LOCUS       sequence-306335-       10724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant mitoTALEN vector assembly, Destination vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Kazama T, Okuno M, Watari Y, Yanase S, Koizuka C, Tsuruta Y, Sugaya 
            H, Toyoda A, Itoh T, Tsutsumi N, Toriyama K, Koizuka N, Arimura SI
  TITLE     Curing cytoplasmic male sterility via TALEN-mediated mitochondrial 
            genome editing.
  JOURNAL   Nat Plants. 2019 Jul;5(7):722-730. doi: 10.1038/s41477-019-0459-z. 
            Epub 2019 Jul 8.
  PUBMED    31285556
REFERENCE   2  (bases 1 to 10724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-019-0459-z"; journalName: "Nat Plants"; date: 
            "2019-07"; volume: "5"; issue: "7"; pages: "722-730"
COMMENT     Sequence Label: pDESTpK7WG2mito
FEATURES             Location/Qualifiers
     source          1..10724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        116..295
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             329..1123
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(383..402)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     993..1012
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1163..1415
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1508..1532
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2060..2851
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3095..3683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3584..3603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3837..3854
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3869..4009
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3935..3954)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4095..4117
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4353..4547
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4613..5680)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(6114..6743)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8043..8067
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        8151..8496
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     8439..8459
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    8516..8571
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    8768..8892
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(8917..8922,8923..8940,8941..8947)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             9001..9660
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9067..9086)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             10002..10307
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     10186..10205
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(10348..10472)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggccctctag aggatccccg ggtaccctcg aattatcata catgagaatt aagggagtca
       61 cgttatgacc cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacagaacc
      121 gcaacgttga aggagccact cagccgcggg tttctggagt ttaatgagct aagcacatac
      181 gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc tagcaaatat
      241 ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc
      301 tcatattcac tctcaactcg atcgaggcat gattgaacaa gatggattgc acgcaggttc
      361 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
      421 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
      481 cgacctgtcc ggtgccctga atgaactcca agacgaggca gcgcggctat cgtggctggc
      541 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
      601 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
      661 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
      721 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
      781 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
      841 cgccaggctc aaggcgcgga tgcccgacgg cgaggatctc gtcgtgaccc acggcgatgc
      901 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
      961 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     1021 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     1081 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     1141 ggactctagc tagagtcaag cagatcgttc aaacatttgg caataaagtt tcttaagatt
     1201 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     1261 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     1321 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     1381 attatcgcgc gcggtgtcat ctatgttact agatcgaccg gcatgcaagc tgataattca
     1441 attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
     1501 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
     1561 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
     1621 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
     1681 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
     1741 acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc
     1801 tcgttcggtt cgtaagctgt aatgcaagta gcgtaactgc cgtcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttcttgttat
     1921 gacatgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     1981 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     2041 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     2101 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     2161 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     2221 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     2281 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     2341 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     2401 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     2461 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     2521 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     2581 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     2641 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     2701 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     2761 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     2821 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     2881 gccgcttcgc cggcgttaac tcaagcgatt agatgcacta agcacataat tgctcacagc
     2941 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca
     3001 aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3061 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3121 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3181 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3241 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3301 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3361 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3421 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3481 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3541 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3601 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3661 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3721 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3781 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3841 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     3901 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     3961 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     4021 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4081 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4141 cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc
     4201 cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga
     4261 taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt
     4321 gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag
     4381 ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag
     4441 tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc
     4501 ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt
     4561 ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac
     4621 cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct
     4681 gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca
     4741 cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga
     4801 acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc
     4861 cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc
     4921 cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc
     4981 cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca
     5041 ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc
     5101 gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact
     5161 tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc
     5221 ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc
     5281 acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt
     5341 agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca
     5401 tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt
     5461 gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg
     5521 attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct
     5581 cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca
     5641 gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gtcacgccga ttcctcgggc
     5701 ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa
     5761 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
     5821 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
     5881 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
     5941 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
     6001 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
     6061 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
     6121 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
     6181 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
     6241 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
     6301 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
     6361 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
     6421 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
     6481 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
     6541 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
     6601 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
     6661 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
     6721 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
     6781 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
     6841 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
     6901 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
     6961 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
     7021 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
     7081 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
     7141 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
     7201 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
     7261 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
     7321 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
     7381 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
     7441 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
     7501 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
     7561 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
     7621 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt
     7681 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct
     7741 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca
     7801 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc
     7861 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat
     7921 caaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac
     7981 ggataaacct tttcacgccc ttttaaatat ccgttattct aataaacgct cttttctctt
     8041 aggtttaccc gccaatatat cctgtcaaac actgatagtt taaactgaag gcgggaaacg
     8101 acaatctgat ccaagctcaa gctaagctcc tcagaagacc agagggctat tgagactttt
     8161 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     8221 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
     8281 aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc cccacccacg
     8341 aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
     8401 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     8461 tctatataag gaagttcatt tcatttggag aggacaggct tcttgagatc cttcaacaat
     8521 taccaacaac aacaaacaac aaacaacatt acaattacta tttacaatta cagtcgaccc
     8581 acgcgtccga taatttcccc aatttctcag agagactcaa aatcaccacc accaccaaat
     8641 cgccgccatg tttaaacaag cttctcgtct cctctcccga tctgtcgccg ccgcatcttc
     8701 caaatcggtg acgactcgtg ccttttcaac ggaacttcca tcgacgctcg attccgctag
     8761 tgatatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
     8821 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
     8881 ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg
     8941 tataatgtgt ggattttgag ttaggatccg gcgagatttt caggagctaa ggaagctaaa
     9001 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
     9061 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
     9121 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
     9181 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt
     9241 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
     9301 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
     9361 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     9421 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     9481 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
     9541 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat
     9601 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
     9661 acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt
     9721 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtgtgcta
     9781 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata
     9841 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg
     9901 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga
     9961 aatgaacggc tcttttgctg acgagaacag ggactggtga aatgcagttt aaggtttaca
    10021 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca
    10081 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct
    10141 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg
    10201 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg
    10261 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc
    10321 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat
    10381 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
    10441 gtttctcgtt cagctttctt gtacaaagtg gtgatatccc gcggccatgc tagagtccgc
    10501 aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg
    10561 tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca
    10621 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
    10681 attcctaaaa ccaaaatcca gtgacctgca ggcatgcgac gtcg
//
