LOCUS       sequence-306407-        2457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cell body-targeted GCaMP6f, under synapsin promoter: nullCoChR (the 
            optogenetic protein CoChR mutated to have 0 current), followed by 
            GCaMP6f and the KV2.1 motif (nullCoChR-GCaMP6f-Kv2.1)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Shemesh OA, Linghu C, Piatkevich KD, Goodwin D, Celiker OT, Gritton 
            HJ, Romano MF, Gao R, Yu CJ, Tseng HA, Bensussen S, Narayan S, Yang 
            CT, Freifeld L, Siciliano CA, Gupta I, Wang J, Pak N, Yoon YG, 
            Ullmann JFP, Guner-Ataman B, Noamany H, Sheinkopf ZR, Park WM, Asano
            S, Keating AE, Trimmer JS, Reimer J, Tolias AS, Bear MF, Tye KM, Han
            X, Ahrens MB, Boyden ES
  TITLE     Precision Calcium Imaging of Dense Neural Populations via a 
            Cell-Body-Targeted Calcium Indicator.
  JOURNAL   Neuron. 2020 Jun 26. pii: S0896-6273(20)30398-6. doi: 
            10.1016/j.neuron.2020.05.029.
  PUBMED    32592656
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2020 Jun 26. pii: S0896-6273(20)30398-6. doi: 
            10.1016/j.neuron.2020.05.029."
COMMENT     Sequence Label: pAAV-Syn-nullCoChR-GCaMP6f-Kv2.1
FEATURES             Location/Qualifiers
     source          1..2457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..864
                     /codon_start=1
                     /product="high-photocurrent channelrhodopsin from 
                     Chloromonas oogama (Shemesh et al., 2017)"
                     /label=CoChR
                     /translation="MLGNGSAIVPIDQCFCLAWTDSLGSDTEQLVANILQWFAFGFSIL
                     ILMFYAYQTWRATCGWEEVYVCCVELTKVIIEFFHEFDDPSMLYLANGHRVQWLRYAEW
                     LLTCPVILIHLSNLTGLKDDYSKRTMRLLVSDVGTIVWGATSAMSTGYVKVIFFVLGCI
                     YGANTFFHAAKVYIESYHVVPKGRPRTVVRIMAWLFFLSWGMFPVLFVVGPEGFDAISV
                     YGSTIGHTIIDLMSANCWGLLGHYLRVLIHQHIIIYGDIRKKTKINVAGEEMEVETMVD
                     QEDEETV"
     CDS             join(901..909,910..927,928..930,931..963,964..966,967..990,
                     991..1017,1018..1074,1075..1080,1081..1350,1351..1368,
                     1369..1803,1804..1809,1810..2250)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      997..1006
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1287..1308
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1414..1435)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1675..1694)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgctgggga acggcagcgc cattgtgcct atcgaccagt gcttttgcct ggcttggacc
       61 gacagcctgg gaagcgatac agagcagctg gtggccaaca tcctccagtg gttcgccttc
      121 ggcttcagca tcctgatcct gatgttctac gcctaccaga cttggagagc cacttgcggt
      181 tgggaggagg tctacgtctg ttgcgtcgag ctgaccaagg tcatcatcga gttcttccac
      241 gagttcgacg accccagcat gctgtacctg gctaacggac accgagtcca gtggctgaga
      301 tacgcagagt ggctgctgac ttgtcccgtc atcctgatcc acctgagcaa cctgaccggc
      361 ctgaaggacg actacagcaa gcggaccatg aggctgctgg tgtcagacgt gggaaccatc
      421 gtgtggggag ctacaagcgc catgagcaca ggctacgtca aggtcatctt cttcgtgctg
      481 ggttgcatct acggcgccaa caccttcttc cacgccgcca aggtgtatat cgagagctac
      541 cacgtggtgc caaagggcag acctagaacc gtcgtgcgga tcatggcttg gctgttcttc
      601 ctgtcttggg gcatgttccc cgtgctgttc gtcgtgggac cagaaggatt cgacgccatc
      661 agcgtgtacg gctctaccat tggccacacc atcatcgacc tcatgagcgc taattgttgg
      721 ggcctgctgg gacactatct gagagtgctg atccaccagc acatcatcat ctacggcgac
      781 atccggaaga agaccaagat caacgtggcc ggcgaggaga tggaagtgga gaccatggtg
      841 gaccaggagg acgaggagac agtgggaggt tcaggtggaa ccggtggaag tggaggtacc
      901 atgggttctc atcatcatca tcatcatggt atggcttcca tgactggtgg acagcaaatg
      961 ggtcgggatc tgtacgacga tgacgataag gatctcgcca ccatggtcga ctcatcacgt
     1021 cgtaagtgga ataagacagg tcacgcagtc agagctatag gtcggctgag ttcactcgag
     1081 aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt caagatccgc
     1141 cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc
     1201 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc caaactttcg
     1261 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1321 atcactctcg gcatggacga gctgtacaag ggcggaaccg gagggagcat ggtgagcaag
     1381 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1441 ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc
     1501 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1561 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1621 ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt caaggacgac
     1681 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1741 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1801 aacctgccgg accaactgac tgaagagcag atcgcagaat ttaaagagga gttctcccta
     1861 tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt gatgcggtct
     1921 ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac
     1981 ggtgacggca caatcgactt ccctgagttc ctgacaatga tggcaagaaa aatgaaatac
     2041 agggacacgg aagaagaaat tagagaagcg ttcggtgtgt ttgataagga tggcaatggc
     2101 tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa gttaacagat
     2161 gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca ggtaaactac
     2221 gaagagtttg tacaaatgat gacagcgaag ggtgctagcc agagccagcc tatcctgaac
     2281 accaaggaga tggcccctca gagcaagcct cctgaggagc tggagatgag cagcatgcct
     2341 agccctgtgg cccctctgcc tgccaggacc gagggcgtga tcgacatgag gagcatgagc
     2401 agcatcgaca gcttcatcag ctgcgccacc gacttccctg aggccaccag gttctaa
//
