LOCUS       sequence-306346-       16979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant mitoTALEN vector to cut Brassica CMS gene orf125 (orf138).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16979)
  AUTHORS   Kazama T, Okuno M, Watari Y, Yanase S, Koizuka C, Tsuruta Y, Sugaya 
            H, Toyoda A, Itoh T, Tsutsumi N, Toriyama K, Koizuka N, Arimura SI
  TITLE     Curing cytoplasmic male sterility via TALEN-mediated mitochondrial 
            genome editing.
  JOURNAL   Nat Plants. 2019 Jul;5(7):722-730. doi: 10.1038/s41477-019-0459-z. 
            Epub 2019 Jul 8.
  PUBMED    31285556
REFERENCE   2  (bases 1 to 16979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-019-0459-z"; journalName: "Nat Plants"; date: 
            "2019-07"; volume: "5"; issue: "7"; pages: "722-730"
COMMENT     Sequence Label: pK7WG2-mitoTAL1-orf125
FEATURES             Location/Qualifiers
     source          1..16979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        116..295
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             329..1123
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(383..402)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     993..1012
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1163..1415
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1508..1532
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2060..2851
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3095..3683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3584..3603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3837..3854
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3869..4009
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3935..3954)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4095..4117
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4353..4547
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4613..5680)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(6114..6743)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8043..8067
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        8151..8496
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    8516..8571
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    8768..8792
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8795..8821
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             11372..11959
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     protein_bind    complement(11997..12017)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      12022..12270
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        12855..13200
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    13220..13275
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    13461..13481
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             13484..13528
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             16082..16669
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     protein_bind    complement(16703..16727)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggccctctag aggatccccg ggtaccctcg aattatcata catgagaatt aagggagtca
       61 cgttatgacc cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacagaacc
      121 gcaacgttga aggagccact cagccgcggg tttctggagt ttaatgagct aagcacatac
      181 gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc tagcaaatat
      241 ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc
      301 tcatattcac tctcaactcg atcgaggcat gattgaacaa gatggattgc acgcaggttc
      361 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
      421 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
      481 cgacctgtcc ggtgccctga atgaactcca agacgaggca gcgcggctat cgtggctggc
      541 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
      601 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
      661 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
      721 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
      781 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
      841 cgccaggctc aaggcgcgga tgcccgacgg cgaggatctc gtcgtgaccc acggcgatgc
      901 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
      961 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     1021 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     1081 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     1141 ggactctagc tagagtcaag cagatcgttc aaacatttgg caataaagtt tcttaagatt
     1201 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     1261 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     1321 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     1381 attatcgcgc gcggtgtcat ctatgttact agatcgaccg gcatgcaagc tgataattca
