LOCUS       sequence-306418-       14249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by 
            a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As 
            bright as conventional GCaMP7f..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14249)
  AUTHORS   Shemesh OA, Linghu C, Piatkevich KD, Goodwin D, Celiker OT, Gritton 
            HJ, Romano MF, Gao R, Yu CJ, Tseng HA, Bensussen S, Narayan S, Yang 
            CT, Freifeld L, Siciliano CA, Gupta I, Wang J, Pak N, Yoon YG, 
            Ullmann JFP, Guner-Ataman B, Noamany H, Sheinkopf ZR, Park WM, Asano
            S, Keating AE, Trimmer JS, Reimer J, Tolias AS, Bear MF, Tye KM, Han
            X, Ahrens MB, Boyden ES
  TITLE     Precision Calcium Imaging of Dense Neural Populations via a 
            Cell-Body-Targeted Calcium Indicator.
  JOURNAL   Neuron. 2020 Jun 26. pii: S0896-6273(20)30398-6. doi: 
            10.1016/j.neuron.2020.05.029.
  PUBMED    32592656
REFERENCE   2  (bases 1 to 14249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2020 Jun 26. pii: S0896-6273(20)30398-6. doi: 
            10.1016/j.neuron.2020.05.029."
COMMENT     Sequence Label: elavl3:SomaGCaMP7f
FEATURES             Location/Qualifiers
     source          1..14249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     regulatory      9455..9464
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9461..9469,9470..9487,9488..9490,9491..9523,9524..9526,
                     9527..9550,9551..9577,9578..9634,9635..9640,9641..9910,
                     9911..9928,9929..10363,10364..10369,10370..10810)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKELFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      9557..9566
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     9847..9868
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9974..9995)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10235..10254)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    11206..11340
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11230..11249)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11284..11303
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(11779..11966,11967..11978)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(12012..12028)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12012..12028)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12065..12085)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12065..12083)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12104..12120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12104..12120)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12117..12139)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12128..12144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12152..12158,12159..12176,12177..12182))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12197..12218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12335..12352)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12506..13094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12586..12605)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13265..14056,14057..14125))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13888..13907
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14126..14230)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttcccg
      721 ggtgtcgacg aattctcgag ctattcctaa agaccttggg tgaccaaaat cttattttaa
      781 taaataaaac tgtttattaa aacttttttg tttcaaagaa ccatatgtat agtgaaattt
      841 ataaaaatat caatttttaa aaagctggtg tactcattta tgttatgaac tctaaaacca
      901 tatactgact gcaagtgatg atgtatagag tgatgtttac gagtaaacat atttagttgt
      961 atacatccta ctgagcacat tttgatgtat gaaataacat tacaagcttt atccaaatta
     1021 agccatttta aaacactgcc aattgaaaat acaaatcctg gaaaaaatcg tctttagcgc
     1081 agtcatttga gccatcctaa tccgttacct cagaccataa taagaaggga taacactagc
     1141 tgtagcaatg gaacacatct gtttcacaca atcatatctc ctgcgccggt gctaagcaga
