LOCUS       sequence-306313-        9809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast integrating plasmid to express PYL1-VP64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Cunningham-Bryant D, Sun J, Fernandez B, Zalatan JG
  TITLE     CRISPR-Cas-Mediated Chemical Control of Transcriptional Dynamics in 
            Yeast.
  JOURNAL   Chembiochem. 2019 Jun 14;20(12):1519-1523. doi: 
            10.1002/cbic.201800823. Epub 2019 Apr 26.
  PUBMED    30710419
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/cbic.201800823"; journalName: "Chembiochem"; date: 
            "2019-06-14- 14"; volume: "20"; issue: "12"; pages: "1519-1523"
COMMENT     Sequence Label: pJS032
FEATURES             Location/Qualifiers
     source          1..9809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        323..1027
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1043..1063
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1649..1798
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     primer_bind     complement(3456..3475)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3457..3475)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3482..3499)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3482..3498)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3491..3513)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3639..4094
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3726..3745)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3936..3957
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4450..4469)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4569..4591
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4629..4647)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4732..4836
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4837..4905,4906..5697)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5055..5074)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5868..6456
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6357..6376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6610..6627
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6744..6765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6780..6785,6786..6803,6804..6810)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6818..6834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6823..6845
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6842..6858
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6842..6858
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        6879..6897
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     gene            7939..9295
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        7939..8282
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             8283..9092
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(8350..8369)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      9098..9295
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 tttgccggtg gtgcgaacaa tagagcgacc atgaccttga aggtgagacg cgcataaccg
       61 ctagagtact ttgaagagga aacagcaata gggttgctac cagtataaat agacaggtac
      121 atacaacact ggaaatggtt gtctgtttga gtacgctttc aattcatttg ggtgtgcact
      181 ttattatgtt acaatatgga agggaacttt acacttctcc tatgcacata tattaattaa
      241 agtccaatgc tagtagagaa ggggggtaac acccctccgc gctcttttcc gatttttttc
      301 taaaccgtgg aatatttcgg atatcctttt gttgtttccg ggtgtacaat atggacttcc
      361 tcttttctgg caaccaaacc catacatcgg gattcctata ataccttcgt tggtctccct
      421 aacatgtagg tggcggaggg gagatataca atagaacaga taccagacag gacataatgg
      481 gctaaacaag actacaccaa ttacactgcc tcattgatgg tggtacataa cgaactaata
      541 ctgtagccct agacttgata gccatcatca tatcgaagtt tcactaccct ttttccattt
      601 gccatctatt gaagtaataa taggcgcatg caacttcttt tctttttttt tcttttctct
      661 ctcccccgtt gttgtctcac catatccgca atgacaaaaa aatgatggaa gacactaaag
      721 gaaaaaatta acgacaaaga cagcaccaac agatgtcgtt gttccagagc tgatgagggg
      781 tatctcgaag cacacgaaac tttttccttc cttcattcac gcacactact ctctaatgag
      841 caacggtata cggccttcct tccagttact tgaatttgaa ataaaaaaaa gtttgctgtc
      901 ttgctatcaa gtataaatag acctgcaatt attaatcttt tgtttcctcg tcattgttct
      961 cgttcccttt cttccttgtt tctttttctg cacaatattt caagctatac caagcataca
     1021 atcaactatc tcatatacaa tgcccaaaaa gaaaagaaaa gtgatgggtg ggggcgcgcc
     1081 aactcaagac gaattcaccc aactctccca atcaatcgcc gagttccaca cgtaccaact
     1141 cggtaacggc cgttgctcat ctctcctagc tcagcgaatc cacgcgccgc cggaaacagt
     1201 atggtccgtg gtgagacgtt tcgataggcc acagatttac aaacacttca tcaaaagctg
     1261 taacgtgagt gaagatttcg agatgcgagt gggatgcacg cgcgacgtga acgtgataag
     1321 tggattaccg gcgaatacgt ctcgagagag attagatctg ttggacgatg atcggagagt
     1381 gactgggttt agtataaccg gtggtgaaca taggctgagg aattataaat cggttacgac
