LOCUS       sequence-306282-       11685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SARS-CoV-2 fluorescent reporter 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11685)
  AUTHORS   Abbott TR, Dhamdhere G, Liu Y, Lin X, Goudy L, Zeng L, Chemparathy 
            A, Chmura S, Heaton NS, Debs R, Pande T, Endy D, La Russa MF, Lewis 
            DB, Qi LS
  TITLE     Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 
            and Influenza.
  JOURNAL   Cell. 2020 May 14;181(4):865-876.e12. doi: 
            10.1016/j.cell.2020.04.020. Epub 2020 Apr 29.
  PUBMED    32353252
REFERENCE   2  (bases 1 to 11685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.020"; journalName: "Cell"; date: 
            "2020-05-14- 14"; volume: "181"; issue: "4"; pages: "865-876.e12"
COMMENT     Sequence Label: pSLQ5079_pHR_PGK_sfGFP_CoV-F1
FEATURES             Location/Qualifiers
     source          1..11685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2..18)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             108..341
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(366..384)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(367..384)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(674..693)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(907..926)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1026..1048
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1086..1104)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1172..1276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1277..1345,1346..2137)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1495..1514)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2308..2896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2797..2816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3050..3067
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3137..3157)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3142..3471
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3322..3457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4605..4670
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4800..4820
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5245..5379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5282..5301)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5336..5355
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5547..6180
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6227..6352
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6849..7082
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7267..7311
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7596..7713
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7791..8290
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7826..7849)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8223..8242
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(8347..8349,8350..9063)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             8347..9060
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(8377..8405)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     9000..9020
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    11095..11683
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11148..11168)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11566..11577)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
       61 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
      121 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
      181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      301 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
      361 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
      421 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
      481 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
      541 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
      601 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      661 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      721 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      781 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      841 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
      901 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      961 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     1021 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     1081 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     1141 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1201 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1261 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1321 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1381 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1441 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1501 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1561 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1621 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1681 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1741 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1801 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1861 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1921 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1981 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2041 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2101 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2161 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2221 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2281 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2341 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2401 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2461 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2521 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2581 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2641 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2701 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2761 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2821 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2881 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2941 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3001 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3061 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gtcggccgat
     3121 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     3181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     3241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     3301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     3361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     3421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga
     3481 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
     3541 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
     3601 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     3661 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     3721 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     3781 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     3841 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     3901 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
     3961 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
     4021 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag
     4081 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     4141 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
     4201 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
     4261 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
     4321 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
     4381 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt
     4441 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg
     4501 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg
     4561 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     4621 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     4681 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     4741 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     4801 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     4861 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     4921 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     4981 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     5041 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     5101 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     5161 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     5221 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     5281 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5341 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     5401 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     5461 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     5521 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
     5581 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     5641 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     5701 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     5761 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     5821 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     5881 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     5941 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     6001 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6061 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6121 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6181 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     6241 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     6301 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     6361 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     6421 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     6481 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     6541 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     6601 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     6661 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
     6721 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     6781 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     6841 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     6901 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     6961 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     7021 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     7081 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     7141 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     7201 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     7261 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     7321 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     7381 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     7441 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     7501 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     7561 cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     7621 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     7681 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     7741 ccagtttgga tcgataagct tgatatctaa ttcctgcagc caattctacc gggtagggga
     7801 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     7861 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     7921 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     7981 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     8041 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     8101 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     8161 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     8221 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     8281 gggcctttcg acctgcagcc caagcttggg gatctggagc tctcgagaat tctcacgcgt
     8341 gccaccatga gcaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
     8401 gatggtgatg ttaatgggca caaattttct gtccgtggag agggtgaagg tgatgctaca
     8461 aacggaaaac tcacccttaa atttatttgc actactggaa aactacctgt tccgtggcca
     8521 acacttgtca ctactctgac ctatggtgtt caatgctttt cccgttatcc ggatcacatg
     8581 aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga acgcactata
     8641 tctttcaaag atgacgggac ctacaagacg cgtgctgaag tcaagtttga aggtgatacc
     8701 cttgttaatc gtatcgagtt aaagggtatt gattttaaag aagatggaaa cattcttgga
     8761 cacaaactcg agtacaactt taactcacac aatgtataca tcacggcaga caaacaaaag
     8821 aatggaatca aagctaactt caaaattcgc cacaacgttg aagatggttc cgttcaacta
     8881 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     8941 cattacctgt cgacacaatc tgtcctttcg aaagatccca acgaaaagcg tgaccacatg
     9001 gtccttcttg agtttgtaac tgctgctggg attacacatg gcatggatga gctctacaaa
     9061 taacttcagt cagctgatgc acaatcgttt ttaaacgggt ttgcggtgta agtgcagccc
     9121 gtcttacacc gtgcggcaca ggcactagta ctgatgtcgt atacagggct tttgacatct
     9181 acaatgataa agtagctggt tttgctaaat tcctaaaaac taattgttgt cgcttccaag
     9241 aaaaggacga agatgacaat ttaattgatt cttactttgt agttaagaga cacactttct
     9301 ctaactacca acatgaagaa acaatttata atttacttaa ggattgtcca gctgttgcta
     9361 aacatgactt ctttaagttt agaatagacg gtgacatggt accacatata tcacgtcaac
     9421 gtcttactaa atacacaatg gcagacctcg tctatgcttt aaggcatttt gatgaaggta
     9481 attgtgacac attaaaagaa atacttgtca catacaattg ttgtgatgat gattatttca
     9541 ataaaaagga ctggtatgat tttgtagaaa acccagatat attacgcgta tacgccaact
     9601 taggtgaacg tgtacgccaa gctttgttaa aaacagtaca attctgtgat gccatgcgaa
     9661 atgctggtat tgttggtgta ctgacattag ataatcaaga tctcaatggt aactggtatg
     9721 atttcggtga tttcatacaa accacgccag gtagtggagt tcctgttgta gattcttatt
     9781 attcattgtt aatgcctata ttaaccttga ccagggcttt aactgcagag tcacatgttg
     9841 acactgactt aacaaagcct tacattaagt gggatttgtt aaaatatgac ttcacggaag
     9901 agaggttaaa actctttgac cgttatttta aatattggga tcagacatac cacccaaatt
     9961 gtgttaactg tttggatgac agatgcattc tgcattgtgc aaactttaat gttttattct
    10021 ctacagtgtt cccacctaca agttttggac cactagtgag aaaaatattt gttgatggtg
    10081 ttccatttgt agtttcaact ggataccact tcagagagct aggtgttgta cataatcagg
    10141 atgtaaactt acatagctct agacttagtt ttaaggaatt acttgtgtat gctgctgacc
    10201 ctgctatgca cgctgcttct ggtaatctat tactagataa acgcactacg tgcttttcag
    10261 tagctgcact tactaacaat gttgcttttc aaactgtcaa acccggtaat tttaacaaag
    10321 acttctatga ctttgctgtg tctaagggtt tctttaagga aggaagttct gttgaattaa
    10381 aacacttctt ctttgctcag gatggtaatg ctgctatcag cgattatgac tactatcgtt
    10441 ataatctacc aacaatgtgt gatatcagac aactactatt tgtagttgaa gttgttgata
    10501 agtactttga ttgttacgat ggtggctgta ttaatgctaa ccaagtcatc gtcaacaacc
    10561 tagacaaatc agctggtttt ccatttaata aatggggtaa ggctagactt tattatgatt
    10621 caatgagtta tgaggatcaa gatgcacttt tcgcatatac aaaacgtaat gtcatcccta
    10681 ctataactca aatgaatctt aagtatgcca ttagtgcaaa gaatagagct cgcaccgtag
    10741 ctggtgtctc tatctgtagt actatgacca atagacagtt tcatcaaaaa ttattgaaat
    10801 caatagccgc cactagagga gctactgtag taattggaac aagcaaattc tatggtggtt
    10861 ggcacaacat gttaaaaact gtttatagtg atgtagaaaa ccctcacctt atgggttggg
    10921 attatcctaa atgtgataga gccatgccta acatgcttag aattatggcc tcacttgttc
    10981 ttgctcgcaa acatacaacg tgttgtagct tgtcacaccg tttctataga ttagctaatg
    11041 agtgtgctca agtattgagt gaaggcatgc aagcttgata tcaagcttat cgataatcaa
    11101 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
    11161 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
    11221 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
    11281 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
    11341 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
    11401 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
    11461 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
    11521 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
    11581 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
    11641 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcat
//