     1201 ttcagcgtga tcataacatg ctttccactc ataaatgtaa atttacaatt tgcacatgta
     1261 aaacagacac ttttgagata ttggataaaa aaacaagagt atattgctta gtttcatcca
     1321 ccagtcatcc ccacagcgtt tggaaggcca taaaaagtgt ctaaaatcaa tgatcattga
     1381 aagagcacaa gagagactct tacgctgtaa tgccactggg gacaaaagtg acagtctctt
     1441 aatgggctct tctggagggg ctcctgaaca ttaaaaatta tcagcgaaat taccgaaaga
     1501 gcttcaagca actggcatgc ttgatcctct gcgtcggggc ggtgaatagg tgcttcagat
     1561 gccctcttac ccacgggctg gattcagctg ccccgctacc agcggagacc ccctaatgag
     1621 cctctgcaat taagtttatt catgttaagt gtgaacgggg tgcgtgcgga actgtgggca
     1681 gctaacagac ctgggttctt tgtgccacaa gtgctgcctt tattcggctc acaaagcaga
     1741 aaacaacacc cgcacctata atggcgccct cggctgggtc taagaaacgt ggcgagttga
     1801 cagagcagag tgggcggggt taagacagac tgacagcggg acccatctcc atcctcttat
     1861 taacgcttaa cgagtgcctt cctcatgcaa tattcatcgc cactaatatc atccaagctc
     1921 tgagctgagc tggccactta tgtaaggcaa ttatgtaaaa tatcagacag ggcccacact
     1981 cagaatctga ctggggtaga gacgcgggac gagaaccgag agcaagaact gaaagtgaaa
     2041 gtgaccacta aagggaggag aggacagagg ggcaggatgt gtcaagatta ccagagaaca
     2101 cttggccaga aatgcgcaac cattggagct ctccggatta cccaaaggtt aacgagtttg
     2161 aacgcctctg cccactcgcc catctctgat ggtttcccaa gaactcctca agcaaaatat
     2221 atataattgt gtgtattatg cacagacacg agaaaatgct gtttttctga tctgcattac
     2281 agcacatttg cccgccaacg acaataccac ccactcggta cctcgctgac tcctgatgcc
     2341 tgatacctgc gcggtgactg tctacaatct gcataatcaa gagaagttgt gttgaagacg
     2401 agcgccacac aaccgtttcc acaaggtcac ccaaggccgg tgcagatgta ggtgaggtct
     2461 ccataaacag actgaaataa acacatcctc cgctgggaac aacaaccccc tcacgcctca
     2521 tgcatttcca taagcctaca tgcatctctt ccaacttatg gagactcgca cctaccaaca
     2581 tccgcacaac aaagatatac agagcgcgct ccctcaggtc aaggccctgt gggggtctgt
     2641 gcagaaatag gtcatttgtc acacatcaag tcctggggca ggagatgcat tatagatgag
     2701 accaaacagc ctgtctcggt gagctctacc cactccctga gactagaaat gggggaaggg
     2761 agcttgagat aacaaccgct gcaatcactg tgtcgatgtt taatatcagc accaaccggg
     2821 aacaataagg agatgggtgc attcatgttc acatcttacc agtcaagtat catcgaaccg
     2881 gcttgataac cacacctcgt gtaatagctg agcagatagt tgtcatttta aagcgttggc
     2941 ctttgtcgat tatgtaatgc gcacattcaa cacatggtaa tatagaaacg gttatgtcga
     3001 ggttgttttg tccagagatg accttcacac agttacagcc gctctgcatc cacacaaatg
     3061 gaggacttaa tcgtggactg cattcttaga aatgatctac aaagacaaat aatgtgaaat
     3121 caagaaagga caaaatttaa gtaaggggat gagggagaga gagaacgagg ggcaaggaga
     3181 aagcatggct cctgtctttt tctgcaccca tctgttcgga gtgcaggtgg agctctattc
     3241 actcagctct gcatgtgtgt ttgggggggg caggaagaaa gggagggcaa aaggaagagt
     3301 ggagagatgg tgggggctgg agggatgggg ggttctcggt gatctctcct gaaggggata
     3361 atgggagagc agcgctttgc aatggctgcc atgtagtacc ctccctgcac aattagccaa
     3421 tcagcagcaa gctctgccag ccagaaggac acataaaaga agaacattgc agcagaggca
     3481 cagaaggagc ctgcgaggag ctgggaaata cacacacaac agcagaacca caacaccctc
     3541 ccctggacac accctactgg ggatcactgc ttttcttttt ttctgaacca tcgcccacgc
     3601 cacacggaga gaaatctctc tctcatcatc atcctgaaga aaaccccctt atcctcattt
     3661 tcacactgct gaggaaaacc tacaatcgca cgggctgaga tttcctggcg aagactgtcc
     3721 tttttccttt ttcttttttt ttcttttcct ttggaaactg acatttgcat ttctccattc
     3781 caagccacgg cgtaataata tctgcaatcc agcctgaaga cctgcaaatc gaaggaccta
     3841 gatcactatc atctttgtac gtcaagaatg gttactgtac gtataacctt tctttctttt
     3901 gctctgacca atatgaaaca ctaaaatcga ttcgagcagc ctctcagcat caattacagt
     3961 gcgtgaaaaa cattcaaata gaggcagcaa atattacatg tgaaaataca ggctggctaa
     4021 atccaagcta atttagaaat gtggtcaaaa cgcatactgg cacgtctaat cgcggattca
     4081 gtaaacaaga ttaacgatta gcccagtgta taagtcatat gatacaggca tgcgcgagag
     4141 catcgctaca cccgagctgg cttcattttc ggaggaaaat caaaacattg ctttctcctg
     4201 ccgtgcgaac cattcgtcat aaaccgtaat acgcaacata catttattac tacatccgtt
     4261 aattagcgat aattagccgt tattaacaaa gagcgctgag gaattcctct caaatagcgg
     4321 aggtgcggcg gaggcagagg ggcgtaaaag ggcacatgcg tgcctggctc aaaaaaggat
