LOCUS       sequence-306266-       10244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CasRx.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   He B, Peng W, Huang J, Zhang H, Zhou Y, Yang X, Liu J, Li Z, Xu C, 
            Xue M, Yang H, Huang P
  TITLE     Modulation of metabolic functions through Cas13d-mediated gene 
            knockdown in liver.
  JOURNAL   Protein Cell. 2020 Mar 18. pii: 10.1007/s13238-020-00700-2. doi: 
            10.1007/s13238-020-00700-2.
  PUBMED    32185621
REFERENCE   2  (bases 1 to 10244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Cell. 2020 Mar 18. pii: 10.1007/s13238-020-00700-2. doi: 
            10.1007/s13238-020-00700-2."
COMMENT     Sequence Label: PB-CAG-CasRX-P2A-EGFP-BGH PolyA
FEATURES             Location/Qualifiers
     source          1..10244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   250..284
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        585..964
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        966..1243
                     /label=chicken beta-actin promoter
     intron          1244..2260
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2184..2206
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             2311..2331
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2341..5238
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             5254..5274
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5281..5337
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5344..5346,5347..5349,5350..6063)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5344..5346,5347..5349,5350..6060)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5389..5410)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5650..5669)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5997..6018
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6115..6132)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6121..6345
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6530..6592
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6677..6696)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6678..6696)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6703..6720)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6703..6719)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6712..6734)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6860..7288
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6947..6966)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7157..7178
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             7632..8426
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7686..7705)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8296..8315
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(8444..8512,8513..9304)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8662..8681)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9475..10063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9964..9983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10217..10234
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccttaac cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg
      301 ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc
      361 ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa
      421 cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc
      481 atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat
      541 tttcttgtta tagatatcat taatatcgat ggcgcgccgg attcgacatt gattattgac
      601 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      901 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      961 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     1021 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     1081 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag
     1141 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     1201 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg
     1261 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
     1321 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
     1381 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
     1441 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
     1501 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
     1561 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
     1621 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     1681 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     1741 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     1801 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
     1861 ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     1921 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     1981 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     2041 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     2101 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     2161 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     2221 ctctgctaac catgttcatg ccttcttctt tttcctacag atccttaatt aagcacgcgt
     2281 gcgggatccg ctcaattcgc cgccaccatg cctaaaaaga aaagaaaggt gggttctggt
     2341 atcgagaaga agaagagctt cgccaagggc atgggagtga agagcaccct ggtgtccggc
     2401 tctaaggtgt acatgaccac atttgctgag ggaagcgacg ccaggctgga gaagatcgtg
     2461 gagggcgata gcatcagatc cgtgaacgag ggagaggctt tcagcgccga gatggctgac
     2521 aagaacgctg gctacaagat cggaaacgcc aagttttccc acccaaaggg ctacgccgtg
     2581 gtggctaaca acccactgta caccggacca gtgcagcagg acatgctggg actgaaggag
     2641 acactggaga agaggtactt cggcgagtcc gccgacggaa acgataacat ctgcatccag
     2701 gtcatccaca acatcctgga tatcgagaag atcctggctg agtacatcac aaacgccgct
     2761 tacgccgtga acaacatctc cggcctggac aaggatatca tcggcttcgg aaagttttct
     2821 accgtgtaca catacgacga gttcaaggat ccagagcacc accgggccgc ttttaacaac
     2881 aacgacaagc tgatcaacgc catcaaggct cagtacgacg agttcgataa ctttctggat
     2941 aaccccaggc tgggctactt cggacaggct ttcttttcta aggagggcag aaactacatc
     3001 atcaactacg gaaacgagtg ttacgacatc ctggccctgc tgagcggact gaggcactgg
     3061 gtggtgcaca acaacgagga ggagtctcgg atcagccgca cctggctgta caacctggac
     3121 aagaacctgg ataacgagta catctccaca ctgaactacc tgtacgacag gatcaccaac
     3181 gagctgacaa acagcttctc caagaactct gccgctaacg tgaactacat cgctgagacc
     3241 ctgggcatca acccagctga gttcgctgag cagtacttca gattttccat catgaaggag
     3301 cagaagaacc tgggcttcaa catcacaaag ctgagagaag tgatgctgga cagaaaggat
     3361 atgtccgaga tcaggaagaa ccacaaggtg ttcgattcta tcagaaccaa ggtgtacaca
     3421 atgatggact ttgtgatcta caggtactac atcgaggagg atgccaaggt ggccgctgcc
     3481 aacaagagcc tgcccgacaa cgagaagtct ctgagcgaga aggatatctt cgtgatcaac
     3541 ctgagaggct cctttaacga cgatcagaag gacgctctgt actacgatga ggccaacagg
     3601 atctggagaa agctggagaa catcatgcac aacatcaagg agttccgggg aaacaagacc
     3661 cgcgagtaca agaagaagga cgctccaagg ctgcctagga tcctgcctgc tggaagggac
     3721 gtgagcgcct tcagcaagct gatgtacgcc ctgacaatgt ttctggacgg aaaggagatc
     3781 aacgatctgc tgaccacact gatcaacaag ttcgacaaca tccagtcttt tctgaaagtg
     3841 atgcctctga tcggcgtgaa cgctaagttc gtggaggagt acgccttctt taaggacagc
