LOCUS       sequence-306238-        7424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein 
            (Spike) for VSV pseudotyping.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7424)
  TITLE     Truncated SARS-CoV-2 Spike protein constructs for making VSV pseudo 
            virus
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4137382
FEATURES             Location/Qualifiers
     source          1..7424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        136..515
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        516..719
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     670..690
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(4577..4596)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4642..4697
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4696..4715)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5058..5074)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5058..5074)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5071..5093)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5082..5098
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5106..5112,5113..5130,5131..5136))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5151..5172
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5231..5426
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5277..5412
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5432..5566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5469..5488)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5523..5542
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5634..5651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5805..6393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5885..5904)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(6564..6632,6633..7424)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6782..6801)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca attacggggt
      181 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      241 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      301 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      361 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      421 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      481 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      541 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      601 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      661 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg
      721 gtttagtgaa ccgtcagatc agatctttgt cgatcctacc atccactcga cacacccgcc
      781 agcggccgcc accatgttcg tctttcttgt gttactcccc ctggtttcgt cccagtgcgt
      841 gaacttgact acgcgaaccc aactgccgcc agcatataca aatagtttca cccgtggagt
      901 atactaccct gataaagtgt ttagatcaag cgtactacac tctactcagg acctgtttct
      961 ccccttcttc tccaatgtca catggtttca tgcgatccac gttagcggca ccaacgggac
     1021 aaagcgcttt gacaaccctg tgctgccatt caatgatggt gtgtattttg cttctaccga
     1081 gaagagcaac ataattcggg gctggatctt cggaactacc ctcgacagta aaactcagtc
     1141 attgctgatt gtgaataacg ccacaaatgt cgttatcaag gtctgtgaat ttcaattctg
     1201 caacgatcca ttccttgggg tgtattacca taaaaataat aagtcctgga tggagagcga
     1261 atttagggta tactcgtctg ccaacaattg tactttcgag tatgtgtctc agcccttctt
     1321 aatggatctg gaaggtaaac agggcaactt taagaacctc cgcgagtttg tattcaaaaa
     1381 tatagacgga tactttaaga tttattccaa gcacacacca atcaatttgg tgcgggatct
     1441 cccgcaaggg ttcagcgctc tggaacctct agtggacctc cccatcggca ttaacatcac
     1501 aagattccag acgctgttag ccctgcatag atcatacctt actcccggtg acagtagttc
     1561 tgggtggacc gcaggcgcgg ctgcatatta cgtgggatat ttgcagcctc ggacgtttct
     1621 gctgaaatac aacgagaacg gcaccattac agatgccgtg gattgcgccc ttgaccccct
     1681 aagcgaaact aagtgtacgc ttaaaagctt caccgtcgag aagggaattt accagacttc
     1741 caactttagg gtccagccga cagaatcaat agttaggttc ccaaatatca ccaatttgtg
     1801 cccttttggg gaagtcttca atgctacacg atttgcttcg gtttatgcct ggaacaggaa
     1861 gcgtatctca aattgcgtcg cggactacag cgtgctctat aatagtgcaa gtttctcgac
     1921 ctttaaatgt tatggcgtgt ccccgaccaa actaaatgac ctctgtttta caaacgttta
     1981 cgccgattct tttgtgattc gcggtgatga ggtacgccaa atcgcaccgg gccagactgg
     2041 caagattgcc gattacaact acaagttgcc tgacgacttc accggatgtg ttatagcctg
     2101 gaatagcaat aatctggatt ctaaggtcgg aggtaactat aactacctgt atagactgtt
     2161 ccgtaagtca aacctgaaac cctttgagcg ggacatctcc acagagattt accaagcagg
     2221 gtctaccccc tgcaatgggg ttgaaggatt caattgctac tttccattac agagctacgg
     2281 gttccagcct acgaacgggg ttggttacca gccttatagg gtagtggtgc tgtcgttcga
     2341 actgttgcac gctccagcta cagtgtgcgg gcctaaaaaa tcaactaacc tggttaaaaa
     2401 taagtgtgtt aattttaact tcaatggctt aaccggaact ggagtgctca cggagtcaaa
     2461 caagaaattt ctaccgtttc agcaattcgg gagggatatc gcagacacta cggacgcggt
     2521 cagagatcca cagacccttg agatccttga cataacaccc tgctctttcg gaggtgtctc
     2581 cgtaattaca ccaggcacta atacctctaa tcaggtggcc gtgctttatc aggatgttaa
     2641 ctgtacagag gtcccggtgg ctatccatgc cgaccaattg acacctacgt ggcgcgtcta
     2701 ttctaccggc tccaacgtct tccagacccg ggctggatgc ttgataggcg cagaacacgt
     2761 aaacaactct tacgaatgtg atattcccat tggagccggg atctgcgcaa gctaccagac