     4381 gctccagact gaaactcaga gacaaaacac gatgcctcgg aggctgagag cccatcgaga
     4441 ggaaaggcaa agaaaagggt cgagtcactc agagggggag gggagatatt gtgcatccat
     4501 tggtttgaat tgaagcaggc agaataaaaa tccttgcgat tatgcttcgt gagagatcgc
     4561 gagagaggaa aaggctaaat gccacgtatg caaatggata aatgtcatct atttcttcgc
     4621 gagtagacat tttgtggaca acgagagtgt aaataagcga tatgagggga atacaaaggc
     4681 cttgagggaa agcgttttgg gcgtgtcttg tcgaaaagaa gacagcgatg gcacgcgctc
     4741 agaaccacca tcttcacgta gataatccgc gtgaaaacaa taacaaaact gctcatttta
     4801 acacgagcag gtgtttgaaa ccacccaaaa aagattgtct aaaatattaa gattaattaa
     4861 tgacgtttaa atgtccaggc ctgatataaa tgaagcgtgt agtaagagta atcgcctttg
     4921 tttcttttca gagatacgtt ttcagagatt cagggaatta tatcttcaat aaaataatgt
     4981 ttgatttcat tattttaaag ctttttaaga agtaaaaata cacattattc atttacattt
     5041 tattttattt aaaggcctat ggtcttgaaa ataataagtg gaatatatag gctatatatt
     5101 cgtctaagga cagccggtgt tattttatag tgacctcaga caaaagccga gacaaaacga
     5161 ggcgcttgcc tttcatttta atccttaata gacggtgtga tgaatgaatg aatgaatgaa
     5221 taaatgaatg aatgacgggc ccttcggcat gttgttccgc agtcctccgt gagcacgagt
     5281 ctctaccgca gtgccaagca cataaaaccg ttgagtcaga taaccttctc caggcgcgta
     5341 tacacaattc aaggacaagc acatcacaac tatacacaac acaacacaac cgcctaaacg
     5401 cacgagctca tcaatcgcaa tttaaaggca tgtcagtcaa agaaaaaggc ggcgttattt
     5461 aaaaccaatg ttcaacaata tttgtatgct gactagcccg ggcgcgcacg ctataagatc
     5521 acaaggcctt gttttttccc tttattcaaa atatcattaa ttattacagg tcataaaaca
     5581 aaacaatgca aattacaatg atttaaagac tattgattta acaataatca gttaattata
     5641 gaataatatt ataatatttt acatacaaat gaaagatgac attatttgat gacattgtaa
     5701 ttaaaatata tgcaatatat ttttacgttt ttattaatta ggctattact attattataa
     5761 ttatcattat ttttattatt attattatta ttaaatagtc ttgcgaaata agagtgaagg
     5821 cagttgcaat gttcattgtt cccggagagt cccgcagcct ctggcctcaa cacaaagcgc
     5881 tactgtttac attaatattc atagtgctgc catggtctct gtagaggagg aacgggtggc
     5941 tcttttgttc gctagtgtca atacgtttat tcagcaaaaa gcagctcgga tttgtgaatg
     6001 cccaacaagc acacgcgcac tccaatcaaa ggacgagagg ataacgattt taaacgattc
     6061 gcaaacgtgt ttatttgtaa agacgacatc aagagcttaa tgtccacttg aaaagaaata
     6121 aatatgcccg cggcgccaag gatattttga atgtagtcgt tcatggtgag ccactcccga
     6181 attcagcact ctggagagcg accaaagagg aatagcggaa tagcaactac ttgagccatt
     6241 tctctccgtt tgatggcgtt catgattaaa aatataatca cgtgattttt tatacatata
     6301 taaatatata cataaagtaa cgctttcctt ttgagaatac ataaatattt attcgcgtga
     6361 taaatcaatg atcttatatt tatttgcacg accgaaatta gccaaattca ccaattaaaa
     6421 aaaaaataag ccaacaaaaa agaagggttt atccgttcag ttttgacttg tgtcctgttg
     6481 ttttacagct gccactgtga tcccttaagc tgcagtgaga gtgtggctaa tgccttttgt
     6541 ttaagataat cctgtaatct gttaccgaaa cggcctattg acaagccggc attcacattt
     6601 cagtcaggag gcccgtccag acatgtacat ttatgaatta cgaatgataa aattaagatc
     6661 tgcattaggc gtcagaaatg tcacgaacac ccattcattt cccacagcca ataatggatc
     6721 atgctgggaa gcgctatcct cgcagtctca aaattaatgt tgacatgttg cgtagagcta
     6781 cagattagat cagccatatg ctttatgtgt ttacctcagc ggatcttcag caagatgtgt
     6841 ttattttaag aaaatggctt cctcgctgcc tgcacagggg cattaaggaa gtgacgtgag
     6901 cgtcctcaca gtcaaattac tttatctcat gctgctttca ggcctctgct tattttatta
     6961 ttattatttt aaattagggg gaatcacggt ggcgttgtgg gtagggtagc gcgatcacct
     7021 tacagcaaga aggtcgctgg ttcgagctct ggctgggtca gttggcattt ctgtgtggag
     7081 tttaaattga attgaaattg aataaactaa attggcccta gtgtatgtgt gtgaatgtaa
     7141 gtgtgcatgg gtgtttccca gtgttgggtt tgactggaag agcatccgct tcgtaaaaca
     7201 tatgctggat aagttggcgg ttcatttcac tgtggtgacc gctgattaat aaagggacta
     7261 agctgaaaat gaaatgaatt aatacatttt aaatgagctt ttgctgacat gtttatgatt
     7321 acaaatagtg aaattgtatg ttaatttacg ataagtgcat tgttctaaat tcatttttat
     7381 gtagcagctg gtttttcttc catttgtgtc attcaacaga acatttttct aaaaaagtga
     7441 acttaaattt tctcctccct ctgacaaggc ttgatggtaa tttcagccga ctctaattac
