LOCUS       sequence-306310-        9107 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry fused to hyperactive ADAR catalytic domain, 
            control plasmid for HyperTRIBE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Biswas J, Rahman R, Gupta V, Rosbash M, Singer RH
  TITLE     MS2-TRIBE Evaluates Both Protein-RNA Interactions and Nuclear 
            Organization of Transcription by RNA Editing.
  JOURNAL   iScience. 2020 Jun 28;23(7):101318. doi: 10.1016/j.isci.2020.101318.
  PUBMED    32674054
REFERENCE   2  (bases 1 to 9107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-06-28"; volume: "23(7):101318. doi"; pages: " 
            10.1016/j.isci.2020.101318"
COMMENT     Sequence Label: pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP
FEATURES             Location/Qualifiers
     source          1..9107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      408..417
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             414..1121
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(559..577)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     827..846
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1140..1163
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2400..2441
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2469..2525
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2553..3266
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             2553..3263
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    3280..3868
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3333..3353)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3751..3762)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3943..4176
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4295..4313)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4381..4485
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4486..4554,4555..5346)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4704..4723)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5517..6105
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6006..6025
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6259..6276
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6516..6537
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6552..6557,6558..6575,6576..6582)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6590..6606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6595..6617
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6614..6630
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6614..6630
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6680..6699
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6891..7524
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7571..7696
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8193..8426
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8611..8655
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8948..9065
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggc ggccgccgcc accatggtga
      421 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
      481 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      541 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
      601 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
      661 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
      721 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
      781 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
      841 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
      901 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
      961 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     1021 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     1081 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtccggactc agatccatgg
     1141 actacaagga tgacgacgat aagggatcca tagactacag tccaatggtg gtgccacaga
     1201 agaacgtacc cctgccaatc gacgacaagt cgtcatcgat ggagttgcct cagatacacg
     1261 cggatacgat tggtcggttg gtcttagaaa agttcatgga agtaatcaag ggccaggagg
     1321 cttactcgcg tcgaaaggta ttagcgggca ttgtaatgac tgaaaacatg aatttttgtg
     1381 aagccaaagt tatttcagtt tcgacgggca ccaagtgtgt cagcggtgag catatgagtg
     1441 tgaacggagc tgtcctaaat gattcccatg ctgaaatagt ctccaggcgt tgtctcctca
     1501 aatatttata tgcacagctg gaccttcagt gcaatcaggc cacagcatat cagtcgattt
     1561 tcgtgaggaa tactgatggg caataccctt ataaactaaa atccggggta catttccatt
     1621 tgtatataaa tacagcacct tgtggggatg cacggatatt ttctcctcac gaaaacgaca
     1681 ctggtgttga taaacatcca aatagaaaag ctcgaggcca gctgcgcacc aaaatcgagt
     1741 ccggtcaggg gacgattcca gtgaaaagca gtgatggtat ccagacgtgg gatggcgtat
     1801 tgcagggcca acgcctgcta acaatgtcgt gctcggataa aattgcccgt tggaacatcg
     1861 tgggcatcca aggctcacta ttgtcttcca taattgaacc ggtgtacctg cattcgattg
     1921 tgctgggaag cctgttgcat ccggagcaca tgtaccgcgc agtttgcggc cgaattgaga
     1981 agtccattca aggcctgcca ccgccgtacc atctgaacaa gccgcgcctg gcactagtca
     2041 cttcggccga gccgcggaat caggccaagg ctcccaactt cgggatcaat tggaccattg
     2101 gggacaccga gctggaagtg gtgaactcgc taaccggtcg aaccattggc ggccaggtgt
     2161 ctcgcatcac aaagcaggcg tttttcgtta agtatggatt tctaatggca aacttgcccg
     2221 gtattttagt ccgcaaagta accactgact atgggcaaac caaagctaac gttaaggact
     2281 atcagatcgc aaagctagaa ttgttctctg cattcaagcg agaagacctt ggcagctggc
     2341 tgaagaaacc cattgaacaa gacgagttcg gtcttgccga atctagaggg cccttcgaag
     2401 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt aacggaccgg
     2461 gcagcggcgc caccaacttc agcctgctga agcaggccgg cgacgtggag gagaaccccg
     2521 gccccattgc atgcggttca ggttcatcag gctctaaagg tgaagaatta ttcactggtg
     2581 ttgtcccaat tttggttgaa ttagatggtg atgttaatgg tcacaaattt tctgtctccg
     2641 gtgaaggtga aggtgatgct acttacggta aattgacctt aaaatttatt tgtactactg
     2701 gtaaattgcc agttccatgg ccaaccttag tcactacttt aacttatggt gttcaatgtt
     2761 tttctagata cccagatcat atgaaacaac atgacttttt caagtctgcc atgccagaag
     2821 gttatgttca agaaagaact atttttttca aagatgacgg taactacaag accagagctg
     2881 aagtcaagtt tgaaggtgat accttagtta atagaatcga attaaaaggt attgatttta
     2941 aagaagatgg taacatttta ggtcacaaat tggaatacaa ctataactct cacaatgttt
     3001 acatcatggc tgacaaacaa aagaatggta tcaaagttaa cttcaaaatt agacacaaca
     3061 ttgaagatgg ttctgttcaa ttagctgacc attatcaaca aaatactcca attggtgatg
     3121 gtccagtctt gttaccagac aaccattact tatccactca atctgcctta tccaaagatc
     3181 caaacgaaaa gagagaccac atggtcttgt tagaatttgt tactgctgct ggtattaccc
     3241 atggtatgga tgaattgtac aaataaatcg atagatccta atcaacctct ggattacaaa
     3301 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3361 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3421 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3481 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3541 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3601 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3661 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     3721 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3781 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3841 cggatctccc tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg
     3901 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     3961 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     4021 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4081 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4141 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     4201 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta
     4261 aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     4321 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     4381 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4441 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4501 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4561 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4621 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4681 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     4741 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4801 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4861 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4921 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4981 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5041 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5101 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5161 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5221 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5281 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5341 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5401 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5461 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5521 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5581 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5641 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5701 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5761 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5821 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5881 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5941 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6001 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6061 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6121 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6181 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6241 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     6301 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa
     6361 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6421 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt
     6481 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     6541 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     6601 taacaatttc acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt
     6661 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6721 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
     6781 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
     6841 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct
     6901 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     6961 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     7021 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     7081 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     7141 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     7201 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     7261 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     7321 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7381 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7441 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7501 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     7561 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     7621 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     7681 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     7741 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     7801 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     7861 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     7921 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     7981 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg
     8041 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     8101 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     8161 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     8221 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     8281 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     8341 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     8401 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     8461 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     8521 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     8581 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     8641 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     8701 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     8761 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     8821 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     8881 tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg gcagtattca
     8941 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     9001 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     9061 attttcgggt ttattacagg gacagcagag atccagtttg gactagt
//
