LOCUS       sequence-306324-        5790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Triggers HilA overexpression from Salmonella upon induction with 
            arabinose without any synthetic protein expressed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Chabloz A, Schaefer JV, Kozieradzki I, Cronin SJF, Strebinger D, 
            Macaluso F, Wald J, Rabbitts TH, Pluckthun A, Marlovits TC, 
            Penninger JM
  TITLE     Salmonella-based platform for efficient delivery of functional 
            binding proteins to the cytosol.
  JOURNAL   Commun Biol. 2020 Jul 3;3(1):342. doi: 10.1038/s42003-020-1072-4.
  PUBMED    32620833
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-07-3"; volume: "3(1):342. doi"; pages: " 
            10.1038/s42003-020-1072-4"
COMMENT     Sequence Label: pCASP-HilA empty vector
FEATURES             Location/Qualifiers
     source          1..5790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      17..103
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(211..1089)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1116..1400
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1323..1342
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_feature    3644..3702
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3735..3752)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3905..3976
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(4133..4721)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4213..4232)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4809..4903)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4924..5583)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5498..5517
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5584..5686)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     5752..5770
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggccgctgat tggcatcaaa taaaacgaaa ggctcagtcg aaagactggg actttcgttt
       61 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagaccga
      121 tgcataatgt gcctgtcaaa tggacgaagc agggattctg caaaccctat gctactccgt
      181 caagccgtca attgtctgat tcgttaccaa ttatgacaac ttgacggcta catcattcac
      241 tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac
      301 ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat
      361 ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa
      421 gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac
      481 atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg
      541 acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat
      601 gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc
      661 cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc
      721 atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca
      781 gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg
      841 accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa
      901 ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc
      961 tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt
     1021 taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc
     1081 ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat
     1141 cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc
     1201 gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa
     1261 aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg
     1321 ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca
     1381 actctctact gtttctccat acccgttttt ttgggctagc gaattcgagc tcggtacccc
     1441 cgagcccgta gaatatgaca ttaagctcat aataaaagct caacctgacc gttagtacta
     1501 acagcagaat tactgaaaca gtagattcta tcctaacgac ttgtattagt tattataact
     1561 tttcaccctg taagagaata cactattatc atgccacatt ttaatcctgt tcctgtatcg
     1621 aataaaaaat tcgtctttga tgatttcata ctcaacatgg acggctccct gctacgctca
     1681 gaaaagaaag tcaatattcc gccaaaagaa tatgccgttc tggtcatcct gctcgaagcc
     1741 gccggcgaga ttgtgagtaa aaacacctta ctggaccagg tatggggcga cgcggaagtt
     1801 aacgaagaat ctcttacccg ctgtatttat gccttacgac gtattctgtc ggaagataaa
     1861 gagcatcgtt acattgaaac actgtacgga cagggctatc ggtttaatcg tccggtcgta
     1921 gtggtgtctc cgccagcgcc gcaacctacg actcatacat tggcgatact tccttttcag
     1981 atgcaggatc aggttcaatc cgagagtctg cattactcta tcgtgaaggg attatcgcag
     2041 tatgcgccct ttggcctgag cgtgctgccg gtgaccatta cgaagaactg ccgcagtgtt
     2101 aaggatattc ttgagctcat ggatcaatta cgccccgatt attatatctc cgggcagatg
     2161 atacccgatg gtaatgataa tattgtacag attgagatag ttcgggttaa aggttatcac
     2221 ctgctgcacc aggaaagcat taagttgata gaacaccaac ccgcttctct cttgcaaaac
     2281 aaaattgcga atcttttgct cagatgtatt cccggacttc gctgggacac aaagcagatt
     2341 agcgagctaa attcgattga cagtactatg gtttacttac gcggtaagca tgagttaaat
     2401 caatacaccc cctatagctt acagcaagcg cttaaattgc tgactcaatg cgttaacatg
     2461 tcgccaaaca gcattgcgcc ttactgtgcg ctggcagaat gctacctcag catggcgcaa
     2521 atggggattt ttgataaaca aaacgctatg atcaaagcta aagaacatgc gattaaggcg
     2581 acagagctgg accacaataa tccacaagct ttaggattac tggggctaat taatacgatt
     2641 cactcagaat acatcgtcgg gagtttgcta ttcaaacaag ctaacttact ttcgcccatt
     2701 tctgcagata ttaaatatta ttatggctgg aatcttttca tggctggtca gttggaggag
     2761 gccttacaaa cgattaacga gtgtttaaaa ttggacccaa cgcgcgcagc cgcagggatc
     2821 actaagctgt ggattaccta ttatcatacc ggtattgatg atgctatacg tttaggcgat
     2881 gaattacgct cacaacacct gcaggataat ccaatattat taagtatgca ggttatgttt
     2941 ctttcgctta aaggtaaaca tgaactggca cgaaaattaa ctaaagaaat atccacgcag
     3001 gaaataacag gacttattgc tgttaatctt ctttacgctg aatattgtca gaatagtgag
     3061 cgtgccttac cgacgataag agaatttctg gaaagtgaac agcgtataga taataatccg
     3121 ggattattac cgttagtgct ggttgcccac ggcgaagcta ttgccgagaa aatgtggaat
     3181 aaatttaaaa acgaagacaa tatttggttc aaaagatgga aacaggatcc ccgcttgatt
     3241 aaattacggt aaaatctgag agaggagata tgcattattt ttttatcatc gtaatctggt
     3301 tgcttagcat aaatacggca tgggctgatt gctggcttca ggctgaaaaa atgttcaata
     3361 ttgaatccga actactttac gctatcgccc agcaggaatc ggcgatgaaa cctggcgcca
     3421 ttggtcataa ccgagatggt tcaaccgatc ttggcctgat gcaaattaac agcttccata
     3481 tgaaaaggct gaaaaaaatg gggattagtg aaaaacagtt gttacaggac ccctgcattt
     3541 ctgtcattgt gggcgcctcc attttatcag atatgatgaa aatctacggt tatagctggg
     3601 aggccgttgg cgcttataat gccgggacgt cgccgaaacg atcgaattcg agctcggtac
     3661 ccggggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga
     3721 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
     3781 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
     3841 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
     3901 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     3961 gtcggtgaac gctctcctga gtctagggcg ttcggctgcg gcgagcggta tcagctcact
     4021 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4081 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4141 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4201 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4261 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4321 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4381 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4441 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4501 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4561 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4621 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     4681 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4741 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgacta
     4801 gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     4861 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
     4921 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
     4981 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
     5041 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
     5101 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
     5161 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
     5221 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
     5281 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     5341 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
     5401 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     5461 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     5521 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     5581 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     5641 tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg
     5701 ccagatatcg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     5761 atcacgaggc cctttcgtct tcactaaagc
//
