LOCUS       sequence-30632-e         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  TITLE     HA-PKB
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HA-PKB-pcDNA3-K179A
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             158..184
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             164..190
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt
      121 gctggaattc tgcagataat tcggctagac caccatgtac ccatacgatg ttccagatta
      181 cgcaagcttg ggtggtccca tggacgacgt ggccatcgtg aaggagggct ggctgcacaa
      241 gcgaggtgag tacatcaaga cgtggcggcc gcgttacttc ctcctgaaga acgatggcac
      301 gttcattggc tacaaggagc ggccgcagga cctggagcag cgtgagtcgc ccctcaacaa
      361 cttctccgtg gcccaatgcc agctgatgaa gacggagcgg ccgcggccca acaccttcat
      421 catccgctgc ctgcagtgga ccacggtcat cgagcgcacg ttccacgtgg agacgcccga
      481 ggagcgggag gagtggacca ccgccatcca gacggtggcc gacgggctca agaggcagga
      541 ggaggagacg atggacttcc ggtcgggctc acccggcgag aactcggggg ccgaggagat
      601 ggaggtgtcg ctggccaagc ccaagcaccg cgtgaccatg
//
