LOCUS       sequence-306378-        9497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle vector for expression dCas9, mobilization cluster, expresses
            the tracrRNA and a CRISPR array designed for the easy cloning of new
            spacers..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Tan SI, Yu PJ, Ng IS
  TITLE     CRISPRi-mediated Programming Essential Gene can as a Direct 
  JOURNAL   Biotechnol Bioeng. 2020 May 27. doi: 10.1002/bit.27443.
  PUBMED    32458463
REFERENCE   2  (bases 1 to 9497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2020 May 27. doi: 10.1002/bit.27443."
COMMENT     Sequence Label: pSM-dCas9::Ap
FEATURES             Location/Qualifiers
     source          1..9497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(149..175,176..178,179..2665,2666..2668,2669..4255)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4276..4407
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   4408..4443
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   4474..4509
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     primer_bind     complement(4938..4957)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        5163..5226
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5227..5295,5296..6087)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5445..5464)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            6307..6416
                     /note="incP origin of transfer"
     CDS             6449..6820
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKAMMGVVRPRAEP"
     terminator      7759..7816
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(8135..8152)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8269..8814)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8349..8368)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(8926..8931,8932..8948,8949..8954)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_RNA        complement(9272..9350)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
ORIGIN
        1 taagtattgg gtaatatttt ttgaagagat attttgaaaa agaaaaatta aagcatatta
       61 aactaatttc ggaggtcatt aaaactatta ttgaaatcat caaactcatt atggatttaa
      121 tttaaacttt ttattttagg aggcaaaaat ggataagaaa tactcaatag gcttagctat
      181 cggcacaaat agcgtcggat gggcggtgat cactgatgaa tataaggttc cgtctaaaaa
      241 gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa aaaaatctta taggggctct
      301 tttatttgac agtggagaga cagcggaagc gactcgtctc aaacggacag ctcgtagaag
      361 gtatacacgt cggaagaatc gtatttgtta tctacaggag attttttcaa atgagatggc
      421 gaaagtagat gatagtttct ttcatcgact tgaagagtct tttttggtgg aagaagacaa
      481 gaagcatgaa cgtcatccta tttttggaaa tatagtagat gaagttgctt atcatgagaa
      541 atatccaact atctatcatc tgcgaaaaaa attggtagat tctactgata aagcggattt
      601 gcgcttaatc tatttggcct tagcgcatat gattaagttt cgtggtcatt ttttgattga
      661 gggagattta aatcctgata atagtgatgt ggacaaacta tttatccagt tggtacaaac
      721 ctacaatcaa ttatttgaag aaaaccctat taacgcaagt ggagtagatg ctaaagcgat
      781 tctttctgca cgattgagta aatcaagacg attagaaaat ctcattgctc agctccccgg
      841 tgagaagaaa aatggcttat ttgggaatct cattgctttg tcattgggtt tgacccctaa
      901 ttttaaatca aattttgatt tggcagaaga tgctaaatta cagctttcaa aagatactta
      961 cgatgatgat ttagataatt tattggcgca aattggagat caatatgctg atttgttttt
     1021 ggcagctaag aatttatcag atgctatttt actttcagat atcctaagag taaatactga
     1081 aataactaag gctcccctat cagcttcaat gattaaacgc tacgatgaac atcatcaaga
     1141 cttgactctt ttaaaagctt tagttcgaca acaacttcca gaaaagtata aagaaatctt
     1201 ttttgatcaa tcaaaaaacg gatatgcagg ttatattgat gggggagcta gccaagaaga
     1261 attttataaa tttatcaaac caattttaga aaaaatggat ggtactgagg aattattggt
     1321 gaaactaaat cgtgaagatt tgctgcgcaa gcaacggacc tttgacaacg gctctattcc
     1381 ccatcaaatt cacttgggtg agctgcatgc tattttgaga agacaagaag acttttatcc
     1441 atttttaaaa gacaatcgtg agaagattga aaaaatcttg acttttcgaa ttccttatta
     1501 tgttggtcca ttggcgcgtg gcaatagtcg ttttgcatgg atgactcgga agtctgaaga
     1561 aacaattacc ccatggaatt ttgaagaagt tgtcgataaa ggtgcttcag ctcaatcatt
     1621 tattgaacgc atgacaaact ttgataaaaa tcttccaaat gaaaaagtac taccaaaaca
     1681 tagtttgctt tatgagtatt ttacggttta taacgaattg acaaaggtca aatatgttac
     1741 tgaaggaatg cgaaaaccag catttctttc aggtgaacag aagaaagcca ttgttgattt
     1801 actcttcaaa acaaatcgaa aagtaaccgt taagcaatta aaagaagatt atttcaaaaa
     1861 aatagaatgt tttgatagtg ttgaaatttc aggagttgaa gatagattta atgcttcatt
     1921 aggtacctac catgatttgc taaaaattat taaagataaa gattttttgg ataatgaaga