     1441 attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
     1501 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
     1561 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
     1621 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
     1681 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
     1741 acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc
     1801 tcgttcggtt cgtaagctgt aatgcaagta gcgtaactgc cgtcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttcttgttat
     1921 gacatgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     1981 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     2041 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     2101 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     2161 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     2221 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     2281 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     2341 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     2401 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     2461 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     2521 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     2581 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     2641 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     2701 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     2761 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     2821 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     2881 gccgcttcgc cggcgttaac tcaagcgatt agatgcacta agcacataat tgctcacagc
     2941 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca
     3001 aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3061 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3121 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3181 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3241 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3301 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3361 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3421 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3481 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3541 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3601 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3661 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3721 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3781 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3841 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     3901 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     3961 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     4021 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4081 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4141 cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc
     4201 cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga
     4261 taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt
     4321 gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag
     4381 ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag
     4441 tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc
     4501 ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt
     4561 ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac
     4621 cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct
     4681 gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca
     4741 cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga
     4801 acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc
     4861 cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc
     4921 cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc
     4981 cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca
     5041 ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc
     5101 gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact
     5161 tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc
     5221 ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc
     5281 acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt
     5341 agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca
     5401 tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt
     5461 gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg
     5521 attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct
     5581 cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca
     5641 gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gtcacgccga ttcctcgggc
     5701 ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa
     5761 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
     5821 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
     5881 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
     5941 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
     6001 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
     6061 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
     6121 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
     6181 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
     6241 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
     6301 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
     6361 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
     6421 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
     6481 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
     6541 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
     6601 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
     6661 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
     6721 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
     6781 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
     6841 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
     6901 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
     6961 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
     7021 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
     7081 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
     7141 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
     7201 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
     7261 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
     7321 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
     7381 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
     7441 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
     7501 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
     7561 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
     7621 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt
     7681 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct
     7741 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca
     7801 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc
     7861 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat
     7921 caaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac
     7981 ggataaacct tttcacgccc ttttaaatat ccgttattct aataaacgct cttttctctt
     8041 aggtttaccc gccaatatat cctgtcaaac actgatagtt taaactgaag gcgggaaacg
     8101 acaatctgat ccaagctcaa gctaagctcc tcagaagacc agagggctat tgagactttt
     8161 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     8221 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
     8281 aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc cccacccacg
     8341 aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
     8401 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     8461 tctatataag gaagttcatt tcatttggag aggacaggct tcttgagatc cttcaacaat
     8521 taccaacaac aacaaacaac aaacaacatt acaattacta tttacaatta cagtcgaccc
     8581 acgcgtccga taatttcccc aatttctcag agagactcaa aatcaccacc accaccaaat
     8641 cgccgccatg tttaaacaag cttctcgtct cctctcccga tctgtcgccg ccgcatcttc
     8701 caaatcggtg acgactcgtg ccttttcaac ggaacttcca tcgacgctcg attccgctag
     8761 tgatatcaca agtttgtaca aaaaagcagg cttttaccca tacgatgttc cagattacgc
     8821 tgatatcgcc gatctacgca cgctcggcta cagccagcag caacaggaga agatcaaacc
     8881 gaaggttcgt tcgacagtgg cgcagcacca cgaggcactg gtcggccacg ggtttacaca
     8941 cgcgcacatc gttgcgttaa gccaacaccc ggcagcgtta gggaccgtcg ctgtcaagta
     9001 tcaggacatg atcgcagcgt tgccagaggc gacacacgaa gcgatcgttg gcgtcggcaa
     9061 acagtggtcc ggcgcacgcg ctctggaggc cttgctcacg gtggcgggag agttgagagg
     9121 tccaccgtta cagttggaca caggccaact tctcaagatt gcaaaacgtg gcggcgtgac
     9181 cgcagtggag gcagtgcatg catggcgcaa tgcactgacg ggtgccccgc tcaacctgac
     9241 cccagaccag gttgtggcca tcgccagcaa cataggtggc aagcaggccc tcgaaaccgt
     9301 ccagagactg ttaccggttc tctgccagga ccacggcctg accccagaac aggttgtggc
     9361 catcgccagc aacataggtg gcaagcaggc cctcgaaacc gtccagagac tgttaccggt
     9421 tctctgccag gcccacggcc tgacccccga ccaggttgtc gctattgcta gtaacggcgg
     9481 aggcaaacag gcgctggaaa cagttcagcg cctcttgccg gtcttgtgtc aggcccacgg
     9541 cctgacccca gcccaagttg tcgcgattgc aagcaacaac ggaggcaaac aagccttaga
     9601 aacagtccag agattgttgc cggtgctgtg ccaagaccac ggcctgaccc cagaccaagt