     3901 gccaagatcg ctgatgagct gcggctgatc aagtcctttg ccaggatggg agagccaatc
     3961 gctgacgcta ggagagctat gtacatcgat gccatccgga tcctgggaac caacctgtct
     4021 tacgacgagc tgaaggctct ggccgacacc ttcagcctgg atgagaacgg caacaagctg
     4081 aagaagggca agcacggaat gcgcaacttc atcatcaaca acgtgatcag caacaagcgg
     4141 tttcactacc tgatcagata cggcgaccca gctcacctgc acgagatcgc taagaacgag
     4201 gccgtggtga agttcgtgct gggacggatc gccgatatcc agaagaagca gggccagaac
     4261 ggaaagaacc agatcgaccg ctactacgag acctgcatcg gcaaggataa gggaaagtcc
     4321 gtgtctgaga aggtggacgc tctgaccaag atcatcacag gcatgaacta cgaccagttc
     4381 gataagaaga gatctgtgat cgaggacacc ggaagggaga acgccgagag agagaagttt
     4441 aagaagatca tcagcctgta cctgacagtg atctaccaca tcctgaagaa catcgtgaac
     4501 atcaacgcta gatacgtgat cggcttccac tgcgtggagc gcgatgccca gctgtacaag
     4561 gagaagggat acgacatcaa cctgaagaag ctggaggaga agggctttag ctccgtgacc
     4621 aagctgtgcg ctggaatcga cgagacagcc cccgacaaga ggaaggatgt ggagaaggag
     4681 atggccgaga gagctaagga gagcatcgac tccctggagt ctgctaaccc taagctgtac
     4741 gccaactaca tcaagtactc cgatgagaag aaggccgagg agttcaccag gcagatcaac
     4801 agagagaagg ccaagaccgc tctgaacgcc tacctgagga acacaaagtg gaacgtgatc
     4861 atccgggagg acctgctgcg catcgataac aagacctgta cactgttccg gaacaaggct
     4921 gtgcacctgg aggtggctcg ctacgtgcac gcctacatca acgacatcgc cgaggtgaac
     4981 tcctactttc agctgtacca ctacatcatg cagaggatca tcatgaacga gagatacgag
     5041 aagtctagcg gcaaggtgtc tgagtacttc gacgccgtga acgatgagaa gaagtacaac
     5101 gatagactgc tgaagctgct gtgcgtgcct ttcggatact gtatcccacg gtttaagaac
     5161 ctgagcatcg aggccctgtt cgaccgcaac gaggctgcca agtttgataa ggagaagaag
     5221 aaggtgagcg gcaactccgg ttctggtctc gagcccaaga agaagaggaa agtcctcgag
     5281 gctactaact tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctatg
     5341 catatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     5401 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     5461 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     5521 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     5581 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     5641 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     5701 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     5761 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     5821 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     5881 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     5941 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     6001 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     6061 taaacgctag cgctgtcgac gccggttagg ggcccgttta aacccgctga tcagcctcga
     6121 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     6181 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     6241 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     6301 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcggcg cgcctaaaag
     6361 ttttgttact ttatagaaga aattttgagt ttttgttttt ttttaataaa taaataaaca
     6421 taaataaatt gtttgttgaa tttattatta gtatgtaagt gtaaatataa taaaacttaa
     6481 tatctattca aattaataaa taaacctcga tatacagacc gataaaacac atgcgtcaat
     6541 tttacgcatg attatcttta acgtacgtca caatatgatt atctttctag ggttaaggcc
     6601 ggccaataag ggcgaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc
     6661 tagagggccc aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca
     6721 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     6781 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     6841 cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6901 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6961 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7021 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7081 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7141 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7201 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7261 ctgatttaac aaaaatttaa cgcgaatttt aacaaaattc agggcgcaag ggctgctaaa
     7321 ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca
     7381 gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca
     7441 gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat
     7501 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     7561 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag
     7621 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     7681 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     7741 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     7801 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     7861 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     7921 tgccggggca ggatctcctg tcatcccacc ttgctcctgc cgagaaagta tccatcatgg
     7981 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     8041 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     8101 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     8161 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     8221 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     8281 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     8341 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     8401 atcgccttct tgacgagttc ttctgaattg aaaaaggaag agtatgagta ttcaacattt
     8461 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8521 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8581 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8641 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8701 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8761 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8821 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8881 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8941 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     9001 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     9061 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     9121 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     9181 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     9241 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     9301 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     9361 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9421 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9481 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9541 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9601 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9661 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9721 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9781 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9841 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9901 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9961 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    10021 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    10081 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    10141 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    10201 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
