LOCUS       sequence-306411-        8040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing firefly luciferase (Luc2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   Ellinger B, Pohlmann D, Woens J, Jakel FM, Reinshagen J, Stocking C,
            Prassolov VS, Fehse B, Riecken K
  TITLE     A High-Throughput HIV-1 Drug Screening Platform, Based on Lentiviral
            Vectors and Compatible with Biosafety Level-1.
  JOURNAL   Viruses. 2020 May 25;12(5). pii: v12050580. doi: 10.3390/v12050580.
  PUBMED    32466195
REFERENCE   2  (bases 1 to 8040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Viruses."; 
            date: "2020-05-25"; volume: "12(5). pii: v12050580. doi"; pages: " 
            10.3390/v12050580"
COMMENT     Sequence Label: LeGO-Luc2
FEATURES             Location/Qualifiers
     source          1..8040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    106..339
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             524..568
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    866..983
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1036..1052
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1094..1127)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1198..1604
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      1616..1625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1622..3274
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    3351..3939
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3404..3424)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3822..3833)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3942..3958)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             4468..4648
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4710..5298)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4790..4809)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5469..6260,6261..6329))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6092..6111
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6330..6434)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6509..6528)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6700..7079
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7081..7279
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7234..7254
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7297..7477
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7524..7649
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
       61 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
      121 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
      181 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
      241 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
      301 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
      361 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
      421 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
      481 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
      541 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
      601 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
      661 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
      721 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
      781 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
      841 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
      901 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
      961 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     1021 tagtaccggg cccgccgagg tcgacggtat cgataagctc gcttcacgag attccagcag
     1081 gtcgagggac ctaataactt cgtatagcat acattatacg aagttatatt aagggttcca
     1141 agcttaagcg gccggccgct gaaagacccc acctgtaggt ttggcaagct agctgcagta
     1201 acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg
     1261 gcgggtacat gaaaatagct aacgttgggc caaacaggat atctgcggtg agcagtttcg
     1321 gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa
     1381 gaacagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt
     1441 ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc
     1501 ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac
     1561 ccctcactcg gcgcgccagt cctccgattg actgagtcgc ccggatccga tatccgccac
     1621 catggaagat gccaaaaaca ttaagaaggg cccagcgcca ttctacccac tcgaagacgg
     1681 gaccgccggc gagcagctgc acaaagccat gaagcgctac gccctggtgc ccggcaccat
     1741 cgcctttacc gacgcacata tcgaggtgga cattacctac gccgagtact tcgagatgag
     1801 cgttcggctg gcagaagcta tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt
     1861 gtgcagcgag aatagcttgc agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt
     1921 ggctgtggcc ccagctaacg acatctacaa cgagcgcgag ctgctgaaca gcatgggcat
     1981 cagccagccc accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca
     2041 aaagaagcta ccgatcatac aaaagatcat catcatggat agcaagaccg actaccaggg
     2101 cttccaaagc atgtacacct tcgtgacttc ccatttgcca cccggcttca acgagtacga
     2161 cttcgtgccc gagagcttcg accgggacaa aaccatcgcc ctgatcatga acagtagtgg
     2221 cagtaccgga ttgcccaagg gcgtagccct accgcaccgc accgcttgtg tccgattcag
     2281 tcatgcccgc gaccccatct tcggcaacca gatcatcccc gacaccgcta tcctcagcgt
     2341 ggtgccattt caccacggct tcggcatgtt caccacgctg ggctacttga tctgcggctt
     2401 tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc ttgcgcagct tgcaagacta
     2461 taagattcaa tctgccctgc tggtgcccac actatttagc ttcttcgcta agagcactct
     2521 catcgacaag tacgacctaa gcaacttgca cgagatcgcc agcggcgggg cgccgctcag
     2581 caaggaggta ggtgaggccg tggccaaacg cttccaccta ccaggcatcc gccagggcta
     2641 cggcctgaca gaaacaacca gcgccattct gatcaccccc gaaggggacg acaagcctgg
     2701 cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg acaccggtaa
     2761 gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg
     2821 ctacgttaac aaccccgagg ctacaaacgc tctcatcgac aaggacggct ggctgcacag
     2881 cggcgacatc gcctactggg acgaggacga gcacttcttc atcgtggacc ggctgaagag
     2941 cctgatcaaa tacaagggct accaggtagc cccagccgaa ctggagagca tcctgctgca
     3001 acaccccaac atcttcgacg ccggggtcgc cggcctgccc gacgacgatg ccggcgagct
     3061 gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg agatcgtgga
     3121 ctatgtggcc agccaggtta caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga
     3181 cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc aagatccgcg agattctcat
     3241 taaggccaag aagggcggca agatcgccgt gtaataattc tagagtcggg gcggcccatg
     3301 tttaagggtt ccggttccac taggtacaat tcgatatcaa gcttatcgat aatcaacctc
     3361 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3421 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3481 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     3541 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     3601 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     3661 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     3721 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     3781 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     3841 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     3901 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgat
     3961 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     4021 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     4081 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4141 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     4201 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     4261 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     4321 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     4381 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     4441 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     4501 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     4561 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     4621 ttttagtcag tgtggaaaat ctctagcagc atgtgagcaa aaggccagca aaaggccagg
     4681 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4741 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4801 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4861 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4921 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4981 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5041 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5101 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5161 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5221 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5281 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5341 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5401 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     5461 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     5521 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     5581 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     5641 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     5701 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     5761 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     5821 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     5881 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     5941 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6001 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6061 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6121 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6181 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6241 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6301 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6361 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6421 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     6481 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     6541 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     6601 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     6661 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
     6721 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     6781 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     6841 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     6901 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     6961 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     7021 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     7081 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     7141 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     7201 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     7261 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
     7321 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7381 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7441 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     7501 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     7561 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     7621 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     7681 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     7741 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     7801 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     7861 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     7921 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     7981 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
//