     9661 tgtcgcgatt gcaagcaaca acggaggcaa acaagcctta gaaacagtcc agagattgtt
     9721 gccggtgctg tgccaagacc acggcctgac cccggaacag gtggttgcaa tcgcgtcaca
     9781 cgatggggga aagcaggccc tagaaaccgt tcagcgactc ctgcccgtcc tgtgccaggc
     9841 ccacggcctg accccggacc aggtggttgc aatcgcgtca cacgatgggg gaaagcaggc
     9901 cctagaaacc gttcagcgac tcctgcccgt cctgtgccag gcccacggcc tgaccccggc
     9961 ccaggtggtt gcaatcgcgt cacacgatgg gggaaagcag gccctagaaa ccgttcagcg
    10021 actcctgccc gtcctgtgcc aggaccacgg cctgacccca gaccaggttg tggccatcgc
    10081 cagcaacata ggtggcaagc aggccctcga aaccgtccag agactgttac cggttctctg
    10141 ccaggaccac ggcctgaccc ccgaacaggt tgtcgctatt gctagtaacg gcggaggcaa
    10201 acaggcgctg gaaacagttc agcgcctctt gccggtcttg tgtcaggccc acggcctgac
    10261 cccagaccag gttgtggcca tcgccagcaa cataggtggc aagcaggccc tcgaaaccgt
    10321 ccagagactg ttaccggttc tctgccaggc ccacggcctg acccccgccc aggttgtcgc
    10381 tattgctagt aacggcggag gcaaacaggc gctggaaaca gttcagcgcc tcttgccggt
    10441 cttgtgtcag gaccacggcc tgacccccga ccaggttgtc gctattgcta gtaacggcgg
    10501 aggcaaacag gcgctggaaa cagttcagcg cctcttgccg gtcttgtgtc aggaccacgg
    10561 cctgaccccc gaacaggttg tcgctattgc tagtaacggc ggaggcaaac aggcgctgga
    10621 aacagttcag cgcctcttgc cggtcttgtg tcaggcccac ggcctgaccc cagaccaagt
    10681 tgtcgcgatt gcaagcaaca acggaggcaa acaagcctta gaaacagtcc agagattgtt
    10741 gcctgtgctg tgccaagccc acggcctgac cccagcccaa gttgtcgcga ttgcaagcaa
    10801 caacggaggc aaacaagcct tagaaacagt ccagagattg ttgccggtgc tgtgccaaga
    10861 ccacggcctg accccggacc aggtggttgc aatcgcgtca cacgatgggg gaaagcaggc
    10921 cctagaaacc gttcagcgac tcctgcccgt cctgtgccag gaccacggcc tgacccccga
    10981 acaggttgtc gctattgcta gtaacggcgg aggcaaacag gcgctggaaa cagttcagcg
    11041 cctcttgccg gtcttgtgtc aggcccacgg cctgacccca gaccaggttg tggccatcgc
    11101 cagcaacata ggtggcaagc aggccctcga aaccgtccag agactgttac cggttctctg
    11161 ccaggcccac ggcacccctg agcaggtagt ggctattgca tccaacatag ggggcagacc
    11221 cgcactggag tcaattgttg cccagttatc tcgccctgat ccggcgttgg ccgcgttgac
    11281 caacgaccac ctcgtcgcct tggcctgcct cggcgggcgt cctgcgctgg atgcagtgaa
    11341 aaagggattg ggggatccta tcagccgttc ccagctggtg aagtccgagc tggaggagaa
    11401 gaaatccgag ttgaggcaca agctgaagta cgtgccccac gagtacatcg agctgatcga
    11461 gatcgcccgg aacagcaccc aggaccgtat cctggagatg aaggtgatgg agttcttcat
    11521 gaaggtgtac ggctacaggg gcaagcacct gggcggctcc aggaagcccg acggcgccat
    11581 ctacaccgtg ggctccccca tcgactacgg cgtgatcgtg gacaccaagg cctactccgg
    11641 cggctacaac ctgcccatcg gccaggccga cgaaatgcag aggtacgtgg aggagaacca
    11701 gaccaggaac aagcacatca accccaacga gtggtggaag gtgtacccct ccagcgtgac
    11761 cgagttcaag ttcctgttcg tgtccggcca cttcaagggc aactacaagg cccagctgac
    11821 caggctgaac cacatcacca actgcaacgg cgccgtgctg tccgtggagg agctcctgat
    11881 cggcggcgag atgatcaagg ccggcaccct gaccctggag gaggtgagga ggaagttcaa
    11941 caacggcgag atcaacttcg cggccgactg ataactcgag cgatcctcta gacacccaac
    12001 ttttctatac aaagttgtga atatgaagat gaagatgaaa tatttggtgt gtcaaataaa
    12061 aagcttgtgt gcttaagttt gtgttttttt cttggcttgt tgtgttatga atttgtggct
    12121 ttttctaata ttaaatgaat gtaagatctc attataatga ataaacaaat gtttctataa
    12181 tccattgtga atgttttgtt ggatctcttc tgcagcatat aactactgta tgtgctatgg
    12241 tatggactat ggaatatgat taaagataag atgggctcat agagtaaaac gaggcgaggg
    12301 acctataaac ctcccttcat catgctattt catgatctat tttataaaat aaagatgtag
    12361 aaaaaagtaa gcgtaataac cgcaaaacaa atgatttaaa acatggcaca taatgaggag
    12421 attaagttcg gtttacgttt attttagtac taattgtaac gtgagactac gtatcgggaa
    12481 tcgcctaatt aaagcattaa tgcgaacctg attagattca ccgaccctcc tatcgtgtcg
    12541 acctttctgt ttcttagaat tttttggtag tctatgtact aataatgtca gcttcgtatt
    12601 tatttcataa gcaatttgca tttgcaattt gttttttact tttattttta ttgtattgtg
    12661 gaatgtggac tcgtaccaac atgaagttat ataccaccaa aaaaattaca gttagtcaaa
    12721 agattcacga gtgagagcta cttatgattg tcttttacgt atatgtctaa ttgtctattt
    12781 gctcaataat ctttgtactt tcttttgtcg ttgataaatc tagaggatcc cccctcagaa
    12841 gaccagaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
    12901 cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg tggctcctac
    12961 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctac cgacagtggt
    13021 cccaaagatg gacccccacc cacgaggaac atcgtggaaa aagaagacgt tccaaccacg
    13081 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
    13141 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca
    13201 ggcttcttga gatccttcaa caattaccaa caacaacaaa caacaaacaa cattacaatt
    13261 actatttaca attacagtcg acccacgcgt aagataattt ccccaatttc tcagagagac