     7501 agactcacta aaaggaatcg gtgcagtcat ttatctttat cagcgcgctg gtgggaaggt
     7561 aataggtttt gcttatgagt tgtttgcgtg cgagtctggg tccttgtgta ggcgatgctg
     7621 atgcactgca ttaaatattg agggagaagt cgctcaaaaa tagcaaagta caggctgggg
     7681 caggaacgtc aaagcccgct cagtggccac gtttcttgtt tcgtaagtcc tatttttaat
     7741 agcgataatt caggctcagt agaaggaact ccagcactat aagcagtctc tcgcctgtct
     7801 cggcgagtta tgtattttgg gatgaagtgc tcttccacct ctacctctgc tgtgctggcg
     7861 ccagtatctg ggctgtaaga tagtgtacgt gtcagtctgt gtggccgaag agcctgtgtg
     7921 ttgtgtaact cactacattc gtcgctgtgg atggctgact tgccatgggg aggtttactt
     7981 cagttcacga atcacattcg ccacgatctt gtcactttgt gtcaagactc gctctctgtg
     8041 tgtcaacagc tctgctgttt gaggttgaaa ttctgaactt gagatatatc ccaggatata
     8101 ttagggctac tgccaacgga aaaagcaaat ggtgccatgg ccatttgatt gatcaaacaa
     8161 acaccctttt gcttgggtga cagtgaaatg tcagatccat gtttgtactg ctttaataac
     8221 ttgcctctct gagctttctc cttgtcagtg tcaatttgcc tgagatataa attccggttg
     8281 ccccgaaagc ttattttatt aaaaaaaagc taacatgcac tcatacaacg tgcgcacata
     8341 catgtgaggc ttgctaaaag tcaagaagca agccaatagc aagagtcgtc agactgctgt
     8401 tacctgggca acctggttag aggtaaggct caaggcaaga ataaatcatt ccactgaaga
     8461 gacacagcgt tacagcgaga tgagagcaag aggcgatagt gaagggagaa caaaagaaca
     8521 gcctacccac actagtgcag atctacctgt ttgagctact gattgttcag tttgattaga
     8581 tatcagacaa ttctgctatc tccaattgca ttgctgcatg aattcctaga aatcatttta
     8641 atcaccaaaa actagtaggg acacttgtgg cgtatatgat tctgcctgtt gattgtgggg
     8701 atccaacaga aagatcatga atgcattgtt aattaagtcg gtgagacacc ctgttccacc
     8761 ctaggggccg ttctggggaa agagtgcttt cagtcagcta aagatgacta atatgtgaac
     8821 atatattact acacatgcca atttgtacat tctggatagt taccagccct gggaaaacat
     8881 cgaaaaaaat aaatcaacat agataaaatt ggcaaatccc ttctggtacg atgcagagtg
     8941 cttgctctga gaacttgtgc attgtgacag aaagcgaccg ttaaaaactg aaagtagcat
     9001 ctacaacctg catacatctg ttctttgaaa atcccctgta ggccaaatta agcatcacgc
     9061 tgtgctccac agcaacacgg atgaaccgaa aacccagaca caaacgcaca cacttttcac
     9121 ataaacaagg tttagttatg ctagctatgc tttttcgtcc tccctttgtc atcagcggcg
     9181 agtgacgtaa cacagtttga ccctggacag cgtaaagggg gggttgggaa tgagtgaaag
     9241 gactgtgaca tctgcagggc caggggctga tggagagagc tgaagtgagg gaggtcagag
     9301 gaggaggaag ggtgggatat tgtcctcctc aggccctatc cctctcagac gcattgattt
     9361 ttaacctctc agcgagaatg ccaagcatta cacccctcaa attatggttc ctcacccaac
     9421 ctgttatatt ttccacctgc agataattag gcgcgccacc atgggttctc atcatcatca
     9481 tcatcatggt atggcttcca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga
     9541 tgacgataag gatctcgcca ccatggtcga ctcatcacgt cgtaagtgga ataagacagg
     9601 tcacgcagtc agagctatag gtcggctgag ttcactggag aacgtctata tcaaggccga
     9661 caagcagaag aacggcatca aggcgaactt caagatccgc cacaacatcg aggacggcgg
     9721 cgtgcagctc gcctaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     9781 gcccgacaac cactacctga gcgtgcagtc caaactttcg aaagacccca acgagaagcg
     9841 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     9901 gctgtacaag ggcggaaccg gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg
     9961 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
    10021 cggcgagggt gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
    10081 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
    10141 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
    10201 aggctacatc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
    10261 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
    10321 caaggaggac ggcaacatcc tggggcacaa gctggagtac aacctgccgg accaactgac
    10381 tgaagagcag atcgcagaat ttaaagagct gttctcccta tttgacaagg acggggatgg
    10441 gacaataaca accaaggagc tggggacggt gatgcggtct ctggggcaga accccacaga
    10501 agcagagctg caggacatga tcaatgaagt agatgccgac ggtgacggca caatcgactt
    10561 ccctgagttc ctgacaatga tggcaagaaa aatgaaatac acagacagtg aagaagaaat
    10621 tagagaagcg ttccgtgtgt ttgataagga tggcaatggc tacatcagtg cagcagagct