     2821 ccaaacaaac agtcccagac gggctcgtag cgtggcctct cagagcataa tagcctatac
     2881 catgtccctt ggcgcggaga atagtgtggc atactcaaat aatagcatcg ctatccccac
     2941 taactttact atttccgtga cgactgagat tctgccagtg tctatgacta agacatccgt
     3001 ggattgcaca atgtatattt gtggggattc cacagagtgt agcaacttgt tgctgcaata
     3061 tggatcattc tgtacgcagc tgaatcgcgc cctcaccggc atcgctgtgg aacaggacaa
     3121 gaacactcaa gaggtctttg ctcaggtgaa gcaaatctac aagaccccac ctataaaaga
     3181 cttcgggggt tttaacttct cgcagattct ccccgacccc agcaagcctt caaaaaggag
     3241 ctttattgag gacctgttgt tcaacaaggt tactttagcc gatgccggtt ttatcaaaca
     3301 gtacggcgac tgtcttggcg atatagcagc ccgggatctg atttgcgcac aaaaattcaa
     3361 tggccttacc gtactgccac cccttctcac cgacgaaatg atcgcgcagt acacctccgc
     3421 actgctggct gggaccatca cttcgggatg gacctttggg gctggtgctg ctttgcaaat
     3481 tcccttcgcc atgcagatgg cctatcgatt caatggcata ggggtaacac agaacgtatt
     3541 gtatgaaaat caaaagctaa tcgctaacca gtttaattca gccattggta agatccagga
     3601 ttcactctcc agtacagcct ctgccctggg aaagctccag gacgtggtca accagaacgc
     3661 gcaggcattg aatacactcg tcaaacagct gagtagcaat ttcggcgcaa tcagtagtgt
     3721 cttaaacgat atactgagta ggcttgataa agtagaggcc gaggtccaga ttgacagact
     3781 aatcacgggg cgtcttcaga gtttacagac ctatgtgacc caacagctga tccgagcagc
     3841 ggaaattcga gcaagcgcga acctggccgc tactaaaatg agtgagtgcg tgctgggcca
     3901 aagtaaacga gtcgactttt gcggcaaagg gtatcatctc atgtctttcc ctcagtccgc
     3961 ccctcacggt gttgttttcc tccacgtgac atacgttccc gcgcaggaaa agaattttac
     4021 tacggcaccc gctatttgcc atgacggaaa ggctcatttt ccacgggagg gagtgttcgt
     4081 ttcaaacggg acacactggt ttgtaactca gcggaacttt tatgagcctc aaatcatcac
     4141 gacagacaat accttcgtga gtggcaactg cgatgtagtc ataggcattg tgaataacac
     4201 tgtttatgat ccacttcagc cggaactcga cagcttcaaa gaggaattgg ataagtactt
     4261 taaaaatcat accagtccag acgtagatct cggcgatata tcgggcatta acgccagtgt
     4321 ggtcaatata caaaaggaga ttgacagact gaatgaagta gctaagaacc tcaacgaatc
     4381 tctgattgac ctgcaagagc tagggaagta cgagcagtat atcaaatggc catggtacat
     4441 atggctgggt tttatcgcgg gtcttattgc catagtcatg gtgacgatta tgttgtgctg
     4501 tatgacctcc tgctgttctt gtttgaaagg ctgctgctcc tgtggtagct gttgctaatc
     4561 cggactcaga tctcgatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     4621 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     4681 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     4741 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     4801 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     4861 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     4921 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     4981 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca
     5041 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5101 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5161 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5221 cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     5281 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5341 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     5401 tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta
     5461 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5521 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga
     5581 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5641 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5701 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5761 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5821 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5881 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5941 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6001 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6061 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6121 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6181 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6241 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6301 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6361 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6421 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6481 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6541 tatgagtaaa cttggtctga cagatgagta ttcaacattt ccgtgtcgcc cttattccct
     6601 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6661 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6721 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6781 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6841 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6901 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6961 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7021 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7081 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7141 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7201 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7261 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7321 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7381 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaa
//