    13321 tcaaaatcac caccaccacc aaatcgccgc catgtttaaa caagcttctc gtctcctctc
    13381 ccgatctgtc gccgccgcat cttccaaatc ggtgacgact cgtgcctttt caacggaact
    13441 tccatcgacg ctcgattcca caactttgta taataaagtt gataaggaga ccgccgctgc
    13501 caagttcgag agacagcaca tggacagcat cgatatcgcc gatctacgca cgctcggcta
    13561 cagccagcag caacaggaga agatcaaacc gaaggttcgt tcgacagtgg cgcagcacca
    13621 cgaggcactg gtcggccacg ggtttacaca cgcgcacatc gttgcgttaa gccaacaccc
    13681 ggcagcgtta gggaccgtcg ctgtcaagta tcaggacatg atcgcagcgt tgccagaggc
    13741 gacacacgaa gcgatcgttg gcgtcggcaa acagtggtcc ggcgcacgcg ctctggaggc
    13801 cttgctcacg gtggcgggag agttgagagg tccaccgtta cagttggaca caggccaact
    13861 tctcaagatt gcaaaacgtg gcggcgtgac cgcagtggag gcagtgcatg catggcgcaa
    13921 tgcactgacg ggtgccccgc tcaacctgac cccagaccaa gttgtcgcga ttgcaagcaa
    13981 caacggaggc aaacaagcct tagaaacagt ccagagattg ttgccggtgc tgtgccaaga
    14041 ccacggcctg accccagaac aagttgtcgc gattgcaagc aacaacggag gcaaacaagc
    14101 cttagaaaca gtccagagat tgttgccggt gctgtgccaa gcccacggcc tgacccccga
    14161 ccaggttgtc gctattgcta gtaacggcgg aggcaaacag gcgctggaaa cagttcagcg
    14221 cctcttgccg gtcttgtgtc aggcccacgg cctgaccccc gcccaggttg tcgctattgc
    14281 tagtaacggc ggaggcaaac aggcgctgga aacagttcag cgcctcttgc cggtcttgtg
    14341 tcaggaccac ggcctgaccc cggaccaggt ggttgcaatc gcgtcacacg atgggggaaa
    14401 gcaggcccta gaaaccgttc agcgactcct gcccgtcctg tgccaggacc acggcctgac
    14461 cccagaacaa gttgtcgcga ttgcaagcaa caacggaggc aaacaagcct tagaaacagt
    14521 ccagagattg ttgccggtgc tgtgccaagc ccacggcctg acccccgacc aggttgtcgc
    14581 tattgctagt aacggcggag gcaaacaggc gctggaaaca gttcagcgcc tcttgccggt
    14641 cttgtgtcag gcccacggcc tgaccccagc ccaggttgtg gccatcgcca gcaacatagg
    14701 tggcaagcag gccctcgaaa ccgtccagag actgttaccg gttctctgcc aggaccacgg
    14761 cctgacccca gaccaggttg tggccatcgc cagcaacata ggtggcaagc aggccctcga
    14821 aaccgtccag agactgttac cggttctctg ccaggaccac ggcctgaccc cagaacaggt
    14881 tgtggccatc gccagcaaca taggtggcaa gcaggccctc gaaaccgtcc agagactgtt
    14941 accggttctc tgccaggccc acggcctgac cccggaccag gtggttgcaa tcgcgtcaca
    15001 cgatggggga aagcaggccc tagaaaccgt tcagcgactc ctgcccgtcc tgtgccaggc
    15061 ccacggcctg accccagccc aggttgtggc catcgccagc aacataggtg gcaagcaggc
    15121 cctcgaaacc gtccagagac tgttaccggt tctctgccag gaccacggcc tgaccccaga
    15181 ccaggttgtg gccatcgcca gcaacatagg tggcaagcag gccctcgaaa ccgtccagag
    15241 actgttaccg gttctctgcc aggaccacgg cctgaccccc gaacaggttg tcgctattgc
    15301 tagtaacggc ggaggcaaac aggcgctgga aacagttcag cgcctcttgc cggtcttgtg
    15361 tcaggcccac ggcctgaccc cagaccaagt tgtcgcgatt gcaagcaaca acggaggcaa
    15421 acaagcctta gaaacagtcc agagattgtt gcctgtgctg tgccaagccc acggcctgac
    15481 ccccgcccag gttgtcgcta ttgctagtaa cggcggaggc aaacaggcgc tggaaacagt
    15541 tcagcgcctc ttgccggtct tgtgtcagga ccacggcctg acccccgacc aggttgtcgc
    15601 tattgctagt aacggcggag gcaaacaggc gctggaaaca gttcagcgcc tcttgccggt
    15661 cttgtgtcag gaccacggcc tgaccccaga acaagttgtc gcgattgcaa gcaacaacgg
    15721 aggcaaacaa gccttagaaa cagtccagag attgttgccg gtgctgtgcc aagcccacgg
    15781 cctgacccca gaccaagttg tcgcgattgc aagcaacaac ggaggcaaac aagccttaga
    15841 aacagtccag agattgttgc ctgtgctgtg ccaagcccac ggcacccctg agcaggtagt
    15901 ggctattgca tccaacggag ggggcagacc cgcactggag tcaattgttg cccagttatc
    15961 tcgccctgat ccggcgttgg ccgcgttgac caacgaccac ctcgtcgcct tggcctgcct
    16021 cggcgggcgt cctgcgctgg atgcagtgaa aaagggattg ggggatccta tcagccgttc
    16081 ccagctggtg aagtccgagc tggaggagaa gaaatccgag ttgaggcaca agctgaagta
    16141 cgtgccccac gagtacatcg agctgatcga gatcgcccgg aacagcaccc aggaccgtat
    16201 cctggagatg aaggtgatgg agttcttcat gaaggtgtac ggctacaggg gcaagcacct
    16261 gggcggctcc aggaagcccg acggcgccat ctacaccgtg ggctccccca tcgactacgg
    16321 cgtgatcgtg gacaccaagg cctactccgg cggctacaac ctgcccatcg gccaggccga
    16381 cgaaatgcag aggtacgtgg aggagaacca gaccaggaac aagcacatca accccaacga
    16441 gtggtggaag gtgtacccct ccagcgtgac cgagttcaag ttcctgttcg tgtccggcca
    16501 cttcaagggc aactacaagg cccagctgac caggctgaac cacatcacca actgcaacgg
    16561 cgccgtgctg tccgtggagg agctcctgat cggcggcgag atgatcaagg ccggcaccct
    16621 gaccctggag gaggtgagga ggaagttcaa caacggcgag atcaacttcg cggccgactg
    16681 ataactcgag cgatcctcta gaacccagct ttcttgtaca aagtggtgat atcccgcggc
    16741 catgctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt
    16801 ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg
    16861 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
    16921 tcaataaaat ttctaattcc taaaaccaaa atccagtgac ctgcaggcat gcgacgtcg
//