    10681 tcgccacgtg atgacaaacc ttggagagaa gttaacagat gaagaggttg atgaaatgat
    10741 cagggaagca gacatcgatg gggatggtca ggtaaactac gaagagtttg tacaaatgat
    10801 gacagcgaag ggaggttctg gaggttctgg tggtactggt ggttctggag gatctggtgg
    10861 tacaggaggt agcggaggtt ctggtggtac cctggaaatc gaggccgctt tcctggagca
    10921 ggagaacacc gccctggaga cagaggtggc tgagctggag caggaggtgc agagactgga
    10981 gaacatcgtg tctcagtacg agaccagata cggaccactg ggatccctgg agatcagagc
    11041 cgcttttctg aggagaagaa acaccgccct gaggacaagg gtggctgagc tgaggcagag
    11101 agtgcagaga ctgcgcaaca tcgtgagcca gtacgagaca cgctacggac ctctgtaagt
    11161 ttaaacggtg gatcctggcc gtcgttttac tgtaccatcg atgatgatcc agacatgata
    11221 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
    11281 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
    11341 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
    11401 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct agatcagatc
    11461 tgcgaagata cggccacggg tgctcttgat cctgtggctg attttggact gtgctgctcg
    11521 cagctgctga tgaatcacat acttcctcca ttttcttcca ctgattgact gttataattt
    11581 ccctaatttc caggtcaagg tgctgtgcat tgtggtaata gatgtgacat gacgtcactt
    11641 ccaaaggacc aatgaacatg tctgaccaat ttcatataat gtgaaaacga ttttcatagg
    11701 cagaataaat aacatttaaa ttaaactggg catcagcgca attcaattgg tttggtaata
    11761 gcaagggaaa atagaatgaa gtgatctcca aaaaataagt actttttgac tgtaaataaa
    11821 attgtaagga gtaaaaagta cttttttttc taaaaaaatg taattaagta aaagtaaaag
    11881 tattgatttt taattgtact caagtaaagt aaaaatcccc aaaaataata cttaagtaca
    11941 gtaatcaagt aaaattactc aagtacttta cacctctggt tcttgacccc ctaccttcag
    12001 caagcccagc agatccacta gttctagagc ggccgccacc gcggtggagc tccagctttt
    12061 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
    12121 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    12181 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    12241 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    12301 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    12361 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    12421 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    12481 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    12541 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    12601 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    12661 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    12721 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    12781 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    12841 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    12901 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    12961 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    13021 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    13081 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    13141 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    13201 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    13261 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    13321 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    13381 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    13441 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    13501 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    13561 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    13621 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    13681 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    13741 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    13801 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    13861 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    13921 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    13981 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    14041 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    14101 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    14161 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    14221 gggttccgcg cacatttccc cgaaaagtg
//
