LOCUS       sequence-306404-        2637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed
            by a linker, the tail region of AnkyrinG, and an ER export motif 
            (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Shemesh OA, Linghu C, Piatkevich KD, Goodwin D, Celiker OT, Gritton 
            HJ, Romano MF, Gao R, Yu CJ, Tseng HA, Bensussen S, Narayan S, Yang 
            CT, Freifeld L, Siciliano CA, Gupta I, Wang J, Pak N, Yoon YG, 
            Ullmann JFP, Guner-Ataman B, Noamany H, Sheinkopf ZR, Park WM, Asano
            S, Keating AE, Trimmer JS, Reimer J, Tolias AS, Bear MF, Tye KM, Han
            X, Ahrens MB, Boyden ES
  TITLE     Precision Calcium Imaging of Dense Neural Populations via a 
            Cell-Body-Targeted Calcium Indicator.
  JOURNAL   Neuron. 2020 Jun 26. pii: S0896-6273(20)30398-6. doi: 
            10.1016/j.neuron.2020.05.029.
  PUBMED    32592656
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2020 Jun 26. pii: S0896-6273(20)30398-6. doi: 
            10.1016/j.neuron.2020.05.029."
COMMENT     Sequence Label: pAAV-Syn-SomaGCaMP6f1
FEATURES             Location/Qualifiers
     source          1..2637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..9,10..27,28..30,31..63,64..66,67..90,91..117,
                     118..174,175..180,181..450,451..468,469..903,904..909,
                     910..1350)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      97..106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     387..408
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(514..535)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(775..794)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgggttctc atcatcatca tcatcatggt atggcttcca tgactggtgg acagcaaatg
       61 ggtcgggatc tgtacgacga tgacgataag gatctcgcca ccatggtcga ctcatcacgt
      121 cgtaagtgga ataagacagg tcacgcagtc agagctatag gtcggctgag ttcactcgag
      181 aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt caagatccgc
      241 cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc
      301 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc caaactttcg
      361 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      421 atcactctcg gcatggacga gctgtacaag ggcggaaccg gagggagcat ggtgagcaag
      481 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      541 ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc
      601 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      661 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      721 ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt caaggacgac
      781 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      841 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      901 aacctgccgg accaactgac tgaagagcag atcgcagaat ttaaagagga gttctcccta
      961 tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt gatgcggtct
     1021 ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac
     1081 ggtgacggca caatcgactt ccctgagttc ctgacaatga tggcaagaaa aatgaaatac
     1141 agggacacgg aagaagaaat tagagaagcg ttcggtgtgt ttgataagga tggcaatggc
     1201 tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa gttaacagat
     1261 gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca ggtaaactac
     1321 gaagagtttg tacaaatgat gacagcgaag gagctcggcg gatctggcgg tagcggaggg
     1381 acaggtggct ctggagggag cggaggcact ggcggatctg gtgggagcgg aggtaccaga
     1441 gaagggagaa tagacgatga agaacctttc aaaatcgttg agaaagtgaa ggaagactta
     1501 gtcaaagtca gtgagatact caaaaaggac gtgtgtgtcg agagcaaagg gccacccaag
     1561 tccccgaaga gtgacaaagg acactctcca gaggatgact ggacagaatt tagttctgag
     1621 gaaatacgag aagccaggca ggctgcggcc agtcacgccc cgtccctgcc tgagagagta
     1681 cacgggaagg ccaacctcac tagagtcata gactacctga ccaatgacat cgggagcagc
     1741 tcactgacca acttaaagta caagtttgaa gaggctaaga aggatggaga ggagagacag
     1801 aagaggattt taaagccagc catggccttg caggagcata aactcaaaat gccgccggcc
     1861 tccatgaggc cttccacctc tgagaaggaa ctgtgcaaga tggctgactc tttttttgga
     1921 gcagatgcca tcctagagtc tcccgatgac ttttcccagc atgaccaaga taaaagtccc
     1981 ttgtctgaca gtggctttga aacccgcagt gagaaaacac cttcggcccc gcaaagtgcc
     2041 gagagcacag gccctaagcc gttatttcac gaagtcccca tcccccctgt catcacagag
     2101 acaagaactg aagtggtcca tgtgatcagg agctatgagc cctcttctgg agagatcccc
     2161 cagtcccagc cagaggaccc cgtgtctccc aagccttccc ctacttttat ggagttggaa
     2221 ccaaaaccca ccacctctag catcaaggaa aaagtaaaag catttcagat gaaagccagt
     2281 agtgaggaag aggaccacag tcgggttcta agcaaaggca tgcgtgtcaa agaagagact
     2341 cacatcacca cgaccaccag gatggtgtat cactcccctc ctggtggcga gtgcgcctcc
     2401 gaaaggattg aggagaccat gtcagtccat gacatcatga aggcctttca gtccgggcga
     2461 gacccttcca aagagctggc agggctgttt gaacacaagt cggccatgtc tccagatgtt
     2521 gccaagtctg ctgctgagac ctcagcccag cacgcagaga aggacagcca aatgaaaccc
     2581 aaactggagc gcattataga agtgcacatc gaaaaaggtc cgcagagtcc ttgtgag
//