     1441 ggttcataga tttgagaaag aagaagaaga agaaaggatc tggaccgttg ttttggaatc
     1501 ttatgttgtt gatgtaccgg aaggtaattc ggaggaagat acgagattgt ttgctgatac
     1561 ggttattaga ttgaatcttc agaaacttgc ttcgatcact gaagctatga acgaattctc
     1621 aagcgctgca ggtactagtg gacgagccga tgctctcgac gattttgacc tcgatatgct
     1681 gggatccgac gctctcgatg acttcgacct tgacatgctg gggagtgacg ctctggacga
     1741 cttcgatctc gacatgctgg gctccgacgc cctggatgac ttcgatctgg acatgctcat
     1801 caactaagga tcctaggcgg ccgccaccgc ggtggagctc taagcaaata gctaaattat
     1861 atacgaatta atattatgat taagtgttta cgtgagtgcg atatttttat tactatctta
     1921 tacagttgta tatactctat aaaatgagtt gtctattaat taacgcgatg aatgctttct
     1981 gggtttacct ctccaacaac tctagtttac ttctcaatac attcaattgt atttgatttg
     2041 tcaatacttc atcattaatc aattctatag ttttgttttt ctcgtttatt tccaaattta
     2101 atgcatcaat tttattattc aatttgtcgt tgattttggt taatgatttt atggtttgat
     2161 ctctggcatt gattgtttgt gttagttttt cattattgat aattaaatta tttaagttag
     2221 ttatcaactc ggtgttttca agtttcaagt tttcaatttc tttagagttt attagatttg
     2281 tcaaagtttc tgaattgctt gattggtcct gtagaagagt atttgttgtt gtggataatt
     2341 gattcaattt ttgagacaat tgctggaagg cgttgaaata tctagcatca atctcatggt
     2401 ttttttcccg agagtctcgt agattcaatt gttttaatat atcttgggac cactcttgat
     2461 ttgaactcat ggaaattaaa ctgggtgttg tgttgtggtg taatgattgt accccctttg
     2521 cttataattg tgtggccatt tcacgtgcat caagtccgta ctatcttcac cactataaac
     2581 tcttataggt ggattattcg tttcgaatgc tggtggcagt gccggtttga aaataatctt
     2641 acctacccat gggtggtctt ccgttaacgg tttgatgcat cttaatgagt ctttgatata
     2701 tcttttcctt gaaactagtt gtgtcgccgg tgcccacttc gaatcgttac tggattgatt
     2761 cttatttcca cattttagtg acaatttctt tggaaaatca tgatttaaac aaccagtcac
     2821 ggaaaagctt ttcgtactga atattgtacc gtactcaacg tccgcattct catagcgaga
     2881 ttctctttca tccaaatata ccaatctatt gatgtcatca tcctcaacat atccatcttc
     2941 atcatcctct tcgtaattag catcgtcttc gtcttcgtca tcatcttcat ctccatttac
     3001 atcttcattg ccacggttta acttttcttc acgattcgta cgctcagcag aattcgaaat
     3061 agttgacctt cctgttccct cgtataccgg ttcaaaatta ttaattttca acactgaccg
     3121 atcacagcgc aaaggatcta tagggttgga attcgatcta cttctgaagt tttcatggat
     3181 ggatgtttgt gccgttaaaa cagaatgtgc tgtgggagaa aaagtggaag atagaacaga
     3241 agtattggta ttagtagtac taccattgtc atcgatttct tcttgaacac tattcagttc
     3301 cttcgatctc attttttgtc caatgtccat attagagttt tttctcgaac tggagtctgt
     3361 tttaaagaaa tttcttagtt ttgatgtaaa taagccacaa aaatcagttt gagaactctc
     3421 ccctgccagc tagcgtttaa acggccacgc tggcccccta tagtgagtcg tattacaatt
     3481 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     3541 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     3601 gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     3661 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     3721 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     3781 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3841 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3901 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3961 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4021 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4081 aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4141 cgcatagggt aataactgat ataattaaat tgaagctcta atttgtgagt ttagtattag
     4201 gatctctacc cttggcgaaa agtcctctgc caacaatgat gatatctgat ccaccactta
     4261 caacttcgtc gacggttctg tactgctgac ccaatatgcg tatatatacc aatctaagtc
     4321 tgtgctcctt ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaag
     4381 gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga attgaaaagg tggtatggtg
     4441 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4501 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4561 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4621 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4681 ttagacgtcg gcgcgccgag gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4741 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4801 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4861 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4921 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4981 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5041 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5101 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5161 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5221 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5281 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5341 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5401 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5461 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5521 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5581 