LOCUS       sequence-306330-       10829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid for integration and constitutive 
            expression of mCherry fused to oncogenic G12V-KRAS in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Chabloz A, Schaefer JV, Kozieradzki I, Cronin SJF, Strebinger D, 
            Macaluso F, Wald J, Rabbitts TH, Pluckthun A, Marlovits TC, 
            Penninger JM
  TITLE     Salmonella-based platform for efficient delivery of functional 
            binding proteins to the cytosol.
  JOURNAL   Commun Biol. 2020 Jul 3;3(1):342. doi: 10.1038/s42003-020-1072-4.
  PUBMED    32620833
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-07-3"; volume: "3(1):342. doi"; pages: " 
            10.1038/s42003-020-1072-4"
COMMENT     Sequence Label: Lenti-mCherry-G12V-KRAS-IRES-Blast
FEATURES             Location/Qualifiers
     source          1..10829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(57..76)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        248..627
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        629..827
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     782..802
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             845..1025
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1072..1197
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1690..1923
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2108..2152
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2450..2567
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2660..2871
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2875..2884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2881..3588
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3026..3044)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3294..3313
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(3604..3636,3637..3639,3640..4170)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     intron          4222..4451
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4434..4453
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4509..5080
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4674..4691)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4901..4920
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5113..5511
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5531..6119
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5584..5604)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6002..6013)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6122..6138)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6123..6139)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6644..6824
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6850..6867)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6856..7080
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7126..7554
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7213..7232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7423..7444
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7563..7583)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7568..7897
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7748..7883
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7810..7829
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7945..7992
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8011..8385
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8515..8636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8552..8571)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8606..8625
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8685..8701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8685..8701)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8698..8720)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8709..8725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8733..8739,8740..8757,8758..8763))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8778..8799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8916..8933)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9087..9675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9167..9186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9846..10637,10638..10706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10469..10488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10707..10811)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
       61 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
      121 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
      181 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
      241 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      301 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      361 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      421 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      481 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      541 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      601 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      661 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      721 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      781 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg
      841 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      901 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      961 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     1021 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     1081 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     1141 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     1201 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     1261 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     1321 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     1381 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     1441 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     1501 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     1561 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     1621 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1681 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1741 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1801 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1861 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1921 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1981 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     2041 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     2101 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     2161 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     2221 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     2281 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     2341 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     2401 ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa
     2461 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2521 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     2581 gggacagcag agatccagtt tggttaatta gctagctagg tcttgaaagg agtgggaatt
     2641 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     2701 ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     2761 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     2821 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaggccacc
     2881 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     2941 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     3001 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     3061 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     3121 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     3181 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     3241 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     3301 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     3361 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     3421 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     3481 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     3541 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga
     3601 tctatgactg aatataaact tgtggtagtt ggagctgttg gcgtaggcaa gagtgccttg
     3661 acgatacagc taattcagaa tcattttgtg gacgaatatg atccaacaat agaggattcc
     3721 tacaggaagc aagtagtaat tgatggagaa acctgtctct tggatattct cgacacagca
     3781 ggtcaagagg agtacagtgc aatgagggac cagtacatga ggactgggga gggctttctt
     3841 tgtgtatttg ccataaataa tactaaatca tttgaagata ttcaccatta tagagaacaa
     3901 attaaaagag ttaaggactc tgaagatgta cctatggtcc tagtaggaaa taaatgtgat
     3961 ttgccttcta gaacagtaga cacaaaacag gctcaggact tagcaagaag ttatggaatt
     4021 ccttttattg aaacatcagc aaagacaaga cagggtgttg atgatgcctt ctatacatta
     4081 gttcgagaaa ttcgaaaaca taaagaaaag atgagcaaag atggtaaaaa gaagaaaaag
     4141 aagtcaaaga caaagtgtgt aattatgtaa cgatccagtg tgctggaatt aattcgctgt
     4201 ctgcgagggc cagctgttgg ggtgagtact ccctctcaaa agcgggcatg acttctgcgc
     4261 taagattgtc agtttccaaa aacgaggagg atttgatatt cacctggccc gcggtgatgc
     4321 ctttgagggt ggccgcgtcc atctggtcag aaaagacaat ctttttgttg tcaagcttga
     4381 ggtgtggcag gcttgagatc tggccataca cttgagtgac aatgacatcc actttgcctt
     4441 tctctccaca ggtgtccact cccaggtcca actgcaggtc gagcatgcat ctagggcggc
     4501 caattccgcc cctctccccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     4561 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     4621 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     4681 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     4741 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     4801 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     4861 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     4921 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     4981 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     5041 ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccaca acccacaagg
     5101 agacgacctt ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca
     5161 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc
     5221 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt
     5281 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc
     5341 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg
     5401 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg
     5461 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta acgatatcaa
     5521 gcttatcggt aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5581 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5641 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5701 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5761 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5821 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5881 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     5941 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6001 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6061 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     6121 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     6181 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     6241 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6301 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     6361 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     6421 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     6481 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     6541 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     6601 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     6661 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6721 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6781 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     6841 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     6901 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6961 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     7021 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     7081 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     7141 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7201 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7261 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7321 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7381 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7441 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7501 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     7561 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7621 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     7681 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     7741 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7801 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     7861 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     7921 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     7981 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     8041 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     8101 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     8161 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     8221 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     8281 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     8341 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     8401 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     8461 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     8521 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8581 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8641 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     8701 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8761 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8821 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8881 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8941 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9001 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9061 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9121 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9181 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9241 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9301 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9361 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9421 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9481 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9541 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9601 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9661 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9721 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9781 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9841 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9901 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9961 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10021 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    10081 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    10141 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    10201 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    10261 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10321 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10381 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10441 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10501 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10561 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10621 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10681 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10741 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10801 ggggttccgc gcacatttcc ccgaaaagt
//