     1981 aaatgaagat atcttagagg atattgtttt aacattgacc ttatttgaag atagggagat
     2041 gattgaggaa agacttaaaa catatgctca cctctttgat gataaggtga tgaaacagct
     2101 taaacgtcgc cgttatactg gttggggacg tttgtctcga aaattgatta atggtattag
     2161 ggataagcaa tctggcaaaa caatattaga ttttttgaaa tcagatggtt ttgccaatcg
     2221 caattttatg cagctgatcc atgatgatag tttgacattt aaagaagaca ttcaaaaagc
     2281 acaagtgtct ggacaaggcg atagtttaca tgaacatatt gcaaatttag ctggtagccc
     2341 tgctattaaa aaaggtattt tacagactgt aaaagttgtt gatgaattgg tcaaagtaat
     2401 ggggcggcat aagccagaaa atatcgttat tgaaatggca cgtgaaaatc agacaactca
     2461 aaagggccag aaaaattcgc gagagcgtat gaaacgaatc gaagaaggta tcaaagaatt
     2521 aggaagtcag attcttaaag agcatcctgt tgaaaatact caattgcaaa atgaaaagct
     2581 ctatctctat tatctccaaa atggaagaga catgtatgtg gaccaagaat tagatattaa
     2641 tcgtttaagt gattatgatg tcgatgccat tgttccacaa agtttcctta aagacgattc
     2701 aatagacaat aaggtcttaa cgcgttctga taaaaatcgt ggtaaatcgg ataacgttcc
     2761 aagtgaagaa gtagtcaaaa agatgaaaaa ctattggaga caacttctaa acgccaagtt
     2821 aatcactcaa cgtaagtttg ataatttaac gaaagctgaa cgtggaggtt tgagtgaact
     2881 tgataaagct ggttttatca aacgccaatt ggttgaaact cgccaaatca ctaagcatgt
     2941 ggcacaaatt ttggatagtc gcatgaatac taaatacgat gaaaatgata aacttattcg
     3001 agaggttaaa gtgattacct taaaatctaa attagtttct gacttccgaa aagatttcca
     3061 attctataaa gtacgtgaga ttaacaatta ccatcatgcc catgatgcgt atctaaatgc
     3121 cgtcgttgga actgctttga ttaagaaata tccaaaactt gaatcggagt ttgtctatgg
     3181 tgattataaa gtttatgatg ttcgtaaaat gattgctaag tctgagcaag aaataggcaa
     3241 agcaaccgca aaatatttct tttactctaa tatcatgaac ttcttcaaaa cagaaattac
     3301 acttgcaaat ggagagattc gcaaacgccc tctaatcgaa actaatgggg aaactggaga
     3361 aattgtctgg gataaagggc gagattttgc cacagtgcgc aaagtattgt ccatgcccca
     3421 agtcaatatt gtcaagaaaa cagaagtaca gacaggcgga ttctccaagg agtcaatttt
     3481 accaaaaaga aattcggaca agcttattgc tcgtaaaaaa gactgggatc caaaaaaata
     3541 tggtggtttt gatagtccaa cggtagctta ttcagtccta gtggttgcta aggtggaaaa
     3601 agggaaatcg aagaagttaa aatccgttaa agagttacta gggatcacaa ttatggaaag
     3661 aagttccttt gaaaaaaatc cgattgactt tttagaagct aaaggatata aggaagttaa
     3721 aaaagactta atcattaaac tacctaaata tagtcttttt gagttagaaa acggtcgtaa
     3781 acggatgctg gctagtgccg gagaattaca aaaaggaaat gagctggctc tgccaagcaa
     3841 atatgtgaat tttttatatt tagctagtca ttatgaaaag ttgaagggta gtccagaaga
     3901 taacgaacaa aaacaattgt ttgtggagca gcataagcat tatttagatg agattattga
     3961 gcaaatcagt gaattttcta agcgtgttat tttagcagat gccaatttag ataaagttct
     4021 tagtgcatat aacaaacata gagacaaacc aatacgtgaa caagcagaaa atattattca
     4081 tttatttacg ttgacgaatc ttggagctcc cgctgctttt aaatattttg atacaacaat
     4141 tgatcgtaaa cgatatacgt ctacaaaaga agttttagat gccactctta tccatcaatc
     4201 catcactggt ctttatgaaa cacgcattga tttgagtcag ctaggaggtg actgaagtat
     4261 attttagatg aagattattt cttaataact aaaaatatgg tataatactc ttaataaatg
     4321 cagtaataca ggggcttttc aagactgaag tctagctgag acaaatagtg cgattacgaa
     4381 attttttaga caaaaatagt ctacgaggtt ttagagctat gctgttttga atggtcccaa
     4441 aactgagacc agtctcggaa gctcaaaggt ctcgttttag agctatgctg ttttgaatgg
     4501 tcccaaaact tcagcacact gagacttgtt gagttccatg ttttagagct atgctgtttt
     4561 gaatggactc cattcaacat tgccgatgat aacttgagaa agagggttaa taccagcagt
     4621 cggatacctt cctattcttt ctgttaaagc gttttcatgt tataataggc aaaagaagag
     4681 tagtgtgatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     4741 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     4801 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     4861 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     4921 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     4981 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     5041 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     5101 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgacttca
     5161 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5221 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5281 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5341 ggtgcgcgag tgggttacat cgaactggat ctcaayagcg gtaagatcct tgagagtttt