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5641 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5701 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5761 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5821 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5881 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5941 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6001 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6061 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6121 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6181 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6241 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6301 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6361 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6421 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6481 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6541 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6601 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6661 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     6721 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     6781 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     6841 acaggaaaca gctatgacca tgattacgcc aagctcggaa ttaaccctca ctaaagggaa
     6901 caaaagctgg gccggccatt taaatgttta aaccaaaatc aaatcacggt atggcatttc
     6961 tttacatatt ctcaatacct gactgacata gtatgcgcta catagttttg tcctattttt
     7021 tttacaccgc gatttgaagt ctaaagccag agaatgagga aaaacgaaac aaactgagat
     7081 catttgtata aggggagtca ctgtacagct gccaatacca attttccttc tgtcaaatca
     7141 aaaaagtatc cgcatacaca tacacccctc cctctacaat cttaattatt tactgattcc
     7201 tttcgtattt gtccagtatg agcaatacta agcataccac ttctcatcac atggaactca
     7261 aaaggatcat tatccttaca cttttattca tactcataat gttgatattt cgaaactcag
     7321 tgtccttcaa aatgacattt caagaactac tgccacgatt ttacaaaaag aattcaaact
     7381 cagttagtaa taataacagg ccctcatcta ttttctcgga aaacttggtg gattttgatg
     7441 atgttaacat ggtcgataag accagactgt ttattttttt atttttcagt ttcatcatta
     7501 ctataccgtt tatggtataa attagaaaag ttaaagcagc atgttttcat ttgaaacaaa
     7561 tactaatgca ataataggta caccatctac tacataatta attgatagtt tccttttccg
     7621 ttaagtgcat gcataggacg cccataattt tttaagttaa aaaagcatgt atttacctat
     7681 tcgggaaatt tacatgacat ggatgccata aggaacgaaa atgaaacatg catgtcagag
     7741 gaaaaagaac aaagcgagag gaaaaagctg ttgcattacc acgtaatttt gtatataaat
     7801 atctgataaa taaccatttt atttccatcc acttcttctg tcgttcatcc gttcattgac
     7861 atcactagag acaccagcga gctatcatct gccagatctg tttagcttgc cttgtccccg
     7921 ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg
     7981 cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta
     8041 taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc
     8101 tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac
     8161 gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat
     8221 atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa
     8281 ccatgggtaa ggaaaagact cacgtttcga ggccgcgatt aaattccaac atggatgctg
     8341 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     8401 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     8461 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
     8521 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     8581 ccggcaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     8641 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     8701 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     8761 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     8821 tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     8881 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     8941 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     9001 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     9061 agtttcattt gatgctcgat gagtttttct aatcagtact gacaataaaa agattcttgt
     9121 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa
     9181 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag
     9241 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc
     9301 gattcgatac taacgccgcc atccagtgtc gaaaacggta ccgggcccta aaacaagaag
     9361 agggttgact acatcacgat gagggggatc gaagaaatga tggtaaatga aataggaaat
     9421 caaggagcat gaaggcaaaa gacaaatata agggtcgaac gaaaaataaa gtgaaaagtg
     9481 ttgatatgat gtatttggct ttgcggcgcc gaaaaaacga gtttacgcaa ttgcacaatc
     9541 atgctgactc tgtggcggac ccgcgctctt gccggcccgg cgataacgct gggcgtgagg
     9601 ctgtgcccgg cggagttttt tgcgcctgca ttttccaagg tttaccctgc gctaaggggc
     9661 gagattggag aagcaataag aatgccggtt ggggttgcga tgatgacgac cacgacaact
     9721 ggtgtcatta tttaagttgc cgaaagaacc tgagtgcatt tgcaacatga gtatactaga
     9781 agaatgagcc aagacttgcg agacgcgag
//
