LOCUS       sequence-306295-       10765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors
            with a constitutive mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10765)
  AUTHORS   Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim 
            WA
  TITLE     Engineering Customized Cell Sensing and Response Behaviors Using 
            Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. 
            Epub 2016 Jan 28.
  PUBMED    26830878
REFERENCE   2  (bases 1 to 10765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.01.012"; journalName: "Cell"; date: 
            "2016-02-11- 11"; volume: "164"; issue: "4"; pages: "780-91"
COMMENT     Sequence Label: pHR_Gal4UAS_tBFP_PGK_mCherry
FEATURES             Location/Qualifiers
     source          1..10765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          455..520
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             650..670
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1095..1229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1132..1151)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1186..1205
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1397..2030
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2077..2202
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2699..2932
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3117..3161
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3446..3563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     3789..3813
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3894..4595
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        4718..5217
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4753..4776)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5150..5169
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5238..5948
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5383..5401)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5651..5670
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6025..6613
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6078..6098)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6496..6507)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6616..6632)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6617..6633)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6723..6956
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6981..6999)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6982..6999)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7289..7308)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7522..7541)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7641..7663
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7701..7719)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7787..7891
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7892..7960,7961..8752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8110..8129)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8923..9511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9412..9431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9665..9682
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9752..9772)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9757..10086
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9937..10072
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
       61 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
      121 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
      181 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
      241 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
      301 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
      361 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
      421 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
      481 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
      541 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
      601 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
      661 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
      721 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
      781 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
      841 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
      901 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
      961 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     1021 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     1081 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     1141 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     1201 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     1261 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     1321 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     1381 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     1441 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     1501 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     1561 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     1621 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     1681 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     1741 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     1801 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     1861 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     1921 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     1981 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     2041 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     2101 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     2161 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     2221 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     2281 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     2341 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     2401 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     2461 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     2521 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     2581 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     2641 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     2701 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     2761 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     2821 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     2881 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     2941 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     3001 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     3061 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     3121 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     3181 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     3241 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     3301 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     3361 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     3421 gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     3481 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     3541 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga
     3601 tcgataagct tgatatcgaa ttcggagcac tgtcctccga acgtcggagc actgtcctcc
     3661 gaacgtcgga gcactgtcct ccgaacgtcg gagcactgtc ctccgaacgg agcatgtcct
     3721 ccgaacgtcg gagcactgtc ctccgaacga ctagttaggc gtgtacggtg ggaggcctat
     3781 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     3841 gacctccata gaagacaccg ggaccgatcc agcctctcga cattcgttgg atcatgagcg
     3901 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc
     3961 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
     4021 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
     4081 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
     4141 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
     4201 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
     4261 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
     4321 tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
     4381 agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac
     4441 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
     4501 aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
     4561 acctccctag caaactgggg cacaagctta attaagatcc ttgacttgcg gccgcaactc
     4621 ccacctgcaa catgcgtgac tgactgaggc cgcgactcta gagtcgacct gcatctaggc
     4681 gccggaatta gatctctcga ggttaacgaa ttctaccggg taggggaggc gcttttccca
     4741 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
     4801 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     4861 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
     4921 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
     4981 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
     5041 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
     5101 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     5161 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     5221 tgcagcccaa gcttaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     5281 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     5341 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     5401 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     5461 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     5521 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     5581 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     5641 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     5701 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     5761 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     5821 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     5881 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     5941 acaagtaaag cggccgcgac tctagagtcg acctgcaggc atgcaagctt gcaggcatgc
     6001 aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     6061 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6121 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6181 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6241 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6301 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6361 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     6421 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6481 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6541 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6601 gccgcctccc cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt
     6661 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6721 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     6781 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6841 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6901 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     6961 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     7021 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     7081 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     7141 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     7201 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     7261 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     7321 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     7381 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     7441 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     7501 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     7561 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     7621 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     7681 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     7741 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7801 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7861 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7921 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7981 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8041 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8101 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8161 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8221 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8281 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8341 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8401 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8461 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8521 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8581 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8641 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8701 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8761 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8821 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8881 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8941 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9001 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9061 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9121 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9181 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9241 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9301 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9361 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9421 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9481 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9541 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9601 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9661 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9721 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
     9781 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     9841 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9901 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9961 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
    10021 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
    10081 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
    10141 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
    10201 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
    10261 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
    10321 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
    10381 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
    10441 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
    10501 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
    10561 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
    10621 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
    10681 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
    10741 taatcttttc aacgcctggc actgc
//
