LOCUS       sequence-306329-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses synonymized MS2 coat protein dimer (MCP) fused to 
            hyperactive ADAR catalytic domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Biswas J, Rahman R, Gupta V, Rosbash M, Singer RH
  TITLE     MS2-TRIBE Evaluates Both Protein-RNA Interactions and Nuclear 
            Organization of Transcription by RNA Editing.
  JOURNAL   iScience. 2020 Jun 28;23(7):101318. doi: 10.1016/j.isci.2020.101318.
  PUBMED    32674054
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-06-28"; volume: "23(7):101318. doi"; pages: " 
            10.1016/j.isci.2020.101318"
COMMENT     Sequence Label: pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      407..416
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             419..439
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             443..469
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             443..469
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             470..493
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2462..2503
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2531..2587
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2615..3328
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             2615..3325
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    3342..3930
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3395..3415)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3813..3824)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4005..4238
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4357..4375)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4443..4547
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4548..4616,4617..5408)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4766..4785)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5579..6167
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6068..6087
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6321..6338
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6578..6599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6614..6619,6620..6637,6638..6644)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6652..6668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6657..6679
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6676..6692
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6676..6692
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6742..6761
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6953..7586
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7633..7758
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8255..8488
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8673..8717
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9010..9127
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggc ggccgcgcca ccatgggccc
      421 aaaaaagaaa agaaaagttg gctaccccta cgacgtgccc gactacgccc tggaagttct
      481 gttccagggg cccctgttat taattaacgc ttctaacttt actcagttcg ttctcgtcga
      541 caatggcgga actggcgacg tgactgtcgc cccaagcaac ttcgctaacg ggatcgctga
      601 atggatcagc tctaactcgc gttcacaggc ttacaaagta acctgtagcg ttcgtcagag
      661 ctctgcgcag aatcgcaaat acaccatcaa agtcgaggtg cctaaaggcg cctggcgttc
      721 gtacttaaat atggaactaa ccattccaat tttcgccacg aattccgact gcgagcttat
      781 tgttaaggca atgcaaggtc tcctaaaaga tggaaacccg attccctcag caatcgcagc
      841 aaactccggc atctacgcca tggccagcaa cttcacccag ttcgtgctgg tggacaacgg
      901 cggcaccggc gacgtgaccg tggcccccag caacttcgcc aacggcatcg ccgagtggat
      961 cagcagcaac agcagaagcc aggcctacaa ggtgacctgc agcgtgagac agagcagcgc
     1021 ccagaacaga aagtacacca tcaaggtgga ggtgcccaag ggcgcctgga gaagctacct
     1081 gaacatggag ctgaccatcc ccatcttcgc caccaacagc gactgcgagc tgatcgtgaa
     1141 ggccatgcag ggcctgctga aggacggcaa ccccatcccc agcgccatcg ccgccaacag
     1201 cggcatctac gccgacccac cgggaggtac catggactac agtccaatgg tggtgccaca
     1261 gaagaacgta cccctgccaa tcgacgacaa gtcgtcatcg atggagttgc ctcagataca
     1321 cgcggatacg attggtcggt tggtcttaga aaagttcatg gaagtaatca agggccagga
     1381 ggcttactcg cgtcgaaagg tattagcggg cattgtaatg actgaaaaca tgaatttttg
     1441 tgaagccaaa gttatttcag tttcgacggg caccaagtgt gtcagcggtg agcatatgag
     1501 tgtgaacgga gctgtcctaa atgattccca tgctgaaata gtctccaggc gttgtctcct
     1561 caaatattta tatgcacagc tggaccttca gtgcaatcag gccacagcat atcagtcgat
     1621 tttcgtgagg aatactgatg ggcaataccc ttataaacta aaatccgggg tacatttcca
     1681 tttgtatata aatacagcac cttgtgggga tgcacggata ttttctcctc acgaaaacga
     1741 cactggtgtt gataaacatc caaatagaaa agctcgaggc cagctgcgca ccaaaatcga
     1801 gtccggtcag gggacgattc cagtgaaaag cagtgatggt atccagacgt gggatggcgt
     1861 attgcagggc caacgcctgc taacaatgtc gtgctcggat aaaattgccc gttggaacat
     1921 cgtgggcatc caaggctcac tattgtcttc cataattgaa ccggtgtacc tgcattcgat
     1981 tgtgctggga agcctgttgc atccggagca catgtaccgc gcagtttgcg gccgaattga
     2041 gaagtccatt caaggcctgc caccgccgta ccatctgaac aagccgcgcc tggcactagt
     2101 cacttcggcc gagccgcgga atcaggccaa ggctcccaac ttcgggatca attggaccat
     2161 tggggacacc gagctggaag tggtgaactc gctaaccggt cgaaccattg gcggccaggt
     2221 gtctcgcatc acaaagcagg cgtttttcgt taagtatgga tttctaatgg caaacttgcc
     2281 cggtatttta gtccgcaaag taaccactga ctatgggcaa accaaagcta acgttaagga
     2341 ctatcagatc gcaaagctag aattgttctc tgcattcaag cgagaagacc ttggcagctg
     2401 gctgaagaaa cccattgaac aagacgagtt cggtcttgcc gaatctagag ggcccttcga
     2461 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtaacggacc
     2521 gggcagcggc gccaccaact tcagcctgct gaagcaggcc ggcgacgtgg aggagaaccc
     2581 cggccccatt gcatgcggtt caggttcatc aggctctaaa ggtgaagaat tattcactgg
     2641 tgttgtccca attttggttg aattagatgg tgatgttaat ggtcacaaat tttctgtctc
     2701 cggtgaaggt gaaggtgatg ctacttacgg taaattgacc ttaaaattta tttgtactac
     2761 tggtaaattg ccagttccat ggccaacctt agtcactact ttaacttatg gtgttcaatg
     2821 tttttctaga tacccagatc atatgaaaca acatgacttt ttcaagtctg ccatgccaga
     2881 aggttatgtt caagaaagaa ctattttttt caaagatgac ggtaactaca agaccagagc
     2941 tgaagtcaag tttgaaggtg ataccttagt taatagaatc gaattaaaag gtattgattt
     3001 taaagaagat ggtaacattt taggtcacaa attggaatac aactataact ctcacaatgt
     3061 ttacatcatg gctgacaaac aaaagaatgg tatcaaagtt aacttcaaaa ttagacacaa