     5401 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5461 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5521 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5581 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5641 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5701 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5761 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5821 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5881 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5941 gggtcacgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6001 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6061 ggtgcctcac tgattaagca ttggtaactc gaggtcccct cagttcagta atttcctgca
     6121 tttgcctgtt tccagtcggt agatattcca caaaacagca gggaagcagc gcttttccgc
     6181 tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc
     6241 acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg
     6301 tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt
     6361 cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccggctac
     6421 cgccggcgta acagatgagg gcaagcggat ggctgatgaa accaagccaa ccaggaaggg
     6481 cagcccacct atcaaggtgt actgccttcc agacgaacga agagcgattg aggaaaaggc
     6541 ggcggcggcc ggcatgagcc tgtcggccta cctgctggcc gtcggccagg gctacaaaat
     6601 cacgggcgtc gtggactatg agcacgtccg cgagctggcc cgcatcaatg gcgacctggg
     6661 ccgcctgggc ggcctgctga aactctggct caccgacgac ccgcgcacgg cgcggttcgg
     6721 tgatgccacg atcctcgccc tgctggcgaa gatcgaagag aagcaggacg agcttggcaa
     6781 ggccatgatg ggcgtggtcc gcccgagggc agagccatga cttttttagc cgctaaaacg
     6841 gccggggggt gcgcgtgatt gccaagcacg tccccatgcg ctccatcaag aagagcgact
     6901 tcgcggagct ggtgaagtac atcaccgacg agcaaggcaa gaccgagcgc ctgggtcacg
     6961 tgcgcgtcac gaactgcgag gcaaacaccc tgcccgctgt catggccgag gtgatggcga
     7021 cccagtacgg caacacccgt tccgaggccg acaagaccta tcacctgctg gttagcttcc
     7081 gcgcgggaga gaagcccgac gcggagacgt tgcgcgcgat tgaggaccgc atctgcgctg
     7141 ggcttggctt cgccgagcat cagcgcgtca gtgccgtgca tcacgacacc gacaacctgc
     7201 acatccatat cgccatcaac aagattcacc cgacccgaaa caccatccat gagccgtatc
     7261 gggcctaccg cgccctcgct gacctctgcg cgacgctcga acgggactac gggcttgagc
     7321 gtgacaatca cgaaacgcgg cagcgcgttt ccgagaaccg cgcgaacgac atggagcggc
     7381 acgcgggcgt ggaaagcctg gtcggctgga tccggccacg atgcgtccgg cgtagaggat
     7441 ctgaagatca gcagttcaac ctgttgatag tacgtactaa gctctcatgt ttcacgtact
     7501 aagctctcat gtttaacgta ctaagctctc atgtttaacg aactaaaccc tcatggctaa
     7561 cgtactaagc tctcatggct aacgtactaa gctctcatgt ttcacgtact aagctctcat
     7621 gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg ttttaagttt
     7681 tataagaaaa aaaagaatat ataaggcttt taaagctttt aaggtttaac ggttgtggac
     7741 tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     7801 tttaaaaccg aaaagattac ttcgcgttat gcagatctta tttattcggc gcaaagtgcg
     7861 tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt
     7921 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg
     7981 caaaagcacc gccggacatc agcgctagcg gagtgtatac tggcttacta tgttggcact
     8041 gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc
     8101 agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg
     8161 tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg
     8221 ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct
     8281 ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac
     8341 aggactataa agataccagg cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc
     8401 tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc
     8461 tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg
     8521 ttcagtccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac
     8581 atgcaaaggc accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag
     8641 tcatgcgccg gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc
     8701 cagttacctc ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag
     8761 gcggtttttt cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat
     8821 catcttatca atcagataaa atatttctag atttcagtgc aatttatctc ttcaaatgta
     8881 gcacctgaag tcagccccat acgatataag ttgtaattct aatgtttgac agcttatcat
     8941 cgataagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg
     9001 tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg
     9061 cataggcttg gttatgccgg tactgccggg cctcttgcgg gattacgaaa tcatcctgtg
     9121 gagcttagta ggtttagcaa gatggcagcg cctaaatgta gaatgataaa aggattaaga
     9181 gattaatttc cctaaaaatg ataaaacaag cgttttgaaa gcgcttgttt ttttggtttg
     9241 cagtcagagt agaatagaag tatcaaaaaa agcaccgact cggtgccact ttttcaagtt
     9301 gataacggac tagccttatt ttaacttgct atgctgtttt gaatggttcc aacaagatta
     9361 ttttataact tttataacaa ataatcaagg agaaattcaa agaaatttat cagccataaa
     9421 acaatactta atactataga atgataacaa aataaactac tttttaaaag aattttgtgt
     9481 tataatctat ttattat
//
