcgtt gacgatgacg
     4981 ggctggtcgg ccacgtcggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5041 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5101 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5161 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5221 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5281 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5341 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5401 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5461 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5521 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5581 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5641 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5701 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5761 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5821 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5881 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5941 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6001 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     6061 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     6121 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     6181 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt
     6241 accagatctg gctcgcaccg cggtggcggc cgc
//