     3121 cattgaagat ggttctgttc aattagctga ccattatcaa caaaatactc caattggtga
     3181 tggtccagtc ttgttaccag acaaccatta cttatccact caatctgcct tatccaaaga
     3241 tccaaacgaa aagagagacc acatggtctt gttagaattt gttactgctg ctggtattac
     3301 ccatggtatg gatgaattgt acaaataaat cgatagatcc taatcaacct ctggattaca
     3361 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     3421 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     3481 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     3541 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     3601 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     3661 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     3721 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     3781 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     3841 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     3901 gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa
     3961 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4021 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     4081 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     4141 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     4201 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     4261 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat
     4321 taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     4381 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4441 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4501 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4561 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4621 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4681 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4741 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     4801 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4861 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4921 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4981 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5041 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5101 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5161 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5221 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5281 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5341 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5401 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5461 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5521 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5581 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5641 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5701 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5761 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5821 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5881 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5941 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6001 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6061 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6121 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6181 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6241 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6301 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     6361 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact
     6421 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6481 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt
     6541 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     6601 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     6661 gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat
     6721 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6781 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
     6841 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
     6901 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
     6961 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     7021 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     7081 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     7141 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     7201 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     7261 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     7321 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     7381 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     7441 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7501 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7561 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     7621 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     7681 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     7741 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     7801 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     7861 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     7921 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     7981 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     8041 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     8101 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     8161 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     8221 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     8281 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     8341 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     8401 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     8461 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     8521 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     8581 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     8641 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     8701 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     8761 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     8821 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     8881 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     8941 gatccattcg attagtgaac ggatctcgac ggtatcgccg aattcacaaa tggcagtatt
     9001 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     9061 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     9121 aaattttcgg gtttattaca gggacagcag agatccagtt tggactagt
//
